{"id":41788,"date":"2023-04-26T18:27:27","date_gmt":"2023-04-26T10:27:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=41788"},"modified":"2025-09-03T11:39:38","modified_gmt":"2025-09-03T03:39:38","slug":"mtt-thiazolyl-blue-cas-298-93-1-mce%e8%b2%a8%e8%99%9fhy-15924","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=41788","title":{"rendered":"MTT (Thiazolyl Blue) | Cas #298-93-1 MCE\u8ca8\u865fHY-15924"},"content":{"rendered":"

MCE (MedChem Express)<\/span> -MTT (Thiazolyl Blue)<\/span><\/span><\/span><\/strong><\/span><\/p>\n

MCE\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u65b0\u6fa4\u897f\uff0c\u63d0\u4f9b\u7a2e\u985e\u9f4a\u5168\u7684\u5316\u5b78\u53ca\u751f\u5316\u7814\u7a76\u7528\u7522\u54c1\uff0c\u5305\u62ec\u5275\u65b0\u7684\u751f\u547d\u79d1\u5b78\u8a66\u5291\uff0creference compounds(\u6a19\u6e96\u5316\u5408\u7269)\uff0cAPIs(\u539f\u6599\u85e5)\u53caNatural compounds(\u5929\u7136\u5316\u5408\u7269)\u4ee5\u63d0\u4f9b\u5be6\u9a57\u5ba4\u6216\u79d1\u5b78\u7814\u7a76\u4e4b\u7528\uff0cMedChemExpress(MCE)\u7522\u54c150000+ \u7279\u7570\u6027\u6291\u5236\u5291\u3001\u5316\u5408\u7269\u5eab\u3001\u91cd\u7d44\u86cb\u767d\uff0c\u5c08\u6ce8\u65bc\u4fe1\u865f\u901a\u8def\u548c\u75be\u75c5\u7814\u7a76\u9818\u57df\u3002\u5168\u7403\u6587\u737b\u5ee3\u6cdb\u5f15\u7528\uff0c\u8cea\u63a7\u9ad4\u7cfb\u56b4\u683c\uff0c\u54c1\u8cea100%\u3002<\/p>\n

<\/p>\n

MTT\u00a0(Thiazolyl Blue) | Cas #298-93-1 MCE\u8ca8\u865fHY-15924\u00a0<\/a><\/span><\/h4>\n

Thiazolyl Blue (Synonyms: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide)<\/span><\/p>\n

\"\"<\/p>\n

\n

For research use only. We do not sell to patients.<\/b><\/p>\n<\/div>\n

\n
\n

Thiazolyl Blue<\/em>\u00a0Chemical Structure<\/p>\n

CAS No. :\u00a0298-93-1<\/a><\/p>\n<\/div>\n<\/div>\n

\u567b\u5511\u85cd (Thiazolyl Blue, MTT) \u662f\u4e00\u7a2e\u53ef\u6ef2\u900f\u7d30\u80de\u4e14\u5e36\u6b63\u96fb\u8377\u7684\u56db\u5511\u67d3\u6599(\u00a0tetrazolium dye )\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u4e2d\u7684\u9084\u539f\u4ee3\u8b1d(reductive metabolism)\u3002\u567b\u5511\u85cd(\u00a0Thiazolyl Blue )\u901a\u904e\u8cea\u819c(plasma membrane )\u88ab\u7d30\u80de\u5438\u6536\uff0c\u7136\u5f8c\u88ab\u7d30\u80de\u5167 NAD (P) H-\u6c27\u5316\u9084\u539f\u9176(NAD (P) H-oxidoreductases)\u9084\u539f\u70baformazan\u3002\u567b\u5511\u85cd(\u00a0Thiazolyl Blue )\u7d93\u5e38\u7528\u65bc\u6bd4\u8272\u6e2c\u5b9a\u4e2d\u4ee5\u6e2c\u91cf\u7d30\u80de\u589e\u6b96(cell proliferation)\u3001\u7d30\u80de\u6bd2\u6027(cytotoxicity)\u548c\u7d30\u80de\u51cb\u4ea1(apoptosis)\u3002<\/p>\n

Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and\u00a0apoptosis<\/a>.\u00a0\u00a0Storage: protect from light.<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n
Molecular Weight<\/th>\n\n
\n

414.32<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Appearance<\/th>\n\n
\n

Solid<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Formula<\/th>\n\n
\n

C18<\/sub>H16<\/sub>BrN5<\/sub>S<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

CAS No.<\/th>\n\n
SMILES<\/th>\n\n
\n

CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Shipping<\/th>\n\n
\n

Room temperature in continental US; may vary elsewhere.<\/p>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\"<\/span><\/strong><\/span><\/p>\n

MTT\u5de5\u4f5c\u6db2\u7684\u914d\u88fd (\u6bcf\u7a2e\u7d30\u80de\u689d\u4ef6\u4e0d\u540c)\u00a0<\/span><\/strong><\/span><\/p>\n

\u7528 PBS \u6eb6\u89e3 MTT \u4ee5\u7372\u5f97 5 mg\/mL \u7684 MTT\u3002
\n\u7d30\u80de\u589e\u6b96\u6aa2\u6e2c\uff0896\u5b54\u677f\uff09
\n1. \u63a5\u7a2e\u7d30\u80de\uff1a\u7528\u542b10%FBS\u7684\u57f9\u990a\u57fa\u88fd\u5099\u55ae\u7d30\u80de\u61f8\u6db2\uff0c\u63a5\u7a2e\u65bc96\u5b54\u677f\u4e2d\uff0c\u6bcf\u5b541000-10000\u500b\u7d30\u80de\uff0c\u6bcf\u5b54\u9ad4\u7a4d100uL\u3002
\n2. \u57f9\u990a\u7d30\u80de\uff1a37\u2103\u3002 5% CO2\uff0c\u5b75\u80b2 24-72 \u5c0f\u6642\u3002
\n3. \u6bcf\u5b54\u52a0\u516510 \u03bcL MTT\uff0c\u5b75\u80b24 h\uff0c\u68c4\u4e0a\u6e05\uff0c\u61f8\u6d6e\u7d30\u80de\u9700\u5148\u96e2\u5fc3\u3002
\n4. \u52a0\u5165100 \u03bcL DMSO\uff0c\u9707\u76ea10 min\u4f7f\u6676\u9ad4\u5b8c\u5168\u6eb6\u89e3
\n5. \u7528\u5438\u5149\u5ea6\u677f\u8b80\u6578\u5668\u5728 OD = 562 nm \u8655\u76e3\u6e2c\u5438\u5149\u5ea6\u589e\u52a0\u3002<\/p>\n

\n

Preparation:<\/span><\/strong><\/span>
\nIt is soluble in water (10 mg\/ml), ethanol (20 mg\/ml) and 2-methoxyethanol (20mg\/ml).
\nIt is also soluble in buffered salt solutions and culture media (5 mg\/ml).
\nStorage\/Stability:<\/span><\/strong><\/span>
\nMTT is light sensitive.
\nReconstituted MTT solution is stable for at least 6 months when stored frozen (-20 \u00b0C).
\nStorage of a reconstituted MTT solution at 2 – 8 \u00b0C for more than 2 weeks may cause decomposition and yield erroneous results.<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

