{"id":39458,"date":"2023-03-05T16:57:48","date_gmt":"2023-03-05T08:57:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=39458"},"modified":"2023-11-16T13:56:53","modified_gmt":"2023-11-16T05:56:53","slug":"%e6%8a%97%e6%b0%a7%e5%8c%96%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90-%e5%b8%b8%e8%a6%8b%e6%8a%97%e6%b0%a7%e5%8c%96%e6%b4%bb%e6%80%a7%e7%9a%84%e8%a9%95%e4%bc%b0%e6%96%b9%e6%b3%95-%e5%a4%aa%e9%bc%8e","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=39458","title":{"rendered":"<<\u6297\u6c27\u5316\u6d3b\u6027\u5206\u6790>> \u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5"},"content":{"rendered":"

\u3010\u6297\u6c27\u5316\u6d3b\u6027\u5206\u6790 \u3011\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span><\/strong><\/span><\/p>\n

\u4eba\u9ad4\u5728\u6b63\u5e38\u4ee3\u8b1d\u904e\u7a0b\u4e2d\u6703\u7522\u751f\u81ea\u7531\u57fa\uff08free radical\uff09\u8207\u6d3b\u6027\u6c27\uff0c\u6297\u6c27\u5316\u5291(Antioxidants)\u5728\u9632\u6b62\u81ea\u7531\u57fa\uff08free radical\uff09\u548c\u5176\u5b83\u6f5b\u5728\u6bd2\u6027\u6c27\u5316\u7269\u8cea\u7684\u5f62\u6210\u548c\u6e05\u9664\u4e2d\uff0c\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002\u6297\u6c27\u5316\u7269\u8cea\u4e09\u5927\u7a2e\u985e: \u6297\u6c27\u5316\u7dad\u751f\u7d20\u7cfb\u7d71&\u5c0f\u5206\u5b50\u3001\u6297\u6c27\u5316\u9175\u7d20\u7cfb\u7d71\u3001\u548c\u86cb\u767d\u8cea\u3002\u6297\u6c27\u5316\u7dad\u751f\u7d20\u7cfb\u7d71&\u5c0f\u5206\u5b50\u5305\u62ec\u6297\u58de\u8840\u9178\u9e7d(ascorbate)\uff0c\u5c3f\u9178(uric acid)\u3001GSH\u3001\u7dad\u751f\u7d20E(Vitamin E, tocopherol -\u751f\u80b2\u915a\uff09\u3001\u985e\u80e1\u863f\u8514\u7d20\uff08\u03b2-carotene\uff09\u3001\u8f14\u9176Q10\u7b49\u3002\u6297\u6c27\u5316\u9175\u7d20\u7cfb\u7d71\u5305\u62ecGSH\u9084\u539f\u9176(GSH reductase)\u3001\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u3001\u904e\u6c27\u5316\u7269\u9176(peroxidase\uff09\u7b49\u3002\u86cb\u767d\u8cea\u5305\u62ec\uff08\u767d\u86cb\u767dalbumin)\u3001transferrin(\u8f49\u9435\u86cb\u767d\u7b49\uff09\u7b49\u3002\u6297\u6c27\u5316\u7269\uff08antioxidants\uff09\u4e4b\u4f5c\u7528\u662f\u6307\u80fd\u6709\u6548\u6291\u5236\u6216\u6e1b\u7de9\u81ea\u7531\u57fa\u6216\u81ea\u7531\u57fa\u6240\u7522\u751f\u4e4b\u6c27\u5316\u93c8\u9396\u53cd\u61c9\u4e4b\u7269\u8cea\u3002\u5728\u4eba\u9ad4\u5167\u6709\u5169\u5927\u6297\u6c27\u5316\u7cfb\u7d71\uff0c\u4e00\u662f\u6297\u6c27\u5316\u7dad\u751f\u7d20\u7cfb\u7d71\uff0c\u53e6\u4e00\u5247\u662f\u6297\u6c27\u5316\u9175\u7d20\u7cfb\u7d71\u3002\u76ee\u524d\u5e38\u7528\u65bc\u5206\u6790\u6297\u6c27\u5316\u6d3b\u6027\u7684\u65b9\u6cd5\u5f88\u591a\uff0c\u5305\u62ec\u6e05\u9664xanthine \/ xanthine oxidase\u6240\u7522\u751f\u4e4b\u8d85\u6c27\u9670\u96e2\u5b50\uff08superoxide anion radical\uff0cO2- \uff09\u7684\u4f5c\u7528\u3001\u6355\u6349\u904e\u6c27\u5316\u6c2b\uff08H2O2\uff09\u7684\u80fd\u529b\u6e2c\u5b9a\u3001\u9084\u539f\u529b\u6e2c\u5b9a\u3001\u6e05\u9664DPPH\uff08\u03b1,\u03b1-diphenyl\u03b2-pricrylhydrazyl\uff09\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u3001\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178\uff08TBA\uff09\u6cd5\u548c\u751f\u7269\u5316\u5b78\u767c\u5149\u6cd5\u6e05\u9664\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u8a55\u4f30\u7b49\u3002<\/p>\n

>>\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5>><\/span><\/strong><\/a><\/p>\n

\"HOT\"<\/a>CheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c | CheKine\u2122 Xanthine Oxidase Assay Kit\u8ca8\u865fKTB1070<\/a><\/span><\/strong><\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/span><\/p>\n

\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08Xanthine oxidase , EC 1.17.3.2\uff09\u6216\u9ec3\u560c\u5464\u6c27\u5316\u9084\u539f\u9176(xanthine oxidoreductase) \u88ab\u8a8d\u70ba\u662f\u560c\u5464(purine)\u5728\u4eba\u9ad4\u5167\u5206\u89e3\u4ee3\u8b1d\u7684\u672b\u7aef\u9176\uff0c\u53ef\u50ac\u5316\u6b21\u9ec3\u560c\u5464(hypoxanthine)\u6c27\u5316\u70ba\u9ec3\u560c\u5464(xanthine)\u7136\u5f8c\u8b8a\u70ba\u5c3f\u9178(uric acid)\u3002 \u7576\u5145\u7576\u70baNADH\u6c27\u5316\u9176 (NADH oxidase) \u6642\uff0cXO\u662f\u8d85\u6c27\u5316\u7269\uff08superoxide , O2-\uff09\u7684\u751f\u6210\u5668(generator)\uff0c\u8d85\u6c27\u5316\u7269\uff08superoxide , O2-\uff09\u662f\u4e00\u7a2e\u5f37\u5927\u7684\u6d3b\u6027\u6c27\uff08reactive oxygen species , ROS\uff09\u3002 \u9084\u5df2\u7d93\u767c\u73feXO\u5728\u7f3a\u8840\u518d\u704c\u6ce8\u640d\u50b7(ischemia-reperfusion injury)\u671f\u9593\u6703\u7522\u751f\u904e\u6c27\u5316\u6c2b\uff08hydrogen peroxide , H2O2\uff09\u548c\u8d85\u6c27\u5316\u7269\uff08superoxide , O2-\uff09\u3002 \u7531\u65bc\u5b83\u5011\u7684\u9ad8\u53cd\u61c9\u6027\uff0c\u9019\u4e9bROS\u5f71\u97ff\u7d30\u80de\u7684\u5404\u7a2e\u5206\u5b50\u6210\u5206\uff0c\u904e\u91cf\u6703\u5c0e\u81f4\u7d30\u80de\u8b8a\u6027(cell degeneration)\u548c\u6b7b\u4ea1(death)\u3002 XO\uff08Xanthine oxidase , EC 1.17.3.2\uff09\u5728\u5065\u5eb7\u500b\u9ad4\u7684\u809d\u81df(liver)\u548c\u7a7a\u8178(jejunum)\u4e2d\u5b58\u5728\u5927\u91cf\u3002 \u4f46\u662f\uff0c\u5728\u5404\u7a2e\u809d\u81df\u75be\u75c5(liver disorders)\u4e2d\uff0c\u5df2\u767c\u73fe\u5faa\u74b0XO\u7684\u6c34\u5e73\u6025\u5287\u589e\u52a0\u3002 \u56e0\u6b64\uff0c\u8840\u6e05XO(serum XO)\u6c34\u5e73\u7684\u6e2c\u5b9a\u53ef\u4f5c\u70ba\u6025\u6027\u809d\u640d\u50b7(acute liver damage), \u5982\u9ec3\u75b8( jaundice\uff09\u7684\u654f\u611f\u6307\u6a19\u3002CheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Xanthine Oxidase Assay Kit, KTB1070) \u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u6cd5\uff0c\u7528\u65bc\u5b9a\u91cf\u6e2c\u5b9a\u5404\u7a2e\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u9ec3\u560c\u5464\u6c27\u5316\u9176 \uff08Xanthine oxidase, XO\uff09\u3002<\/p>\n

\"\"<\/a><\/p>\n

\"HOT\"<\/a>CheKine\u2122\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09CheKine\u2122 Reactive Oxygen Species (ROS) Detection Assay Kit\u00a0| \u8ca8\u865fKTB1910<\/a><\/span><\/strong><\/span><\/p>\n

CheKine\u2122 Reactive Oxygen Species (ROS) Detection Assay Kit (Fluorometric)\u00a010 mM\u00a0 50\u500b\u53cd\u61c9(\u00a010mM, 50ul)<\/span><\/p>\n

\/100\u500b\u53cd\u61c9 (10mM, 100ul)\u00a0 (\u4ee56well, 1ml\u9ad4\u7a4d\u70ba\u4e00\u500b\u53cd\u61c9)<\/p>\n

