{"id":34902,"date":"2022-10-18T11:28:36","date_gmt":"2022-10-18T03:28:36","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=34902"},"modified":"2025-11-15T16:26:43","modified_gmt":"2025-11-15T08:26:43","slug":"%e8%9b%8b%e7%99%bd%e9%85%b6-k-proteinase-k-cas39450-01-6-apexbio-%e8%b2%a8%e8%99%9fk1037","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=34902","title":{"rendered":"\u86cb\u767d\u9176 K (Proteinase K) | CAS#39450-01-6 APExBIO \u8ca8\u865fK1037"},"content":{"rendered":"

Ser\/Thr \u86cb\u767d\u9176\u7cfb\u5217\u5546\u54c1->>\u86cb\u767d\u9176 K (Proteinase K)<\/span><\/span><\/span><\/span><\/strong><\/p>\n

APExBIO Technology LLC \u662f\u4e00\u5bb6\u9818\u5148\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5291 (Small Molecule Inhibitors)\/\u6d3b\u5316\u5291(Activators)\u3001\u5316\u5408\u7269\u5eab( Compound Libraries)\u3001\u80dc\u80bd(Peptides)\u3001\u6aa2\u6e2c\u8a66\u5291\u76d2(Assay Kits)\u3001\u87a2\u5149\u6a19\u8a18(Fluorescent Label)\u3001\u9176(Enzymes)\u3001\u4fee\u98fe\u6838\u82f7\u9178(Modified Nucleotides)\u3001mRNA \u5408\u6210\u4ee5\u53ca\u5404\u7a2e\u5206\u5b50\u751f\u7269\u5b78\u5de5\u5177\u7684\u4f9b\u61c9\u5546\u3002APExBIO\u63d0\u4f9b\u5ee3\u6cdb\u7684\u7522\u54c1\u7dda\uff0c\u6db5\u84cb 20 \u591a\u500b\u4e0d\u540c\u7684\u7814\u7a76\u9818\u57df\uff0c\u4f8b\u5982\u764c\u75c7(cancer)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neurosciences)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u548c\u8868\u89c0\u907a\u50b3\u5b78(epigenetics)\u7b49\u3002\u516c\u53f8\u7e3d\u90e8\u4f4d\u65bc\u7f8e\u570b\u5fb7\u5dde\u4f11\u58eb\u9813USA (Houston, Texas)\uff0c\u81f4\u529b\u65bc\u670d\u52d9\u5168\u7403\u5ba2\u6236\u3002\u9ad8\u5ea6\u91cd\u8996\u7522\u54c1\u54c1\u8cea\u3002\u6240\u6709\u7522\u54c1\u5747\u9075\u5faa\u56b4\u683c\u7684\u751f\u7522\u6307\u5357\uff0c\u4e26\u9644\u6709\u5206\u6790\u8b49\u66f8\u3001HPLC\u3001\u8cea\u8b5c(Mass Spectrum)\u548cHMNR\u4ee5\u53ca\u9ad4\u5916\u9a57\u8b49(in vitro validation)\u3002 APExBIO\u7522\u54c1\u5df2\u88ab\u300aNature\u300b\u3001\u300aCell\u300b\u548c\u300aScience\u300b\u7b49\u773e\u591a\u9802\u7d1a\u540c\u884c\u8a55\u5be9\u671f\u520a\u5f15\u7528\u3002>>\u66f4\u591aAPExBio\u5546\u54c1<\/span><\/strong><\/em><\/span><\/a><\/p>\n

<\/p>\n

\u86cb\u767d\u9176 K (Proteinase K) | CAS#39450-01-6 APExBIO\u00a0 \u8ca8\u865fK1037<\/a><\/span><\/p>\n

