{"id":33789,"date":"2022-08-18T11:32:11","date_gmt":"2022-08-18T03:32:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33789"},"modified":"2024-07-18T19:06:02","modified_gmt":"2024-07-18T11:06:02","slug":"tsh%e7%94%b2%e7%8b%80%e8%85%ba%e4%bf%83%e9%80%b2%e7%b4%a0%e6%aa%a2%e6%b8%ac-elikine-human-tsh-elisa-kit%e8%b2%a8%e8%99%9fket6905","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=33789","title":{"rendered":"TSH\u7532\u72c0\u817a\u4fc3\u9032\u7d20\u6aa2\u6e2c | EliKine\u2122 Human TSH ELISA Kit Abbkine\u8ca8\u865fKTE6905"},"content":{"rendered":"

TSH\u7532\u72c0\u817a\u4fc3\u9032\u7d20\u6aa2\u6e2c | EliKine\u2122 Human TSH ELISA Kit\u8ca8\u865fKTE6905<\/strong>\u00a0<\/a>\u00a00.3 mIU\/L-12 mIU\/L<\/u>, \u6700\u4f4e\u9748\u654f\u5ea60.1 mIU\/L<\/u><\/span><\/span><\/p>\n

<\/p>\n

TSH\u4fc3\u7532\u72c0\u817a\u6fc0\u7d20\uff08Thyroid-stimulating hormone\uff09 \u4e5f\u7a31\u70ba\u4fc3\u7532\u72c0\u817a\u6fc0\u7d20(thyrotropin)\u3001\u4fc3\u7532\u72c0\u817a\u6fc0\u7d20(thyrotropic hormone)\u3001TSH \u6216\u4eba\u985e TSH \u7684 hTSH\uff09\u662f\u4e00\u7a2e\u5782\u9ad4\u6fc0\u7d20(pituitary hormone)\uff0c\u5b83\u523a\u6fc0\u7532\u72c0\u817a(thyroid gland)\u7522\u751f\u7532\u72c0\u817a\u7d20 (thyroxine , T4)\uff0c\u7136\u5f8c\u662f\u4e09\u7898\u7532\u72c0\u817a\u539f\u6c28\u9178 (triiodothyronine, T3)\uff0c\u5b83\u523a\u6fc0\u5e7e\u4e4e\u6240\u6709\u7684\u65b0\u9673\u4ee3\u8b1d\u9ad4\u5167\u7684\u7d44\u7e54\u3002\u5b83\u662f\u7531\u5782\u9ad4\u524d\u8449\u7684\u4fc3\u7532\u72c0\u817a\u7d30\u80de\u5408\u6210\u548c\u5206\u6ccc\u7684\u4e00\u7a2e\u7cd6\u86cb\u767d\u6fc0\u7d20(glycoprotein hormone)\uff0c\u8abf\u7bc0\u7532\u72c0\u817a\u7684\u5167\u5206\u6ccc\u529f\u80fd\u3002 1916 \u5e74\uff0cBennett M. Allen \u548c Philip E. Smith \u767c\u73fe\u5782\u9ad4\u542b\u6709\u4fc3\u7532\u72c0\u817a\u7269\u8cea\u3002TSH\u7532\u72c0\u817a\u4fc3\u9032\u7d20\u6aa2\u6e2c\u8a66\u5291\u76d2, \u63a1\u7528\u5169\u9ede\u593e\u5fc3ELISA(Sandwich ELISA)\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684TSH\u4fc3\u7532\u72c0\u817a\u6fc0\u7d20\uff08Thyroid-stimulating hormone\uff09\u3002\u5075\u6e2c\u7bc4\u570d: 0.3 mIU\/L-12 mIU\/L<\/u><\/strong>, \u6700\u4f4e\u9748\u654f\u5ea60.1 mIU\/L<\/u><\/strong>\u3002<\/u><\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Other biological fluids, Plasma, Serum<\/p>\n