\u6d3b\u6027\u6c27\uff08Reactive oxygen species, ROS\uff09\u662f\u751f\u7269\u6b63\u5e38\u6709\u6c27\u4ee3\u8b1d\u904e\u7a0b\u4e2d\u4e4b\u526f\u7522\u54c1\uff0c\u5728\u7d30\u80de\u4fe1\u865f\u50b3\u5c0e(cell signaling)\u548c\u9ad4\u5167\u5e73\u8861(vivo balance)\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 \u5728\u66b4\u9732\u65bc\u7d2b\u5916\u7dda\u6216\u71b1\u6e90\u4e0b\uff0cROS\u7684\u91cf\u6703\u6025\u5287\u589e\u591a\u3002\u53ef\u80fd\u662f\u9020\u6210\u660e\u986f\u7684\u7d30\u80de\u7d50\u69cb\u4e4b\u640d\u58de\u3002\u9019\u7a2e\u986f\u73fe\uff0c\u88ab\u7a31\u70ba\u6c27\u5316\u61c9\u6fc0\u3002\u5728\u6c27\u5316\u61c9\u6fc0\u76f8\u95dc\u72c0\u614b(oxidative stress correlation state)\u4e0b\uff0cROS\u6c34\u5e73\u53ef\u4ee5\u5927\u5927\u63d0\u9ad8\u3002\u4e0d\u65b7\u7522\u751f\u4f4e\u6c34\u5e73\u7684\u6d3b\u6027\u6c27\uff08ROS\uff09\u548c\u81ea\u7531\u57fa\u662f\u6240\u6709\u6d3b\u7d30\u80de\u7684\u57fa\u672c\u7279\u5fb5\u3002\u4f4e\u6c34\u5e73\u7684ROS\u5728\u4fe1\u865f\u50b3\u5c0e\u9014\u5f91\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u800c\u5728\u6c27\u5316\u61c9\u6fc0\u4e0bROS\u6d3b\u6027\u589e\u52a0\uff0c\u5c0e\u81f4\u5c0d\u6838\u9178\uff0c\u86cb\u767d\u8cea\u548c\u819c\u8102\u7684\u7834\u58de\u3002 ROS\u5728\u6c27\u5316\u61c9\u6fc0\u904e\u7a0b\u4e2d\u7684\u7a4d\u7d2f\u4e5f\u8207\u8870\u8001\uff0c\u7d30\u80de\u51cb\u4ea1\u6216\u58de\u6b7b\u6709\u95dc\uff0c\u4e26\u8207\u75c5\u7406\u72c0\u614b\u6709\u95dc\uff0cROS\u7684\u7a4d\u7d2f\u5c07\u56b4\u91cd\u7834\u58de\u7d30\u80de\u7d50\u69cb(cell structure)\u3002 \u6c27\u5316\u61c9\u6fc0(Oxidative stress)\u5728\u5fc3\u8840\u7ba1\u75be\u75c5(cardiovascular disease)\uff0c\u7cd6\u5c3f\u75c5(diabetes)\uff0c\u9aa8\u8cea\u758f\u9b06(osteoporosis)\uff0c\u4e2d\u98a8(stroke)\uff0c\u708e\u6027\u75be\u75c5(inflammatory disease)\u4ee5\u53ca\u795e\u7d93\u9000\u884c\u6027\u75be\u75c5(neurodegenerative diseases)\u548c\u764c\u75c7(cancer)\u7b49\u7814\u7a76\u4e2d\u8d77\u8457\u91cd\u8981\u4f5c\u7528\u3002 ROS\u6aa2\u6e2c\u5c07\u6709\u52a9\u65bc\u78ba\u5b9a\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u5982\u4f55\u8abf\u7bc0\u5404\u7a2e\u7d30\u80de\u5167\u9014\u5f91(intracellular pathways)\u3002 ROS\u7684\u7d30\u80de\u6d3b\u6027\u88ab\u6297\u6c27\u5316\u5291\uff0c\u591a\u7a2e\u4fee\u5fa9\u7cfb\u7d71\u548c\u53d7\u640dDNA\u7684\u66ff\u63db\u6240\u62b5\u6d88\u3002<\/p>\n

Preparation of ROS probe: DCFH-DA is diluted with serum-free medium and the final concentration is 10 \u03bcM.\u00a0(\u00a010mM, 50ul\/100ul)<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong>\u00a0<\/span><\/span>CheKine\u2122\u6d3b\u6027\u6c27\uff08CheKine\u2122 Reactive Oxygen Species , ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u5feb\u901f\u7684ROS\u6aa2\u6e2c\u65b9\u6cd5\u3002 ROS\u6aa2\u6e2c\u57fa\u65bc\u87a2\u5149\u63a2\u91ddDCFH-DA\u3002 DCFH-DA\u662f\u4e00\u7a2e\u53ef\u6ef2\u900f\u7d30\u80de\u7684\u9748\u654f\u63a2\u91dd\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u80de\u5167\u6d3b\u6027\u6c27\uff08reactive oxygen species , ROS\uff09\u3002 \u8a72DCFH-DA\u63a2\u91dd\u53ef\u5728\u7a7f\u904e\u6d3b\u7d30\u80de\u819c\u6642\u88ab\u7d30\u80de\u4e2d\u7684\u916f\u9176\u6c34\u89e3\u70baDCFH\u3002 DCFH\u6c92\u6709\u87a2\u5149\u4e26\u4e14\u4e0d\u80fd\u900f\u904e\u7d30\u80de\u819c\uff0c\u7136\u5f8c\u53ef\u4ee5\u88abROS\u6c27\u5316\u4e26\u7522\u751f\u87a2\u5149DCF\u3002 \u7136\u5f8c\u53ef\u4ee5\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u6216\u87a2\u5149\u986f\u5fae\u93e1\u6aa2\u6e2c\u87a2\u5149\uff0c\u4e26\u64da\u6b64\u5206\u6790\u7d30\u80de\u4e2d\u6d3b\u6027\u6c27\uff08CheKine\u2122 Reactive Oxygen Species , ROS\uff09\u7684\u542b\u91cf\u3002<\/p>\n

\"HOT\"<\/a>Abbkine\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fKTB1400<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/p>\n

\u4e00\u6c27\u5316\u6c2e\u5408\u9176\uff08EC 1.14.13.39\uff09\uff08NOS\uff09\u662f\u50ac\u5316L-\u7cbe\u6c28\u9178\u751f\u7522\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u7684\u4e00\u985e\u9176\u3002\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u5728\u795e\u7d93\u50b3\u905e\uff0c\u8840\u7ba1\u8abf\u7bc0\uff0c\u514d\u75ab\u61c9\u7b54\u548c\u51cb\u4ea1\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u5728NADPH\uff0cFAD\uff0cFMN\uff0c\uff086R\uff09-5,6,7,8-\u56db\u6c2b\u751f\u7269\u558b\u5464\uff0c\u9223\u8abf\u86cb\u767d\u548c\u8840\u7d05\u7d20\u7684\u5b58\u5728\u4e0b\uff0cNOS\u7528\u5206\u5b50\u6c27\u50ac\u5316L-\u7cbe\u6c28\u9178\u7684\u80cd\u57fa\u6c2e\u7684\u4e94\u96fb\u5b50\u6c27\u5316\uff0c\u7522\u751fNO\u548cL\u74dc\u6c28\u9178\u3002 NOS\u6709\u4e09\u7a2e\u540c\u7a2e\u578b\uff1a\u5167\u76ae\uff08eNOS\uff09\uff0c\u795e\u7d93\u5143\uff08nNOS\uff09\u548c\u8a98\u5c0e\u578b\uff08iNOS\uff09\u3002 nNOS\u4f54\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71NO\u7684\u7522\u751f\uff0c\u5176\u4e2dNO\u53c3\u8207\u7d30\u80de\u901a\u4fe1\u548c\u4fe1\u606f\u5b58\u5132\u3002 eNOS\u5728\u8840\u7ba1\u4e2d\u7522\u751fNO\uff0c\u4e26\u53c3\u8207\u8840\u7ba1\u529f\u80fd\u7684\u8abf\u7bc0\u3002\u8207\u5176\u4ed6\u7570\u69cb\u9ad4\u76f8\u53cd\uff0ciNOS\u5728\u6c27\u5316\u61c9\u6fc0\u689d\u4ef6\u4e0b\u5f9e\u982d\u986f\u793a\uff0c\u4e26\u7522\u751f\u5927\u91cf\u7684NO\u4f5c\u70ba\u8eab\u9ad4\u9632\u79a6\u6a5f\u5236\u7684\u4e00\u90e8\u5206\u3002<\/p>\n

\u4e00\u6c27\u5316\u6c2e(NO)\u662f\u4e00\u7a2e\u53cd\u61c9\u6027\u81ea\u7531\u57fa\uff0c\u5728\u795e\u7d93\u50b3\u905e\u3001\u514d\u75ab\u53cd\u61c9\u3001\u7d30\u80de\u51cb\u4ea1\u7b49\u91cd\u8981\u751f\u7406\u529f\u80fd\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002\u4e00\u6c27\u5316\u6c2e(NO)\u662f\u7531L-\u7cbe\u6c28\u9178\u7d93NO\u5408\u9176(NOS)\u5408\u6210\u7684\uff0c\u5df2\u88ab\u9452\u5b9a\u70ba\u5167\u76ae\u6e90\u6027\u8212\u5f35\u56e0\u6578(EDRF)\u548c\u6297\u8840\u5c0f\u677f\u7269\u8cea\u3002\u5b83\u662f\u7531\u4e2d\u6027\u7c92\u7d30\u80de\u548c\u6d3b\u5316\u5de8\u566c\u7d30\u80de\u7522\u751f\u7684\u795e\u7d93\u905e\u8cea\u548c\u7d30\u80de\u6bd2\u6027\u7269\u8cea\u3002\u96d6\u7136\u4e00\u6c27\u5316\u6c2e(NO)\u5728\u751f\u7269\u7cfb\u7d71\u4e2d\u7684\u5206\u5b50\u4f5c\u7528\u662f\u591a\u7a2e\u591a\u6a23\u7684\uff0c\u4f46\u4e00\u6c27\u5316\u6c2e(NO)\u6700\u91cd\u8981\u7684\u4f5c\u7528\u662f\u555f\u52d5\u9ce5\u82f7\u9178\u74b0\u5316\u9176\u3002
\nAbbkine \u4e00\u6c27\u5316\u6c2e(NO)\uff08Nitric Oxide Assay Kit , NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u61c9\u7528\u539f\u7406\u70ba\u4e00\u6c27\u5316\u6c2e(NO) \u88ab\u6c27\u5316\u6210\u4e9e\u785d\u9178\u9e7d\u548c\u785d\u9178\u9e7d\uff0c\u6e2c\u5b9a\u4e9e\u785d\u9178\u9e7d\u548c\u785d\u9178\u9e7d\u7684\u7e3d\u91cf\u4f86\u7b97\u4e00\u6c27\u5316\u6c2e(NO)\u7e3d\u91cf\u3002 \u63a1\u7528\u6539\u826fGriess \u65b9\u6cd5\u5c07\u785d\u9178\u9e7d\u9084\u539f\u70ba\u4e9e\u785d\u9178\u9e7d\u5f8c\uff0c\u6e96\u78ba\u6e2c\u5b9a \u4e00\u6c27\u5316\u6c2e(NO)\u7684\u91cf\u3002Griess\u5206\u6790\u6a5f\u7406\u70ba\u91cd\u6c2e\u985e\u4e4b\u9593\u7684\u5076\u6c2e\u8026\u5408\uff0c\u91cd\u6c2e\u985e\u662f\u7531\u78fa\u80fa(sulfanilamide)\u548c NO2-\u548c N-\uff081-\u8418\u57fa\uff09\u4e59\u4e8c\u6708\u5b89\u4e8cyan\u9178\u9e7d\u7522\u751f\u7684\uff0c\u7522\u751f\u8207 \u4e00\u6c27\u5316\u6c2e(NO)\u4ee3\u8b1d\u7269\u6210\u6b63\u6bd4\u7684\u6bd4\u8272\uff08540 nm\uff09\u7522\u7269\u3002\u8a72\u53cd\u61c9\u539f\u7406\u7684\u512a\u52e2\uff1aAbbkine\u7684\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u9996\u5148\u5c07\u785d\u9178\u9e7d\u9084\u539f\u70ba\u4e9e\u785d\u9178\u9e7d\uff0c\u7d50\u679c\u66f4\u70ba\u6e96\u78ba\u3002\u4e00\u4e00\u6c27\u5316\u6c2e(NO)\u6eb6\u65bc\u6db2\u9ad4\u5f8c\u6703\u751f\u6210\u4e9e\u785d\u9178\u9e7d\u548c\u785d\u9178\u9e7d\uff0c\u4e9e\u785d\u9178\u9e7d\u548c\u785d\u9178\u9e7d\u7684\u76f8\u5c0d\u542b\u91cf\u8b8a\u5316\u5f88\u5927\uff0c\u6700\u6e96\u78ba\u78ba\u5b9a\u7e3d\u4e00\u6c27\u5316\u6c2e(NO)\u91cf\uff0c\u9700\u540c\u6642\u6e2c\u91cf\u785d\u9178\u9e7d\u548c\u4e9e\u785d\u9178\u9e7d\u3002\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/p>\n

