{"id":42482,"date":"2023-05-18T17:28:54","date_gmt":"2023-05-18T09:28:54","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42482"},"modified":"2023-11-13T15:17:32","modified_gmt":"2023-11-13T07:17:32","slug":"tunel%e7%b4%b0%e8%83%9e%e5%87%8b%e4%ba%a1%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e6%a9%99%e8%89%b2%e8%9e%a2%e5%85%89%ef%bc%89-one-step-tunel-apoptosis-assay-kit%e8%b2%a8","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=42482","title":{"rendered":"TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6a59\u8272\u87a2\u5149\uff09 |\u00a0 One-step TUNEL Apoptosis Assay Kit\u8ca8\u865f KTA2011"},"content":{"rendered":"

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6a59\u8272\u87a2\u5149\uff09 |\u00a0 One-step TUNEL Apoptosis Assay Kit\u8ca8\u865f KTA2011<\/span><\/a><\/span><\/strong><\/p>\n

One-step TUNEL Apoptosis Assay Kit (Orange Fluorescence)<\/span><\/p>\n

\u51cb\u4ea1\u7d30\u80de(apoptotic cells)\u6700\u5bb9\u6613\u6e2c\u91cf\u7684\u7279\u5fb5\u4e4b\u4e00\u662f\u57fa\u56e0\u7d44 DNA ( genomic DNA)\u88ab\u7d30\u80de\u6838\u9178\u9176(cellular nucleases)\u5206\u89e3\u3002\u672b\u7aef\u812b\u6c27\u6838\u82f7\u9178\u8f49\u79fb\u9176 dUTP \u7f3a\u53e3\u672b\u7aef\u6a19\u8a18 (Terminal deoxynucleotidyl transferase dUTP nick end labeling, TUNEL) \u662f\u4e00\u7a2e\u901a\u904e\u6a19\u8a18\u7d30\u80de\u51cb\u4ea1\u904e\u7a0b\u4e2d\u7522\u751f\u7684\u96d9\u93c8 DNA( DNA fragmentation) \u65b7\u88c2\u4e2d\u7684 3′-\u7fa5\u57fa\u672b\u7aef(3\u2032-hydroxyl termini)\u4f86\u6aa2\u6e2c DNA \u7247\u6bb5\u5316(DNA fragmentation )\u7684\u65b9\u6cd5\u3002 TUNEL \u6e2c\u5b9a\u4f9d\u8cf4\u65bc DNA \u4e2d\u5b58\u5728\u7684\u7f3a\u53e3\uff0c\u53ef\u4ee5\u901a\u904e TdT \u8b58\u5225\uff0cTdT \u662f\u4e00\u7a2e\u50ac\u5316\u6dfb\u52a0\u87a2\u5149\u7d20\u6a19\u8a18\u7684 dUTP \u7684\u9176\u3002<\/p>\n

<\/p>\n

Fig. Fluorescence images using Abbkine TUNEL Apoptosis Detection Kit (Orange Fluorescence) in HeLa cells with the treatment of 10 U\/mL Dnase I for 10min.<\/span><\/p>\n

Abbkine \u55ae\u6b65 TUNEL \u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6a59\u8272\u87a2\u5149\uff09\u4f9d\u8cf4\u65bc DNA \u4e2d\u7f3a\u53e3\u7684\u5b58\u5728\uff0c\u8a72\u7f3a\u53e3\u53ef\u901a\u904e TdT \u8b58\u5225\uff0cTdT \u662f\u4e00\u7a2e\u50ac\u5316\u6dfb\u52a0\u87a2\u5149\u7d20\u6a19\u8a18\u7684 dUTP \u7684\u9176\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u6240\u6709\u57fa\u672c\u7d44\u4ef6\u548c\u512a\u5316\u7684\u6aa2\u6e2c\u65b9\u6848\uff0c\u9069\u7528\u65bc\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002\u5b83\u7684\u4fe1\u865f\u5f88\u5bb9\u6613\u88ab\u6aa2\u6e2c\u5230\uff08Ex\/Em = 555nm\/565nm\uff09\u3002<\/p>\n

