{"id":33758,"date":"2022-08-09T16:08:17","date_gmt":"2022-08-09T08:08:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33758"},"modified":"2022-08-30T14:01:44","modified_gmt":"2022-08-30T06:01:44","slug":"ddddk-tag%e5%96%ae%e6%a0%aa%e6%8a%97%e9%ab%941b10-abbkine%e8%b2%a8%e8%99%9fabt2010","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=33758","title":{"rendered":"DDDDK Tag\u55ae\u682a\u6297\u9ad4(1B10) | Abbkine\u8ca8\u865fABT2010"},"content":{"rendered":"

DDDDK Tag\u55ae\u682a\u6297\u9ad4(1B10) | <\/strong>Abbkine\u8ca8\u865f<\/strong>ABT2010<\/a><\/span><\/p>\n

Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"DYKDDDDK \u80bd\uff08Flag-tag\uff09\u662f\u4e00\u7a2e\u591a\u80bd\u86cb\u767d\u8cea\u6a19\u7c64(polypeptide protein tag)\uff0c\u53ef\u4ee5\u4f7f\u7528\u91cd\u7d44 DNA \u6280\u8853\u6dfb\u52a0\u5230\u86cb\u767d\u8cea\u4e2d\u3002\u5b83\u53ef\u7528\u65bc\u89aa\u548c\u5c64\u6790\uff0c\u7136\u5f8c\u7528\u65bc\u5c07\u91cd\u7d44\u7684\u904e\u8868\u9054\u86cb\u767d\u8207\u5bbf\u4e3b\u751f\u7269\u8868\u9054\u7684\u91ce\u751f\u578b\u86cb\u767d\u5206\u96e2\u3002\u5b83\u9084\u53ef\u7528\u65bc\u5206\u96e2\u5177\u6709\u591a\u500b\u4e9e\u57fa\u7684\u86cb\u767d\u8cea\u8907\u5408\u7269\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u00a0DDDDK Tag\u55ae\u682a\u6297\u9ad4(1B10)\uff0c\u514d\u75ab\u539fSynthetic Peptide\u3002\u901a\u904e\u4f7f\u7528\u7279\u7570\u6027\u514d\u75ab\u539f\u7684\u89aa\u548c\u5c64\u6790\u5f9e\u5154\u6297\u8840\u6e05\u4e2d\u89aa\u548c\u7d14\u5316\u6297\u9ad4\u3002\u53ef\u61c9\u7528\u65bcIF, IP, WB\u5be6\u9a57\u3002\u6700\u4f73\u5de5\u4f5c\u7a00\u91cb\u5ea6\u61c9\u7531\u7814\u7a76\u8005\u901a\u904e\u5be6\u9a57\u78ba\u5b9a\u3002\u5efa\u8b70\u7684\u8d77\u59cb\u7a00\u91cb\u5ea6\u5982\u4e0b\uff1aSuggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).\u3002\u6fc3\u5ea61 mg\/ml\uff0c\u4fdd\u5b58\u65bcLiquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

\"A02010-if\"<\/p>\n

Fig.1. Immunofluorescence staining (1:4000) of Flag fusion protein in 293 cells and counterstained with DAPI.<\/p>\n

\"A02010-wb\"<\/p>\n

Fig.2. IP (1:400)-WB (1:10000) analysis of Flag fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with Flag-tag protein (lane B), IP transfected 293 with normal Mouse IgG and Protein G agarose (lane C), IP transfected 293 with Anti Flag tag mAb and Protein G agarose (lane D), and IP transfected 293 with only Protein G agarose (lane E).<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nSynthetic Peptide<\/td>\n<\/tr>\n
Host<\/strong><\/td>\nMouse<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIF, IP, WB<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nOptimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:4000), IP (1:400).<\/td>\n<\/tr>\n
Clonality<\/strong><\/td>\nMonoclonal<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nDDDDK tag; DYKDDDDK tag; Flag tag; DYKDDDDK epitope; DDK epitope<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1 mg\/ml<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nLiquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at -20\u00b0C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u9810\u67d3protein marker<\/a> | \u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291\u00a0<\/a> | \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/p>\n