\"\"<\/a><\/p>\n

Fig. Standard Curve of Nitric Oxide Assay. The y-axis is OD540 and the x-axis is NO2- concentration (\u03bcM).<\/p>\n

\u9078\u64c7Abbkine\u4e00\u6c27\u5316\u6c2e(NO)\u6aa2\u6e2c\u8a66\u5291\u76d2\u7684\u7406\u7531\uff1a<\/span><\/strong><\/span>
\n1. \u6027\u50f9\u6bd4\u9ad8\uff0c\u7bc0\u7d04\u5be6\u9a57\u6210\u672c\uff1b
\n2. \u90e8\u5206\u6a23\u672c\u4e0d\u9700\u8981\u9810\u8655\u7406\uff0c\u5c3f\u6db2\u548c\u553e\u6db2\u4e0d\u9700\u8981\u812b\u86cb\u767d\uff1b
\n3. \u53cd\u61c9\u689d\u4ef6\u6eab\u548c\uff0c\u9700\u8981\u7684\u5100\u5668\u6210\u672c\u4f4e\uff1b
\n4. \u6709\u591a\u500b\u898f\u683c\uff0c\u5ba2\u6236\u53ef\u4ee5\u6309\u9700\u9078\u64c7\uff1b
\n5. \u5feb\u901f\u53ef\u9760\uff0c\u4f7f\u7528\u512a\u5316\u7684VCl3\u8a66\u5291\uff0c\u5c07NO3 -\u9084\u539f\u70baNO2 -\u6240\u9700\u6642\u9593\u66f4\u77ed\uff1b
\n6. \u8a72\u65b9\u6cd5\u7c21\u55ae\u3001\u9ad8\u901a\u91cf\uff0c\u5c07\u6a23\u54c1\u8207\u8a66\u5291\u6df7\u5408\uff0c\u5b75\u80b2\u4e26\u8b80\u53d6\u5149\u5bc6\u5ea6\uff0c\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u81ea\u52d5\u6e2c\u91cf\u6210\u5343\u4e0a\u842c\u7684\u6a23\u54c1\u3002<\/p>\n

Nitric Oxide (NO)\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/strong><\/span><\/p>\n

\u5546\u54c1\u9700\u914d\u5408\u5100\u5668\u4f7f\u7528\uff0cELISA\u5100\u5668\u8a2d\u5b9a\u6ce2\u9577\u70ba\u00a0540 nm\u3002\u6216\u5206\u5149\u5206\u5ea6\u8a08\u4f7f\u7528(\u5fae\u91cfCuvette)<\/p>\n

\"HOT\"<\/a><\/span>Catalase\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176<\/em>\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Catalase Activity Assay Kit\u8ca8\u865f<\/strong>KTB1040<\/a><\/span><\/p>\n

\u3010Catalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\/\u89f8\u9176\u6027\u6aa2\u6e2c\u3011<\/span><\/strong><\/span>CheKine\u2122 Catalase Activity Assay Kit<\/span><\/span><\/p>\n

\u6e2c\u91cf\u7dda\u6027\u7bc4\u570d\u70ba 2-75 \u00b5M, \u6700\u4f4e\u6e2c\u91cf\u6d3b\u6027\u70ba2 U\/ml\u3002<\/span><\/span><\/p>\n

\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176\uff08Catalase , CAT\uff09\u662f\u5e7e\u4e4e\u5728\u6240\u6709\u6d3b\u751f\u7269\u9ad4\u4e2d\u5e38\u898b\u7684\u6297\u6c27\u5316\u9176(antioxidant enzyme)\u3002\u5b83\u7684\u4e3b\u8981\u529f\u80fd\u662f\u50ac\u5316\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u5206\u89e3\u70ba\u6c34(water)\u548c\u6c27\u6c23(oxygen)\uff0c\u4e26\u53bb\u9664\u9ad4\u5167\u7684\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\uff0c\u5f9e\u800c\u9632\u6b62\u7d30\u80de\u88ab\u904e\u6c27\u5316\u6c2b\u4e2d\u6bd2\u3002\u5b83\u662f\u751f\u7269\u9632\u79a6\u7cfb\u7d71(biological defense system)\u4e2d\u7684\u95dc\u9375\u9176\u4e4b\u4e00\uff0c\u70ba\u4eba\u9ad4\u63d0\u4f9b\u4e86\u6297\u6c27\u5316\u9632\u79a6\u6a5f\u5236(antioxidant defense mechanism)\u3002<\/p>\n

\"\"<\/a><\/p>\n

Fig. Formaldehyde standard curve. The y-axis is absorbance of standards and the x-axis is final formaldehyde concentration (uM).<\/p>\n

CheKine\u2122\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Catalase Activity Assay Kit, KTB1040) \u53ef\u61c9\u7528\u65bc\u5404\u7a2e\u751f\u7269\u6a23\u54c1\uff08\u4f8b\u5982Tissue Homogenate, Cell Lysate, serum, plasma, erythrocyte lysate\uff09\u4e2d\u7684\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027(catalase activity)\u6aa2\u6e2c, \u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u65b9\u6cd5\u3002 \u8a72\u6aa2\u6e2c\u8a66\u5291\u76d2\u57fa\u65bc\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u8207\u7532\u9187(methanol)\u7684\u53cd\u61c9\uff0c\u5728\u6700\u4f73\u6fc3\u5ea6\u7684H2O2\u5b58\u5728\u4e0b\uff0c\u5229\u7528\u904e\u6c27\u5316\u6c2b\u9176(catalase)\u7684\u904e\u6c27\u5316\u529f\u80fd\u4f86\u6e2c\u91cf\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u3002 \u7522\u751f\u7684\u7532\u919b(formaldehyde)\u53ef\u4ee5\u5728OD 540 nm\u8655\u9032\u884c\u6bd4\u8272\u6e2c\u5b9a\u3002 \u56e0\u6b64\uff0c\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027(catalase activity)\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6b63\u6bd4\u3002\u6e2c\u91cf\u7dda\u6027\u7bc4\u570d\u70ba 2-75 \u00b5M, \u6700\u4f4e\u6e2c\u91cf\u6d3b\u6027\u70ba2 U\/ml\u3002<\/span><\/p>\n

\"HOT\"<\/a><\/strong><\/span>\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c\u00a0|\u00a0CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/strong>\u00a0<\/span>\u8ca8\u865f<\/strong>\u00a0KTB1600<\/p>\n

2-200ug\/mL<\/span><\/p>\n

\u7a40\u80f1\u7518\u80bd (Glutathione) \u7814\u7a76\u7684\u610f\u7fa9<\/span><\/strong><\/span><\/p>\n

\u7a40\u80f1\u7518\u80bd\u662f\u542b\u6709\u8c37\u6c28\u9178 (glutamate)\uff0c\u534a\u80f1\u6c28\u9178(cysteine)\u548c\u7518\u6c28\u9178(glycine)\u7684\u4e09\u80bd\uff0c\u5176\u4e2d\u542b\u6709\u5df0\u57fa(sulfhydryl groups)\u3002\u5b83\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u690d\u7269\u4e2d\u3002\u5b83\u5177\u6709\u6297\u6c27\u5316\u5291(antioxidant)\u548c\u7d9c\u5408\u89e3\u6bd2(detoxification)\u4f5c\u7528\u3002\u5176\u76f8\u95dc\u7684\u8f14\u9176(coenzyme)\u662fNADPH\u3002\u4eba\u9ad4\u65b0\u9673\u4ee3\u8b1d\u7522\u751f\u7684\u81ea\u7531\u57fa(free radicals)\u904e\u591a\u6703\u7834\u58de\u751f\u7269\u819c(biofilm)\uff0c\u4fb5\u5165\u751f\u547d\u5927\u5206\u5b50\uff0c\u52a0\u901f\u4eba\u9ad4\u8870\u8001(aging)\uff0c\u4e26\u8a98\u767c\u816b\u7624(tumors)\u6216\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis) \u7684\u7522\u751f\u3002\u7a40\u80f1\u7518\u80bd\u5177\u6709\u589e\u5f37\u751f\u7269\u9ad4\u7684\u6297\u6c27\u5316\u529f\u80fd\uff0c\u4fc3\u9032\u751f\u9577\u4ee5\u53ca\u589e\u5f37\u751f\u7269\u9ad4\u5c0d\u4eba\u9ad4\u4e0d\u5229\u74b0\u5883\u7684\u5f71\u97ff\u7684\u529f\u80fd\u3002\u5b83\u7684\u4e3b\u8981\u751f\u7406\u4f5c\u7528\u662f\u6e05\u9664\u9ad4\u5167\u7684\u81ea\u7531\u57fa\u3002\u5b83\u4e5f\u662f\u4e00\u7a2e\u5f88\u597d\u7684\u89e3\u6bd2\u5291\uff0c\u53ef\u4ee5\u6e1b\u5f31\u6709\u6bd2\u7269\u8cea\u7684\u6bd2\u6027\u4e26\u5f9e\u9ad4\u5167\u6e05\u9664\u6709\u6bd2\u7269\u8cea\u3002\u7a40\u80f1\u7518\u80bd\u9084\u4fc3\u9032\u751f\u9577\u4e26\u63d0\u9ad8\u4eba\u9ad4\u514d\u75ab\u529b\u3002\u7a40\u80f1\u7518\u80bd(Glutathione)\u4e0d\u50c5\u53ef\u4ee5\u7528\u4f5c\u85e5\u7269\uff0c\u9084\u53ef\u4ee5\u7528\u4f5c\u529f\u80fd\u6027\u98df\u54c1\u7684\u57fa\u790e\u6750\u6599\uff0c\u4e26\u4e14\u5ee3\u6cdb\u7528\u65bc\u529f\u80fd\u6027\u98df\u54c1\u4e2d\uff0c\u4f8b\u5982\u6297\u8870\u8001(anti-aging)\uff0c\u589e\u5f37\u514d\u75ab\u529b(enhancing immunity)\u548c\u6297\u816b\u7624\u4f5c\u7528(anti-tumor)\u3002<\/p>\n