<\/p>\n

Specification<\/span><\/span><\/h3>\n\n\n\n\n
Product name<\/td>\nOne-step TUNEL Apoptosis Assay Kit (Orange Fluorescence)<\/a><\/td>\n<\/tr>\n
Applications notes<\/td>\nAbbkine One-step TUNEL Apoptosis Assay Kit (Orange Fluorescence) relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein. This kit provides all the essential components with an optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer. Its signal can be easily detected ( Ex\/Em = 555nm\/565 nm).<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Properties<\/span><\/strong><\/span><\/h3>\n\n\n\n\n\n\n\n\n
Kit components<\/td>\n\u2022 TdT Enzyme
\n\u2022 Equilibration Buffer (5\u00d7)
\n\u2022Label Mix Orange
\n\u2022DAPI (500\u00d7)
\n\u2022BSA Working Solution
\n\u2022TritonX-100 (100%)
\n\u2022DNase I (5 U\/\u00b5L)<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Optimized assay protocol, suitable for fluorescence microplate reader, fluorescence microscope, or flow cytometer.
\n\u2022 Signal can be easily detected (Ex\/Em = 555nm\/565 nm).<\/td>\n<\/tr>\n
Usage notes<\/td>\nThe reaction buffer contains cacodylate and cobalt chloride, highly toxic chemicals. After contact with skin, wash immediately with plenty of water. In case of accident or if you feel unwell, seek medical advice immediately. Do not drink, eat or smoke when using.<\/td>\n<\/tr>\n
Storage instructions<\/td>\nStable for at least 6 months at -20\u00b0C from date of shipment. Once opened, refer to list of materials supplied for storage conditions of individual components.<\/td>\n<\/tr>\n
Shipping<\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Additional Information<\/span><\/strong><\/span><\/h3>\n\n\n\n
Background<\/td>\nOne of the most easily measured features of apoptotic cells is the break-up of the genomic DNA by cellular nucleases. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) is a method for detecting DNA fragmentation by labeling the 3\u2032-hydroxyl termini in the double-strand DNA breaks generated during apoptosis. The TUNEL assay relies on the presence of nicks in the DNA which can be identified by TdT, an enzyme that catalyzes the addition of dUTPs that are labeled with fluorescein.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/p>\n

A.\u6a23\u54c1\u88fd\u5099<\/strong><\/span><\/p>\n

—-\u7528\u65bc\u8cbc\u58c1\u7d30\u80de(adherent cells)\uff08\u87a2\u5149\u986f\u5fae\u93e1\u5206\u6790\uff09<\/p>\n

    \n
  1. 96-well microplate culture \u751f\u9577\u81f3\u5c1124h\u3002\u901a\u904e\u8a98\u5c0e\u7d30\u80de\u51cb\u4ea1\u6240\u9700\u7684\u65b9\u6cd5\u3002\u540c\u6642\u57f9\u990a\u7121\u8a98\u5c0e\u7684\u5c0d\u7167\u7d44\u3002<\/li>\n
  2. \u79fb\u53bb\u57f9\u990a\u57fa\uff0c\u4e26\u5728PBS\u4e2d\u75284\uff05\u591a\u805a\u7532\u919b(4%paraformaldehyde)\u56fa\u5b9a\u7d30\u80de\u5ba4\u5167\u6eab\u5ea630\u5206\u9418\u3002<\/li>\n
  3. \u9664\u53bb\u56fa\u5b9a\u6db2\u4e26\u7528PBS\u6e05\u6d173\u6b21\uff08\u6bcf\u6b215\u5206\u9418\uff09\u3002<\/li>\n
  4. \u56fa\u5b9a\u5f8c\uff0c\u6dfb\u52a0\u901a\u900f\u5291\uff0850\u00b5L 0.3% TritonX-100\uff0c\u672a\u63d0\u4f9b\uff09\uff0c\u4e26\u5c07Plate\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b230\u5206\u9418\u3002\u7528BSA Working Solution\u6d17\u6ecc\u7d30\u80de2-3\u6b21\u3002\u7e7c\u7e8c\u57f7\u884c\u6b65\u9a5fB 1\u3002<\/li>\n<\/ol>\n

