{"id":40316,"date":"2023-03-20T16:38:26","date_gmt":"2023-03-20T08:38:26","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=40316"},"modified":"2025-06-30T15:41:45","modified_gmt":"2025-06-30T07:41:45","slug":"%e5%b8%b8%e8%a6%8b%e7%9a%84%e8%9b%8b%e7%99%bd%e9%85%b6%e5%8f%8a%e7%a3%b7%e9%85%b8%e9%85%b6%e6%8a%91%e5%88%b6%e9%85%b5%e7%b4%a0-protease-and-phosphatase-inhibitor-cocktails","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=40316","title":{"rendered":"\u78f7\u9178\u9176\u6291\u5236\u9175\u7d20Cocktails | Phosphatase Inhibitor Cocktail 2 (100X in ddH2O) \u8ca8\u865fK1013"},"content":{"rendered":"

\u86cb\u767d\u9176\u53ca\u78f7\u9178\u9176\u6291\u5236\u9175\u7d20\u7cfb\u5217\u5546\u54c1->><\/span>Protease and Phosphatase Inhibitor Cocktails<\/span><\/span><\/strong><\/p>\n

APExBIO Technology LLC \u662f\u4e00\u5bb6\u9818\u5148\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5291(Small Molecule Inhibitors)\/\u6d3b\u5316\u5291(Activators)\u3001\u5316\u5408\u7269\u5eab( Compound Libraries)\u3001\u80dc\u80bd(Peptides)\u3001\u6aa2\u6e2c\u8a66\u5291\u76d2(Assay Kits)\u3001\u87a2\u5149\u6a19\u8a18(Fluorescent Label)\u3001\u9176(Enzymes)\u3001\u4fee\u98fe\u6838\u82f7\u9178(Modified Nucleotides)\u3001mRNA \u5408\u6210\u4ee5\u53ca\u5404\u7a2e\u5206\u5b50\u751f\u7269\u5b78\u5de5\u5177\u7684\u4f9b\u61c9\u5546\u3002APExBIO\u63d0\u4f9b\u5ee3\u6cdb\u7684\u7522\u54c1\u7dda\uff0c\u6db5\u84cb 20 \u591a\u500b\u4e0d\u540c\u7684\u7814\u7a76\u9818\u57df\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neurosciences)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u8868\u89c0\u907a\u50b3\u5b78(epigenetics)\u7b49\u3002\u516c\u53f8\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u5fb7\u5dde\u4f11\u58eb\u9813USA (Houston, Texas)\uff0c\u81f4\u529b\u65bc\u670d\u52d9\u5168\u7403\u5ba2\u6236\u3002\u9ad8\u5ea6\u91cd\u8996\u7522\u54c1\u54c1\u8cea\u3002\u6240\u6709\u7522\u54c1\u5747\u9075\u5faa\u56b4\u683c\u7684\u751f\u7522\u6307\u5357\uff0c\u4e26\u9644\u6709\u5206\u6790\u8b49\u66f8\u3001HPLC\u3001\u8cea\u8b5c(Mass Spectrum)\u548cHMNR\u4ee5\u53ca\u9ad4\u5916\u9a57\u8b49(in vitro validation)\u3002 APExBIO\u7522\u54c1\u5df2\u88ab\u300aNature\u300b\u3001\u300aCell\u300b\u548c\u300aScience\u300b\u7b49\u773e\u591a\u9802\u7d1a\u540c\u884c\u8a55\u5be9\u671f\u520a\u5f15\u7528\u3002<\/p>\n

<\/p>\n

\u78f7\u9178\u9176\u6291\u5236\u9175\u7d20Cocktails\uff0cPhosphatase Inhibitor Cocktail 2 (100X in ddH2O)\u00a0|\u00a0<\/strong>\u8ca8\u865fK1013<\/strong><\/a>\u00a0 <\u6291\u5236\u916a\u6c28\u9178\u86cb\u767d\u78f7\u9178\u9176\u3001\u9178\u6027\u548c\u9e7c\u6027\u78f7\u9178\u9176><\/span><\/p>\n