\u7a40\u80f1\u7518\u80bd(Glutathione) \u662f\u7518\u6c28\u9178(glycine)\uff0c\u8c37\u6c28\u9178(glutamic)\u548c\u534a\u80f1\u6c28\u9178(cysteine)\u7684\u4e09\u80bd(tripeptide)\u3002\u5728\u7d05\u8840\u7403\u7d30\u80de(red blood cell)\u4e2d\uff0c\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\u662f\u4fdd\u6301\u8840\u7d05\u86cb\u767d\u8655\u65bc\u9084\u539f\u72c0\u614b\u4e26\u4fdd\u8b77\u7d30\u80de\u514d\u53d7\u6c27\u5316\u640d\u50b7(oxidative damage)\u7684\u95dc\u9375\u3002GSH\u662f\u7d30\u80de\u4e2d\u6700\u91cd\u8981\u7684\u6297\u6c27\u5316\u5291\u5df0\u57fa\u5316\u5408\u7269(antioxidant sulfhydryl compound)\uff0c\u5728\u6297\u6c27\u5316(oxidation resistance)\uff0c\u86cb\u767d\u8cea\u5df0\u57fa\u4fdd\u8b77(protein sulfhydryl protection)\u548c\u6c28\u57fa\u9178\u8de8\u819c\u904b\u8f38\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u9084\u539f\u578b(reduced glutathione)\u548c\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd(oxidized glutathione)\u4e4b\u9593\u7684\u6bd4\u4f8b\uff08GSH \/ GSSG\uff09\u662f\u7d30\u80de\u6c27\u5316\u9084\u539f\u72c0\u614b\u7684\u4e3b\u8981\u6307\u6a19\u3002\u56e0\u6b64\uff0c\u6e2c\u91cf\u7d30\u80de\u4e2dGSH\u548cGSSG\u7684\u542b\u91cf\uff0c\u4ee5\u53caGSH \/ GSSG\u7684\u6bd4\u503c\u53ef\u4ee5\u53cd\u6620\u7d30\u80de\u7684\u6c27\u5316\u9084\u539f\u72c0\u614b\uff0c\u4f46\u662f\u5176\u4ed6\u5177\u6709\u904e\u6c27\u5316\u6d3b\u6027\u7684\u9176\u6c92\u6709\u5229\u7528\u9019\u4e9b\u5e95\u7269\u3002DTNB\u8207\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd(reduced glutathione) \u53cd\u61c9\u5f62\u6210\u9ec3\u8272\u7522\u7269\u3002\u5728412 nm\u8655\u6e2c\u5f97\u7684\u5149\u5bc6\u5ea6\u53ef\u4ee5\u76f4\u63a5\u53cd\u6620\u6a23\u54c1\u4e2d\u7684\u7a40\u80f1\u7518\u80bd( glutathione)\u6fc3\u5ea6\u3002<\/p>\n

\"HOT\"<\/a>\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c | \u00a0Glutathione Oxidized (GSSG) Colorimetric Assay Kit \u8ca8\u865f KTB1610<\/a>\u00a0<\/span><\/strong><\/p>\n

1-20 uM<\/span><\/p>\n

\"\"<\/p>\n

GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u6aa2\u6e2c| Glutathione Reductases (GR) Colorimetric Assay Kit\u8ca8\u865fKTB1620<\/u><\/a><\/span><\/p>\n

\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases<\/span>, GR\uff09GR\u662f\u4e00\u7a2e\u9ec3\u7d20\u86cb\u767d\u6c27\u5316\u9084\u539f\u9176\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u771f\u6838\u751f\u7269\u548c\u539f\u6838\u751f\u7269\u4e2d\u3002<\/span>\u00a0\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u50ac\u5316GSSG\u9084\u539f\u70baGSH\u3002<\/span>\u00a0\u5b83\u662f\u7a40\u80f1\u7518\u80bd\u6c27\u5316\u9084\u539f\u5faa\u74b0\u4e2d\u7684\u95dc\u9375\u9176\u4e4b\u4e00\uff08\u901a\u5e38\u5728\u6606\u87f2\u4e2dGR\u88abTrxR\u4ee3\u66ff\uff09\u3002<\/span>\u00a0\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u50ac\u5316NADPH\u5411GSH\u9084\u539fGSSG\uff0c\u6709\u52a9\u65bc\u7dad\u6301\u9ad4\u5167GSH \/ GSSG\u7684\u6bd4\u7387\u3002<\/span>\u00a0\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u5728\u97ff\u61c9\u6c27\u5316\u61c9\u6fc0\u6e05\u9664\u6d3b\u6027\u6c27\u65b9\u9762\u8d77\u8457\u95dc\u9375\u4f5c\u7528\uff0c\u4e26\u4e14\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u4e5f\u53c3\u8207\u6297\u58de\u8840\u9178-\u7a40\u80f1\u7518\u80bd\u5faa\u74b0\u9014\u5f91\u3002\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09<\/span><\/span>\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539f\u6210GSSG\uff0c\u5f9e\u800c\u518d\u751fGSH\uff0cNADPH\u812b\u6c2b\u751f\u6210NADP +\u3002<\/span>\u00a0NADPH\u5728340 nm\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u5728\u8a72\u6ce2\u9577\u6c92\u6709\u5438\u6536\u5cf0\u3002<\/span>\u00a0\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u7387\u4f86\u8a08\u7b97\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08Glutathione Reductases, GR\uff09\u6d3b\u6027\uff0c\u5f9e\u800c\u78ba\u5b9aNADPH\u812b\u6c2b\u7387\u3002<\/span><\/p>\n

\"HOT\"<\/a><\/span>CheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2| CheKine\u2122 Lipid Peroxidation (MDA) Assay Kit, MDA\u8ca8\u865fKTB1050\u00a0<\/strong><\/span><\/p>\n

1-100 \u00b5M\/L<\/p>\n

\u6c27\u5316\u61c9\u6fc0(Oxidative stress)\u8207\u5305\u62ec\u5fc3\u8840\u7ba1\u75be\u75c5(chronic diseases)\u5728\u5167\u7684\u8a31\u591a\u6162\u6027\u75be\u75c5\u7684\u75c5\u56e0\u6709\u95dc\u3002 \u8102\u8cea\u904e\u6c27\u5316(Lipid peroxidation)\u662f\u7531\u65bc\u6c27\u5316\u640d\u50b7(oxidative damage)\u800c\u767c\u751f\u7684\u8102\u8cea(lipids)\u964d\u89e3(degradation)\uff0c\u662f\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u7684\u6709\u7528\u6a19\u8a18(useful marker)\u3002 \u591a\u4e0d\u98fd\u548c\u8102\u8cea(Polyunsaturated lipids)\u901a\u5e38\u6613\u53d7\u6d3b\u6027\u6c27(reactive oxygen)\u7684\u5f71\u97ff\u800c\u53d7\u5230\u6c27\u5316\u653b\u64ca\uff0c\u5f9e\u800c\u5c0e\u81f4\u7522\u751f\u660e\u78ba\u7684\u93c8\u53cd\u61c9\uff0c\u4e26\u751f\u6210\u6700\u7d42\u7522\u7269\uff0c\u5982\u4e19\u4e8c\u919b\uff08malondialdehyde ,MDA\uff09\u8102\u8cea\u904e\u6c27\u5316(Lipid peroxidation)\u53ef\u80fd\u5c0e\u81f4\u8a31\u591a\u75be\u75c5\u7684\u75c5\u7406\uff0c\u5305\u62ec\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\uff0c\u7cd6\u5c3f\u75c5(diabetes)\u548c\u963f\u723e\u8328\u6d77\u9ed8\u6c0f\u75c5(Alzheimer\u2019s)\u3002<\/p>\n

CheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\uff08CheKine\u2122 Lipid Peroxidation (MDA) Assay Kit, MDA\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u70ba\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u4e19\u4e8c\u919b\uff08malondialdehyde, MDA\uff09\u63d0\u4f9b\u4e86\u4fbf\u5229\u7684\u5de5\u5177\u3002 MDA\u82074-\u7fa5\u57fa\u58ec\u70ef\u919b\uff084-hydroxynonenal , 4-HNE\uff09\u4e00\u8d77\u662f\u8102\u8cea\u904e\u6c27\u5316(lipid peroxidation)\u4f5c\u7528\u7684\u5929\u7136\u526f\u7522\u7269\uff0c\u5176\u5b9a\u91cf\u901a\u5e38\u7528\u4f5c\u8102\u8cea\u904e\u6c27\u5316(lipid peroxidation)\u4f5c\u7528\u7684\u6a19\u8a18\u3002 \u6a23\u54c1\u4e2d\u7684MDA\u8207\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178\uff08thiobarbituric acid ,TBA\uff09\u53cd\u61c9\u751f\u6210MDA-TBA\u52a0\u5408\u7269\uff0c\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u5728OD 532 nm\u8655\u9032\u884c\u6bd4\u8272\u5b9a\u91cf\u3002 \u540c\u6642\uff0c\u6e2c\u91cf600nm\u8655\u7684\u5438\u5149\u5ea6\uff0c\u4e26\u901a\u904e532nm\u548c600nm\u8655\u7684\u5438\u5149\u5ea6\u4e4b\u5dee\u8a08\u7b97MDA\uff08\u4ee5\u907f\u514d\u8517\u7cd6(saccharose) \u7684\u5e72\u64fe\uff09\u30021-100 \u00b5M\/L<\/p>\n

CheKine\u2122 Lipid Peroxidation (MDA) Assay Kit provides a convenient tool for detection of the malondialdehyde (MDA) present in a variety of samples. MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as marker for lipid peroxidation. The MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct\uff0cwhich can be easily quantified colorimetrically at OD 532 nm. Meanwhile, the absorbance at 600 nm was measured , and MDA was calculated by the difference between the absorbance at 532 nm and 600 nm (To avoid the interference of saccharose).<\/p>\n

\"\"<\/a><\/p>\n

Fig. Typical MDA standard calibration curve. The y-axis is \u0394OD and the x-axis is MDA concentration (uM).<\/p>\n

\"HOT\"<\/a><\/span><\/strong>GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c |Glutathione Peroxidase (GPx\/GSH-Px) Activity Assay Kit\u8ca8\u865f KTB1640<\/a>\u00a0(340nm)<\/span><\/strong><\/span><\/p>\n

CheKine\u2122 Micro Glutathione Peroxidase (GSH-Px) Activity Assay Kit<\/p>\n

Glutathione peroxidase<\/span>\u00a0(GSH-Px)\uff0c\u7c21\u7a31\u70baGSH-Px\u6216GPx<\/p>\n

Determination of GSH-Px activity in serum, plasma, plant tissue\/cell lysates and other biological fluids.<\/p>\n

\u7a40\u80f1\u7518\u80bd (Glutathione) \u7814\u7a76\u7684\u610f\u7fa9<\/span><\/strong><\/span><\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong><\/span><\/p>\n