    \u6700\u4f73\u5316: \u88fd\u5099TUNEL\u53cd\u61c9\u7684\u967d\u6027\u5c0d\u7167( positive control): \u5ba4\u6eab\u4e0b\u5c07\u542b\u670910 U \/ mL DNAase I\u7684\u7d30\u80de, \u5728\u5ba4\u6eab\u4e0b\u653e\u7f6e10\u5206\u9418\uff0c\u7136\u5f8c\u518d\u9032\u884cTUNEL\u64cd\u4f5c\u53cd\u61c9\uff08\u6b65\u9a5fB 1\uff09\u3002<\/p>\n

    —-\u7528\u65bc\u975e\u8cbc\u58c1\u7d30\u80de \uff08\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u5206\u6790\uff09<\/p>\n

      \n
    1. \u57f9\u990a\u7d30\u80de\u81f3\u6700\u4f73\u5bc6\u5ea6\uff08\u7d041-2 x 106<\/sup>\u7d30\u80de\/ mL\uff09\u3002\u901a\u904e\u8a98\u5c0e\u7d30\u80de\u51cb\u4ea1\u6240\u9700\u7684\u65b9\u6cd5\u3002\u540c\u6642\u57f9\u990a\u7121\u8a98\u5c0e\u7684\u5c0d\u7167\u57f9\u990a\u7269\u3002<\/li>\n
    2. \u901a\u904e300g\u96e2\u5fc3\u6536\u96c61-5 x 106<\/sup>\u500b\u7d30\u80de\u3002\u75280.5 mL PBS\u6d17\u6ecc\u5169\u6b21\u3002<\/li>\n
    3. \u52a0\u51651 mL 4\uff05\u591a\u805a\u7532\u919b\uff08\u5728PBS\u4e2d\uff0cpH 7.4\uff09\u4e26\u5728\u51b0\u4e0a\u5b75\u80b230\u5206\u9418\u3002<\/li>\n
    4. \u4ee5300g\u96e2\u5fc3\u7d30\u80de\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\uff0c\u91cd\u61f8\u65bc1 mL PBS\u4e2d\u3002\u91cd\u8907\u6b64\u6d17\u6ecc\u5169\u6b21\u3002<\/li>\n
    5. \u5728\u5ba4\u6eab\u4e0b\u5c07\u7d30\u80de\u5728500\u00b5L 0.3\uff05Triton-X 100\u4e2d\u91cd\u61f85\u5206\u9418\u4ee5\u901a\u900f\uff08\u6216\u8005\uff0c\u5c07\u7d30\u80de\u91cd\u61f8\u65bc100\u03bcg\/ mL\u86cb\u767d\u9176K\u4e2d5\u5206\u9418\u4ee5\u4f7f\u5176\u901a\u900f\uff09\u3002<\/li>\n
    6. \u4ee5300g\u96e2\u5fc3\u7d30\u80de\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\uff0c\u91cd\u61f8\u65bc1 mL PBS\u4e2d\u3002<\/li>\n<\/ol>\n

      \u91cd\u8907\u6b64\u6d17\u6ecc\u5169\u6b21\uff0c\u7136\u5f8c\u7e7c\u7e8c\u6b65\u9a5fB 2\u3002
      \n—–\u7528\u65bc\u77f3\u881f\u5305\u57cb\u7684\u7d44\u7e54\uff08\u901a\u904e\u87a2\u5149\u986f\u5fae\u93e1\u5206\u6790\uff09<\/p>\n

