{"id":40871,"date":"2023-04-08T14:02:45","date_gmt":"2023-04-08T06:02:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40871"},"modified":"2025-09-03T12:00:21","modified_gmt":"2025-09-03T04:00:21","slug":"%e8%87%aa%e5%99%ac%e6%b3%a1autophagic-vacuoles%e8%9e%a2%e5%85%89%e6%a8%99%e8%aa%8c-mce%e8%b2%a8%e8%99%9fhy-d1027","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=40871","title":{"rendered":"\u81ea\u566c\u6ce1(autophagic vacuoles)\u87a2\u5149\u6a19\u8a8c-MDC\u67d3\u5291 | CAS#10121-91-2 MCE\u8ca8\u865fHY-D1027"},"content":{"rendered":"

\u81ea\u566c\u6ce1(autophagic vacuoles)\u87a2\u5149\u6a19\u8a8c | CAS#10121-91-2MCE\u8ca8\u865fHY-D1027\u00a0<\/a><\/span><\/h4>\n

A conventional fluorescent probe, monodansylcadaverine (MDC), has served as a useful fluorescent marker for lysosomal\/ autophagic vacuoles. However, it is known to generate high background and weak fluorescent signal.<\/p>\n

<\/p>\n

\u81ea\u566c(Autophagy )<\/span><\/p>\n

\u81ea\u566c(Autophagy )\u662f\u4e00\u7a2e\u7d30\u80de\u5167\u964d\u89e3\u7cfb\u7d71(intracellular degradation system )\uff0c\u53ef\u5c07\u7d30\u80de\u8cea\u6210\u5206\u8f38\u9001\u5230\u6eb6\u9176\u9ad4(lysosome)\u3002\u81ea\u566c\u4f5c\u7528(Autophagy)\u5ee3\u6cdb\u591a\u7a2e\u751f\u7406(physiologica)\u548c\u75c5\u7406\u751f\u7406(pathophysiological)\u4f5c\u7528\u3002\u4e0d\u540c\u7684\u9078\u64c7\u6027\u81ea\u566c\u5f62\u5f0f(\u00a0autophagy)\u5c0e\u81f4\u7d30\u80de\u5668\u6216\u75c5\u539f\u9ad4\u7684\u7279\u7570\u6027\u964d\u89e3\u3002\u9019\u4e9b\u9078\u64c7\u6027\u9014\u5f91\u5305\u62ec\u81ea\u566c\u7dda\u7c92\u9ad4 (mitophagy)\u3001\u904e\u6c27\u5316\u7269\u9176\u9ad4 (pexophagy)\u3001\u5167\u8cea\u7db2 (reticulophagy \u6216 ER-phagy) \u7684\u964d\u89e3\uff0c\u6838\u91a3\u9ad4 (ribophagy)\u3001\u86cb\u767d\u8cea\u805a\u96c6\u9ad4 (aggrephagy)\u3001\u8102\u6ef4 (lipophagy)\u3001\u80f0\u817a\u7d30\u80de\u5167\u7684\u5206\u6ccc\u9846\u7c92\uff08zymophagy\uff09\u6216\u7d30\u80de\u5167\u75c5\u539f\u9ad4\uff08\u7570\u9ad4\u81ea\u566c\uff09\u3002\u81ea\u566c\u7531\u5e7e\u500b\u9023\u7e8c\u7684\u6b65\u9a5f\u7d44\u6210: \u9694\u96e2(sequestration)\u3001\u904b\u8f38\u5230\u6eb6\u9176\u9ad4(\u00a0lysosomes)\u3001\u964d\u89e3(degradation)\u548c\u964d\u89e3\u7522\u7269\u5229\u7528(utilization of degradation products)\uff0c\u6bcf\u4e00\u6b65\u90fd\u53ef\u80fd\u767c\u63ee\u4e0d\u540c\u7684\u4f5c\u7528\u3002\u81ea\u566c\u4fe1\u865f\u8f49\u5c0e(Autophagy signal transduction)\u4e3b\u8981\u53d7\u81ea\u566c\u76f8\u95dc\u8abf\u63a7\u57fa\u56e0\/\u86cb\u767d\u8cea\uff0cAtgs\u3002 ATGs \u63ed\u793a\u4e86\u81ea\u566c\u9ad4\u5f62\u6210\u7684\u5927\u90e8\u5206\u6a5f\u5236\u3002\u6b64\u5916\uff0c\u4e0d\u540c\u7684\u975e ATG\u86cb\u767d\u8cea\u53c3\u8207\u81ea\u566c\u7684\u8abf\u7bc0\u548c\u904e\u7a0b\uff0c\u4f8b\u5982 mTOR\u3001AMPK\u3001AKT\u3001AMBRA1\u3001BCL2\u3001DFCP1 \u6216 VPS34\u3002\u81ea\u566c(Autophagy )\u53ca\u5176\u5931\u8abf\u8207\u4e0d\u540c\u7684\u4eba\u985e\u75be\u75c5\u6216\u904e\u7a0b\u6709\u95dc\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u795e\u7d93\u8b8a\u6027(neurodegeneration)\u3001\u514d\u75ab\u529b(immunity)\u6216\u8870\u8001(aging)\u3002\u8a31\u591a\u85e5\u7269\u548c\u5929\u7136\u7522\u7269\u90fd\u53c3\u8207\u81ea\u566c\u8abf\u7bc0(autophagy modulation)\uff0c\u7121\u8ad6\u662f\u901a\u904e\u591a\u7a2e\u4fe1\u865f\u901a\u8def\u8a98\u5c0e\u6216\u6291\u5236\u7d30\u80de\u81ea\u566c\u3002\u6b64\u5916\uff0c\u4e00\u4e9b\u6cbb\u7642\u7cd6\u5c3f\u75c5\u7684\u81e8\u5e8a\u85e5\u7269\u548c\u5316\u5408\u7269\u4e5f\u88ab\u767c\u73fe\u6d89\u53ca\u81ea\u566c\u8abf\u7bc0\u3002<\/p>\n