GSH-Px \/GPx\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione peroxidase<\/span>\u00a0(GSH-Px)\uff0c\u7c21\u7a31\u70baGSH-Px<\/b>\u6216GPx\u3002<\/b>\u662f\u5728\u7a40\u80f1\u7518\u80bd\u6c27\u5316\u9084\u539f\u5faa\u74b0\u4e2d\u50ac\u5316\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\u6c27\u5316\u7684\u4e3b\u8981\u9176\u4e4b\u4e00\u3002 GSH-Px \/GPx\u3010\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione Peroxidase (GSH-Px)\u3011\u4e0d\u50c5\u80fd\u7279\u7570\u6027\u50ac\u5316\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u8207ROS\u4e4b\u9593\u7684\u53cd\u61c9\uff0c\u5f62\u6210\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bdGSSG\uff0c\u5f9e\u800c\u4fdd\u8b77\u751f\u7269\u819c\u514d\u53d7ROS\u7834\u58de\u4e26\u7dad\u6301\u7d30\u80de\u7684\u6b63\u5e38\u529f\u80fd\u3002\u5b83\u9084\u53ef\u4ee5\u4fdd\u8b77\u809d\u81df\uff0c\u63d0\u9ad8\u4eba\u9ad4\u514d\u75ab\u529b\uff0c\u5c0d\u6297\u6709\u5bb3\u91d1\u5c6c\u96e2\u5b50(metal ions)\u5c0d\u4eba\u9ad4\u7684\u6709\u5bb3\u5f71\u97ff\uff0c\u4e26\u589e\u5f37\u4eba\u9ad4\u62b5\u6297\u8f3b\u5c04(radiation)\u7684\u80fd\u529b\u3002GSH-Px \u3010\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione Peroxidase (GSH-Px \/GPx)\u3011\u50ac\u5316H2O2\u6c27\u5316GSH\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\uff0c\u751f\u6210GSSG (\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bdGSSG)\u3002\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08glutathione reductase , GR\uff09\u50ac\u5316NADPH\u9084\u539fGSSG(\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bdGSSG)\uff0c\u5f9e\u800c\u518d\u751fGSH\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08reduced glutathione , GSH\uff09\uff0c\u800cNADPH\u6c27\u5316\u7522\u751fNADP +\u3002 NADPH\u5728340 nm\u8655\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u6c92\u6709\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4f86\u78ba\u5b9aNADPH\u812b\u6c2b\u901f\u7387\uff0c\u4ee5\u8a08\u7b97GSH-Px \u3010\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176,\u00a0Glutathione Peroxidase (GSH-Px \/GPx)\u3011\u6d3b\u6027\u3002<\/p>\n

GSH-Px catalyzes H2O2 to oxidize GSH to produce GSSG; glutathione reductase (GR) catalyzes NADPH to reduce GSSG to regenerate GSH, while NADPH oxidizes to produce NADP+; NADPH has a characteristic absorption peak at 340 nm, while NADP+ does not; NADPH dehydrogenation rate is determined by measuring the decrease rate of absorbance at 340 nm to calculate GSH-Px activity.<\/p>\n

\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c<\/a>\u00a0|<\/span>\u00a0\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/a>\u00a0|<\/span>\u00a0GR\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u6aa2\u6e2c<\/a>\u00a0|\u00a0<\/span>GST\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\u5206\u6790<\/a>\u00a0|\u00a0<\/span>GSH-Px \u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c<\/a>\u00a0|<\/span>\u00a0\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176 (TrxR) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0<\/span>\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/p>\n

\"HOT\"<\/a><\/span><\/strong>CheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTB120<\/a>0<\/span><\/strong><\/span><\/p>\n

CheKine\u2122 Protein Carbonyl Colorimetric Assay Kit<\/p>\n

\u7fb0\u57fa\u86cb\u767d(Protein carbonyl)\u662f\u86cb\u767d\u8cea\u6c27\u5316\u4fee\u98fe(protein oxidative modification)\u904e\u7a0b\u4e2d\u591a\u7a2e\u6c28\u57fa\u9178(amino acids)\u7684\u65e9\u671f\u4fe1\u865f\uff0c\u5176\u542b\u91cf\u8868\u660e\u4e86\u86cb\u767d\u8cea\u7684\u6c27\u5316\u640d\u50b7\u7a0b\u5ea6(protein oxidative damage degree)\uff0c\u662f\u8861\u91cf\u86cb\u767d\u8cea\u6c27\u5316\u640d\u50b7(protein oxidative damage)\u7684\u4e3b\u8981\u6307\u6a19(indicator)\u3002\u7fb0\u57fa(carbonyl group)\u82072,4-\u4e8c\u785d\u57fa\u82ef(2,4-dinitrophenylhydrazine )\u53cd\u61c9\u751f\u6210\u7d05\u8272\u76842,4-\u4e8c\u785d\u57fa\u82ef(2,4-dinitrophenylhydrazone)\uff0c\u5728370 nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002\u64cd\u4f5c\u624b\u518a\u00a0CheKine\u2122 Protein Carbonyl Colorimetric Assay Kit<\/a><\/p>\n

\"HOT\"<\/a><\/span><\/strong>\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2|\u00a0<\/strong>\u8ca8\u865f<\/strong>KTB1080<\/a><\/span><\/p>\n

CheKine\u2122 Superoxide anion Scavenging Capacity Assay Kit<\/p>\n

\u8d85\u6c27\u9670\u96e2\u5b50\uff08superoxide anion , O2-\uff09\u662f\u901a\u904e\u5c07\u96fb\u5b50\u52a0\u5230\u6c27\u4e2d\u800c\u7522\u751f\u7684\u4e00\u7a2e\u77ed\u66ab\u7684\u81ea\u7531\u57fa(short-lived radical production)\u3002\u5b83\u662f\u6839\u64da\u74b0\u5883\u56e0\u7d20\uff08\u4f8b\u5982\u7d2b\u5916\u7dda\uff0c\u9999\u7159\u7159\u9727\uff0c\u74b0\u5883\u6c61\u67d3\u7269\u548cg\u5c04\u7dda\u8f3b\u5c04\uff09\u5f62\u6210\u7684\uff0c\u6216\u884d\u751f\u81ea\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6216NADPH\u6c27\u5316\u9176\u7b49\u6c27\u5316\u9176\u3002 O2\uff0d\u4e00\u65e6\u5f62\u6210\uff0c\u5c31\u6703\u653b\u64ca\u7d30\u80de\u6210\u5206\u4e26\u7834\u58de\u8102\u8cea\uff0c\u86cb\u767d\u8cea\u548cDNA\u3002\u9019\u6703\u5f15\u767c\u591a\u7a2e\u75be\u75c5\uff0c\u5305\u62ec\u764c\u75c7(diseases)\uff0c\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\uff0c\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\uff0c\u7cd6\u5c3f\u75c5(diabetes)\uff0c\u809d\u640d\u5bb3(liver damage)\u548c\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71\u75be\u75c5(central nervous system disorders)\u3002CheKine\u2122\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2(CheKine\u2122 Superoxide anion Scavenging Capacity Assay Kit<\/a>)\u70ba\u7814\u7a76\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u6db2\u548c\u5176\u4ed6\u751f\u7269\u6db2\u4e2d\u7684\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u80fd\u529b\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08xanthine oxidase , XO\uff09\u50ac\u5316\u53cd\u61c9\u63d0\u4f9b\u4e86\u8d85\u6c27\u9670\u96e2\u5b50\uff08superoxide anion , O2-\uff09\u3002 O2-\u8207\u56db\u5511\u9393\u9e7dWST-8\u67d3\u6599\u53cd\u61c9\u5f62\u6210\u6c34\u6eb6\u6027\u7684\u6709\u8272formazan\u7522\u7269\uff0c\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u9032\u884c\u6bd4\u8272\u5b9a\u91cf\u3002\u6a23\u54c1\u6e05\u9664\u4e86O2\uff0d\uff0c\u56e0\u6b64\u7528\u65bc\u986f\u8272\u53cd\u61c9\u7684O2\uff0d\u8f03\u5c11\u3002\u901a\u904e\u6bd4\u8272\u6cd5\u5728OD 450nm\u4e0b\u6e2c\u91cf\u6a23\u54c1\u7684\u9019\u7a2e\u6e05\u9664\u80fd\u529b\u3002<\/p>\n

\"HOT\"<\/a>CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0| \u00a0CheKine\u2122 Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit\u00a0\u8ca8\u865f\u00a0KTB1091<\/a><\/span><\/strong><\/p>\n

\u7fa5\u57fa\u81ea\u7531\u57fa(hydroxyl radical)\u2022OH\uff0c\u662f\u6c2b\u6c27\u6839\u96e2\u5b50\uff08hydroxide ion , OH-)\uff0c\u9ad8\u53cd\u61c9\u6027\uff08\u5bb9\u6613\u8b8a\u6210\u7fa5\u57fa\uff09\u3002\u7fa5\u57fa\u81ea\u7531\u57fa(Hydroxyl free radicals)\u4f5c\u7528\u65bc\u9ad4\u5167\u7684\u86cb\u767d\u8cea(proteins)\u3001\u6838\u9178(nucleic acids)\u3001\u8102\u985e(lipids)\u7b49\u751f\u7269\u5206\u5b50\uff0c\u5c0d\u7d30\u80de\u9020\u6210\u640d\u5bb3\u7d50\u69cb(structure)\u548c\u529f\u80fd(function)\uff0c\u9032\u800c\u5c0e\u81f4\u9ad4\u5167\u4ee3\u8b1d\u7d0a\u4e82(metabolic disorders)\u4e26\u5f15\u8d77\u75be\u75c5\u3002\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b(Hydroxyl free radical scavenging capacity)\u662f\u6a23\u54c1\u6297\u6c27\u5316\u80fd\u529b\u7684\u91cd\u8981\u6307\u6a19\u4e4b\u4e00\uff0c\u5df2\u5ee3\u6cdb\u61c9\u7528\u65bc\u6297\u6c27\u5316\u4fdd\u5065\u54c1(antioxidant health products)\u548c\u85e5\u7269(drugs)\u7684\u7814\u7a76\u3002 CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2(CheKine\u2122 Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit, KTB1091)\u5c08\u70ba\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b(Hydroxyl Free Radical Scavenging Capacity)\u800c\u958b\u767c\u3002\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u52a0\u9748\u654f\u6e96\u78ba\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cH2O2\/ Fe2+ \u7522\u751f\u7fa5\u57fa\u81ea\u7531\u57fa(hydroxyl free radical)\u901a\u904e\u82ac\u9813\u53cd\u61c9(Fenton reaction)\u3002\u6c34\u694a\u9178(Salicylic acid)\u80fd\u6709\u6548\u6355\u6349\u751f\u6210\u7684\u7fa5\u57fa\u81ea\u7531\u57fa(hydroxyl free radical)\u4e26\u8207\u4e4b\u53cd\u61c9\u751f\u6210\u7522\u751f\u6700\u5927\u5438\u6536\u5cf0\u5728 520 nm \u7684 2,3-\u4e8c\u7fa5\u57fa\u82ef\u7532\u9178(2,3-dihydroxybenzoic acid)\u3002\u5728\u5177\u6709\u80fd\u529b\u7684\u7269\u8cea\u4e4b\u5f8c\u6e05\u9664\u7fa5\u57fa\u81ea\u7531\u57fa\uff0c\u5c0e\u81f4520nm\u5438\u5149\u5ea6\u4e0b\u964d\u3002 520 nm \u7684\u5438\u5149\u5ea6\u503c\u53ef\u4ee5\u53cd\u6620\u6a23\u54c1\u7684\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u3002<\/p>\n

