{"id":42235,"date":"2023-05-05T12:49:48","date_gmt":"2023-05-05T04:49:48","guid":{"rendered":"https:\/\/biopioneer.com.tw\/?p=42235"},"modified":"2023-05-11T18:14:05","modified_gmt":"2023-05-11T10:14:05","slug":"dapi-46-diamidino-2-phenylindole-dihydrochloride-28718-90-3-biotium%e8%b2%a8%e8%99%9f40043-10mg-ml-1ml","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=42235","title":{"rendered":"DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) | 28718-90-3 Biotium\u8ca8\u865f40043 (10mg\/ml, 1ml)\u00a0"},"content":{"rendered":"

DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) | 28718-90-3 Biotium\u8ca8\u865f40043 (10mg\/ml, 1ml)\u00a0<\/a><\/span><\/strong><\/h4>\n

DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochloride) \u662f\u4e00\u7a2e\u6d41\u884c\u7684\u85cd\u8272 DNA \u67d3\u6599\uff0c\u7528\u65bc\u56fa\u5b9a\u7d30\u80de\u7684\u7d93\u5178\u85cd\u8272\u6838\u8907\u67d3\u5291(nuclear counterstain)\u3002\u5982\u87a2\u5149\u986f\u5fae\u93e1( fluorescence microscopy)\u3001\u67d3\u8272\u9ad4\u67d3\u8272(chromosome staining)\u548c\u6d41\u5f0f\u7d30\u80de\u8853( flow cytometry)\u3002\u8a72\u67d3\u6599\u8207 dsDNA \u7684\u5c0f\u6e9d\u7d50\u5408\uff0c\u87a2\u5149\u589e\u5f37\u7d04 20 \u500d\uff0c\u5c0d\u5bcc\u542b A-T \u7684\u5340\u57df\u5177\u6709\u66f4\u9ad8\u7684\u89aa\u548c\u529b\u3002\u4ee5\u7c89\u672b\u6216\u6eb6\u6db2\u5f62\u5f0f\u63d0\u4f9b\uff0c\u53ef\u7528\u65bc\u66f4\u9ad8\u6fc3\u5ea6\u7684\u6d3b\u7d30\u80de\u67d3\u8272\u3002\u03bbEx\/\u03bbEm\uff08\u542b DNA\uff09= 358\/461 nm\u3002\u5132\u5b58\u5728 4\u00b0C \u4e26\u907f\u5149\u3002<\/p>\n

DAPI\u7684\u57fa\u672c\u6a5f\u5236<\/span><\/strong><\/span><\/p>\n

Find the Right Nuclear Stain for Your Application<\/span><\/h3>\n

DAPI \u53ef\u7528\u65bc\u67d3\u8272\u54fa\u4e73\u52d5\u7269\u7d30(mammalian cells)\u4ee5\u53ca\u9769\u862d\u6c0f\u967d\u6027(gram-positive)\u548c\u9769\u862d\u6c0f\u9670\u6027\u7d30\u83cc(gram-negative bacteria)\u3002\u5728\u9175\u6bcd( yeast)\u4e2d\uff0c\u67d3\u8272\u5f88\u5f31\uff0c\u6c92\u6709\u7d30\u80de\u6838\u3002 DAPI \u7684\u819c\u6ef2\u900f\u6027\u4f4e\u65bc Hoechst\uff0c\u901a\u5e38\u7528\u65bc\u56fa\u5b9a\u7d30\u80de\u67d3\u8272\u3002Hoechst \u67d3\u6599\u5177\u6709\u819c\u6ef2\u900f\u6027\uff0c\u66f4\u5e38\u7528\u65bc\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u7d30\u80de\u67d3\u8272\u548c\u7d30\u80de\u9031\u671f\u5206\u6790\u3002\u96d6\u7136 Hoechst \u548c DAPI \u986f\u793a\u51fa\u6bd4\u5d4c\u5165 DNA \u67d3\u6599\u66f4\u4f4e\u7684\u7d30\u80de\u6bd2\u6027\uff0c\u4f46\u5b83\u5011\u5728\u6d3b\u7d30\u80de\u4e2d\u7d50\u5408 DNA \u4e26\u5177\u6709\u6f5b\u5728\u5371\u96aa\u3002 DAPI \u548c Hoechst \u901a\u904e UV \u6fc0\u767c\u9032\u884c\u5149\u8f49\u63db\u4ee5\u5f62\u6210\u7da0\u8272\u87a2\u5149\u67d3\u6599\uff0c\u9019\u6703\u5c0e\u81f4\u591a\u8272\u6210\u50cf\u4e2d\u7684\u507d\u5f71\u3002\u6709\u95dc\u8a73\u7d30\u4fe1\u606f\uff0c\u8acb\u53c3\u95b1\u6211\u5011\u7684\u6280\u8853\u63d0\u793a\u907f\u514d DAPI \u548c Hoechst \u7684 UV \u5149\u8f49\u63db\u7522\u751f\u7684\u507d\u5f71\u3002<\/p>\n

