{"id":37389,"date":"2023-01-09T10:45:17","date_gmt":"2023-01-09T02:45:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37389"},"modified":"2025-10-26T12:22:11","modified_gmt":"2025-10-26T04:22:11","slug":"%e9%9d%9e%e6%b4%b2%e8%b1%ac%e7%98%9f%e7%97%85%e6%af%92%e8%9e%a2%e5%85%89pcr%e9%ab%98%e6%95%8f%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e5%bf%ab%e9%9d%88%e7%94%9f%e7%89%a9quicking-biotech","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=37389","title":{"rendered":"\u975e\u6d32\u8c6c\u761f\u75c5\u6bd2\u87a2\u5149PCR\u9ad8\u654f\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u5feb\u9748\u751f\u7269Quicking biotech"},"content":{"rendered":"

\u975e\u6d32\u8c6c\u761f\u75c5\u6bd2\u87a2\u5149PCR\u9ad8\u654f\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/strong><\/p>\n

\"\"Real-time PCR Diagnostic Kit for African Swine Fever<\/span><\/p>\n

\u3010\u4f5c\u7528\u8207\u7528\u9014\u3011\r\n\r\n<\/span><\/strong><\/span>\u7528\u65bc\u8c6c\u813e\u81df\u3001\u6dcb\u5df4\u7d50\u3001\u8089\u54c1\u3001\u8840\u6db2\u53ca\u8840\u6db2\u88fd\u54c1\u4e2d\u975e\u6d32\u8c6c\u761f\u75c5\u6bd2\u6838\u9178\u7684\u6aa2\u6e2c\u3002\r\n<\/span>\u9ad8\u654f\u8a66\u5291\u76d2\uff0c\u9867\u540d\u601d\u7fa9\u5373\u9ad8\u9748\u654f\u5ea6\u64f4\u589e\u8a66\u5291\u76d2\uff0c\u8a72\u8a66\u5291\u76d2\u6bd4\u5e38\u898f\u8a66\u5291\u76d2\u7684\u9748\u654f\u5ea6\u81f3\u5c11\u63d0\u9ad810\u500d\u4ee5\u4e0a\uff0c\u53ef\u6aa2\u6e2c\u4f4e\u65bc400copies\/ml\u6fc3\u5ea6\u7684\u6a23\u672c\uff0c\u53ef\u5728\u7b2c\u4e00\u6642\u9593\u6aa2\u6e2c\u5230\u6975\u4f4e\u6fc3\u5ea6\u7684\u75c5\u6bd2\uff0c\u5f9e\u800c\u8b93\u5ba2\u6236\u7b2c\u4e00\u6642\u9593\u63a1\u53d6\u63aa\u65bd\u3002\u9069\u7528\u5834\u666f\uff1a\u5404\u7d1a\u990a\u6b96\u5834\/\u5be6\u9a57\u5ba4\u975e\u761f\u9632\u63a7\u7684\u7cbe\u51c6\u7be9\u67e5\uff1b\u5f15\u7a2e\/\u4ea4\u6613\/\u6d41\u901a\u74b0\u7bc0\u7684\u7cbe\u51c6\u7be9\u67e5\u3002<\/pre>\n
 <\/p>\n
\"\"<\/p>\n
\u3010\u7528\u6cd5\u8207\u5224\u5b9a\u3011<\/strong><\/span>\r\n1 \u7528\u6cd5\r\n1.1 \u6a23\u54c1\u8655\u7406\u4e0b\u5217\u6a23\u54c1\u8655\u7406\u5f8c\u7f6e60\u2103\u6ec5\u6d3b30 \u5206\u9418\u3002\r\n1.1.1 \u7d44\u7e54\u6a23\u672c\u53d6100\uff5e200mg \u5f85\u6aa2\u7d44\u7e54\u6a23\u672c\uff08\u813e\u81df\u3001\u6dcb\u5df4\u7d50\u3001\u8089\u985e\uff09\uff0c\u7528\u7814\u7f3d\u5145\u5206\u7814\u78e8\u5f8c\uff0c\u52a0\u5165PBS\uff08pH\r\n\u503c7.2\uff091ml\uff0c\u6df7\u52fb\uff1b\u6216\u52a0\u51651ml