{"id":40907,"date":"2023-04-10T16:01:48","date_gmt":"2023-04-10T08:01:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40907"},"modified":"2025-09-03T11:55:21","modified_gmt":"2025-09-03T03:55:21","slug":"h2dcfda-%e6%b4%bb%e6%80%a7%e6%b0%a7-ros-%e8%9e%a2%e5%85%89%e6%8e%a2%e9%87%9d-cas4091-99-0-mce%e8%b2%a8%e8%99%9fhy-d0940","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=40907","title":{"rendered":"H2DCFDA | \u6d3b\u6027\u6c27 (ROS) \u87a2\u5149\u63a2\u91dd CAS#4091-99-0 MCE\u8ca8\u865fHY-D0940"},"content":{"rendered":"

MCE (MedChem Express)<\/span> -\u6d3b\u6027\u6c27 (ROS) \u87a2\u5149\u63a2\u91dd (H2DCFDA)
\n<\/span><\/span><\/strong><\/span><\/p>\n

MCE\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u65b0\u6fa4\u897f\uff0c\u63d0\u4f9b\u7a2e\u985e\u9f4a\u5168\u7684\u5316\u5b78\u53ca\u751f\u5316\u7814\u7a76\u7528\u7522\u54c1\uff0c\u5305\u62ec\u5275\u65b0\u7684\u751f\u547d\u79d1\u5b78\u8a66\u5291\uff0creference compounds (\u6a19\u6e96\u5316\u5408\u7269)\uff0cAPIs (\u539f\u6599\u85e5) \u53caNatural compounds (\u5929\u7136\u5316\u5408\u7269)\u4ee5\u63d0\u4f9b\u5be6\u9a57\u5ba4\u6216\u79d1\u5b78\u7814\u7a76\u4e4b\u7528\uff0cMedChemExpress(MCE)\u7522\u54c150000+ \u7279\u7570\u6027\u6291\u5236\u5291\u3001\u5316\u5408\u7269\u5eab\u3001\u91cd\u7d44\u86cb\u767d\uff0c\u5c08\u6ce8\u65bc\u4fe1\u865f\u901a\u8def\u548c\u75be\u75c5\u7814\u7a76\u9818\u57df\u3002\u5168\u7403\u6587\u737b\u5ee3\u6cdb\u5f15\u7528\uff0c\u8cea\u63a7\u9ad4\u7cfb\u56b4\u683c\uff0c\u54c1\u8cea100%\u3002<\/p>\n

<\/p>\n

H2DCFDA | \u6d3b\u6027\u6c27 (ROS) \u87a2\u5149\u63a2\u91dd CAS#4091-99-0 MCE\u8ca8\u865fHY-D0940<\/a><\/span><\/h4>\n

(Synonyms: DCFH-DA; 2′,7′-Dichlorodihydrofluorescein diacetate)<\/p>\n

\"H2DCFDA<\/p>\n

H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species<\/span><\/h5>\n

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular\u00a0reactive oxygen species<\/a>\u00a0(ROS)<\/b>\u00a0(Ex\/Em=488\/525 nm). H2DCFDA (DCFH-DA) \u662f\u4e00\u7a2e\u7d30\u80de\u6ef2\u900f\u6027\u63a2\u91dd\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u5167\u6d3b\u6027\u6c27 (ROS) (Ex\/Em=488\/525 nm)\u3002<\/p>\n

\"\"<\/p>\n

 <\/p>\n

H2DCFDA<\/strong>\u00a0purchased from MCE. Usage Cited in:\u00a0Curr Biol<\/cite>. 2021 May 18;S0960-9822(21)00607-2. \u00a0<\/a>[Abstract]<\/b><\/a><\/span><\/p>\n

Co-staining stigmas with H2DCFDA (50 \u03bcM; for 1-2 hours) and the cell wall indicator propidium iodide (PI) shows that ROS is located in the cytoplasm of papilla cells, near the periphery of the plasma membrane.<\/p>\n

 <\/p>\n

The staining procedure is the same as for the H2DCFDA[1].\uff08\u4ee5\u4e0b\u662f\u6211\u5011\u63a8\u85a6\u7684\u65b9\u6848\uff0c\u672c\u65b9\u6848\u50c5\u63d0\u4f9b\u6307\u5357\uff0c\u61c9\u6839\u64da\u60a8\u7684\u5177\u9ad4\u9700\u8981\u9032\u884c\u4fee\u6539\uff09\u3002
\n1. H2DCFDA \u6eb6\u65bc DMSO \u5f97\u5230 10 mM \u5132\u5099\u6db2\uff0c\u4f7f\u7528\u524d\u9032\u4e00\u6b65\u7a00\u91cb\u3002
\n2. \u7d30\u80de\u8207 5 \u00b5M H2DCFDA \u6eb6\u6db2\u5728 PBS \u4e2d\u65bc 37\u00b0C \u907f\u5149\u5b75\u80b2 30 \u5206\u9418\uff0c\u7136\u5f8c\u7528 0.05% \u80f0\u86cb\u767d\u9176-EDTA \u6eb6\u6db2\u6536\u96c6\u7d30\u80de\uff0c\u61f8\u6d6e\u5728\u65b0\u9bae\u57f9\u990a\u57fa\u4e2d\uff0c\u7acb\u5373\u7528\u6d41\u5f0f\u7d30\u80de\u5100\u5206\u6790\u3002
\n3. \u5982\u679c\u9700\u8981\uff0c\u8207 H2DCFDA \u63a2\u91dd\u4e00\u8d77\u4f7f\u7528\u87a2\u5149\u7d20\u67d3\u6599 DCFDA \u7684 ROS \u4e0d\u654f\u611f\u4fee\u98fe\u4f5c\u70ba\u967d\u6027\u5c0d\u7167\u3002\u67d3\u8272\u7a0b\u5e8f\u8207 H2DCFDA [1] \u76f8\u540c\u3002<\/p>\n

