{"id":42467,"date":"2023-05-18T12:47:37","date_gmt":"2023-05-18T04:47:37","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42467"},"modified":"2026-02-04T16:03:27","modified_gmt":"2026-02-04T08:03:27","slug":"%e7%b8%bd%e8%9b%8b%e7%99%bd%e7%a1%ab%e9%86%87%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e6%af%94%e8%89%b2%e6%b3%95%ef%bc%89-total-protein-thiol-assay-kitabcam-%e8%b2%a8%e8%99%9fab219272","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=42467","title":{"rendered":"\u7e3d\u86cb\u767d\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09 | Total Protein Thiol Assay KitAbcam \u8ca8\u865fab219272"},"content":{"rendered":"<p><a href=\"https:\/\/biopioneer.com.tw\/?p=42467&amp;preview=true\"><span style=\"font-size: 12pt; color: #33cccc;\"><strong><span style=\"color: #33cccc;\">\u7e3d\u86cb\u767d\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab219272) | Abcam \u8ca8\u865fab219272<\/span><\/strong><\/span><\/a><\/p>\n<p>Total Protein Thiol Assay Kit (Colorimetric) (ab219272)<\/p>\n<p>\u7e3d\u86cb\u767d\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab219272) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u6e96\u78ba\u7684\u65b9\u6cd5\u4f86\u5b9a\u91cf\u86cb\u767d\u8cea\u4e2d\u7684\u6e38\u96e2\u786b\u9187\u57fa\u5718( free thiol groups )\u3002\u8a72\u6e2c\u5b9a\u4f7f\u7528\u6211\u5011\u5c08\u6709\u7684\u786b\u9187\u50b3\u611f\u5668 (Thiol Blue)\uff0c\u5b83\u5728 OD 680 nm \u8655\u5177\u6709\u6700\u5927\u5438\u5149\u5ea6\u3002\u786b\u9187\u85cd (Thiol Blue)\u8207\u542b\u6709\u6e38\u96e2\u786b\u9187\u57fa\u5718\u7684\u86cb\u767d\u8cea\u6a23\u54c1\u767c\u751f\u53cd\u61c9\u3002\u751f\u6210\u7684\u786b\u9187\u52a0\u5408\u7269\u901a\u904e\u55ae\u500b\u96e2\u5fc3\u67f1\u4ee5\u53bb\u9664\u904e\u91cf\u7684\u786b\u9187\u85cd\u50b3\u611f\u5668\uff0c\u4e26\u6e2c\u91cf\u7d14\u5316\u7522\u7269\u7684\u5438\u6536\u5149\u8b5c\u3002\u786b\u9187(thiol)\u7684\u91cf\u8207\u86cb\u767d\u8cea\u7684\u6bd4\u7387\u662f\u6839\u64da 680 nm \u548c 280 nm \u7684\u5438\u5149\u5ea6\u6bd4\u7387\u8a08\u7b97\u7684\u3002\u8a72\u6e2c\u5b9a\u53ef\u4ee5\u5728\u50b3\u7d71\u7684\u6bd4\u8272\u676f\u3001\u5206\u5149\u5149\u5ea6\u8a08\u6216\u5e36\u6709\u7d2b\u5916\u7dda\u900f\u660e\u677f\u7684\u65b9\u4fbf\u7684 96 \u5b54\u5438\u5149\u5ea6\u677f\u8b80\u53d6\u5668\u4e2d\u9032\u884c\u3002\u86cb\u767d\u8cea\u786b\u9187(thiol)\u5c0d\u86cb\u767d\u8cea\u7d50\u69cb(protein structure)\u3001\u86cb\u767d\u8cea\u529f\u80fd( protein function)\u548c\u751f\u7269\u7cfb\u7d71\u6c27\u5316\u9084\u539f\u74b0\u5883\u975e\u5e38\u91cd\u8981\u5df2\u88ab\u5ee3\u6cdb\u63a5\u53d7\u3002\u4f8b\u5982\uff0c\u767d\u86cb\u767d(albumin)\u662f\u8840\u6f3f\u4e2d\u6700\u8c50\u5bcc\u7684\u86cb\u767d\u8cea\uff0c\u767d\u86cb\u767d\u4e2d\u5b58\u5728\u7684\u6e38\u96e2\u786b\u9187( free thiol )\u88ab\u8a8d\u70ba\u662f\u9ad4\u5167\u4e3b\u8981\u7684\u8840\u6f3f\u6297\u6c27\u5316\u5291\u3002\u767d\u86cb\u767d\u4e2d\u786b\u9187\u72c0\u614b\u7684\u8b8a\u5316\u8207\u8a31\u591a\u75be\u75c5\u548c\u75c5\u75c7\u6709\u95dc\uff0c\u4f8b\u5982\u814e\u81df\u75be\u75c5(kidney disease )\u548c\u5e15\u91d1\u68ee\u75c5( Parkinson\u2019s disease.)\u3002\u5118\u7ba1\u6709\u4e00\u4e9b\u8a66\u5291\u6216\u6aa2\u6e2c\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u5b9a\u91cf\u751f\u7269\u7cfb\u7d71\u4e2d\u7684\u7e3d\u786b\u9187\u542b\u91cf\uff0c\u4f46\u95dc\u9375\u7684\u6311\u6230\u662f\u64c1\u6709\u4e00\u7a2e\u5feb\u901f\u6e96\u78ba\u7684\u65b9\u6cd5\u4f86\u91cf\u5316\u7279\u5b9a\u86cb\u767d\u8cea\u4e2d\u6e38\u96e2\u786b\u9187\u57fa\u5718\u7684\u91cf\u3002<\/p>\n<p><span style=\"color: #800000; font-size: 12pt;\"><strong>\u7e3d\u86cb\u767d\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\u64cd\u4f5c\u6d41\u7a0b\u8aaa\u660e<\/strong><\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/05\/%E7%B8%BD%E8%9B%8B%E7%99%BD%E7%A1%AB%E9%86%87%E6%AA%A2%E6%B8%AC%E8%A9%A6%E5%8A%91%E7%9B%92%EF%BC%88%E6%AF%94%E8%89%B2%E6%B3%95%EF%BC%89ab219272-Abcam-%E8%B2%A8%E8%99%9Fab219272.jpg\" width=\"371\" height=\"277\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/05\/0-\u7e3d\u86cb\u767d\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09ab219272Abcam-\u8ca8\u865fab219272.