PBS\uff08pH \u503c7.2\uff09\u53ca\u78c1\u73e0\u5f8c\u4ee5\u7d44\u7e54\u52fb\u6f3f\u5100\u52fb\u6f3f\u8655\u7406\u3002\u518d\u4ee510000r\/min \u96e2\u5fc3\r\n3 \u5206\u9418\uff0c\u5438\u53d6\u4e0a\u6e05200\u03bcl\uff0c\u7f6e\u65bc1.5ml \u96e2\u5fc3\u7ba1\u4e2d\uff0c\u7de8\u865f\u5099\u7528\u3002\r\n1.1.2 \u8840\u6db2\u63a1\u96c6\u6297\u51dd\u8840\uff0c\u5438\u53d6200\u03bcl\uff0c\u7f6e1.5ml \u96e2\u5fc3\u7ba1\u4e2d\uff0c\u7de8\u865f\u5099\u7528\u30021.1.3 \u8840\u6db2\u5236\u54c1\u53d6100\uff5e200mg \u5f85\u68c0\u8840\u6db2\u5236\u54c1\uff08\u5982\u8840\u6d46\u86cb\u767d\u7c89\uff09\uff0c\u7f6e\u4e8e1.5ml \u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u5165PBS\uff08pH\r\n\u503c7.2\uff091ml\uff0c\u5145\u5206\u6df7\u5300\u9707\u8361\u540e\uff0c\u4ee510000r\/min \u79bb\u5fc33 \u5206\u949f\uff0c\u5438\u53d6\u4e0a\u6e05200\u03bcl\uff0c\u7f6e\u4e8e1.5ml \u79bb\u5fc3\u7ba1\u4e2d\uff0c\u7f16\u53f7\u5907\r\n\u7528\u3002\r\n1.2 \u6837\u54c1\u5b58\u653e\u91c7\u96c6\u6216\u5904\u7406\u597d\u7684\u6837\u54c1\u57282\uff5e8\u2103\u4fdd\u5b58\u5e94\u4e0d\u8d85\u8fc724 \u5c0f\u65f6\uff1b\u82e5\u9700\u957f\u671f\u4fdd\u5b58\uff0c\u987b\u653e\u7f6e-70\u2103\u4ee5\u4e0b\r\n\u51b0\u7bb1\uff0c\u5e76\u5e94\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\r\n1.3 DNA \u63d0\u53d6\u7528\u5546\u54c1\u5316\u8bd5\u5242\u76d2\u6309\u5176\u8bf4\u660e\u4e66\uff0c\u5bf9\u5904\u7406\u597d\u7684\u6837\u54c1\u63d0\u53d6DNA\u3002\r\n1.4 \u8367\u5149PCR \u53cd\u5e94\u4f53\u7cfb\u7684\u914d\u5236\u53d6\u51fa\u8bd5\u5242\u76d2\u4e2d\u7684\u5404\u7ec4\u5206\uff0c\u7f6e\u5ba4\u6e29\uff0815\uff5e25\u2103\uff09\u878d\u5316\u540e\u77ac\u65f6\u79bb\u5fc3\uff0c\u5907\u7528\u3002\u6bcf\r\n\u6b21\u68c0\u9a8c\u8bbe\u9634\u3001\u9633\u6027\u5bf9\u7167\u3002\u8bbe\u5f85\u68c0\u6837\u54c1\u3001\u9633\u6027\u5bf9\u7167\u548c\u9634\u6027\u5bf9\u7167\u7684\u6837\u54c1\u603b\u4efd\u6570\u4e3an\uff0c\u6309\u5982\u4e0b\u53cd\u5e94\u4f53\u7cfb\u914d\u5236\uff1a\r\n\u5c07\u4ee5\u4e0a\u914d\u88fd\u7684\u53cd\u61c9\u7cfb\u7d71\u5145\u5206\u6df7\u5408\u5f8c\uff0c\u5206\u88dd\u81f3\u53cd\u61c9\u7ba1\u4e2d\uff0c\u6bcf\u7ba1 17\u03bcl\u3002\u5206\u5225\u53d6 DNA\u6a23\u672c 3\u03bcl \u52a0\u5165\u5c0d\u61c9\u53cd\u61c9\u7ba1\u4e2d\uff0c\u84cb\u7dca\u7ba1\u84cb\u5f8c\u77ac\u6642\u96e2\u5fc3\r\n<\/span><\/pre>\n