        \n
      1. \u901a\u904e\u5c07\u5169\u6b21\u5728\u4e8c\u7532\u82ef\u4e2d\u6d78\u6ce110-20\u5206\u9418\u4f86\u5c0d\u7d44\u7e54\u9032\u884c\u812b\u881f\u3002<\/li>\n
      2. \u901a\u904e\u4ee5\u4e0b\u6d17\u6ecc\uff08\u7d66\u5b9a\u9806\u5e8f\uff09\u70ba\u7d44\u7e54\u88dc\u6c34\uff1a\u5169\u6b21\u6d17\u6ecc5\u5206\u9418
        \nc. \u6bcf\u6b21\u5728100\uff05\u4e59\u9187\u4e2d\u6d17\u6ecc\uff0c\u7136\u5f8c\u4f9d\u6b21\u572895\uff05\uff0c70\uff05\u548c50\uff05\u4e59\u9187\u4e2d\u6bcf\u6b21\u6d17\u6ecc3\u5206\u9418\u3002<\/li>\n
      3. \u7528PBS\u6d17\u6ecc\u6a23\u54c1\u5169\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\u3002<\/li>\n
      4. \u6392\u51fa\u7d44\u7e54\u4e2d\u591a\u9918\u7684PBS\uff0c\u4e26\u572820 \u00b5g \/ mL\u86cb\u767d\u9176K\u4e2d\u5b75\u80b215\u5206\u9418\uff08\u5728PBS\uff09\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n

        \u6ce8\u610f\uff1a\u86cb\u767d\u9176\u6d88\u5316\u7684\u6642\u9593\u5fc5\u9808\u91dd\u5c0d\u7279\u5b9a\u7684\u7d44\u7e54\u985e\u578b\u548c\u689d\u4ef6\u9032\u884c\u512a\u5316\u3002\u539a\u5ea6\u3002\u86cb\u767d\u9176\u904e\u5ea6\u6d88\u5316\u5c07\u5c0e\u81f4\u7d30\u80de\u7d50\u69cb\u55aa\u5931\uff0c\u4e26\u53ef\u80fd<\/p>\n

        \u5f9e\u73bb\u7247\u4e0a\u91cb\u653e\u7d44\u7e54\u5207\u7247\u3002\u6d88\u5316\u4e0d\u826f\u6703\u5c0e\u81f4TdT\u6a19\u8a18\u4e0d\u826f\u3002\u4f7f\u7528\u524d\u65b0\u9bae\u7684\u86cb\u767d\u9176\u6eb6\u6db2\u3002<\/p>\n

          \n
        1. \u901a\u904e\u5728PBS\u4e2d\u5c07\u7d30\u80de\u6d17\u6ecc3\u6b21\u6bcf\u6b215\u5206\u9418\u4f86\u7d42\u6b62\u86cb\u767d\u9176\u8655\u7406\u8f15\u8f15\u5730\u652a\u52d5\u3002\u7e7c\u7e8c\u57f7\u884c\u6b65\u9a5fB 1\u3002<\/li>\n<\/ol>\n

          —–\u7528\u65bc\u51b7\u51cd\u7d44\u7e54\u5207\u7247\uff08\u87a2\u5149\u986f\u5fae\u93e1\u5206\u6790\uff09<\/p>\n

            \n
          1. \u5207\u7247\u5728\u5e7b\u71c8\u7247\u4e0a\u4e7e\u71e5\u5f8c\uff0c\u7528PBS\u4e2d\u76844\uff05\u591a\u805a\u7532\u919b\u56fa\u5b9a30\u5206\u9418\u5728\u5ba4\u6eab\u4e0b\u3002<\/li>\n
          2. \u6d78\u5165PBS\u5169\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\uff0c\u4ee5\u9032\u884c\u6d17\u6ecc\u3002<\/li>\n
          3. \u6392\u51fa\u7d44\u7e54\u4e2d\u591a\u9918\u7684PBS\uff0c\u4e26\u572820 \u00b5g \/ mL\u86cb\u767d\u9176K\u4e2d\u5b75\u80b215\u5206\u9418\uff08\u5728PBS\uff09\u6eb6\u6db2\u3002<\/li>\n
          4. \u901a\u904e\u5728PBS\u4e2d\u5c07\u7d30\u80de\u6d17\u6ecc3\u6b21\u6bcf\u6b215\u5206\u9418\u4f86\u7d42\u6b62\u86cb\u767d\u9176\u8655\u7406\u8f15\u8f15\u5730\u652a\u52d5\u3002\u7e7c\u7e8c\u57f7\u884c\u6b65\u9a5fB 1\u3002<\/li>\n<\/ol>\n

