{"id":34449,"date":"2022-09-28T13:09:08","date_gmt":"2022-09-28T05:09:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=34449"},"modified":"2025-03-10T11:58:38","modified_gmt":"2025-03-10T03:58:38","slug":"na%e2%81%bak%e2%81%ba-atp%e9%85%b6%e6%b4%bb%e6%80%a7%e5%88%86%e6%9e%90%e6%aa%a2%e6%b8%ac-chekine-micro-na%e2%81%bak%e2%81%ba-atpase-activity-assay-kit-%e8%b2%a8%e8%99%9f-ktb1800","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=34449","title":{"rendered":"Na\u207a\/K\u207a-ATP\u9176\u6d3b\u6027\u5206\u6790\u6aa2\u6e2c | CheKine\u2122 Micro Na\u207a\/K\u207a-ATPase Activity Assay Kit \u8ca8\u865f KTB1800"},"content":{"rendered":"

Na\u207a\/K\u207a-ATP\u9176\u6d3b\u6027\u5206\u6790\u6aa2\u6e2c | CheKine\u2122 Micro Na\u207a\/K\u207a-ATPase Activity Assay Kit \u8ca8\u865f KTB1800<\/span><\/strong><\/span><\/a><\/span><\/p>\n

Na\u207a\/K\u207a-ATP\u9176(Na\u207a\/K\u207a-ATPase)\u662f\u4e00\u7a2e\u5ee3\u6cdb\u5206\u4f48\u65bc\u751f\u7269\u9ad4\u819c\u7cfb\u7d71\u4e2d\u7684\u9176\u3002\u5b83\u5728\u7d30\u80de\u751f\u7406\u4e2d\u767c\u63ee\u591a\u7a2e\u529f\u80fd\uff0c\u53ef\u50ac\u5316 ATP \u6c34\u89e3\u7522\u751f ADP \u548c\u7121\u6a5f\u78f7(inorganic phosphorus) \u8a72\u8a66\u5291\u76d2\u70ba\u6aa2\u6e2c\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u3001\u7d44\u7e54(tissues)\u3001\u7d30\u80de(cells)\u3001\u690d\u7269\u548c\u7d30\u83cc\u7b49\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684 Na+\/K+-ATPase \u6d3b\u6027\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cNa+\/K+-ATP\u9176\u50ac\u5316ATP\u6c34\u89e3\u7522\u751fADP\u548c\u7121\u6a5f\u78f7\u3002\u7121\u6a5f\u78f7\u7684\u542b\u91cf\u53ef\u4ee5\u53cd\u6620ATP\u9176\u7684\u6d3b\u6027\u3002<\/p>\n

\u2022 \u6e2c\u5b9a\u8840\u6e05\u3001\u8840\u6f3f\u3001\u7d44\u7e54\u3001\u7d30\u80de\u3001\u690d\u7269\u548c\u7d30\u83cc\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u672c\u4e2d\u7684Na\u207a\/K\u207a-ATP\u9176\u6d3b\u6027\u3002
\n\u2022 \u63d0\u4f9b\u8a73\u7d30\u7684\u6a23\u54c1\u88fd\u5099\u548c\u7d50\u679c\u8a08\u7b97\u65b9\u6cd5\u3002
\n\u2022 \u64cd\u4f5c\u6b65\u9a5f\u7c21\u55ae\u3002<\/p>\n