<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nEliKine\u2122 Human TSH ELISA Kit<\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nEliKine\u2122 Human TSH ELISA Kit employs a two-site sandwich ELISA to quantitate TSH in samples. An antibody specific for TSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TSH present is bound by the immobilized antibody. After removing any unbound substances, a HRP conjugated antibody specific for TSH is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSH bound in the initial step. The color development is stopped and the intensity of the color is measured.<\/td>\n<\/tr>\n
Detection method<\/strong><\/td>\nColorimetric<\/td>\n<\/tr>\n
SampleType<\/strong><\/td>\nOther biological fluids, Plasma, Serum<\/td>\n<\/tr>\n
Assay type<\/strong><\/td>\nSandwich ELISA (quantitative)<\/td>\n<\/tr>\n
Assay duration<\/strong><\/td>\nMultiple steps standard sandwich ELISA assay with a working time of 2-3 hours. It depends on the experience of the operation person.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nThyrotropin subunit beta; TSH A; TSH alpha; TSH B; TSH beta; TSHA; TSHB; Thyroid stimulating hormone; Thyroid stimulating hormone alpha chain<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Human TSH microplate
\n\u2022 Human TSH standard
\n\u2022 Human TSH detect antibody
\n\u2022 HRP substrate A
\n\u2022 HRP substrate B
\n\u2022 Stop solution
\n\u2022 Wash buffer
\n\u2022 Plate covers.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nEliKine\u2122 Human TSH ELISA Kit has high sensitivity and excellent specificity for detection of Human TSH. No significant cross-reactivity or interference between Human TSH and analogues was observed.<\/td>\n<\/tr>\n
Calibration range<\/strong><\/td>\n0.3 mIU\/L-12 mIU\/L<\/td>\n<\/tr>\n
Limit of detection<\/strong><\/td>\n0.1 mIU\/L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7269\u7a2e\"\u00a0 \u00a0\"\u7269\u7a2eHUMAN\"\u00a0 \u00a0\"\u7269\u7a2eRAT\"\u00a0\"\u7269\u7a2eporcine\"\u00a0\"\u7269\u7a2erabbit\"<\/p>\n