Dansylcadaverine (Synonyms: Monodansyl cadaverine)<\/span><\/p>\n

Dansylcadaverine (Monodansyl cadaverine, MDC) \u662f\u4e00\u7a2e\u81ea\u767c\u87a2\u5149\u5316\u5408\u7269(autofluorescent compound )\uff0c\u7528\u65bc\u6a19\u8a18\u81ea\u566c\u6ce1(autophagic vacuoles)\u3002 Dansylcadaverine (Monodansyl cadaverine, MDC)\u00a0 \u662f\u8f49\u8c37\u6c28\u91af\u80fa\u9176(transglutaminases)\u7684\u4e00\u7a2e\u9ad8\u89aa\u548c\u529b\u5e95\u7269\uff0c\u53ef\u4ee5\u963b\u65b7\u8a31\u591a\u914d\u9ad4\u7684\u53d7\u9ad4\u4ecb\u5c0e\u7684\u5167\u541e\u4f5c\u7528(endocytosis)\u3002Dansylcadaverine (Monodansyl cadaverine, MDC)\u00a0 \u7684\u6291\u5236\u6d3b\u6027\u53cd\u6620\u4e86\u5176\u4f5c\u70ba\u8f49\u8c37\u6c28\u9170\u80fa\u9176(\u00a0transglutaminases)\u5e95\u7269\u7684\u80fd\u529b\u4ee5\u53ca\u7af6\u722d\u6027\u963b\u65b7\u7e96\u7dad\u86cb\u767d\u5206\u5b50(fibrin molecules)\u4ea4\u806f\u7684\u80fd\u529b[2]\u3002dansylcadaverine\u00a0\u662f\u4e00\u7a2e\u967d\u96e2\u5b50\u87a2\u5149\u63a2\u91dd\uff0c\u53ef\u8207\u7d30\u83cc\u8102\u591a\u91a3(bacterial lipopolysaccharide)\u548c\u8102\u8cea A (lipid A)\u7d50\u5408\uff0c\u4e26\u88ab\u5176\u4ed6\u5c0d\u5167\u6bd2\u7d20(endotoxins)\u5177\u6709\u89aa\u548c\u529b\u7684\u5316\u5408\u7269\u7af6\u722d\u6027\u53d6\u4ee3[3]\u3002<\/p>\n

DMSO : 62.5 mg\/mL\u00a0(186.31 mM;\u00a0Need ultrasonic)<\/p>\n

H2<\/sub>O : 1 mg\/mL\u00a0(2.98 mM;\u00a0ultrasonic and warming and heat to 80\u00b0C)<\/p>\n

\"\"<\/p>\n

Description: Dansylcadaverine is an acid dye with green fluorescence, it is a specific marker for autophagic vacuoles, and it can be used for the detection and quantification of autophagy.Method: For Dansylcadaverine staining. \u00a0(\u5f15\u7528\u6587\u737b)\u00a0<\/a><\/span><\/p>\n

1. Cells are seeded in 6-well plates at a concentration of 3\u00d7104 cells\/well in a 5% CO2 incubator at 37\u00b0C for 24 h.
\n2. Following transfection, 50 mM Dansylcadaverine is added to each well for 15 min incubation, and then rinse cells for 3 times with PBS.
\n3. The fluorescence is visualized under a confocal laser-scanning microscope (TCS 4D; Leica, Heidelberg, Germany).<\/p>\n

\"\"<\/p>\n

Description: Dansylcadaverine is an acid dye with green fluorescence, it can be used as a specific autophagolysosome marker to analyze the autophagic process.<\/span>
\n Method: For Dansylcadaverine staining.\u00a0(\u5f15\u7528\u6587\u737b)\u00a0<\/a>\u00a0<\/span><\/p>\n

1. Cells are cultured on confocal dishes at a density of 1\u00d7104 cells\/mL overnight and then added with testing compounds for one hour.<\/span>
\n2. After stimulation with RANKL (50 ng\/mL) for five days, cells are incubated with Dansylcadaverine (50 mM; 15 min; 37\u00b0C).<\/span>
\n3. Wash cells with 1\u00d7PBS for three times with 5 min interval.<\/span>
\n4. Finally, the cells are observed under a confocal laser scanning microscopy.<\/span><\/p>\n

 <\/p>\n\n\n\n\n\n\n\n
Molecular Weight<\/th>\n\n
\n

335.46<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Appearance<\/th>\n\n
\n

Solid<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

Formula<\/th>\n\n
\n

C17<\/sub>H25<\/sub>N3<\/sub>O2<\/sub>S<\/p>\n<\/div>\n<\/td>\n<\/tr>\n

CAS No.<\/th>\n\n
SMILES<\/th>\n\n
\n

NCCCCCNS(=O)(C1=CC=CC2=C1C=CC=C2N(C)C)=O<\/p>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Dansylcadaverine (Monodansyl cadaverine, MDC) \u7d30\u80de\u87a2\u5149\u986f\u5fae\u93e1\u5206\u6790(50 mM)<\/span><\/p>\n

MCF7 cells (2.4\u00d7104 ) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37\u00b0C for 15 min and washed with 1\u00d7PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2]<\/p>\n

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5 Publications Citing Use of MCE Dansylcadaverine<\/span><\/h4>\n<\/div>\n
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