DAPI \u4e0d\u80fd\u6ef2\u900f\u6d3b\u7d30\u80de\uff0c\u4f46\u5f88\u6709\u7528\u4f5c\u70ba\u56fa\u5b9a\u7d30\u80de\u6216\u7d44\u7e54\u5207\u7247\u7684\u6838\u8907\u67d3\u5291\u3002\u5728\u66f4\u9ad8\u7684\u6fc3\u5ea6(~10 ug\/mL)\uff0cDAPI \u53ef\u7528\u65bc\u6d3b\u7d30\u80de\u67d3\u8272\u3002<\/p>\n\n\n\n\n\n\n\n
Product<\/th>\nCatalog Number<\/th>\nMW<\/th>\nUnit Size<\/th>\nFormat<\/th>\n<\/tr>\n<\/thead>\n
DAPI Powder
\n(dilactate)<\/td>\n		40009<\/td>\n457.49<\/td>\n10 mg<\/td>\nYellow solid<\/td>\n<\/tr>\n
DAPI Powder
\n(dihydrochloride)<\/td>\n		40011<\/td>\n350.25<\/td>\n10 mg<\/td>\nYellow solid<\/td>\n<\/tr>\n
DAPI Solution
\n(dilactate)<\/td>\n		40043<\/td>\n457.49<\/td>\n1 mL
\n(10 mg\/mL in water)<\/td>\n		Yellow solution<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
CAS number<\/th>\n28718-90-3<\/td>\n<\/tr>\n
Probe cellular localization<\/th>\nNucleus<\/td>\n<\/tr>\n
For live or fixed cells<\/th>\nFor fixed cells, For live\/intact cells<\/td>\n<\/tr>\n
Detection method\/readout<\/th>\nFluorescence microscopy, Flow cytometry<\/td>\n<\/tr>\n
Assay type\/options<\/th>\nDNA content\/cell cycle profiling by flow cytometry, Long term staining (24-72h), No-wash staining, Real-time imaging, Tissue staining<\/td>\n<\/tr>\n
Cell permeability<\/th>\nMembrane permeant<\/td>\n<\/tr>\n
Apoptosis\/viability marker<\/th>\nAll cell stain<\/td>\n<\/tr>\n
Colors<\/th>\nBlue<\/td>\n<\/tr>\n
Excitation\/Emission<\/th>\n358\/461 nm (with DNA)<\/td>\n<\/tr>\n
Storage Conditions<\/th>\nStore at 2 to 8 \u00b0C, Protect from light<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
<\/div>\n
DAPI\u67d3\u8272-\u7d30\u80de\/\u7d44\u7e54\/\u7d30\u83cc\u6216\u9175\u6bcd<\/strong><\/span><\/div>\n
DAPI\u6d3b\u7d30\u80de\u67d3\u8272(Live cell staining)<\/span><\/strong><\/span><\/div>\n
Below we provide two protocols for staining live cells with DAPI. Staining by medium exchange results in uniform exposure of cells to probe. However, for some cell types, morphology or viability may be affected by medium exchange. In addition, floating dead cells may be lost during medium removal, and suspension cells must be collected by centrifugation to exchange the medium. Direct addition of 10X probe is a convenient staining method that doesn\u2019t require medium exchange, but care must be taken to mix immediately yet gently to avoid high transient probe concentration or disruption of cells by pipetting. Note that we do not recommend adding highly concentrated dye directly to cells in culture, as this will result in local areas of high dye exposure.<\/div>\n
<\/div>\n
DAPI \u901a\u904e\u57f9\u990a\u57fa\u4ea4\u63db\u9032\u884c\u6d3b\u7d30\u80de\u67d3\u8272(Live cell staining by medium exchange)\u00a0<\/span><\/strong><\/span><\/div>\n
1. Dilute DAPI to 10 ug\/mL in fresh, complete culture medium. DAPI can be
\ncombined with other fluorescent probes.
\n2. Remove medium from the cells and replace with medium containing dye.
\n3. Incubate cells at room temperature or 37\u00b0C for 5-15 minutes, then image.
\nNote: Washing is not necessary for specific staining, but nuclear staining is
\nstable after washing.<\/div>\n
Live cell staining by direct addition of 10X probe
\n1. Prepare 10X dye solution by diluting DAPI to 100 ug\/mL in fresh, complete
\nculture medium. DAPI can be combined with other fluorescent probes, which
\nshould be diluted to 10 times the final desired concentration.
\n2. Without removing the medium from the cells, add 1\/10 volume of 10X dye
\ndirectly to the well.
\n3. Immediate mix thoroughly by gently pipetting the medium up and down.
\nFor larger well sizes (e.g., 24-well to 6-well plates), the plate can be gently
\nswirled to mix.
\n4. Incubate cells at room temperature or 37\u00b0C for 5-15 minutes, then image.
\nNote: Washing is not necessary for specific staining, but nuclear staining is
\nstable after washing.<\/div>\n
<\/div>\n
DAPI \u56fa\u5b9a\u7d30\u80de\u6216\u7d44\u7e54\u5207\u7247\u7684\u67d3\u8272 (Staining of fixed cells or tissue sections)\u00a0<\/span><\/strong><\/span><\/div>\n
1. Dilute DAPI to 1 ug\/mL in PBS. DAPI can be included together with
\nantibodies or other probes, and can be diluted in buffers with detergent or
\nblocking agents if convenient.
\n2. Add the PBS with dye to cells or tissue sections and incubate at room
\ntemperature for at least 5 minutes.
\n3. Image the samples; washing is optional but not required.
\nNote: Samples can be stored at 4\u00b0C after staining and before imaging.
\nNote: DAPI can be included directly in antifade mounting medium for onestep mounting and staining. When using DAPI in mounting medium, longer
\nincubation times may be required for DAPI to completely penetrate the cell
\nnuclei.<\/div>\n
<\/div>\n
DAPI \u67d3\u8272\u7d30\u83cc\u6216\u9175\u6bcd (Staining bacteria or yeast)\u00a0<\/span><\/strong><\/div>\n
DAPI stains bacteria more dimly than mammalian cells. Live or killed bacteria can
\nbe stained with 10 ug\/mL DAPI in PBS or 150 mM NaCl for 30 minutes at room
\ntemperature. DAPI tends to stain dead cells more brightly than live cells.
\nIn S. cerevisiae, DAPI preferentially stains dead yeast with nuclear and
\ncytoplasmic staining when used at 10 ug\/mL in PBS; in live yeast DAPI shows dim
\nmitochondrial staining.<\/div>\n
\n