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-11-300x97.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df 1_\u5de5\u4f5c\u5340\u57df 1\" width=\"149\" height=\"48\" \/><\/a><\/p>\n<table class=\"table skip-auto-productcode-hyperlinking\">\n<thead>\n<tr>\n<th>Components<\/th>\n<th>2 tests<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td title=\"1.5 mL Collecting Tube\">1.5 mL Collecting Tube<\/td>\n<td>2 units<\/td>\n<\/tr>\n<tr>\n<td title=\"2 mL Washing Tube\">2 mL Washing Tube<\/td>\n<td>2 units<\/td>\n<\/tr>\n<tr>\n<td title=\"Assay Buffer\">Assay Buffer<\/td>\n<td>1 x 15ml<\/td>\n<\/tr>\n<tr>\n<td title=\"Spin Column\">Spin Column<\/td>\n<td>2 units<\/td>\n<\/tr>\n<tr>\n<td title=\"Thiol Blue\">Thiol Blue<\/td>\n<td>2 vials<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><img decoding=\"async\" src=\"https:\/\/content.abcam.com\/products\/images\/total-protein-thiol-assay-kit-colorimetric-ab219272--functional-studies-img47756.jpg\/_jcr_content\/renditions\/webp-768.webp\" alt=\"Functional Studies - Total Protein Thiol Assay Kit (Colorimetric) (AB219272)\" \/><\/p>\n<p>Total Protein Thiol Quantification Assay Kit (Colorimetric) (ab219272)<\/p>\n<p>Absorbance (OD) readings obtained using\u00a0BSA as protein sample. BSA absorbacnce at A<sub>280nm\u00a0<\/sub>= 0.699, A<sub>680nm\u00a0<\/sub>= 1.678.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E7%9B%B8%E9%97%9C%E5%84%80%E5%99%A8-300x114.png\" alt=\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e5\u0084\u0080\u00e5\u0099\u00a8\" width=\"97\" height=\"38\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/?p=19075\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%998-300x288.png\" alt=\"CUBEE\u684c\u4e0a\u578b\u5c0f\u578b\u96e2\u5fc3\u6a5f\" width=\"175\" height=\"167\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=18163\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%996-300x288.png\" alt=\"WPACO8000\" width=\"172\" height=\"170\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=6196\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%997-300x288.png\" alt=\"MUPID-2 \u6c34\u5e73\u96fb\u6cf3\u69fd\" width=\"172\" height=\"164\" \/><\/a><a href=\"http:\/\/biopioneer.com.tw\/?p=25739\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%993-300x288.png\" alt=\"BIODROP-\u5be6\u9a57\u8a2d\u50993\" width=\"173\" height=\"168\" \/><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%AF%A6%E9%A9%97%E8%A8%AD%E5%82%991-300x288.png\" alt=\"ELISA reader\" width=\"169\" height=\"160\" \/><\/a><\/p>\n<p><strong>\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7e3d\u86cb\u767d\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09(ab219272) | Abcam \u8ca8\u865fab219272 Total Prot [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":37625,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5261],"tags":[5752],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42467"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42467"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42467\/revisions"}],"predecessor-version":[{"id":54411,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42467\/revisions\/54411"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37625"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42467"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42467"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42467"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}