\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\u6cd5\uff08ELISA\uff09\u662f\u4e00\u7a2e\u5e38\u7528\u65bc\u6e2c\u91cf\u751f\u7269\u6a23\u672c\u4e2d\u7684\u6297\u9ad4(antibodies)\u3001\u6297\u539f( antigens)\u3001\u86cb\u767d\u8cea(proteins)\u548c\u7cd6\u86cb\u767d( glycoproteins )\u7684\u514d\u75ab\u5b78\u6e2c\u5b9a\u3002\u4e00\u4e9b\u4f8b\u5b50\u5305\u62ec\uff1aHIV \u611f\u67d3( HIV infection)\u7684\u8a3a\u65b7\u3001\u598a\u5a20\u8a66\u9a57(pregnancy tests)\u4ee5\u53ca\u7d30\u80de\u4e0a\u6e05\u6db2\u6216\u8840\u6e05\u4e2d\u7d30\u80de\u56e0\u5b50(cytokines)\u6216\u53ef\u6eb6\u6027\u53d7\u9ad4(soluble receptors)\u7684\u6e2c\u91cf\u3002 ELISA\u539f\u7406\u53ef\u5206\u70ba\u593e\u5fc3ELISA(sandwich ELISA)\u3001\u7af6\u722d\u6027ELISA(competitive ELISA )\u548c\u9593\u63a5ELISA(competitive ELISA )\u3002<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/strong><\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u529f\u80fd<\/strong><\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u5177\u6709\u591a\u7a2e\u751f\u7269\u5b78\u529f\u80fd\u3002\u5b83\u662f\u6025\u6027\u671f\u53cd\u61c9(acute phase response)\u7684\u6709\u6548\u8a98\u5c0e\u5291\u3002\u5728B\u7d30\u80de(B-cells)\u6700\u7d42\u5206\u5316\u70baIg\u5206\u6ccc\u7d30\u80de(Ig-secreting cells)\u7684\u6700\u7d42\u5206\u5316\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u53c3\u8207\u6dcb\u5df4\u7d30\u80de(lymphocyte)\u548c\u55ae\u6838\u7d30\u80de(monocyte)\u7684\u5206\u5316\u3002\u5b83\u8a98\u5c0e\u9aa8\u9ad3\u7624(myeloma)\u548c\u6f3f\u7d30\u80de\u7624(plasmacytoma growth)\u751f\u9577\uff0c\u4e26\u8a98\u5c0e\u795e\u7d93\u7d30\u80de\u5206\u5316(differentiation)\u3002\u4f5c\u7528\u65bcB\u7d30\u80de(B-cells)\uff0cT\u7d30\u80de(T-cells)\uff0c\u809d\u7d30\u80de(hepatocytes)\uff0c\u9020\u8840\u7956\u7d30\u80de(hematopoeitic progenitor cells)\u548cCNS\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71\u7d30\u80de\u3002\u4e5f\u53ef\u4ee5\u4f5c\u70ba\u808c\u8089\u56e0\u5b50(myokine)\u3002\u5b83\u5728\u808c\u8089\u6536\u7e2e\u5f8c\u6392\u5165\u8840\u6db2\uff0c\u4e26\u8d77\u5230\u589e\u52a0\u8102\u80aa\u5206\u89e3\u548c\u6539\u5584\u80f0\u5cf6\u7d20\u62b5\u6297(insulin resistance)\u7684\u4f5c\u7528\u3002<\/p>\n

\u708e\u6027\u7d30\u80de\u56e0\u5b50<\/strong><\/span><\/p>\n

\u708e\u75c7\u7531\u591a\u7a2e\u53ef\u6eb6\u6027\u56e0\u5b50\u4ecb\u5c0e\uff0c\u5305\u62ec\u4e00\u7d44\u7a31\u70ba\u7d30\u80de\u56e0\u5b50\u7684\u5206\u6ccc\u591a\u80bd\u3002\u708e\u6027\u7d30\u80de\u56e0\u5b50\u53ef\u5206\u70ba\u5169\u985e\uff1a\u53c3\u8207\u6025\u6027\u708e\u75c7\u7684\u7d30\u80de\u56e0\u5b50\u548c\u8ca0\u8cac\u4efb\u7684\u7d30\u80de\u56e0\u5b50\u3002\u708e\u75c7\u662f\u7d44\u7e54\u5c0d\u640d\u50b7\u7684\u53cd\u61c9\uff0c\u5176\u7279\u5fb5\u662f\u5728\u6025\u6027\u671f\u4ee5\u589e\u52a0\u7684\u8840\u6d41\u91cf\u548c\u8840\u7ba1\u901a\u900f\u6027\u4ee5\u53ca\u9ad4\u6db2\uff0c\u767d\u7d30\u80de\u548c\u708e\u6027\u4ecb\u8cea\uff08\u4f8b\u5982\u7d30\u80de\u56e0\u5b50\uff09\u7684\u7a4d\u7d2f\u70ba\u7279\u5fb5\u3002\u5e7e\u7a2e\u7d30\u80de\u56e0\u5b50\u5728\u4ecb\u5c0e\u6025\u6027\u708e\u75c7\u53cd\u61c9\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\uff0c\u5373IL-1\uff0cTNF-\u03b1\uff0cIL-6\uff0cIL-11\uff0cIL-8\u548c\u5176\u4ed6\u8da8\u5316\u56e0\u5b50\uff0cG-CSF\u548cGM-CSF\u3002<\/p>\n