1.5 \u87a2\u5149 PCR \u53cd\u61c9\u689d\u4ef6\r\n\u7b2c\u4e00\u968e\u6bb5\uff1a95\u2103 20\u79d2\uff1b\r\n\u7b2c\u4e8c\u968e\u6bb5\uff1a95\u2103 1 \u79d2\u300160\u2103 20 \u79d2\uff0c\u5171 40 \u500b\u64f4\u589e\u5faa\u74b0\u3002\r\n\u5c0d\u65bc ABI\u87a2\u5149 PCR\u5100\uff0c\u9078\u64c7 FAM\u901a\u9053\u8b80\u53d6\u6aa2\u6e2c\u7d50\u679c\uff0c\u6dec\u6ec5\u57fa\u5718\u9078\u64c7\u201cMGB\u201d\r\n\r\n<\/span><\/pre>\n
\u5c0d\u65bc\u5176\u4ed6\u54c1\u724c\u7684\u87a2\u5149PCR \u5100\uff0c\u9078\u5b9aFAM \u901a\u9053\u8b80\u53d6\u6aa2\u6e2c\u7d50\u679c\uff0c\u6dec\u6ec5\u57fa\u5718\u9078\u64c7\u201cNone\u201d\u3002<\/p>\n
2 \u8a66\u9a57\u6210\u7acb\u689d\u4ef6\u967d\u6027\u5c0d\u7167Ct \u503c\uff1c35 \u4e26\u51fa\u73fe\u5178\u578b\u7684\u64f4\u589e\u66f2\u7dda\uff0c\u9670\u6027\u5c0d\u7167\u7121Ct \u503c\u4e26\u4e14\u7121\u7279\u7570\u6027\u64f4\u589e\u66f2\u7dda\uff0c<\/p>\n
\u5247\u5224\u70ba\u8a66\u9a57\u6210\u7acb\u3002<\/p>\n
3 \u5224\u5b9a\u5f85\u6aa2\u6a23\u54c1Ct \u503c\u226436 \u4e26\u51fa\u73fe\u5178\u578b\u7684\u64f4\u589e\u66f2\u7dda\uff0c\u5224\u70ba\u967d\u6027\uff1b\u5f85\u6aa2\u6a23\u54c1\u7121Ct \u503c\u4e26\u4e14\u7121\u7279\u7570\u6027\u64f4\u589e\u66f2<\/p>\n
\u7dda\uff0c\u5224\u70ba\u9670\u6027\uff1b36\uff1cCt \u503c\u226440 \u4e26\u51fa\u73fe\u5178\u578b\u7684\u64f4\u589e\u66f2\u7dda\uff0c\u5224\u70ba\u7591\u4f3c\u3002\u5c0d\u7591\u4f3c\u6a23\u54c1\uff0c\u7bc4\u672c\u91cf\u52a0\u500d\uff08\u53736\u03bcl DNA\uff09<\/p>\n
\u9032\u884c\u8907\u6aa2\uff08\u7121\u83cc\u7121\u6838\u9178\u9176\u6c34\u6e1b\u81f31.5\u03bcl\uff0c\u5176\u5b83\u9ad4\u7cfb\u4e0d\u8b8a\uff09\uff0cCt \u503c\u226440 \u4e26\u51fa\u73fe\u5178\u578b\u7684\u64f4\u589e\u66f2\u7dda\u5224\u70ba\u967d\u6027\uff0c\u7121Ct<\/p>\n
\u503c\u4e26\u4e14\u7121\u7279\u7570\u6027\u64f4\u589e\u66f2\u7dda\u5247\u5224\u70ba\u9670\u6027\u3002<\/p>\n
\u3010\u6ce8\u610f\u4e8b\u9805\u3011<\/strong><\/span><\/p>\n
\uff081\uff09\u8a66\u5291\u76d2\u61c9\u5728-20\u2103\u4ee5\u4e0b\u4fdd\u5b58\u548c\u904b\u8f38\u3002<\/p>\n
\uff082\uff09\u672c\u8a66\u5291\u76d2\u50c5\u7528\u65bc\u9ad4\u5916\u6aa2\u6e2c\u4f7f\u7528\uff0c\u64cd\u4f5c\u4eba\u54e1\u5fc5\u9808\u7d93\u904e\u57f9\u8a13\uff0c\u8a66\u5291\u76d2\u4f7f\u7528\u524d\u8acb\u4ed4\u7d30\u95b1\u8b80\u8aaa\u660e\u66f8\u5168\u6587\u3002<\/p>\n
\uff083\uff09\u4e0d\u8981\u4f7f\u7528\u904e\u671f\u7d44\u5206\u6216\u8005\u4e0d\u540c\u6279\u6b21\u7d44\u5206\u6df7\u5408\u4f7f\u7528\u3002<\/p>\n