            B. Tunel\u5206\u6790<\/span><\/p>\n

            \u87a2\u5149\u986f\u5fae\u93e1\u5206\u6790<\/span><\/strong><\/span><\/p>\n

            <\/p>\n

              \n
            1. \u5728\u4f7f\u7528\u524d\u6839\u64da\u6a23\u54c1\u6578\u91cf\u6e96\u5099TdT\u6a19\u8a18\u53cd\u61c9\u7de9\u885d\u6db2\u88ab\u6aa2\u6e2c\uff1a\u5411\u6bcf\u500b\u6a23\u54c1\u4e2d\u52a0\u516550 \u00b5L\u53cd\u61c9\u6df7\u5408\u7269\uff08\u4f86\u81ea\u6b65\u9a5fA 1\u30013\u548c4\uff09\uff0c\u4e26\u5b75\u80b2\u572837 oC\u4e0b\u653e\u7f6e60\u5206\u9418\u3002<\/li>\n
            2. \u5c07\u6a23\u54c1\u5728PBS\u4e2d\u6d17\u6ecc3\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\u3002 \u901a\u904e\u57281 \u00b5g \/ mL\u7898\u5316\u4e19\u5576\u62160.05 \u00b5g \/ mL DAPI\u4e2d\u5b75\u80b2\u800c\u5c0d\u6a23\u54c1\u9032\u884c\u8907\u67d3PBS 10\u5206\u9418<\/li>\n<\/ol>\n

              \u6ce8\u610f\uff1a\u6839\u64da\u7d44\u7e54\u7684\u4e0d\u540c\uff0c\u53ef\u80fd\u5fc5\u9808\u8abf\u6574\u5fa9\u67d3\u5291\u7684\u6fc3\u5ea6\u3002\u7898\u5316\u4e19\u9320\u6216DAPI\u904e\u5ea6\u67d3\u8272\u53ef\u80fd\u6703\u5c0e\u81f4\u89c0\u5bdf\u87a2\u5149\u7d20\u6a19\u8a18\u3002<\/p>\n

                \n
              1. \u5c07\u6a23\u54c1\u5728PBS\u4e2d\u6d17\u6ecc3\u6b21\uff0c\u6bcf\u6b215\u5206\u9418\u3002<\/li>\n
              2. \u6dfb\u52a0\u6c34\u6027\u56fa\u5b9a\u4ecb\u8cea\u6216\u9632\u892a\u8272\u6eb6\u6db2\uff0c\u84cb\u4e0a\u84cb\u73bb\u7247\u4e26\u4f7f\u7528\u5e36\u6709\u7192\u5149\u7d20\u6ffe\u5149\u7247\u7684\u87a2\u5149\u986f\u5fae\u93e1\u9032\u884c\u5206\u6790\u3002<\/li>\n<\/ol>\n