Phosphatase Inhibitor Cocktail 2 (100X in ddH2O)<\/p>\n

\u5728\u5ba4\u6eab\u4e0b\u89e3\u51cd\uff0c\u5728\u6e2c\u5b9a\u524d\u4ee5 1:100 (v\/v) \u7684\u7a00\u91cb\u5ea6\u6dfb\u52a0\u5230\u6eb6\u6db2\u6a23\u54c1\uff08\u4f8b\u5982\u7d30\u80de\u88c2\u89e3\u7269\u6216\u7d44\u7e54\u63d0\u53d6\u7269\uff09\u4e2d\u3002\u61c9\u7528\uff1aWB\u3001Co-IP\u3001pull-down\u3001IF\u3001IHC\u3001kinase assay\u7b49\u3002\u78f7\u9178\u9176\u6291\u5236\u9175\u7d20Cocktails\uff0cPhosphatase Inhibitor Cocktail 2 (100X in ddH2O)\u00a0|\u00a0<\/strong>\u8ca8\u865fK1013<\/strong><\/a>\u00a0<\/span>\u6291\u5236\u916a\u6c28\u9178\u86cb\u767d\u78f7\u9178\u9176\u3001\u9178\u6027\u548c\u9e7c\u6027\u78f7\u9178\u9176\u3002\u9019\u7a2e\u78f7\u9178\u9176\u6291\u5236\u5291\u6df7\u5408\u7269\u5177\u6709\u5c0d\u4f86\u81ea\u5404\u7a2e\u52d5\u7269\u7d44\u7e54\u7684\u7d30\u80de\u63d0\u53d6\u7269\u9032\u884c\u4e86\u512a\u5316\u548c\u6e2c\u8a66\u3002\u9019\u7a2e\u78f7\u9178\u9176\u6291\u5236\u5291\u6df7\u5408\u7269\u5305\u542b\u55ae\u7368\u7684\u6210\u5206\uff0c\u5305\u62ec\u539f\u91e9\u9178\u9209(Sodium orthovanadate)\u3001\u926c\u9178\u9209(Sodium molybdate)\u3001\u9152\u77f3\u9178\u9209(\u00a0Sodium tartrate)\u3001\u54aa\u5511(Imidazole )\u548c\u6c1f\u5316\u9209(Sodium Fluoride)\u3002\u9019\u7a2e\u78f7\u9178\u9176\u6291\u5236\u5291\u6df7\u5408\u7269(phosphatase inhibitor cocktail )\u4ee5 ddH2O \u4e2d\u7684\u5373\u7528\u578b\u6eb6\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

\"\"<\/p>\n

\u86cb\u767d\u9176<\/strong>\u8207\u78f7\u9178\u9176\u4ecb\u7d39<\/strong>:<\/strong>\u00a0<\/span><\/p>\n