\u5df2\u77e5\u53ef\u4ecb\u5c0e\u6162\u6027\u708e\u75c7\u904e\u7a0b\u7684\u7d30\u80de\u56e0\u5b50\u53ef\u5206\u70ba\u53c3\u8207\u9ad4\u6db2\u708e\u75c7\u7684\u7d30\u80de\u56e0\u5b50\uff0c\u4f8b\u5982IL-3\uff0cIL-4\uff0cIL-5\uff0cIL-6\uff0cIL-7\uff0cIL-9\uff0cIL-10\uff0cIL- 13\uff0c\u8f49\u5316\u751f\u9577\u56e0\u5b50b\uff08TGF-b\uff09\uff0c\u4ee5\u53ca\u90a3\u4e9b\u6709\u52a9\u65bc\u7d30\u80de\u708e\u75c7\u7684\u56e0\u5b50\uff0c\u4f8b\u5982IL-1\uff0cIL-2\uff0cIL-3\uff0cIL-4\uff0cIL-7\uff0cIL-9\uff0cIL-10\uff0c IL-12\uff0c\u5e72\u64fe\u7d20\uff08IFN\uff09\uff0cIFN-g\u8a98\u5c0e\u56e0\u5b50\uff08IGIF\uff09\uff0cTGF-b\u548cTNF-a\u548c-b\u3002\u708e\u75c7\u7570\u5e38\u662f\u4e00\u5927\u985e\u75be\u75c5\uff0c\u662f\u8a31\u591a\u4eba\u985e\u75be\u75c5\u7684\u57fa\u790e\u3002\u7d30\u80de\u56e0\u5b50\u662f\u5bbf\u4e3b\u5c0d\u611f\u67d3\uff0c\u514d\u75ab\u53cd\u61c9\uff0c\u708e\u75c7\u548c\u5275\u50b7\u53cd\u61c9\u7684\u8abf\u7bc0\u5291\u3002\u4e00\u4e9b\u7d30\u80de\u56e0\u5b50\u8d77\u4f7f\u75be\u75c5\u60e1\u5316\u7684\u4f5c\u7528\uff08\u4fc3\u708e\u7d30\u80de\u56e0\u5b50\uff09\uff0c\u800c\u53e6\u4e00\u4e9b\u7d30\u80de\u56e0\u5b50\u8d77\u6e1b\u8f15\u708e\u75c7\u548c\u4fc3\u9032\u7652\u5408\u7684\u4f5c\u7528\uff08\u6297\u708e\u7d30\u80de\u56e0\u5b50\uff09\u3002<\/p>\n

\u767d\u4ecb\u7d20-1\uff08Interleukin-1, IL-1\uff09\u662f\u6709\u53f2\u4ee5\u4f86\u6700\u65e9\u7684\u7d30\u80de\u56e0\u5b50(cytokines)\u4e4b\u4e00\u3002\u5b83\u7684\u6700\u521d\u767c\u73fe\u662f\u53ef\u80fd\u8a98\u767c\u767c\u71d2(fever)\uff0c\u63a7\u5236\u6dcb\u5df4\u7d30\u80de(lymphocytes)\uff0c\u589e\u52a0\u9aa8\u9ad3\u7d30\u80de\u6578\u91cf(bone marrow cells)\u4e26\u5f15\u8d77\u9aa8\u95dc\u7bc0\u9000\u5316\u7684\u56e0\u7d20\u3002\u6b64\u6642\uff0cIL-1\u4ee5\u5176\u4ed6\u5e7e\u7a2e\u540d\u7a31\u70ba\u4eba\u6240\u77e5\uff0c\u5305\u62ec\u5167\u6e90\u6027\u71b1\u539f(endogenous pyrogen)\uff0c\u6dcb\u5df4\u7d30\u80de\u6d3b\u5316\u56e0\u5b50(lymphocyte activating factor)\uff0c\u9020\u8840\u7d20-1(haemopoetin-1)\u548c\u55ae\u6838\u7d30\u80de\u56e0\u5b50(mononuclear cell factor)\u7b49\u3002\u5927\u7d04\u57281984\u5e74\u81f31985\u5e74\u9593\uff0c\u79d1\u5b78\u5bb6\u5011\u8b49\u5be6IL-1\u5be6\u969b\u4e0a\u7531\u5169\u7a2e\u4e0d\u540c\u7684\u86cb\u767d\u8cea\u7d44\u6210\uff0c\u73fe\u5728\u7a31\u70baIL-1\u03b1\u548cIL-1\u03b2\uff0c\u5b83\u5011\u5c6c\u65bc\u7a31\u70ba\u767d\u7d30\u80de\u4ecb\u7d201\u8d85\u5bb6\u65cf\u7684\u7d30\u80de\u56e0\u5b50\u5bb6\u65cf\u3002 IL-1\u03b2\u7531\u5de8\u566c\u7d30\u80de(macrophages)\uff0c\u55ae\u6838\u7d30\u80de(monocytes)\u548c\u6a39\u7a81\u72c0\u7d30\u80de(dendritic cells)\u7522\u751f\u3002<\/p>\n