\u86cb\u767d\u9176 K (Proteinase K)\u662f\u4e00\u7a2e\u5ee3\u8b5c(broad spectrum) \u7d72\u6c28\u9178\u86cb\u767d\u9176(serine protease)\uff0c\u5728 DNA \u63d0\u53d6\u65b9\u6848\u4e2d\u7528\u65bc\u6d88\u5316\u6c61\u67d3\u86cb\u767d\u8cea(contaminating proteins)\uff0c\u5c24\u5176\u662f DNA \u9176(DNases)\u3002\u8a72\u9176\u88c2\u89e3\u86cb\u767d\u8cea\u4e2d\u8207\u8102\u80aa\u65cf(aliphatic)\u548c\u82b3\u9999\u65cf\u6c28\u57fa\u9178\u6b98\u57fa(aromatic amino acid residues)\u7684\u7fa7\u57fa\u76f8\u9130\u7684\u80bd\u9375(peptide bonds)\u3002\u86cb\u767d\u9176 K(Proteinase K) \u7684\u4e3b\u8981\u61c9\u7528\u662f\u5728 DNA \u548c RNA \u63d0\u53d6(RNA extraction)\u4e2d\u7684\u61c9\u7528\u3002\u5728\u5927\u591a\u6578\u6838\u9178\u63d0\u53d6 (nucleic acid extraction)\u65b9\u6848\u4e2d\uff0c\u86cb\u767d\u9176 K (Proteinase K)\u88ab\u6dfb\u52a0\u5230\u7d44\u7e54(tissue)\u6216\u88c2\u89e3\u7269(the lysate)\u4e2d\uff0c\u4ee5\u78ba\u4fdd\u5728\u6838\u9178\u63d0\u53d6\u904e\u7a0b\u4e2d\u5b8c\u5168\u6d88\u5316\u86cb\u767d\u8cea\uff0c\u5c24\u5176\u662f DNase \u548c RNase\u3002\u6d88\u5316\u86cb\u767d\u8cea(digest proteins)\u7684\u80fd\u529b\u4e5f\u4f7f\u86cb\u767d\u9176 K \u53ef\u7528\u65bc\u88fd\u5099\u7528\u65bc\u8108\u885d\u5834\u51dd\u81a0\u96fb\u6cf3 (pulsed-field gel electrophoresis)\u3001\u86cb\u767d\u8cea\u6307\u7d0b(protein fingerprinting)\u3001\u86cb\u767d\u9176\u8db3\u5370(protease foot printing)\u3001\u7dda\u7c92\u9ad4\u5206\u96e2(mitochondrial isolation)\u3001\u57fa\u56e0\u7d44 DNA (genomic DNA isolation)\u5206\u96e2\u7b49\u7684\u67d3\u8272\u9ad4 DNA(chromosomal DNA)\u3002\u86cb\u767d\u9176K(Proteinase K) \u7522\u54c1\u662f\u5f9e\u7562\u8d64\u9175\u6bcd\u7d30\u80de(Pichia pastoris cells)\u4e2d\u63d0\u53d6\u7684\uff0c\u514b\u9686\u4e86\u7de8\u78bcEngyodontium album (Tritirachium album) \u5167\u6eb6\u86cb\u767d\u9176\u7684\u57fa\u56e0\u3002\u5b83\u662f\u4e00\u7a2e\u9ad8\u6d3b\u6027\u86cb\u767d\u9176\uff0c\u5e38\u7528\u65bc\u6d88\u5316\u5404\u7a2e\u86cb\u767d\u8cea\u548c\u9176\uff08\u5305\u62ec\u6838\u9178\u5167\u5207\u9176\u3001\u6838\u9178\u5916\u5207\u9176\u3001DNase \u6216 RNase\uff09\u3002\u56e0\u6b64\uff0c\u5b83\u901a\u5e38\u7528\u65bc DNA \u88fd\u5099\u800c\u4e0d\u640d\u5bb3\u5206\u96e2 DNA \u7684\u5b8c\u6574\u6027(integrity)\u3002\u5b83\u5728\u5ee3\u6cdb\u7684\u689d\u4ef6\u4e0b\u5177\u6709\u5353\u8d8a\u7684\u6027\u80fd\uff1apH\u3001\u7de9\u885d\u6db2(buffer)\u3001\u53bb\u6c61\u5291\uff08\u5982 SDS\uff09\u3001\u87af\u5408\u5291\uff08\u5982 EDTA\uff09\u548c\u6eab\u5ea6(temperature)\u3002\u86cb\u767d\u9176 K \u512a\u5148\u6c34\u89e3\u8207\u758f\u6c34\u6c28\u57fa\u9178\uff0c\u8102\u80aa\u65cf(aliphatic)\u3001\u82b3\u9999\u65cf\u6c28\u57fa\u9178\u6b98\u57fa(aromatic amino acid residues)\u7684\u7fa7\u57fa\u76f8\u9130\u7684\u80bd\u9375\u3002<\/p>\n

