{"id":36951,"date":"2022-12-08T13:56:35","date_gmt":"2022-12-08T05:56:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=36951"},"modified":"2024-10-02T10:37:35","modified_gmt":"2024-10-02T02:37:35","slug":"5x-sds-page-loading-buffer-5%e5%80%8d%e8%9b%8b%e7%99%bd%e8%b3%aa%e9%9b%bb%e6%b3%b3%e6%a8%a3%e6%9c%ac%e6%b6%b2%e8%b2%a8%e8%99%9fktd3003","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=36951","title":{"rendered":"5X SDS-PAGE Loading Buffer | 5\u500d\u86cb\u767d\u8cea\u96fb\u6cf3\u6a23\u672c\u6db2\u8ca8\u865fKTD3003"},"content":{"rendered":"

5X SDS-PAGE Loading Buffer \u00a0| 5<\/strong>\u500d<\/strong>\u86cb\u767d\u8cea\u96fb\u6cf3\u6a23\u672c\u6db2\u8ca8\u865f<\/strong>KTD3003<\/a>\u00a010ml\u00a0<\/span><\/p>\n

\"LINE\u7fa4\u767c<\/p>\n

SDS-PAGE Protein Sample Loading Buffer (5X)<\/p>\n

SDS-PAGE Protein Sample Loading Buffer (5\u00d7) \u662f\u4e00\u7a2e\u6539\u826f\u7684 5 \u500d\u6fc3\u7e2e\u86cb\u767d\u4e0a\u6a23\u7de9\u885d\u6db2(modified 5-fold concentrated protein loading buffer)\uff0c\u4ee5\u6eb4\u915a\u85cd(bromophenol blue)\u70ba\u67d3\u6599\u3002\u672c\u4e0a\u6a23\u7de9\u885d\u6db2\u4f7f\u7528\u7a69\u5b9a\u6027\u66f4\u597d\u3001\u9084\u539f\u6548\u679c\u66f4\u597d\u3001\u7121\u96e3\u805e\u6c23\u5473\u7684\u9084\u539f\u5291(reducing agent)\u4ee3\u66ff\u50b3\u7d71\u7684DTT\u62162-ME(2-mercaptoethanol)\u3002\u7528\u65bc\u5e38\u898f\u9084\u539fSDS-PAGE\u86cb\u767d\u6a23\u54c1\u7684\u4e0a\u6a23\u3002<\/p>\n

\"Fig<\/p>\n

Fig 1. The SDS-PAGE image of the protein extracted from the cell sample using ExKine\u2122 Pro Total Protein Extraction Kit for Animal Cultured Cells\/Tissues(Cat. No: KTP3007) and adding SDS-PAGE Protein Sample Loading Buffer (5X) (Cat. No: KTD3003); The left lane uses Abbkine products, and the remaining lanes are products of other brands.<\/p>\n

\u6aa2\u6e2c\u6b65\u9a5f<\/p>\n

Assay Procedure 1. SDS-PAGE Protein Sample Loading Buffer (5\u00d7) was dissolved at room temperature or no more than 37\u2103 water bath, then was stored immediately at room temperature to avoid being placed in the water bath for a long time. 2. According to the ratio of adding 1 \u00b5L SDS-PAGE Protein Sample Loading Buffer (5\u00d7) to every 4 \u00b5L protein sample, mix the protein sample and SDS-PAGE Protein Sample Loading Buffer (5\u00d7). 3. Heat at boiling water bath for 5-10 min to fully denature the protein. After cooling to room temperature, load the sample directly into the sample hole of the SDS-PAGE gel. 4. Electrophoresis is usually stopped until the blue dye reaches near the bottom end of the gel.<\/p>\n

1. SDS-PAGE\u86cb\u767d\u6a23\u54c1\u4e0a\u6a23\u7de9\u885d\u6db2\uff085\u00d7\uff09\u5728\u5ba4\u6eab\u6216\u4e0d\u8d85\u904e37\u2103\u6c34\u6d74\u4e2d\u6eb6\u89e3\uff0c\u7136\u5f8c\u7acb\u5373\u5728\u5ba4\u6eab\u4e0b\u4fdd\u5b58\uff0c\u907f\u514d\u9577\u6642\u9593\u653e\u7f6e\u5728\u6c34\u6d74\u4e2d\u3002<\/p>\n

2. \u4f9d\u7167\u6bcf 4 \u00b5L \u86cb\u767d\u8cea\u6a23\u672c\u52a0\u5165 1 \u00b5L SDS-PAGE Protein Sample Loading Buffer (5\u00d7) \u7684\u6bd4\u4f8b\uff0c\u5c07\u86cb\u767d\u8cea\u6a23\u672c\u8207 SDS-PAGE Protein Sample Loading Buffer (5\u00d7) \u6df7\u5408\u3002<\/p>\n

3. \u6cb8\u6c34\u6d74\u52a0\u71b15-10\u5206\u9418\uff0c\u4f7f\u86cb\u767d\u8cea\u5145\u5206\u8b8a\u6027\u3002\u51b7\u537b\u81f3\u5ba4\u6eab\u5f8c\uff0c\u5c07\u6a23\u54c1\u76f4\u63a5\u88dd\u5165 SDS-PAGE \u51dd\u81a0\u7684\u52a0\u6a23\u5b54\u4e2d\u3002<\/p>\n

4. \u901a\u5e38\u505c\u6b62\u96fb\u6cf3\u76f4\u81f3\u85cd\u8272\u67d3\u6599\u5230\u9054\u51dd\u81a0\u5e95\u7aef\u9644\u8fd1\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Kit components<\/strong><\/td>\n\u2022 SDS-PAGE Protein Sample Loading Buffer (5\u00d7)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Using a reducing agent with better stability, better reduction effect;
\n\u2022 No unpleasant odor;
\n\u2022 Instead of traditional DTT or 2-ME.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStored at -20\u2103 for 12 months.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Product name<\/strong><\/td>\nSDS-PAGE Protein Sample Loading Buffer (5X)<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine SDS-PAGE protein loading buffer (5\u00d7) is used for the loading of conventional reducing SDS-PAGE protein samples.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nSDS-PAGE Protein Sample Loading Buffer (5\u00d7) is a modified 5-fold concentrated protein loading buffer with bromophenol blue as the dye. This loading buffer uses a reducing agent with better stability, better reduction effect, and no unpleasant odor, instead of traditional DTT or 2-ME. It is used for the loading of conventional reducing SDS-PAGE protein samples.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"Untitled-1\"\u00a0<\/strong>\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

5X SDS-PAGE Loading Buffer \u00a0| 5\u500d\u86cb\u767d\u8cea\u96fb\u6cf3\u6a23\u672c\u6db2\u8ca8\u865fKTD3003\u00a010ml\u00a0 […]<\/p>\n","protected":false},"author":3,"featured_media":36952,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[135],"tags":[5089],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/36951"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=36951"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/36951\/revisions"}],"predecessor-version":[{"id":50338,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/36951\/revisions\/50338"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/36952"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=36951"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=36951"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=36951"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}