Apexbio\u63a8\u51fa\u4e00\u7cfb\u5217\u86cb\u767d\u9176\u53ca\u78f7\u9178\u9176\u96de\u5c3e\u9152\u6291\u5236\u9175\u7d20(Cocktails)\uff0c\u5728\u86cb\u767d\u8cea\u63d0\u53d6\u548c\u7d14\u5316\u671f\u9593\u63d0\u4f9b\u5c0d\u86cb\u767d\u9176\u548c\u78f7\u9178\u9176\u7684\u4e00\u5c64\u4fdd\u8b77\u3002\u86cb\u767d\u8cea\u78f7\u9178\u5316(Protein phosphorylation )\u662f\u4e00\u7a2e\u91cd\u8981\u7684\u5171\u50f9\u7ffb\u8b6f\u5f8c\u4fee\u98fe(covalent post-translational modification)\uff0c\u53ef\u4ee5\u6539\u8b8a\u86cb\u767d\u8cea(protein)\u7684\u7d50\u69cb\u69cb\u8c61\uff0c\u7136\u5f8c\u8abf\u7bc0\u9776\u86cb\u767d\u7684\u529f\u80fd(function)\u3001\u4f4d\u7f6e(location )\u548c\u7279\u7570\u6027\u7d50\u5408(\u00a0specific binding )\u3002\u8a31\u591a\u7d30\u80de\u904e\u7a0b\u53d7\u53ef\u9006\u78f7\u9178\u5316\u7684\u8abf\u7bc0\u86cb\u767d\u8cea\u548c 30% \u7684\u86cb\u767d\u8cea\u5728\u5176\u5b58\u5728\u671f\u9593\u7684\u67d0\u500b\u6642\u523b\u53ef\u80fd\u6703\u88ab\u78f7\u9178\u5316(phosphorylated)\u3002\u5167\u6e90\u6027\u86cb\u767d\u8cea(Endogenous proteins )\u5728\u5e73\u8861\u72c0\u614b\u4e0b\u7522\u751f\u548c\u964d\u89e3\uff0c\u56e0\u6b64\u5b83\u5011\u7684\u7d30\u80de\u6c34\u5e73\u5728\u7a69\u5b9a\u7684\u74b0\u5883\u689d\u4ef6\u4e0b\u662f\u7a69\u5b9a\u7684\u3002\u7c97\u7d30\u80de\u63d0\u53d6\u7269(Crude cell extracts )\u542b\u6709\u8a31\u591a\u5167\u6e90\u6027\u9176\uff0c\u4f8b\u5982\u78f7\u9178\u9176(phosphatases)\u548c\u86cb\u767d\u9176(proteases)\uff0c\u5b83\u5011\u80fd\u5920\u5728\u8403\u53d6\u904e\u7a0b\u4e2d\u964d\u89e3\u548c\u4fee\u98fe\u86cb\u767d\u8cea\u3002\u589e\u52a0\u5b8c\u6574\u86cb\u767d\u8cea\u7522\u91cf\u7684\u6700\u4f73\u65b9\u6cd5\u662f\u6dfb\u52a0\u5df2\u77e5\u5b58\u5728\u9176\u6291\u5236\u5291\u3002<\/p>\n

\"\"<\/p>\n

 <\/p>\n

\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1<\/span>\u00a0Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc\u00a0<\/a>|\u00a0BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n
Catalog No.<\/b><\/td>\nProduct Name<\/b><\/td>\nSummary<\/b><\/td>\nTargets<\/b><\/td>\nCAS Number<\/b><\/td>\n<\/tr>\n
A8524<\/td>\nSodium Orthovanadate<\/a><\/td>\nPTP inhibitor<\/td>\nMembrane Transporter\/Ion Channel|ATPase<\/td>\n13721-39-6<\/td>\n<\/tr>\n
B7843<\/td>\nSodium molybdate<\/a><\/td>\nAcid and phosphoprotein phosphatases inhibitor<\/td>\n<\/td>\n7631-95-0<\/td>\n<\/tr>\n
B7844<\/td>\nSodium tartrate<\/a><\/td>\nAcid phosphatases inhibitor<\/td>\n<\/td>\n868-18-8<\/td>\n<\/tr>\n
B7845<\/td>\nImidazole<\/a><\/td>\nAlkaline phosphatases inhibitor<\/td>\n<\/td>\n288-32-4<\/td>\n<\/tr>\n
B7846<\/td>\nsodium fluoride<\/a><\/td>\nAcid phosphatases inhibitor<\/td>\n<\/td>\n7681-49-4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n

