{"id":33732,"date":"2022-08-09T11:38:43","date_gmt":"2022-08-09T03:38:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33732"},"modified":"2025-09-23T16:18:22","modified_gmt":"2025-09-23T08:18:22","slug":"ha-tag%e5%96%ae%e6%a0%aa%e6%8a%97%e9%ab%94-4f6-abbkine%e8%b2%a8%e8%99%9fabt2040","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=33732","title":{"rendered":"HA Tag\u55ae\u682a\u6297\u9ad4 (4F6) | Abbkine\u8ca8\u865fABT2040"},"content":{"rendered":"

HA Tag\u55ae\u682a\u6297\u9ad4 (4F6) | <\/strong>Abbkine\u8ca8\u865f<\/strong>ABT2040<\/a><\/span><\/p>\n

Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/p>\n

HA Tag\u55ae\u682a\u6297\u9ad4\uff0c\u514d\u75ab\u539fSynthetic Peptide\u3002\u901a\u904e\u4f7f\u7528\u7279\u7570\u6027\u514d\u75ab\u539f\u7684\u89aa\u548c\u5c64\u6790\u5f9e\u5154\u6297\u8840\u6e05\u4e2d\u89aa\u548c\u7d14\u5316\u6297\u9ad4\u3002\u53ef\u61c9\u7528\u65bcIF, IP, WB\u5be6\u9a57\u3002\u6700\u4f73\u5de5\u4f5c\u7a00\u91cb\u5ea6\u61c9\u7531\u7814\u7a76\u8005\u901a\u904e\u5be6\u9a57\u78ba\u5b9a\u3002\u5efa\u8b70\u7684\u8d77\u59cb\u7a00\u91cb\u5ea6\u5982\u4e0b\uff1aSuggested starting dilutions are as follows: WB (1:10000), IF (1:2000), IP (1:400)\u3002\u6fc3\u5ea61mg\/ml\uff0c\u4fdd\u5b58\u65bcLiquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

\"A02040-if\"<\/p>\n

Fig.1. Immunofluorescence staining (1:2000) of HA fusion protein in 293 cells with red and counterstained with DAPI.<\/p>\n

\"A02040-wb\"<\/p>\n

Fig.2.IP (1:400)-WB (1:10000) analysis of HA fusion protein expression in 293 cells. Untransfected 293 cell lysate (lane A), transfected 293 cell lysate with HA-tag protein (lane B); IP untransfected 293 cell lysate with Anti HA tag mAb (lane C); IP transfected 293 cell lysate with normal Mouse IgG (lane D), or with Anti HA tag mAb (lane E), and IP transfected 293 without both normal Mouse IgG and HA tag mAb (lane F).<\/p>\n

\"abbkine02\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nSynthetic Peptide<\/td>\n<\/tr>\n
Host<\/strong><\/td>\nMouse<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIF, IP, WB<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nOptimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:10000), IF (1:2000), IP (1:400).<\/td>\n<\/tr>\n
Clonality<\/strong><\/td>\nMonoclonal<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nHA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1 mg\/ml<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nLiquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at -20\u00b0C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nHA epitope; HA1; HA2; hemagglutinin; Hemagglutinin HA1 chain; Hemagglutinin HA2 chain<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u9810\u67d3protein marker<\/a> | \u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291\u00a0<\/a> | \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/p>\n