\"HOT\"<\/a><\/span><\/strong>CheKine\u2122 \u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Superoxide Anion Colorimetric Assay Kit\u8ca8\u865fKTB1210<\/a><\/span><\/strong><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n

absorption peak at 540nm\u00a0 0.0125-0.5 mg\/mL<\/p>\n

\u751f\u7269\u9ad4\u5167\u7684\u8d85\u6c27\u9670\u96e2\u5b50(Reactive oxygen species)\u7b49\u6d3b\u6027\u6c27\u5177\u6709\u514d\u75ab(immune)\u548c\u4fe1\u865f\u8f49\u5c0e\u4f5c\u7528(signal transduction effects)\uff0c\u4f46\u7576\u5b83\u5011\u7a4d\u7d2f\u904e\u591a\u6642\uff0c\u6703\u7834\u58de\u7d30\u80de\u819c(cell membranes)\u548c\u751f\u7269\u5927\u5206\u5b50(biological macromolecules)\uff0c\u5c0e\u81f4\u9ad4\u5167\u7d30\u80de\u548c\u7d44\u7e54\u7684\u4ee3\u8b1d\u7570\u5e38(abnormal metabolism)\uff0c\u5f15\u8d77\u591a\u7a2e\u75be\u75c5\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6aa2\u6e2c\u52d5\u7269\u7d44\u7e54\u3001\u690d\u7269\u7d44\u7e54\u3001\u8840\u6e05\u3001\u7d30\u80de\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u54c1\u4e2dO2-\u542b\u91cf\u7684\u7c21\u4fbf\u65b9\u6cd5\u3002\u6e2c\u5b9a\u4e2d\uff0c\u591a\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684\u8d85\u6c27\u9670\u96e2\u5b50(superoxide anion)\u8207\u9e7d\u9178\u7fa5\u80fa(hydroxylamine hydrochloride)\u53cd\u61c9\u751f\u6210NO2\uff0d\uff0cNO2\uff0d\u8207Gris\u8a66\u5291(Gris\u2019 reagent)\u53cd\u61c9\u3002 Gris\u5206\u6790\u7684\u6a5f\u7406\u6982\u62ec\u70ba\u91cd\u6c2e\u4e4b\u9593\u7684\u5076\u6c2e\u5076\u806f\uff0c\u5373\u78fa\u80fa\u985e(sulfonamides)\u8207NO2-\u548cN-(1-\u8418\u57fa)\u4e59\u4e8c\u80fa\u4e8c\u9e7d\u9178\u9e7d\u751f\u6210\u7d05\u8272\u5076\u6c2e\u5316\u5408\u7269\uff0c\u5728540nm\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002\u6a23\u54c1\u4e2d\u7684 O2- \u542b\u91cf\u53ef\u6839\u64da A540 \u503c\u8a08\u7b97\u3002<\/p>\n

\"HOT\"<\/a><\/span><\/strong>Coenzyme \u2160 NAD(H)\u00a0\u5206\u6790\u8a66\u5291\u76d2| Micro Coenzyme \u2160 NAD(H) Assay Kit\u8ca8\u865fKTB1020<\/a><\/span><\/p>\n

\u7159\u91af\u80fa\u817a\u82f7\u4e8c\u6838\u82f7\u9178(Nicotinamide adenine dinucleotide , NAD)\u662f\u4e00\u7a2e\u9176\u5171\u9ad4(enzymatic cofactor)\uff0c\u6d89\u53ca\u8a31\u591a\u6c27\u5316\u9084\u539f\u53cd\u61c9(redox reactions)\u3002NAD\u4f5c\u70ba\u96fb\u5b50\u8f09\u9ad4(redox reactions),\u5728\u6c27\u5316 (NAD) \u548c\u9084\u539f (NADH) \u5f62\u5f0f\u4e4b\u9593\u8ff4\u5708\u3002NAD \u9664\u4e86\u5728\u7d05\u6c27\u53cd\u61c9\u4e2d\u7684\u4f5c\u7528\u5916,\u5728 ADP-\u6838\u7cd6\u9178\u53cd\u61c9\u4e2d\u626e\u6f14\u91cd\u8981\u7684\u4f5c\u7528,\u4e26\u4e14\u4f5c\u70basirtuins\u7684substrate\u3002Nicotinamide adenine dinucleotide (NAD) is an enzymatic cofactor involved in many redox reactions. NAD functions as an electron carrier, cycling between the oxidized (NAD) and reduced (NADH) forms. In addition to its role in redox reactions, NAD plays critical roles in ADP-ribosylation reactions and as a substrate for sirtuins.<\/p>\n

\"\"<\/p>\n

Fig. Standard Curve of NAD in 96-well plate assay. The y-axis is \u0394OD and the x-axis is NAD concentration (uM).
\nAbbkine\u00a0Micro Coenzyme \u2160 NAD(H) Assay Kit(\u8ca8\u865fKTB1020), \u6aa2\u6e2c\u5404\u7a2e\u7d44\u7e54\u548c\u7d30\u80de\u5404\u7a2e\u80de\u5668\u4e2dNAD \u548c NADH\u6bd4\u4f8b\u3002\u6b64\u8a66\u5291\u76d2\u57fa\u65bc\u9176\u5faa\u74b0\u53cd\u61c9 (\u5b83\u4e0d\u8fa8\u8a8d NADP+\/NADPH),\u5176\u4e2d\u5f62\u6210\u7684 NADH \u6e1b\u5c11\u4e86 formazan (MTT) \u3002\u4ee5\u6ce2\u9577 OD565 nm \u4e0b\u6e2c\u91cf\uff0c\u5438\u6536\u5149\u7684\u5f37\u5ea6\u8207\u6a23\u54c1\u4e2d\u7684 NAD+ \/NADH \u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002 96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/p>\n

\"HOT\"<\/a><\/span><\/strong>CheKine\u2122\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u5206\u6790\u00a0|\u00a0<\/strong>CheKine\u2122 Total Antioxidant Capacity (TAC) Assay Kit\u8ca8\u865f<\/strong>KTB1500<\/a>\u00a0 \u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/p>\n

\"\"<\/p>\n

Fig. Standard Curve of Total Antioxidant Capacity (TAC) Assay. The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).<\/p>\n

CheKine\u2122\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\uff08Total Antioxidant Capacity , TAC\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u6613\u7528\u7684\u6bd4\u8272\u6e2c\u5b9a\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u8840\u6e05\uff0c\u8840\u6f3f\uff0c\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\uff0c\u5c3f\u6db2\uff0c\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u6db2\u548c\u5176\u4ed6\u751f\u7269\u6d41\u9ad4\u4e2d\u7684\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u3002 \u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cFe3 + -TPTZ\u88ab\u6297\u6c27\u5316\u5291\u9084\u539f\u70baFe2 + -TPTZ\u3002 \u9176\u50ac\u5316\u7684\u53cd\u61c9\u7522\u7269Fe2 + -TPTZ\u53ef\u4ee5\u5728\u6bd4\u8272\u8b80\u6578\u70ba593 nm\u7684\u689d\u4ef6\u4e0b\u9032\u884c\u6e2c\u91cf\u3002\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM\u3002<\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: serum, plasma, cell culture supernatants, urine, tissue\/ cell lysates and other biological fluids.<\/p>\n

\"HOT\"<\/a><\/span><\/strong>\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44CheKine\u2122 Plant Flavonoids Colorimetric Assay Kit | \u8ca8\u865fKTB1530<\/a><\/span><\/strong><\/span><\/p>\n

\u9ec3\u916e\u985e\u5316\u5408\u7269(Flavonoids)\u662f\u4e00\u985e\u591a\u82ef\u57fa\u5316\u5408\u7269(polyphenyl compounds)\uff0c\u662f\u690d\u7269\u7684\u6b21\u751f\u4ee3\u8b1d\u7522\u7269(secondary metabolites of plants)\u3002\u5b83\u5011\u5c0d\u4eba\u9ad4\u5177\u6709\u6297\u708e(anti-inflammatory)\u3001\u6297\u83cc(antibacterial)\u3001\u964d\u8840\u8102(hypolipidemic effects)\u3001\u6e05\u9664\u9ad4\u5167\u7fa5\u57fa\u81ea\u7531\u57fa(scavenging hydroxyl free radicals)\u3001\u9810\u9632\u764c\u75c7(preventing cancer)\u7b49\u4f5c\u7528\u3002\u69f2\u76ae\u7d20(Quercetin)\u662f\u4e00\u7a2e\u5178\u578b\u7684\u985e\u9ec3\u916e(flavonoid)\u3002<\/p>\n

\"Fig.<\/p>\n

\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6aa2\u6e2c\u690d\u7269\u7d44\u7e54(plant tissues)\u4e2d\u985e\u9ec3\u916e(flavonoid)\u542b\u91cf\u7684\u7c21\u55ae\u65b9\u6cd5\u3002\u5728\u9e7c\u6027\u4e9e\u785d\u9178\u9e7d\u6eb6\u6db2()alkaline nitrite solution\u4e2d\uff0c\u690d\u7269\u6a23\u54c1\u4e2d\u7684\u9ec3\u916e\u985e\u5316\u5408\u7269(flavonoids)\u548c\u92c1\u96e2\u5b50(aluminum ions)\u5f62\u6210\u7d05\u8272\u7d61\u5408\u7269(red complex)\uff0c\u5728502 nm\u8655\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002\u901a\u904e\u6e2c\u91cf\u6a23\u54c1\u63d0\u53d6\u7269\u5728 502 nm \u8655\u7684\u5438\u5149\u5ea6\uff0c\u53ef\u4ee5\u8a08\u7b97\u51fa\u6a23\u54c1\u4e2d\u9ec3\u916e\u985e\u5316\u5408\u7269(flavonoid)\u7684\u542b\u91cf\u3002<\/p>\n

\"HOT\"<\/a><\/span>\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44CheKine\u2122 Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit | \u8ca8\u865fKTB1140<\/strong><\/span><\/p>\n