\u9664\u4e86\u57fa\u56e0\u7d44(genome)\u7684\u5206\u96e2\u5916\uff0c\u5b83\u9084\u53ef\u4ee5\u7528\u65bc\u6aa2\u6e2c\u9176\u5b9a\u4f4d(localization)\u6216\u5f9eDNA\u4e2d\u53bb\u9664\u9176\u4ee5\u63d0\u9ad8\u514b\u9686\u6548\u7387(cloning efficiency)\u3002\u86cb\u767d\u9176 K (proteinase K)\u7684\u5408\u9069\u5de5\u4f5c\u6fc3\u5ea6\u59cb\u7d42\u5728 0.05 \u81f3 1 mg\/mL \u7684\u7bc4\u570d\u5167\u3002\u6dfb\u52a0\u7684\u86cb\u767d\u9176 K \u7684\u6fc3\u5ea6\u53d6\u6c7a\u65bc\u88ab\u6d88\u5316\u7684\u7d44\u7e54\u3002\u5c0d\u65bc\u5927\u591a\u6578\u7d44\u7e54\u4f86\u8aaa\uff0c200 \u00b5g\/ml \u7684\u5de5\u4f5c\u6fc3\u5ea6\u5c31\u8db3\u5920\u4e86\u3002\u6700\u7d42\u7528\u6236\u9700\u8981\u91dd\u5c0d\u8a72\u6a23\u54c1\u985e\u578b\u512a\u5316\u86cb\u767d\u9176 K \u7684\u6700\u4f73\u5de5\u4f5c\u6fc3\u5ea6\u3002\u9176\u7684\u6d3b\u6027\u53ef\u4ee5\u88ab 0.2 \u5230 1% SDS \u6216 1-4 mol \u5c3f\u7d20\u523a\u6fc0\u3002\u5b83\u88ab\u9223\uff081-5mM\uff09\u6fc0\u6d3b\uff0c\u96d6\u7136\u9223\u96e2\u5b50\u4e0d\u5f71\u97ff\u9176\u7684\u6d3b\u6027\uff0c\u4f46\u5b83\u6709\u52a9\u65bc\u71b1\u7a69\u5b9a\u6027\u4e26\u4fdd\u8b77\u86cb\u767d\u9176\u514d\u65bc\u81ea\u6eb6(autolysis)\u3002\u86cb\u767d\u9176 K(proteinase K) \u6709\u5169\u500b Ca2+ \u7d50\u5408\u4f4d\u9ede(substrate binding site)\uff0c\u9760\u8fd1\u6d3b\u6027\u4e2d\u5fc3\uff0c\u4f46\u4e0d\u76f4\u63a5\u53c3\u8207\u50ac\u5316\u6a5f\u5236\u3002\u56e0\u6b64\u9223\u96e2\u5b50\u5c0d\u86cb\u767d\u9176K\u7684\u5e95\u7269\u7d50\u5408\u4f4d\u9ede\u5177\u6709\u8abf\u7bc0\u4f5c\u7528\u3002\u8a72\u9176\u88abDIFP\u6216PMSF\u6ec5\u6d3b\u3002\u7136\u800c\uff0c\u5b83\u4e0d\u53d7 EDTA\u3001\u7898\u4e59\u9178(iodoacetic acid)\u3001\u80f0\u86cb\u767d\u9176\u7279\u7570\u6027\u6291\u88fd\u5291(trypsin-specific inhibitor) TLCK\u3001\u80f0\u51dd\u4e73\u86cb\u767d\u9176\u7279\u7570\u6027\u6291\u5236\u5291 TPCK(trypsin-specific inhibitor, TPCK) \u548c\u5c0d\u6c2f\u6c5e\u82ef\u7532\u9178\u9e7d(p-chloromercuribenzoate)\u7684\u6291\u5236\u3002\u5efa\u8b70\u6700\u4f73 pH \u503c\u70ba 7.5 \u81f3 8.0\uff0c\u6700\u4f73\u6eab\u5ea6\u70ba 50 \u81f3 55\u00b0C\u3002\u6eab\u5ea6\u9ad8\u65bc 65\u00b0C \u6642\u6703\u767c\u751f\u5feb\u901f\u8b8a\u6027\u3002\u53ef\u4ee5\u5c07\u5176\u5728 95\u00b0C \u4e0b\u4fdd\u6301 10 \u5206\u9418\u4f5c\u70ba\u71b1\u6ec5\u6d3b(heat inactivation)\u3002<\/p>\n\n\n\n\n\n\n\n\n\n
Cas No.<\/td>\n39450-01-6<\/td>\nSDF<\/td>\n<\/td>\n<\/tr>\n
Concentration<\/td>\n>600 U\/mL (approximately 20 mg\/mL)<\/td>\n<\/tr>\n
Formula<\/td>\n<\/td>\nM.Wt<\/td>\n29.3 kDa<\/td>\n<\/tr>\n
Solubility<\/td>\nSoluble in 20 mM Tris-HCl, 1 mM CaCl2<\/sub>, 50% Glycerol, pH 7.4<\/td>\nStorage<\/td>\nStore at -20\u00b0C<\/td>\n<\/tr>\n
Stability<\/td>\npH range:4.0 to 12.5, temperature range: 25\u00b0C to 65\u00b0C.<\/td>\n<\/tr>\n
General tips<\/td>\nApexbio guarantees optimal performance of this product for 18 months after date of delivery under the appropriate temperature and condition.<\/td>\n<\/tr>\n
Shipping Condition<\/td>\nEvaluation sample solution: ship with blue ice<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Enzyme activity: \u2265 30 units\/mg of protein<\/p>\n

Specific activity: \u2265 40 units\/mg of protein<\/p>\n

Purity: \u226595%\uff08Native-PAGE )<\/p>\n

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Product Citation<\/span><\/h2>\n<\/div>\n
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