\uff084\uff09\u4f7f\u7528\u6642\u5118\u91cf\u907f\u514d\u53cd\u5fa9\u51cd\u878d\uff0c\u5982\u51cd\u878d\uff0c\u61c9\u4e0d\u8d85\u904e3 \u6b21\u3002<\/p>\n
\uff085\uff09\u6a23\u54c1\u63a1\u96c6\u548c\u8655\u7406\u904e\u7a0b\u61c9\u6234\u4e00\u6b21\u6027\u624b\u5957\uff0c\u4e26\u53ca\u6642\u66f4\u63db\uff0c\u6a23\u54c1\u9593\u56b4\u7981\u4ea4\u53c9\u6c61\u67d3\u3002<\/p>\n
\uff086\uff09\u87a2\u5149PCR \u5be6\u9a57\u56b4\u683c\u5206\u5340\u64cd\u4f5c\uff0c\u5404\u5340\u61c9\u6709\u5c08\u7528\u7684\u624b\u5957\u3001\u79fb\u6db2\u5668\u7b49\uff0c\u4e0d\u5f97\u4ea4\u53c9\u4f7f\u7528\uff0c\u907f\u514d\u6c61\u67d3\uff1b\u5de5\u4f5c\u4eba\u54e1<\/p>\n
\u61c9\u9075\u5faa\u55ae\u65b9\u5411\u5de5\u4f5c\u539f\u5247\uff0c\u5404\u5de5\u4f5c\u5340\u76f8\u5c0d\u9694\u96e2\u3002<\/p>\n
\uff087\uff09\u9032\u884c\u87a2\u5149PCR \u5be6\u9a57\u7684\u5de5\u4f5c\u684c\u9762\u53ca\u76f8\u95dc\u7269\u54c1\u61c9\u5b9a\u671f\u75281%\u6b21\u6c2f\u9178\u9209\u300175%\u9152\u7cbe\u30011mol\/L \u9e7d\u9178\u4f9d\u6b21\u6ec5\u83cc\u548c\u6d88<\/p>\n
\u6bd2\uff0c\u6216\u5b9a\u671f\u7528\u7d2b\u5916\u71c8\u6ec5\u83cc\u548c\u6d88\u6bd2\u3002<\/p>\n
\uff088\uff09\u6e2c\u8a66\u6a23\u54c1\u6642\uff0c\u61c9\u505a\u597d\u751f\u7269\u5b89\u5168\u9632\u8b77\u3002<\/p>\n
\uff089\uff09\u8a66\u9a57\u5b8c\u7562\u5f8c\uff0c\u4f7f\u7528\u904e\u7684\u8a66\u5291\u76d2\u3001\u6a23\u54c1\u548c\u8a66\u5291\u61c9\u9032\u884c\u7121\u5bb3\u5316\u8655\u7406\u3002<\/p>\n
\u3010\u898f\u683c\u3011\uff081\uff0910 \u4efd\/\u76d2\uff082\uff0920 \u4efd\/\u76d2\uff083\uff0950 \u4efd\/\u76d2<\/p>\n
\u00a0<\/span><\/pre>\n
\u3002\r\n<\/span><\/pre>\n","protected":false},"excerpt":{"rendered":"
\u975e\u6d32\u8c6c\u761f\u75c5\u6bd2\u87a2\u5149PCR\u9ad8\u654f\u6aa2\u6e2c\u8a66\u5291\u76d2 Real-time PCR Diagnostic Kit for Afr […]<\/p>\n","protected":false},"author":3,"featured_media":37396,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5160],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37389"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37389"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37389\/revisions"}],"predecessor-version":[{"id":53165,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37389\/revisions\/53165"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37396"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37389"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37389"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}