                \u6d41\u5f0f\u7d30\u80de\u8853\u5206\u6790<\/strong><\/span><\/p>\n

                  \n
                1. \u5c07\u7d30\u80de\u91cd\u61f8\u65bc100 \u00b5L 1 x\u5e73\u8861\u7de9\u885d\u6db2\u4e2d\u3002\u5728\u5ba4\u6eab\u4e0b\u5b75\u80b210\u5206\u9418<\/li>\n
                2. 300g\u96e2\u5fc3\u7d30\u80de\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\u4e26\u91cd\u61f8\u65bc50 \u00b5L TdT\u4e2d\u6a19\u8a18\u53cd\u61c9\u7de9\u885d\u6db2\u3002\u572837oC\u4e0b\u5b75\u80b260\u5206\u9418\u3002\u5b9a\u671f\u8f15\u8f15\u5730\u6df7\u5408\u7d30\u80de\u3002<\/li>\n
                3. 300g\u96e2\u5fc3\u7d30\u80de\u3002\u9664\u53bb\u4e0a\u6e05\u6db2\uff0c\u91cd\u61f8\u65bc1 mL PBS\u4e2d\u3002\u91cd\u8907\u6d17\u5169\u6b21\u3002<\/li>\n
                4. \u91cd\u61f8\u65bcPBS\u4e2d\u76841 mL 1 \u00b5g \/ mL\u7898\u5316\u4e19\u5576\u62160.05 \u00b5g \/ mL DAPI\u4e2d\u3002\u5b75\u531610\u5206\u9418<\/li>\n
                5. \u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u5206\u6790\u7d30\u80de<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
                  Catalog No<\/td>\nProduct Name<\/td>\n<\/tr>\n
                  KTA0001<\/td>\nAnnexin V-AbFluor\u2122 405 Apoptosis Detection kit (Blue Fluorescence)<\/td>\n<\/tr>\n
                  KTA0002<\/td>\nAnnexin V-AbFluor\u2122 488 Apoptosis Detection kit (Green Fluorescence)<\/td>\n<\/tr>\n
                  KTA0003<\/td>\nAnnexin V-AbFluor\u2122 555 Apoptosis Detection kit (Orange Fluorescence)<\/td>\n<\/tr>\n
                  KTA0004<\/td>\nAnnexin V-AbFluor\u2122 647 Apoptosis Detection kit (Red Fluorescence)<\/td>\n<\/tr>\n
                  KTA1001<\/td>\nLive and Dead Cell Double Staining Kit<\/td>\n<\/tr>\n
                  KTA4001<\/td>\nMitochondrial Membrane Potential Assay Kit (JC-1)<\/td>\n<\/tr>\n
                  KTA1030<\/td>\nLDH Cytotoxicity Assay Kit<\/td>\n<\/tr>\n
                  KTA2010<\/td>\nTUNEL Apoptosis Detection Kit (Green Fluorescence)<\/td>\n<\/tr>\n
                  KTA2011<\/td>\nTUNEL Apoptosis Detection Kit (Orange Fluorescence)<\/td>\n<\/tr>\n
                  KTA2020<\/td>\nCell Cycle Staining Kit<\/td>\n<\/tr>\n
                  KTA2030<\/td>\nEdU Cell Proliferation Image Kit (Green Fluorescence)<\/td>\n<\/tr>\n
                  KTA2031<\/td>\nEdU Cell Proliferation Image Kit (Orange Fluorescence)<\/td>\n<\/tr>\n
                  KTA3020<\/td>\nCaspase-1 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA3021<\/td>\nCaspase-2 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA3022<\/td>\nCaspase-3 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA3023<\/td>\nCaspase-4 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA3024<\/td>\nCaspase-6 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA3025<\/td>\nCaspase-8 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA3026<\/td>\nCaspase-9 Assay Kit (Colorimetric)<\/td>\n<\/tr>\n
                  KTA1020<\/td>\nCell Counting Kit-8 (CCK-8)<\/td>\n<\/tr>\n
                  KTA3030<\/td>\nSenescence \u03b2-Galactosidase Staining Kit<\/td>\n<\/tr>\n
                  KTA1002<\/td>\nLive Cell Tracking Kit (Green Fluorescence)<\/td>\n<\/tr>\n
                  PRP3002<\/td>\nTerminal Deoxynucleotidyl Transferase (TdT)<\/td>\n<\/tr>\n
                  PRP3003<\/td>\nHuman Annexin V\/ANXA5 protein<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

                  \"smart\"<\/a>\"Spectrumlab<\/a><\/p>\n

                  \"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
                  \u7522\u54c1\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
                  \u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