\u591a\u915a\u6c27\u5316\u9176\uff08Polyphenol Oxidase , PPO\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269(microorganisms)\u548c\u57f9\u990a\u7d30\u80de(cultured cells)\u4e2d\u3002\u4f5c\u70ba\u4e00\u7a2e\u542b\u9285\u6c27\u5316\u9176(copper-containing oxidase)\uff0cPPO\u53ef\u4ee5\u901a\u904e\u6c27\u5316\u4e00\u5143\u915a (oxidizing monohydric phenols)\u548c\u4e8c\u5143\u915a (dihydric phenols) \u7522\u751f\u918c (quinones)\u800c\u5f15\u8d77\u8910\u8b8a(browning)\u3002\u9019\u7a2e\u9176\u9084\u8207\u679c\u852c\u52a0\u5de5(fruit and vegetable processing)\u3001\u8336\u8449\u54c1\u8cea(tea quality)\u548c\u7d44\u7e54\u57f9\u990a(tissue culture)\u5bc6\u5207\u76f8\u95dc\u3002PPO\u591a\u915a\u6c27\u5316\u9176\u8a66\u5291\u76d2\u70ba\u6aa2\u6e2c\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u3001\u7d44\u7e54(tissues)\u3001\u7d30\u80de(cells)\u3001\u690d\u7269(plants)\u548c\u7d30\u83cc(bacteria)\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684 PPO \u6d3b\u6027\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u591a\u915a\u6c27\u5316\u9176\uff08PPO\uff09\u50ac\u5316\u5152\u8336\u915a\u751f\u6210\u918c\uff0c\u5176\u5728525 nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0(absorption peak)\u3002\u5728 525 nm \u8655\u918c\u7684\u589e\u52a0\u7387\u53ef\u4ee5\u53cd\u6620 PPO \u7684\u6d3b\u6027\u3002<\/p>\n

\"HOT\"<\/a><\/span><\/strong>CheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Micro Hydrogen Peroxide (H2O2) Assay Kit\u8ca8\u865fKTB1041<\/a><\/span><\/strong><\/p>\n

\u904e\u6c27\u5316\u6c2b (Hydrogen Peroxide , H2O2) \u662f\u4e00\u7a2e\u6d3b\u6027\u6c27(reactive oxygen)\u4ee3\u8b1d\u526f\u7522\u7269\uff0c\u5b83\u65e2\u662f\u7d30\u80de\u5167\u4fe1\u865f(intracellular signaling messenger)\u50b3\u905e\u7684\u4fe1\u4f7f\uff0c\u4e5f\u662f\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u7684\u4f86\u6e90\u3002\u904e\u6c27\u5316\u6c2b(Hydrogen peroxide)\u901a\u904e\u591a\u7a2e\u6a5f\u5236\u5728\u7d30\u80de\u4e2d\u7522\u751f\uff0c\u4f8b\u5982\u7531\u4e2d\u6027\u7c92\u7d30\u80de(neutrophils)\u548c\u5de8\u566c\u7d30\u80de(macrophages)\uff08respiratory burst, \u547c\u5438\u7206\u767c\uff09\u7522\u751f NOX \u4ecb\u5c0e\u7684 ROS(NOX-mediated ROS)\uff0c\u6216\u901a\u904e\u7dda\u7c92\u9ad4\u547c\u5438\u904e\u7a0b\u4e2d\u96fb\u5b50\u6d29\u6f0f(electron leak)\u7522\u751f\u7684\u8d85\u6c27\u9670\u96e2\u5b50\u6b67\u5316\u9176(superoxide anions)\u7522\u751f\u3002\u901a\u904e NF-\u03baB \u548c\u5176\u4ed6\u56e0\u5b50\u767c\u63ee\u4f5c\u7528\uff0c\u6c2b\u904e\u6c27\u5316\u7269\u4ecb\u5c0e(hydroperoxide-mediated pathways)\u7684\u9014\u5f91\u8207\u54ee\u5598(asthma)\u3001\u708e\u75c7\u6027\u95dc\u7bc0\u708e(inflammatory arthritis)\u3001\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\u3001\u7cd6\u5c3f\u75c5\u6027\u8840\u7ba1\u75c5(diabetic vasculopathy)\u3001\u9aa8\u8cea\u758f\u9b06\u75c7(osteoporosis)\u3001\u795e\u7d93\u9000\u884c\u6027\u75be\u200b\u200b\u75c5(neurodegenerative diseases)\u3001\u5510\u6c0f\u7d9c\u5408\u75c7(Down\u2019s syndrome)\u548c\u514d\u75ab\u7cfb\u7d71\u75be\u75c5(immune system diseases)\u6709\u95dc\u3002CheKine\u2122 \u5fae\u91cf\u904e\u6c27\u5316\u6c2b\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Micro Hydrogen Peroxide Assay Kit, KTB1041)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u6613\u7528\u7684\u6bd4\u8272\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u7528\u65bc\u6e2c\u91cf\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\u3001\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u7269\u548c\u5176\u4ed6\u751f\u7269\u9ad4\u6db2\u4e2d\u7684\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cH2O2 \u5c07\u4e9e\u9435(ferrous)\u6c27\u5316\u70ba\u9435\u96e2\u5b50(ferric ion)\uff0c\u4e8c\u7532\u915a\u6a59(xylenol orange)\u4ee5\u9ad8\u9078\u64c7\u6027\u8207\u9435\u96e2\u5b50\u7d50\u5408\u5f62\u6210\u6709\u8272\uff08\u7d2b\u8272\uff09\u7522\u7269\uff0c\u53ef\u5728 OD 580 nm \u8655\u901a\u904e\u6bd4\u8272\u6cd5\u6e2c\u91cf\u3002\u6a23\u54c1\u4e2d\u5b58\u5728\u7684\u904e\u6c27\u5316\u6c2b\u904e\u6c27\u5316\u6c2b (Hydrogen Peroxide , H2O2)\u8207\u7372\u5f97\u7684\u4fe1\u865f\u6210\u6bd4\u4f8b\u3002<\/span><\/p>\n

\"HOT\"<\/a><\/span>\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2| \u8ca8\u865fKTB1080<\/strong><\/span><\/p>\n

CheKine\u2122 Superoxide anion Scavenging Capacity Assay Kit<\/p>\n

\u8d85\u6c27\u9670\u96e2\u5b50\uff08superoxide anion , O2-\uff09\u662f\u901a\u904e\u5c07\u96fb\u5b50\u52a0\u5230\u6c27\u4e2d\u800c\u7522\u751f\u7684\u4e00\u7a2e\u77ed\u66ab\u7684\u81ea\u7531\u57fa(short-lived radical production)\u3002\u5b83\u662f\u6839\u64da\u74b0\u5883\u56e0\u7d20\uff08\u4f8b\u5982\u7d2b\u5916\u7dda\uff0c\u9999\u7159\u7159\u9727\uff0c\u74b0\u5883\u6c61\u67d3\u7269\u548cg\u5c04\u7dda\u8f3b\u5c04\uff09\u5f62\u6210\u7684\uff0c\u6216\u884d\u751f\u81ea\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6216NADPH\u6c27\u5316\u9176\u7b49\u6c27\u5316\u9176\u3002 O2\uff0d\u4e00\u65e6\u5f62\u6210\uff0c\u5c31\u6703\u653b\u64ca\u7d30\u80de\u6210\u5206\u4e26\u7834\u58de\u8102\u8cea\uff0c\u86cb\u767d\u8cea\u548cDNA\u3002\u9019\u6703\u5f15\u767c\u591a\u7a2e\u75be\u75c5\uff0c\u5305\u62ec\u764c\u75c7(diseases)\uff0c\u52d5\u8108\u7ca5\u6a23\u786c\u5316(atherosclerosis)\uff0c\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\uff0c\u7cd6\u5c3f\u75c5(diabetes)\uff0c\u809d\u640d\u5bb3(liver damage)\u548c\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71\u75be\u75c5(central nervous system disorders)\u3002CheKine\u2122\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2(CheKine\u2122 Superoxide anion Scavenging Capacity Assay Kit<\/a>)\u70ba\u7814\u7a76\u7d44\u7e54\/\u7d30\u80de\u88c2\u89e3\u6db2\u548c\u5176\u4ed6\u751f\u7269\u6db2\u4e2d\u7684\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u80fd\u529b\uff0c\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u4fbf\u7684\u6bd4\u8272\u6e2c\u5b9a\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u9ec3\u560c\u5464\u6c27\u5316\u9176\uff08xanthine oxidase , XO\uff09\u50ac\u5316\u53cd\u61c9\u63d0\u4f9b\u4e86\u8d85\u6c27\u9670\u96e2\u5b50\uff08superoxide anion , O2-\uff09\u3002 O2-\u8207\u56db\u5511\u9393\u9e7dWST-8\u67d3\u6599\u53cd\u61c9\u5f62\u6210\u6c34\u6eb6\u6027\u7684\u6709\u8272formazan\u7522\u7269\uff0c\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u9032\u884c\u6bd4\u8272\u5b9a\u91cf\u3002\u6a23\u54c1\u6e05\u9664\u4e86O2\uff0d\uff0c\u56e0\u6b64\u7528\u65bc\u986f\u8272\u53cd\u61c9\u7684O2\uff0d\u8f03\u5c11\u3002\u901a\u904e\u6bd4\u8272\u6cd5\u5728OD 450nm\u4e0b\u6e2c\u91cf\u6a23\u54c1\u7684\u9019\u7a2e\u6e05\u9664\u80fd\u529b\u3002<\/p>\n

\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/strong><\/span><\/p>\n

Abbkine\u6240\u6709\u751f\u5316\u8a66\u5291\u76d2\u90fd\u53ef\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6aa2\u6e2c\u7684\uff0c\u9700\u78ba\u8a8d\u4e00\u500b\u5148\u6c7a\u689d\u4ef6\uff1a\u5177\u5099\u8207\u5206\u5149\u5149\u5ea6\u8a08\u5339\u914d\u7684\u5fae\u91cf\u6bd4\u8272\u76bf\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u5206\u985e<\/span><\/td>\n\u8ca8\u865f<\/span><\/td>\n\u54c1\u9805<\/span><\/td>\n\u5167\u5bb9<\/span><\/td>\n\u66f4\u591a\u8cc7\u6599<\/span><\/td>\n<\/tr>\n
\n

\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span><\/p>\n

<\u6297\u6c27\u5316\u7dad\u751f\u7d20\u7cfb\u7d71&\u5c0f\u5206\u5b50><\/span><\/td>\n

\u8ca8\u865fKTB1070<\/span><\/td>\nCheKine\u2122<\/strong>\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/strong><\/a><\/span><\/td>\n\n

CheKine\u2122 Xanthine Oxidase Assay Kit<\/span><\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/span><\/td>\n

\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1910<\/span><\/td>\nCheKine\u2122<\/span><\/strong>\u6d3b\u6027\u6c27\uff08ROS<\/strong>\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2<\/strong><\/span><\/a><\/span>
\n<\/strong><<\/strong>\u87a2\u5149\u6aa2\u6e2c<\/strong>><\/strong><\/span><\/span><\/td>\n		\n

\u6d3b\u6027\u6c27ROS\u6aa2\u6e2c<\/span><\/p>\n

DCFH-DA (10 mM)<\/span><\/td>\n

\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/span><\/td>\n\u4e00\u6c27\u5316\u6c2e\uff08NO<\/span><\/strong>\uff09\u6aa2\u6e2c<\/strong><\/span><\/a>\u00a0<\/strong><\/span><\/span><\/td>\n\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1040<\/span><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\/\u89f8\u9176<\/em>\u6d3b\u6027\u6aa2\u6e2c<\/a><\/span><\/strong><\/td>\n\u6e2c\u91cf\u7dda\u6027\u7bc4\u570d\u70ba 2-75 \u00b5M, \u6700\u4f4e\u6e2c\u91cf\u6d3b\u6027\u70ba2 U\/ml\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTB1500<\/a><\/span><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529b\u5206\u6790<\/a><\/strong><\/span><\/td>\n\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1530<\/a><\/strong><\/span><\/td>\n\n