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<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Na\u207a\/K\u207a-ATPase Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nThe kit provides a simple method for detecting Na\u207a\/K\u207a-ATPase activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, Na\u207a\/K\u207a-ATPase catalyzes ATP hydrolysis to produce ADP and inorganic phosphorus. The content of inorganic phosphorus can reflect the activity of ATPase.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Extraction Buffer
\n\u2022 Assay Buffer
\n\u2022 Reagent I
\n\u2022 Reagent II
\n\u2022 Reagent III
\n\u2022 Reagent IV
\n\u2022 Reagent V
\n\u2022 Reagent VI
\n\u2022 Reagent VII<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nThe Na\u207a\/K\u207a-ATPase is an enzyme widely distributed in biomembrane system of the organism. It performs several functions in cell physiology and it can catalyze ATP hydrolysis to produce ADP and inorganic phosphorus.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6a23\u54c1\u88fd\u5099(Sample preparation)
\n<\/span><\/strong><\/span>
\n\u5efa\u8b70\u65b0\u9bae\u6a23\u54c1\uff0c\u5982\u4e0d\u7acb\u5373\u6aa2\u6e2c\uff0c\u6a23\u54c1\u53ef\u5728-80\u2103\u4fdd\u5b58\u4e00\u500b\u6708\u3002\u8655\u7406\u5f8c\u7684\u6a23\u54c1\u5fc5\u9808\u7acb\u5373\u9032\u884c\u5206\u6790\u3002
\n1. \u8840\u6e05\u3001\u8840\u6f3f\u6216\u5176\u4ed6\u6db2\u9ad4\u6a23\u672c\uff1a\u76f4\u63a5\u6aa2\u6e2c\u3002
\n2. \u52d5\u7269\u7d44\u7e54\u6a23\u672c\uff1a\u79e4\u53d6 0.1 g \u7d44\u7e54\uff0c\u52a0\u5165 1 mL Extraction Buffer\uff0c\u51b0\u4e0a\u52fb\u6f3f\u3002 4\u2103 8,000g \u96e2\u5fc3 10 \u5206\u9418\u3002\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\u3002\u5c07\u5176\u653e\u5728\u51b0\u4e0a\u9032\u884c\u6e2c\u8a66\u3002
\n3. \u7d30\u80de\u6216\u7d30\u83cc\u6a23\u672c\uff1a\u6bcf\u6b21\u6e2c\u5b9a\u6536\u96c6\u9069\u7576\u6578\u91cf\u7684\u7d30\u80de\u6216\u7d30\u83cc\uff0c\u5728 4\u00b0 12,000 g \u4e0b\u96e2\u5fc3 1 \u5206\u9418\uff0c\u4e26\u4e1f\u68c4\u4e0a\u6e05\u6db2\u3002\u6bcf 5\u00d710 \u6dfb\u52a0 1 mL \u8403\u53d6\u7de9\u885d\u6db2
\n6\u500b\u7d30\u80de\u6216\u7d30\u83cc\u3002\u8d85\u97f3\u6ce2\u7834\u788e\u7d30\u80de\u6216\u7d30\u83cc\uff08\u51b0\u6d74\uff0c\u529f\u738720%\u6216200W\uff0c\u8d85\u97f3\u6ce23\u79d2\uff0c\u9593\u969410\u79d2\uff0c\u91cd\u890730\u6b21\uff09\u3002 4\u2103 8,000 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\u3002\u5c07\u5176\u653e\u5728\u51b0\u4e0a\u9032\u884c\u6e2c\u8a66\u3002
\n4.\u690d\u7269\u7d44\u7e54\u6a23\u672c\uff1a\u7528\u51b7PBS\u6e05\u6d17\u690d\u7269\uff0c\u76e1\u53ef\u80fd\u53bb\u9664\u96dc\u8cea\u3002\u79e4\u53d6 0.1 g \u7d44\u7e54\uff0c\u52a0\u5165 1 mL \u8403\u53d6\u7de9\u885d\u6db2\u4e26\u5728\u51b0\u4e0a\u5747\u8cea\u3002 4\u2103\u30018,000 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\u3002\u4fdd\u7559\u4e00\u6b21\u4ee5\u4f9b\u6e2c\u8a66\u3002\u8a3b\uff1a\u82e5\u4ee5\u86cb\u767d\u6fc3\u5ea6\u8a08\u7b97\u7e3d\u86cb\u767d\u542b\u91cf\uff0c\u6700\u597d\u4f7f\u7528 Protein Quantification Kit (BCA Assay)\uff0c\u8ca8\u865f\uff1aKTD3001 \u5b9a\u91cf\u3002<\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/span><\/p>\n

\u00a0<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Na\u207a\/K\u207a-ATP\u9176\u6d3b\u6027\u5206\u6790\u6aa2\u6e2c | CheKine\u2122 Micro Na\u207a\/K\u207a-ATPase Activi […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[4969],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34449"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=34449"}],"version-history":[{"count":9,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34449\/revisions"}],"predecessor-version":[{"id":50702,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34449\/revisions\/50702"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=34449"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=34449"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=34449"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}