                  \u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

                  \u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

                  \n

                  \u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

                   <\/td>\n

                  		\n

                  TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

                   <\/td>\n

                  		50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
                  \n

                  \u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

                   <\/td>\n

                  		CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTA1020<\/a><\/td>\nCCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
                  \u8ca8\u865fKTA2030<\/a><\/td>\n\n

                  \u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

                  (BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

                  		100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
                  \u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  192\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  colorimetric (540 nm)<\/p>\n

                  \u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  OD 450 nm<\/p>\n

                  \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

                  \u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  2000\u500b\u53cd\u61c9\/<\/p>\n

                  5000\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  OD 562 nm<\/p>\n

                  \u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

                  50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

                  \u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  \u03bbmax= 450 nm<\/p>\n

                  1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

                  human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

                  > 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

                  1ml\u00d75<\/p>\n

                  3mlx5<\/td>\n

                  		More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

                  48\u500b\u53cd\u61c9\/<\/p>\n

                  96\u500b\u53cd\u61c9<\/td>\n

                  		\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  48\u500b\u53cd\u61c9\/<\/p>\n

                  96\u500b\u53cd\u61c9<\/td>\n

                  		Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

                  48\u500b\u53cd\u61c9\/<\/p>\n

                  96\u500b\u53cd\u61c9<\/td>\n

                  		\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  48\u500b\u53cd\u61c9\/<\/p>\n

                  96\u500b\u53cd\u61c9<\/td>\n

                  		500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
                  \u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  48\u500b\u53cd\u61c9\/<\/p>\n

                  96\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  \u00a0\u5438\u6536\u5149505 nm<\/p>\n

                  Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  192\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  \u00a0\u5438\u5149\u503c630nm<\/p>\n

                  4- 300 mg\/dL)<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/strong><\/a>\u00a0\u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  colorimetric (450 nm)<\/p>\n

                  0.03 mM-2 mM<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  593 nm<\/p>\n

                  \u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTE7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\n

                  450 nm<\/p>\n

                  \u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTE0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\n

                  450 nm<\/p>\n

                  \u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTE6032<\/a><\/td>\nTNF<\/em>–\u03b1<\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\n

                  450 nm<\/p>\n

                  7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTE6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\n

                  450 nm<\/p>\n

                  \u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTE7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\n

                  Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

                  \u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  96 X5\u76e4<\/td>\n

                  		\n

                  450 nm<\/p>\n

                  \u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

                  \u8ca8\u865f\u00a0A02051HRP<\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
                  \u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
                  \u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n\n

                  100\u500b\u53cd\u61c9\/<\/p>\n

                  500\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  \uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

                  JC-1 Stain<\/p>\n

                  CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

                  412 nm<\/p>\n

                  2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  50\u500b\u53cd\u61c9\/<\/p>\n

                  200\u500b\u53cd\u61c9<\/td>\n

                  		Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

                  \u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

                   <\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
                  \u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  OD565 nm<\/p>\n

                  96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  OD 450 nm<\/p>\n

                  \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  OD 540 nm<\/p>\n

                  2-75 \u00b5M<\/td>\n<\/tr>\n

                  \u8ca8\u865f<\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

                  OD 664 nm<\/p>\n

                  \u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

                  \u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

                  OD 532 nm<\/p>\n

                  1-100 \u00b5M\/L<\/td>\n<\/tr>\n

                  \u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
                  \u8ca8\u865f<\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

                  96\u500b\u53cd\u61c9\/<\/p>\n

                  480\u500b\u53cd\u61c9<\/td>\n

                  		\n

                  \u00a0OD 565 nm<\/p>\n

                  10,00-100,0000 cells per well<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

                  <\/pre>\n","protected":false},"excerpt":{"rendered":"
                  TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6a59\u8272\u87a2\u5149\uff09 |\u00a0 One-step TUNEL Apoptosis Assay  […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[5751],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42482"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42482"}],"version-history":[{"count":7,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42482\/revisions"}],"predecessor-version":[{"id":50558,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42482\/revisions\/50558"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42482"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42482"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42482"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}