IFN-\u03b3\uff08IFN-gamma, \u5e72\u64fe\u7d20-\u03b3\uff09\u662f\u4fc3\u708e(proinflammatory)\u7d30\u80de\u56e0\u5b50(cytokine)\u7684\u539f\u578b(prototype)\uff0c\u7531\u591a\u7a2e\u514d\u75ab\u7d30\u80de(immune cells)\u5728\u708e\u6027(inflammatory)\u689d\u4ef6\u4e0b\u7522\u751f\uff0c\u7279\u5225\u662f\u7531T\u7d30\u80de(T cells)\u548cNK\u7d30\u80de(NK cells)\u7522\u751f\u3002\u5b83\u901a\u904e\u4fc3\u9032Th1\u7d30\u80de\u7684\u767c\u80b2(development)\u548c\u6fc0\u6d3b(activation)\uff0c\u55ae\u6838\u7d30\u80de(monocytes)\u548c\u5de8\u566c\u7d30\u80de(macrophage)\u7684\u5316\u5b78\u5f15\u8a98(chemoattraction)\u548c\u6fc0\u6d3b(activation)\uff0c\u6297\u539f\u5448\u905e\u5206\u5b50(antigen presentation molecules)\u7684\u4e0a\u8abf\u4ee5\u53caB\u7d30\u80de\u4e2d\u7684\u514d\u75ab\u7403\u86cb\u767d\u985e\u5225\u8f49\u63db(immunoglobulin class switching)\uff0c\u5728\u5bbf\u4e3b\u9632\u79a6\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\u3002\u5b83\u9084\u5177\u6709\u6297\u75c5\u6bd2(antiviral)\uff0c\u6297\u589e\u6b96(antiproliferative)\u548c\u51cb\u4ea1\u4f5c\u7528(apoptotic)\u3002\u53e6\u5916\uff0cIFN-\u03b3\u901a\u904e\u4fc3\u9032\u8abf\u7bc0\u6027T\u7d30\u80de\u7684\u767c\u80b2\u4e26\u6291\u5236Th17\u7d30\u80de\u5206\u5316\u800c\u5145\u7576\u6297\u708e\u4ecb\u8cea\u3002 IFN-\u03b3\u4e8c\u805a\u9ad4\u901a\u904e\u5169\u500bIFN-\u03b3R1\u548c\u5169\u500bIFN-\u03b3R2\u4e9e\u57fa\u7684\u53d7\u9ad4\u8907\u5408\u7269\u767c\u51fa\u4fe1\u865f\u3002<\/p>\n