\"\"<\/p>\n

 <\/p>\n

H2DCFDA<\/strong>\u00a0purchased from MCE. Usage Cited in:\u00a0Biomaterials<\/cite>. 2022 Jul 21;287:121688. \u00a0<\/a>[Abstract]<\/b><\/a><\/span><\/p>\n

CLSM images of 4T1 tumor cells stained with DCFH-DA (15 min) after different treatments.<\/p>\n

 <\/p>\n

\"\"<\/p>\n

 <\/p>\n

H2DCFDA<\/strong>\u00a0purchased from MCE. Usage Cited in:\u00a0ACS Nano<\/cite>. 2021 Oct 26;15(10):16286-16297. \u00a0<\/a>[Abstract]<\/b><\/a>\u00a0<\/span><\/p>\n

CLSM image of 4T1 cells after being treated with MM, MMP, and MMTP; cells are stained with DCFH-DA (20 \u03bcM; for 1 h).<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

<\/p>\n

<\/p>\n

\"\"<\/p>\n

MedChemexpress (MCE) \u7522\u54c1<\/span><\/h4>\n

MCE (MedChemexpress) \u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u65b0\u6fa4\u897f\uff0c\u63d0\u4f9b\u7a2e\u985e\u9f4a\u5168\u7684\u5316\u5b78\u53ca\u751f\u5316\u7814\u7a76\u7528\u7522\u54c1\uff0c\u5305\u62ec\u5275\u65b0\u7684\u751f\u547d\u79d1\u5b78\u8a66\u5291\uff0creference compounds (\u6a19\u6e96\u5316\u5408\u7269)\uff0cAPIs (\u539f\u6599\u85e5) \u53caNatural compounds (\u5929\u7136\u5316\u5408\u7269)\u4ee5\u63d0\u4f9b\u5be6\u9a57\u5ba4\u6216\u79d1\u5b78\u7814\u7a76\u4e4b\u7528\uff0cMedChemExpress(MCE)\u7522\u54c150000+ \u7279\u7570\u6027\u6291\u5236\u5291\u3001\u5316\u5408\u7269\u5eab\u3001\u91cd\u7d44\u86cb\u767d\uff0c\u5c08\u6ce8\u65bc\u4fe1\u865f\u901a\u8def\u548c\u75be\u75c5\u7814\u7a76\u9818\u57df\u3002\u5168\u7403\u6587\u737b\u5ee3\u6cdb\u5f15\u7528\uff0c\u8cea\u63a7\u9ad4\u7cfb\u56b4\u683c\uff0c\u54c1\u8cea100%\u3002\u5b98\u7db2\u9023\u7d50<\/span> https:\/\/www.medchemexpress.com<\/a><\/span><\/span><\/p>\n

\u2022Anti-infection<\/a><\/span><\/strong><\/h4>\n

\u2022Antibody-drug Conjugate\/ADC Related<\/a><\/span><\/strong><\/h4>\n

\u2022Apoptosis<\/a><\/span><\/strong><\/h4>\n

\u2022Autophagy<\/a><\/span><\/strong><\/h4>\n

\u2022Cell Cycle\/DNA Damage<\/a><\/span><\/strong><\/h4>\n

\u2022Cytoskeleton<\/a><\/span><\/strong><\/h4>\n

\u2022Epigenetics<\/a><\/span><\/strong><\/h4>\n

\u2022GPCR\/G Protein<\/a><\/span><\/strong><\/h4>\n

\u2022Immunology\/Inflammation<\/a><\/span><\/strong><\/h4>\n

\u2022JAK\/STAT Signaling<\/a>\u2022MAPK\/ERK Pathway<\/a><\/span><\/strong><\/h4>\n

\u2022Membrane Transporter\/Ion Channel<\/a><\/span><\/strong><\/h4>\n

\u2022Metabolic Enzyme\/Protease<\/a><\/span><\/strong><\/h4>\n

\u2022Neuronal Signaling<\/a><\/span><\/strong><\/h4>\n

\u2022NF-\u03baB<\/a><\/span><\/strong><\/h4>\n

\u2022PI3K\/Akt\/mTOR<\/a><\/span><\/strong><\/h4>\n

\u2022PROTAC<\/a><\/span><\/strong><\/h4>\n

\u2022Protein Tyrosine Kinase\/RTK<\/a>\u2022Stem Cell\/Wnt<\/a><\/span><\/strong><\/h4>\n

\u2022TGF-beta\/Smad<\/a><\/span><\/strong><\/h4>\n

\u2022Vitamin D Related<\/a><\/span><\/strong><\/h4>\n

\u2022Others<\/a><\/span><\/strong><\/h4>\n","protected":false},"excerpt":{"rendered":"

MCE (MedChem Express) -\u6d3b\u6027\u6c27 (ROS) \u87a2\u5149\u63a2\u91dd (H2DCFDA) MCE\u7e3d\u90e8\u4f4d\u65bc […]<\/p>\n","protected":false},"author":3,"featured_media":52872,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5369],"tags":[5547],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40907"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40907"}],"version-history":[{"count":6,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40907\/revisions"}],"predecessor-version":[{"id":40910,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40907\/revisions\/40910"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/52872"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40907"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40907"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40907"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}