APExBIO\u53f0\u7063\u4ee3\u7406\u5546<\/span><\/strong><\/span><\/a><\/p>\n

APExBIO\u63d0\u4f9b\u512a\u8cea\u7684\u80dc\u80bd\u548c\u751f\u7269\u5206\u6790\u8a66\u5291\uff0c\u5ee3\u6cdb\u7522\u54c1\u7dda\u5982Protease\u3001Chromatin\/Epigenetics\u8868\u89c0\u907a\u50b3\u5b78\u3001MAPK Signal\u3001DNA Damege\/DNA repair\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u816b\u7624\u751f\u7269\u5b78\u3001Stem cell\u7b49\u7814\u7a76\u9818\u57df\uff0c \u9084\u63d0\u4f9b\u5b9a\u5236\u7684\u670d\u52d9\uff0c\u5305\u62ec\u591a\u80bd\u5408\u6210\uff0c\u6297\u9ad4\u7684\u751f\u7522\u548c\u6aa2\u6e2c\u7684\u767c\u5c55\u3002\u7522\u54c1\u7ddaDNA\/RNA prep kit\u3001Drug Screening panel\u3001Bioactive peptides\u3001Growth factors\u3001Biotinylation Reagents\u3001tag peptides\u3001amino Acids\u3001Growth factors\u7b49\u3002APExBIO\u7522\u54c1\u8cea\u91cf\u90fd\u4f34\u96a8\u8457\u8b49\u66f8\u7684\u5206\u6790\uff0c\u9ad8\u6548\u6db2\u76f8\u8272\u8b5c\uff0c\u8cea\u8b5c\uff0c\u548cHMNR\u6578\u64da\u3002<\/p>\n

\"\u578b\u9304\u4e0b\u8f09\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
APExBIO\u7522\u54c1\u7dda<\/span><\/td>\n\u54c1\u9805<\/span><\/td>\nCAS#<\/span><\/td>\n#\u8ca8\u865f<\/span><\/td>\n<\/tr>\n
Proteases<\/span><\/td>\nZ-VAD-FMK | CAS# 187389-52-2 APExBIO\u00a0<\/a><\/span><\/td>\nCAS# 187389-52-2<\/span><\/td>\n\u8ca8\u865f\u00a0A1902<\/span><\/td>\n<\/tr>\n
Proteinase K<\/span><\/td>\n\u86cb\u767d\u9176 K (Proteinase K) | CAS#39450-01-6 APExBIO\u00a0 \u8ca8\u865fK1037<\/a><\/span><\/td>\nCAS#39450-01-6<\/span><\/td>\n\u8ca8\u865fK1037<\/span><\/td>\n<\/tr>\n
\u54c1\u9805<\/span><\/td>\nCAS#<\/span><\/td>\n#\u8ca8\u865f<\/span><\/td>\n\u61c9\u7528<\/span><\/td>\n<\/tr>\n
Romidepsin (FK228, depsipeptide)<\/a><\/span><\/td>\n\n

CAS#128517-07-7<\/span><\/p>\n

 <\/td>\n

\u8ca8\u865fA8173<\/span><\/td>\nHDAC1\/HDAC2\uff0c\u5f37\u6548\u548c\u9078\u64c7\u6027\u6291\u5236\u5291<\/span><\/td>\n<\/tr>\n
Pirarubicin<\/a><\/span><\/td>\nCAS#72496-41-4<\/span><\/td>\n\u8ca8\u865fB2295<\/span><\/td>\n\u00a0anthracycline anti-neoplastic doxorubicin<\/span><\/td>\n<\/tr>\n
Tunicamycin<\/a><\/span><\/td>\nCAS# 15663-27-1<\/span><\/td>\n\n

\u8ca8\u865fA8321<\/span><\/p>\n

 <\/td>\n

\u9ad8\u6548\u3001\u5ee3\u8b5c\u7684\u5316\u7642\u85e5\u7269<\/span><\/td>\n<\/tr>\n
Azithromycin<\/a><\/span><\/td>\nCAS#83905-01-5<\/span><\/td>\n\u00a0\u8ca8\u865fA2213<\/span><\/td>\nHsp90 inhibitor<\/span><\/td>\n<\/tr>\n
Bleomycin Sulfate<\/a><\/span><\/td>\n\n