MedChemexpress (MCE) \u7522\u54c1<\/span><\/h4>\n

MCE (MedChemexpress) \u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u65b0\u6fa4\u897f\uff0c\u63d0\u4f9b\u7a2e\u985e\u9f4a\u5168\u7684\u5316\u5b78\u53ca\u751f\u5316\u7814\u7a76\u7528\u7522\u54c1\uff0c\u5305\u62ec\u5275\u65b0\u7684\u751f\u547d\u79d1\u5b78\u8a66\u5291\uff0creference compounds (\u6a19\u6e96\u5316\u5408\u7269)\uff0cAPIs (\u539f\u6599\u85e5) \u53caNatural compounds (\u5929\u7136\u5316\u5408\u7269)\u4ee5\u63d0\u4f9b\u5be6\u9a57\u5ba4\u6216\u79d1\u5b78\u7814\u7a76\u4e4b\u7528\uff0cMedChemExpress(MCE)\u7522\u54c150000+ \u7279\u7570\u6027\u6291\u5236\u5291\u3001\u5316\u5408\u7269\u5eab\u3001\u91cd\u7d44\u86cb\u767d\uff0c\u5c08\u6ce8\u65bc\u4fe1\u865f\u901a\u8def\u548c\u75be\u75c5\u7814\u7a76\u9818\u57df\u3002\u5168\u7403\u6587\u737b\u5ee3\u6cdb\u5f15\u7528\uff0c\u8cea\u63a7\u9ad4\u7cfb\u56b4\u683c\uff0c\u54c1\u8cea100%\u3002\u5b98\u7db2\u9023\u7d50<\/span> https:\/\/www.medchemexpress.com<\/a><\/span><\/span><\/p>\n

\"\"<\/p>\n

MedChemexpress (MCE) \u7522\u54c1\u985e\u5225\uff0d\u4f9d\u7814\u7a76\u985e\u578b\u5340\u5206\uff1a<\/span><\/h4>\n

\u2022Anti-infection<\/a><\/span><\/strong><\/h4>\n

\u2022Antibody-drug Conjugate\/ADC Related<\/a><\/span><\/strong><\/h4>\n

\u2022Apoptosis<\/a><\/span><\/strong><\/h4>\n

\u2022Autophagy<\/a><\/span><\/strong><\/h4>\n

\u2022Cell Cycle\/DNA Damage<\/a><\/span><\/strong><\/h4>\n

\u2022Cytoskeleton<\/a><\/span><\/strong><\/h4>\n

\u2022Epigenetics<\/a><\/span><\/strong><\/h4>\n

\u2022GPCR\/G Protein<\/a><\/span><\/strong><\/h4>\n

\u2022Immunology\/Inflammation<\/a><\/span><\/strong><\/h4>\n

\u2022JAK\/STAT Signaling<\/a>\u2022MAPK\/ERK Pathway<\/a><\/span><\/strong><\/h4>\n

\u2022Membrane Transporter\/Ion Channel<\/a><\/span><\/strong><\/h4>\n

\u2022Metabolic Enzyme\/Protease<\/a><\/span><\/strong><\/h4>\n

\u2022Neuronal Signaling<\/a><\/span><\/strong><\/h4>\n

\u2022NF-\u03baB<\/a><\/span><\/strong><\/h4>\n

\u2022PI3K\/Akt\/mTOR<\/a><\/span><\/strong><\/h4>\n

\u2022PROTAC<\/a><\/span><\/strong><\/h4>\n

\u2022Protein Tyrosine Kinase\/RTK<\/a>\u2022Stem Cell\/Wnt<\/a><\/span><\/strong><\/h4>\n

\u2022TGF-beta\/Smad<\/a><\/span><\/strong><\/h4>\n

\u2022Vitamin D Related<\/a><\/span><\/strong><\/h4>\n

\u2022Others<\/a><\/span><\/strong><\/h4>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

MCE (MedChem Express) -MTT (Thiazolyl Blue) MCE\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u65b0\u6fa4 […]<\/p>\n","protected":false},"author":3,"featured_media":52857,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5369],"tags":[6867,5629,6866,6869,6865,6868],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41788"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=41788"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41788\/revisions"}],"predecessor-version":[{"id":52859,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/41788\/revisions\/52859"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/52857"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=41788"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=41788"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=41788"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}