\u767d\u7d30\u80de\u4ecb\u7d2017 (Interleukin 17, IL17)<\/p>\n

IL-17\u57fa\u56e0\u7de8\u78bc( IL-17 gene)\u7684\u86cb\u767d\u8cea\u662f\u7531\u6d3b\u5316\u7684T\u7d30\u80de(activated T cells)\u6240\u7522\u751f\u7684\u4fc3\u767c\u708e\u7d30\u80de\u6fc0\u7d20\u3002\u6b64\u7d30\u80de\u6fc0\u7d20\u8abf\u7bc0 NF-\u03baB \u548c\u7d72\u88c2\u539f\u6d3b\u5316\u86cb\u767d\u6fc0\u9176(mitogen-activated protein kinases)\u7684\u6d3b\u6027\u3002\u9019\u7a2e\u7d30\u80de\u6fc0\u7d20(cytokine )\u53ef\u4ee5\u523a\u6fc0 IL6 \u548c\u74b0\u6c27\u5408\u9176-2 (cyclooxygenase-2, PTGS2\/COX-2) \u7684\u8868\u9054\uff0c\u4e26\u589e\u5f37\u4e00\u6c27\u5316\u6c2e (nitric oxide, NO) \u7684\u7522\u751f\u3002\u9ad8\u6c34\u5e73\u7684\u9019\u7a2e\u7d30\u80de\u6fc0\u7d20\u8207\u591a\u7a2e\u6162\u6027\u767c\u708e\u6027\u75be\u75c5\u6709\u95dc\uff0c\u5305\u62ec\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\u3001\u725b\u76ae\u766c(psoriasis )\u548c\u591a\u767c\u6027\u786c\u5316\u75c7(multiple sclerosis)\u3002<\/p>\n

\u767d\u8840\u7403\u4ecb\u7d20-8\uff08Interleukin-8, IL8)<\/p>\n

IL-8\u57fa\u56e0\u7de8\u78bc(IL-8 gene)\u7684\u86cb\u767d\u8cea\u662fCXC\u8da8\u5316\u56e0\u5b50\u5bb6\u65cf(CXC chemokine family)\u7684\u6210\u54e1\u3002\u9019\u7a2e\u8da8\u5316\u56e0\u5b50(chemokine )\u662f\u767c\u708e\u53cd\u61c9(inflammatory response)\u7684\u4e3b\u8981\u4ecb\u8cea\u4e4b\u4e00\u3002\u9019\u7a2e\u8da8\u5316\u56e0\u5b50\u7531\u591a\u7a2e\u7d30\u80de\u985e\u578b\u5206\u6ccc\u3002\u5b83\u5177\u6709\u5316\u5b78\u5f15\u8a98\u5291( chemoattractant)\u7684\u4f5c\u7528\uff0c\u4e5f\u662f\u4e00\u7a2e\u6709\u6548\u7684\u8840\u7ba1\u751f\u6210\u56e0\u5b50(angiogenic factor)\u3002 IL-8\u57fa\u56e0\u88ab\u8a8d\u70ba\u5728\u7d30\u652f\u6c23\u7ba1\u708e\uff08\u4e00\u7a2e\u7531\u75c5\u6bd2\u611f\u67d3\u5f15\u8d77\u7684\u5e38\u898b\u547c\u5438\u9053\u75be\u75c5\uff09\u7684\u767c\u75c5\u6a5f\u5236(pathogenesis )\u4e2d\u767c\u63ee\u4f5c\u7528\u3002 IL-8\u57fa\u56e0(IL-8 gene )\u548cCXC\u8da8\u5316\u56e0\u5b50\u57fa\u56e0\u5bb6\u65cf(CXC chemokine gene family)\u7684\u5176\u4ed610\u500b\u6210\u54e1\u5728\u5b9a\u4f4d\u65bc\u67d3\u8272\u9ad44q( chromosome 4q)\u7684\u5340\u57df\u4e2d\u5f62\u6210\u8da8\u5316\u56e0\u5b50\u57fa\u56e0\u7c07(chemokine gene cluster )\u3002<\/p>\n

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