CAS# 9041-93-4<\/span><\/p>\n

 <\/td>\n

\u8ca8\u865fB1398<\/span><\/td>\nAntibiotic by inhibiting protein synthesis<\/span><\/td>\n<\/tr>\n
Nystatin (Fungicidin)<\/a><\/span><\/td>\nCAS#1400-61-9<\/span><\/td>\n\u8ca8\u865fB1993<\/span><\/td>\nantifungal antibiotic<\/span><\/td>\n<\/tr>\n
Rapamycin<\/a><\/span><\/td>\nCAS#53123-88-9<\/span><\/td>\n\u8ca8\u865fA8167<\/span><\/td>\nOriginal antifungal antibiotic<\/span><\/td>\n<\/tr>\n
Daptomycin<\/a><\/span><\/td>\nCAS#103060-53-3<\/span><\/td>\n\u8ca8\u865fA1206<\/span><\/td>\nCalcium-dependent antibiotic<\/span><\/td>\n<\/tr>\n
\u54c1\u9805<\/span><\/td>\nCAS#<\/span><\/td>\n#\u8ca8\u865f<\/span><\/td>\n\u61c9\u7528<\/span><\/td>\n<\/tr>\n
Z-VAD-FMK \u00a0<\/a><\/span><\/td>\nCAS# 187389-52-2<\/span><\/td>\n\u8ca8\u865f\u00a0A1902<\/span><\/td>\n\n

Proteases<\/span><\/p>\n

ICE-like proteases<\/span><\/td>\n<\/tr>\n

TAPI-1 \u00a0<\/a><\/span><\/td>\nCAS#171235-71-5<\/span><\/td>\n\u8ca8\u865f\u00a0B4686<\/span><\/td>\n\n

Proteases<\/span><\/p>\n

TACE\/ADAM17 inhibitor Product Citation+3<\/span><\/td>\n<\/tr>\n

Talabostat mesylate<\/a><\/span><\/td>\nCAS# 150080-09-4<\/span><\/td>\n\u8ca8\u865fB3941<\/span><\/td>\n\n

Proteases<\/span><\/p>\n

DPP4 \u4e8c\u80bd\u57fa\u80bd\u9176\u7279\u7570\u6027\u6291\u5236\u5291<\/span><\/td>\n<\/tr>\n

Z-IETD-FMK<\/a><\/span><\/td>\nCAS# 210344-98-2<\/span><\/td>\n\u8ca8\u865fB3232<\/span><\/td>\ninhibitor of caspase 8<\/span><\/td>\n<\/tr>\n
Cisplatin<\/span><\/td>\nCAS# 15663-27-1<\/span><\/td>\n\u8ca8\u865fA8321<\/span><\/td>\n\u9ad8\u6548\u3001\u5ee3\u8b5c\u7684\u5316\u7642\u85e5\u7269<\/span><\/td>\n<\/tr>\n
17-DMAG (Alvespimycin) HCl<\/a><\/span><\/td>\nCAS#467214-21-7<\/span><\/td>\n\u00a0\u8ca8\u865fA2213<\/span><\/td>\nHsp90 inhibitor<\/span><\/td>\n<\/tr>\n
CA-074 Me\u00a0<\/a><\/span><\/td>\nCAS# 147859-80-1<\/span><\/td>\n\u8ca8\u865fA8239<\/span><\/td>\nCathepsin B inhibitor<\/span><\/td>\n<\/tr>\n
VX-765<\/a><\/span><\/td>\nCAS# 273404-37-8<\/span><\/td>\n\u8ca8\u865fA8238<\/span><\/td>\nCaspase-1 inhibitor<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u96f7\u5e15\u9ef4\u83cc\uff08Rapamycin\uff09<\/span><\/span><\/span><\/span><\/span><\/a>|\u00a0Romidepsin (FK228, depsipeptide)<\/a>\u00a0|\u00a0Daptomycin\u00a0<\/a>|\u00a0G-418 (80 S \u6838\u91a3\u9ad4\u5ef6\u4f38\u6291\u5236\u5291)<\/a>\u00a0<\/span><\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u86cb\u767d\u9176\u53ca\u78f7\u9178\u9176\u6291\u5236\u9175\u7d20\u7cfb\u5217\u5546\u54c1->>Protease and Phosphatase Inhibi […]<\/p>\n","protected":false},"author":3,"featured_media":34281,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5368],"tags":[5415],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40316"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=40316"}],"version-history":[{"count":8,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40316\/revisions"}],"predecessor-version":[{"id":51797,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/40316\/revisions\/51797"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34281"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=40316"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=40316"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=40316"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}