Abbkine\u63d0\u4f9b\u4e00\u7cfb\u5217\u9ad8\u5ea6\u7279\u7570\u6027\u55ae\u514b\u9686\u548c\u591a\u514b\u9686\u6297\u9ad4\uff0ctag antibody\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\u5982 His tag\u3001GST tag\u3001S tag\u3001HA tag\u3001DDDDK tag\u3001Myc tag\u3001MBP tag\u548c mCherry tag\u7b49\u55ae\/\u591a\u682aTag\u6297\u9ad4\u3002\u53e6\u5177\u751f\u7269\u7d20(Biotin)\u3001HRP\u3001AbFluor\u2122 \u67d3\u6599\u3001\u74ca\u8102\u7cd6(agarose)\u548c\u78c1\u73e0\u5076\u806f\u7269(magnetic beads)\u6a19\u8a8c\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat.No<\/td>\nName<\/td>\nSize1<\/td>\n<\/tr>\n
KTD104-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Magnetic Beads)<\/td>\n20T<\/td>\n<\/tr>\n
KTD105-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Agarose)<\/td>\n20T<\/td>\n<\/tr>\n
A25012<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25022<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25112<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25122<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25222<\/td>\nIPKine\u2122 HRP, Goat Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
KTI2014<\/td>\nIPKine\u2122 Anti-DDDDK Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2024<\/td>\nIPKine\u2122 Anti-GFP Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2034<\/td>\nIPKine\u2122 Anti-GST Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2044<\/td>\nIPKine\u2122 Anti-HA Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2064<\/td>\nIPKine\u2122 Anti-Myc Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
ABT2013<\/td>\nAgarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2014<\/td>\nMagnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2024<\/td>\nMagnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2023<\/td>\nAgarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2043<\/td>\nAgarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2044<\/td>\nMagnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2053<\/td>\nAgarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2054<\/td>\nMagnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2063<\/td>\nAgarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2064<\/td>\nMagnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2173<\/td>\nAgarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2174<\/td>\nMagnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2010<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2011<\/td>\nAnti-DDDDK Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2015<\/td>\nHRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2020<\/td>\nAnti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2025<\/td>\nHRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2021<\/td>\nAnti-GFP Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2030<\/td>\nAnti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2035<\/td>\nHRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2040<\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2041<\/td>\nAnti-HA Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2045<\/td>\nHRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2050<\/td>\nAnti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2051<\/td>\nAnti-His Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2055<\/td>\nHRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2060<\/td>\nAnti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2061<\/td>\nAnti-Myc Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2065<\/td>\nHRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2170<\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2070<\/td>\nAnti-MBP Tag Mouse Monoclonal Antibody (9Y5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2080<\/td>\nAnti-mCherry Tag Mouse Monoclonal Antibody (9D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2090<\/td>\nAnti-E2 Tag Mouse Monoclonal Antibody (12T4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2100<\/td>\nAnti-HSV Tag Mouse Monoclonal Antibody (16T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2110<\/td>\nAnti-KT3 Tag Mouse Monoclonal Antibody (14D8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2120<\/td>\nAnti-RFP Tag Mouse Monoclonal Antibody (9D1)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2130<\/td>\nAnti-S Tag Mouse Monoclonal Antibody (9T10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2150<\/td>\nAnti-T7 Tag Mouse Monoclonal Antibody (6D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2160<\/td>\nAnti-Trx Tag Mouse Monoclonal Antibody (14D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2180<\/td>\nAnti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2190<\/td>\nAnti-CBP Tag Monoclonal Antibody (12H5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2200<\/td>\nAnti-TAP Tag Monoclonal Antibody (4H2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2210<\/td>\nAnti-Avi-Tag Monoclonal Antibody (5G11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2220<\/td>\nAnti-SRT-Tag Monoclonal Antibody (11G3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2230<\/td>\nAnti-Strep-Tag II Monoclonal Antibody (8C12)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2240<\/td>\nAnti-mStrawberry Monoclonal Antibody (4C9)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2250<\/td>\nAnti-EYFP-Tag Monoclonal Antibody (10T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2260<\/td>\nAnti-mOrange Monoclonal Antibody (9A10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2270<\/td>\nAnti-AmCyan Monoclonal Antibody (8T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2280<\/td>\nAnti-ECFP-Tag Monoclonal Antibody (6B11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2290<\/td>\nAnti-EBFP Monoclonal Antibody (8B5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2300<\/td>\nAnti-Nano-Tag9 Monoclonal Antibody (11T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

DDDDK Tag\u55ae\u682a\u6297\u9ad4(1B10) | Abbkine\u8ca8\u865fABT2010 Anti-DDDDK Tag M […]<\/p>\n","protected":false},"author":3,"featured_media":33760,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[476],"tags":[4894],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33758"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33758"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33758\/revisions"}],"predecessor-version":[{"id":33761,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33758\/revisions\/33761"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33760"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33758"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33758"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33758"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}