\"biotium\"<\/p>\n

Biotium \u63d0\u4f9b\u7bc4\u570d\u5ee3\u6cdb\u7684\u87a2\u5149\u63a2\u91dd\u7528\u65bc\u7814\u7a76\u51cb\u4ea1\u7d30\u80de(Apotosis)<\/span><\/strong><\/p>\n

\"\"<\/p>\n

The\u00a0NucView\u00ae Caspase-3 Substrate<\/a>\u00a0comes in 3 colors, and measures caspase activity in real time, in live cells.\u00a0MitoView\u2122 633<\/a>\u00a0can measure the decrease in mitochondria membrane potential that occurs in early apoptosis.\u00a0CF\u00ae Dye Annexin V<\/a>\u00a0conjugates come in many color options, and label PS on the membranes of late apoptotic cells. We also offer kits containing combinations of these probes.<\/p>\n

 <\/p>\n<\/div>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

DAPI (4′,6-Diamidino-2-Phenylindole, dihydrochlor […]<\/p>\n","protected":false},"author":3,"featured_media":40136,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5378],"tags":[5718,5716,5715,5717],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42235"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=42235"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42235\/revisions"}],"predecessor-version":[{"id":42239,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/42235\/revisions\/42239"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/40136"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=42235"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=42235"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=42235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}