\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44<\/span><\/a><\/strong><\/span><\/p>\n

<<\/strong>\u690d\u7269<\/strong>><\/strong><\/span><\/td>\n

\u5075\u6e2c\u7bc4\u570d:0.156-10 mg\/g<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1050<\/span><\/td>\nCheKine\u2122\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\nMDA\u8207\u786b\u4ee3\u5df4\u6bd4\u59a5\u9178\uff08thiobarbituric acid ,TBA\uff09\u53cd\u61c9\u751f\u6210MDA-TBA\u52a0\u5408\u7269\u5728OD 532 nm\u8655\u9032\u884c\u6bd4\u8272\u5b9a\u91cf\u30021-100 \u00b5M\/L<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1091<\/span><\/td>\n\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\nHydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit\u00a0<\/a><\/strong><\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1210<\/a><\/strong><\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\n\u00a0Superoxide Anion Colorimetric Assay Kit<\/a><\/strong><\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/span><\/td>\n\n

Coenzyme \u2160 NAD(H)\u00a0\u5206\u6790\u8a66\u5291\u76d2|<\/strong><\/span><\/a><\/span><\/p>\n

<\u7d44\u7e54\/\u7d30\u80de><\/span><\/td>\n

NAD\u662f\u4e00\u7a2e\u9176\u5171\u9ad4(enzymatic cofactor)\uff0c\u6d89\u53ca\u8a31\u591a\u6c27\u5316\u9084\u539f\u53cd\u61c9(redox reactions)<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1041<\/a><\/strong><\/span><\/td>\nCheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/a><\/strong><\/span><\/td>\n\u904e\u6c27\u5316\u6c2b (Hydrogen Peroxide , H2O2) \u662f\u4e00\u7a2e\u6d3b\u6027\u6c27(reactive oxygen)\u4ee3\u8b1d\u526f\u7522\u7269<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1080<\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2| <\/span><\/a><\/strong><\/span><\/td>\nO2-\u8207\u56db\u5511\u9393\u9e7dWST-8\u67d3\u6599\u53cd\u61c9\u5f62\u6210\u6c34\u6eb6\u6027\u7684\u6709\u8272formazan\u7522\u7269<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\n

\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span><\/p>\n

<\u6297\u6c27\u5316\u9175\u7d20\u7cfb\u7d71><\/span><\/td>\n

\u8ca8\u865fKTB1610<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/span><\/p>\n

\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/span><\/strong><\/a><\/span><\/td>\n

\u7a40\u80f1\u7518\u80bd\u6c27\u5316\uff08GSSG\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2\uff1a\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53ef\u8207DTNB\u53cd\u61c9\u4e26\u751f\u62102-\u785d\u57fa-5-\u5df0\u57fa\u82ef\u7532\u9178\uff0c\u5728412nm\u6ce2\u9577\u8655\u5177\u6709\u6700\u5927\u7684\u5149\u5438\u6536\u7387\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1600<\/span><\/td>\n\u82f1\u6587\u540d\u7a31: CheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit
\n\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\uff08GSH\uff09\u6aa2\u6e2c<\/a><\/strong><\/span><\/span><\/td>\n		\u5229\u7528DTNB\u8207\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53cd\u61c9\u5f62\u6210\u9ec3\u8272\u7522\u7269\u3002\u5728412 nm\u8655\u6e2c\u5f97\u7684\u5149\u5bc6\u5ea6\u53ef\u4ee5\u76f4\u63a5\u53cd\u6620\u6a23\u54c1\u4e2d\u7684\u7a40\u80f1\u7518\u80bd\u6fc3\u5ea6\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865f\u00a0KTB1620<\/span><\/td>\n\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit
\n\u6c27\u5316\u578b\u7a40\u80f1\u7518\u80bd\uff08GSSG\uff09\u6aa2\u6e2c<\/strong><\/a><\/span><\/span><\/td>\n		\u9084\u539f\u578b\u7a40\u80f1\u7518\u80bd\u53ef\u8207DTNB\u53cd\u61c9\u4e26\u751f\u62102-\u785d\u57fa-5-\u5df0\u57fa\u82ef\u7532\u9178\uff0c\u5728412nm\u6ce2\u9577\u8655\u5177\u6709\u6700\u5927\u7684\u5149\u5438\u6536\u7387\u300210-20uM<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1640<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/span><\/p>\n

GSH-Px \u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6aa2\u6e2c<\/span><\/strong><\/a><\/span><\/p>\n

 <\/td>\n

GSH-Px\u50ac\u5316H2O2\u6c27\u5316GSH\u751f\u6210GSSG\uff1b\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\uff08GR\uff09\u50ac\u5316NADPH\u9084\u539fGSSG\u5f9e\u800c\u518d\u751fGSH\uff0c\u800cNADPH\u6c27\u5316\u7522\u751fNADP +\u3002 NADPH\u5728340 nm\u8655\u6709\u4e00\u500b\u7279\u5fb5\u5438\u6536\u5cf0\uff0c\u800cNADP +\u6c92\u6709\u3002\u901a\u904e\u6e2c\u91cf340 nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4f86\u78ba\u5b9aNADPH\u812b\u6c2b\u901f\u7387\uff0c\u4ee5\u8a08\u7b97GSH-Px\u6d3b\u6027\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1630<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/span><\/p>\n

GST\u7a40\u80f1\u7518\u80bdS-\u8f49\u79fb\u9176\u5206\u6790<\/span><\/strong><\/a><\/span><\/td>\n

FGST\u50ac\u5316GSH\u8207CDNB\u7684\u7d50\u5408\u3002\u5171\u8edb\u4f34\u96a8\u8457\u5728340nm\u8655\u5438\u5149\u5ea6\u7684\u589e\u52a0\u3002\u589e\u52a0\u901f\u7387\u8207\u6a23\u54c1\u4e2d\u7684GST\u6d3b\u6027\u6210\u6b63\u6bd4\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1650<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/span><\/p>\n

\u00a0\u786b\u6c27\u9084\u86cb\u767d\u9084\u539f\u9176 (TrxR) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/span><\/strong><\/a><\/span><\/td>\n

TrxR\u53ef\u4ee5\u50ac\u5316NADPH\u9084\u539fDTNB\u5f9e\u800c\u751f\u6210TNB\u548cNADP +\u3002 TNB\u5728412nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0\u3002 TrxR\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf412 nm\u8655TNB\u7684\u589e\u52a0\u901f\u7387\u4f86\u8a08\u7b97\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1660<\/a><\/span><\/td>\n\n

\u82f1\u6587\u540d\u7a31:\u00a0CheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/span><\/p>\n

\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/strong><\/span><\/a><\/span><\/td>\n

TPX\u50ac\u5316H2O2\u6c27\u5316\u4e8c\u786b\u8607\u7cd6\u9187\uff08DTT\uff09\u3002 H2O2\u7684\u5438\u6536\u6ce2\u9577\u70ba240nm\u3002 TPX\u6d3b\u6027\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf240nm\u8655\u5438\u5149\u5ea6\u7684\u964d\u4f4e\u901f\u7387\u4e26\u6e1b\u53bb\u904e\u6c27\u5316\u6c2b\u9176\uff08CAT\uff09\u50ac\u5316\u7684H2O2\u4f86\u8a08\u7b97\u3002\u56e0\u6b64\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u4ee5\u540c\u6642\u6e2c\u91cf\u6a23\u54c1\u7684TPX\u548cCAT\u6d3b\u6027\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1030<\/span><\/td>\n\n

Superoxide dismutase Activity Assay Kit,<\/a><\/strong><\/span><\/p>\n

\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/strong><\/a><\/span><\/td>\n

WST-8\u6cd5\u6e2c\u5b9aSOD\u6d3b\u6027\uff0c\u6700\u5927\u6291\u5236\u7387\u53ef\u63a5\u8fd1100%\uff0c\u4e0d\u53d7\u4e00\u4e9b\u5e38\u898b\u5e72\u64fe\u56e0\u7d20\u7684\u5e72\u64fe\u3002\u5ee3\u6cdb\u7684\u7dda\u6027\u7bc4\u570d\uff1a3.13-200U\/mL\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fCEA660Ge<\/span><\/td>\n8-<\/u>\u7fa5\u57fa\u53bb\u6c27\u9ce5\u82f7<\/u>(8-OHdG)<\/u>\u6aa2\u6e2c\u8a66\u5291\u76d2<\/u>(<\/u>\u9175\u7d20\u514d\u75ab\u5206\u6790\u6cd5<\/u> <\/strong><\/span><\/td>\n\u5075\u6e2c\u7684\u9748\u654f\u5ea674.07-6000pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea627.53pg\/mL<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1011<\/strong><\/span><\/td>\nCheKine\u2122 \u8461\u8404\u7cd6-6-\u78f7\u9178\u812b\u6c2b\u9176 (G6PDH) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTB1011<\/span><\/strong><\/a><\/span><\/td>\n\u6a23\u54c1\u4e2d\u5b58\u5728\u7684 G6PDH \u5c07 NADP+ \u8f49\u5316\u70ba NADPH\uff0c\u5176\u5728 340 nm \u8655\u5177\u6709\u5438\u5149\u5ea6\u3002<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1140<\/a><\/strong><\/span><\/td>\n\n

PPO\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44<\/a><\/strong><\/span><\/p>\n

 <\/td>\n

Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit<\/a><\/strong><\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n
\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5<\/span>
\n <\u86cb\u767d\u8cea><\/span><\/td>\n		\u8ca8\u865fKTB120<\/a>0<\/strong><\/span><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fKTB120<\/span><\/a>0<\/span><\/strong><\/span><\/td>\n\u7fb0\u57fa\u86cb\u767d(Protein carbonyl)\u662f\u86cb\u767d\u8cea\u6c27\u5316\u4fee\u98fe(protein oxidative modification)\u904e\u7a0b\u4e2d\u65e9\u671f\u4fe1\u865f\uff0c\u662f\u8861\u91cf\u86cb\u767d\u8cea\u6c27\u5316\u640d\u50b7(protein oxidative damage)\u7684\u4e3b\u8981\u6307\u6a19<\/span><\/td>\n\u64cd\u4f5c\u624b\u518a<\/a><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010\u6297\u6c27\u5316\u6d3b\u6027\u5206\u6790 \u3011\u5e38\u898b\u6297\u6c27\u5316\u6d3b\u6027\u7684\u8a55\u4f30\u65b9\u6cd5 \u4eba\u9ad4\u5728\u6b63\u5e38\u4ee3\u8b1d\u904e\u7a0b\u4e2d\u6703\u7522\u751f\u81ea\u7531\u57fa\uff08free radical\uff09\u8207\u6d3b […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[5502],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39458"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=39458"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39458\/revisions"}],"predecessor-version":[{"id":39467,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/39458\/revisions\/39467"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=39458"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=39458"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=39458"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}