Abbkine\u63d0\u4f9b\u4e00\u7cfb\u5217\u9ad8\u5ea6\u7279\u7570\u6027\u55ae\u514b\u9686\u548c\u591a\u514b\u9686\u6297\u9ad4\uff0ctag antibody\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\u5982 His tag\u3001GST tag\u3001S tag\u3001HA tag\u3001DDDDK tag\u3001Myc tag\u3001MBP tag\u548c mCherry tag\u7b49\u55ae\/\u591a\u682aTag\u6297\u9ad4\u3002\u53e6\u5177\u751f\u7269\u7d20(Biotin)\u3001HRP\u3001AbFluor\u2122 \u67d3\u6599\u3001\u74ca\u8102\u7cd6(agarose)\u548c\u78c1\u73e0\u5076\u806f\u7269(magnetic beads)\u6a19\u8a8c\u3002<\/p>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat.No<\/td>\nName<\/td>\nSize1<\/td>\n<\/tr>\n
KTD104-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Magnetic Beads)<\/td>\n20T<\/td>\n<\/tr>\n
KTD105-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Agarose)<\/td>\n20T<\/td>\n<\/tr>\n
A25012<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25022<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25112<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25122<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25222<\/td>\nIPKine\u2122 HRP, Goat Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
KTI2014<\/td>\nIPKine\u2122 Anti-DDDDK Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2024<\/td>\nIPKine\u2122 Anti-GFP Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2034<\/td>\nIPKine\u2122 Anti-GST Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2044<\/td>\nIPKine\u2122 Anti-HA Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2064<\/td>\nIPKine\u2122 Anti-Myc Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
ABT2013<\/td>\nAgarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2014<\/td>\nMagnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2024<\/td>\nMagnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2023<\/td>\nAgarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2043<\/td>\nAgarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2044<\/td>\nMagnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2053<\/td>\nAgarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2054<\/td>\nMagnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2063<\/td>\nAgarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2064<\/td>\nMagnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2173<\/td>\nAgarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2174<\/td>\nMagnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2010<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2011<\/td>\nAnti-DDDDK Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2015<\/td>\nHRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2020<\/td>\nAnti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2025<\/td>\nHRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2021<\/td>\nAnti-GFP Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2030<\/td>\nAnti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2035<\/td>\nHRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2040<\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2041<\/td>\nAnti-HA Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2045<\/td>\nHRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2050<\/td>\nAnti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2051<\/td>\nAnti-His Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2055<\/td>\nHRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2060<\/td>\nAnti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2061<\/td>\nAnti-Myc Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2065<\/td>\nHRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2170<\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2070<\/td>\nAnti-MBP Tag Mouse Monoclonal Antibody (9Y5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2080<\/td>\nAnti-mCherry Tag Mouse Monoclonal Antibody (9D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2090<\/td>\nAnti-E2 Tag Mouse Monoclonal Antibody (12T4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2100<\/td>\nAnti-HSV Tag Mouse Monoclonal Antibody (16T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2110<\/td>\nAnti-KT3 Tag Mouse Monoclonal Antibody (14D8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2120<\/td>\nAnti-RFP Tag Mouse Monoclonal Antibody (9D1)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2130<\/td>\nAnti-S Tag Mouse Monoclonal Antibody (9T10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2150<\/td>\nAnti-T7 Tag Mouse Monoclonal Antibody (6D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2160<\/td>\nAnti-Trx Tag Mouse Monoclonal Antibody (14D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2180<\/td>\nAnti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2190<\/td>\nAnti-CBP Tag Monoclonal Antibody (12H5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2200<\/td>\nAnti-TAP Tag Monoclonal Antibody (4H2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2210<\/td>\nAnti-Avi-Tag Monoclonal Antibody (5G11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2220<\/td>\nAnti-SRT-Tag Monoclonal Antibody (11G3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2230<\/td>\nAnti-Strep-Tag II Monoclonal Antibody (8C12)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2240<\/td>\nAnti-mStrawberry Monoclonal Antibody (4C9)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2250<\/td>\nAnti-EYFP-Tag Monoclonal Antibody (10T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2260<\/td>\nAnti-mOrange Monoclonal Antibody (9A10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2270<\/td>\nAnti-AmCyan Monoclonal Antibody (8T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2280<\/td>\nAnti-ECFP-Tag Monoclonal Antibody (6B11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2290<\/td>\nAnti-EBFP Monoclonal Antibody (8B5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2300<\/td>\nAnti-Nano-Tag9 Monoclonal Antibody (11T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

HA Tag\u55ae\u682a\u6297\u9ad4 (4F6) | Abbkine\u8ca8\u865fABT2040 Anti-HA Tag Mouse M […]<\/p>\n","protected":false},"author":3,"featured_media":33734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[476],"tags":[4889],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33732"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33732"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33732\/revisions"}],"predecessor-version":[{"id":52978,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33732\/revisions\/52978"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33734"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33732"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33732"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33732"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}