{"id":33536,"date":"2022-07-29T15:32:38","date_gmt":"2022-07-29T07:32:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33536"},"modified":"2024-01-12T17:49:32","modified_gmt":"2024-01-12T09:49:32","slug":"%e8%86%9c%e7%b4%b0%e8%83%9e%e8%b3%aa%e8%9b%8b%e7%99%bd%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92-exkine-membrane-and-cytoplasmic-protein-extraction-kit%e8%b2%a8%e8%99%9fktp3005","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=33536","title":{"rendered":"\u819c&\u7d30\u80de\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2 | ExKine\u2122 Membrane and Cytoplasmic Protein Extraction kit\u8ca8\u865fKTP3005"},"content":{"rendered":"

\u819c&\u7d30\u80de\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2 | ExKine\u2122 Membrane and Cytoplasmic Protein Extraction kit\u8ca8\u865fKTP3005<\/a><\/span><\/p>\n

\u819c(membrane)\u63d0\u53d6\u7269\u901a\u5e38\u662f\u7814\u7a76\u819c\u86cb\u767d(membrane proteins)\u53ca\u5176\u76f8\u4e92\u4f5c\u7528(interactions)\u7684\u7b2c\u4e00\u6b65\u3002\u6240\u5f97\u88fd\u5291\u53ef\u76f4\u63a5\u7528\u65bc\u86cb\u767d\u8cea\u5370\u8de1(Western blotting)\u3001\u6591\u9ede\u5370\u8de1(Dot blot)\u3001\u514d\u75ab\u6c89\u6fb1(Immunoprecipitation)\uff0c\u6216\u4f5c\u70ba\u7d14\u5316\u7279\u5b9a\u86cb\u767d\u8cea\u7684\u8d77\u9ede\u3002\u7d30\u80de\u8cea(Cytoplasm)\u63d0\u53d6\u7269\u7684\u88fd\u5099\u901a\u5e38\u662f\u7814\u7a76\u7d30\u80de\u8cea\u86cb\u767d\u53ca\u5176\u76f8\u4e92\u4f5c\u7528\u7684\u7b2c\u4e00\u6b65\u3002\u6240\u5f97\u88fd\u5291\u53ef\u76f4\u63a5\u7528\u65bc\u86cb\u767d\u8cea\u5370\u8de1(Western blotting)\u3001\u96fb\u6cf3\u9077\u79fb\u7387\u8b8a\u52d5\u5206\u6790 (Electrophoresis Mobility Shift Assay, EMSA)\u3001\u8db3\u8de1\u5206\u6790(footprinting analysis)\u3001\u8f49\u9304\u5206\u6790(transcription assays)\uff0c\u6216\u4f5c\u70ba\u7d14\u5316\u8abf\u7bc0\u86cb\u767d(regulatory proteins)\u7684\u8d77\u9ede\u3002The Abbkine ExKine\u2122 \u819c(Membrane)\u548c\u7d30\u80de\u8cea(Cytoplasmic Protein)\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2\u80fd\u5920\u5f9e\u54fa\u4e73\u52d5\u7269\u57f9\u990a\u7684\u7d30\u80de\u6216\u7d44\u7e54\u4e2d\u9010\u6b65\u5206\u96e2\u548c\u88fd\u5099\u975e\u8b8a\u6027(non-denatured)\u3001\u6d3b\u6027\u7684\u7d30\u80de\u8cea(active cytoplasmic)\u548c\u819c\u86cb\u767d(membrane protein)\uff0c\u53ef\u76f4\u63a5\u7528\u65bc\u5404\u7a2e\u86cb\u767d\u8cea\u9ad4\u5b78(proteomics application)\u61c9\u7528\u3002\u7d30\u80de\u9996\u5148\u901a\u904e\u52fb\u6f3f(homogenization)\u7834\u788e\u4ee5\u91cb\u653e\u7d30\u80de\u6eb6\u8cea(cytosolic)\u548c\u819c\u86cb\u767d(membrane proteins)\uff0c\u7136\u5f8c\u901a\u904e\u9ad8\u901f\u96e2\u5fc3\u7372\u5f97\u819c\u86cb\u767d\u3002\u63d0\u53d6\u6548\u7387\u548c\u7522\u91cf\u5c07\u6839\u64da\u7d30\u80de\u985e\u578b\u4ee5\u53ca\u6574\u5408\u819c\u86cb\u767d\u8de8\u8d8a\u8102\u8cea\u96d9\u5c64(lipid bilayer)\u7684\u6b21\u6578\u800c\u6709\u6240\u4e0d\u540c\u3002<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nExKine\u2122 Membrane and Cytoplasmic Protein Extraction kit<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Cytoplasmic Extraction Buffer (CEB) (5\u00d7)
\n\u2022 Membrane Extraction Buffer (MEB)
\n\u2022 Protease Inhibitor (100\u00d7)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Versatile\u2014suitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations.
\n\u2022 Fast and convenient\u2014non-denatured, active proteins are purified in less than two hours.
\n\u2022 Compatible\u2014apply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nPerform all steps at 2\u20138 \u00b0C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n

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\u819c&\u7d30\u80de\u8cea\u86cb\u767d\u63d0\u53d6\u8a66\u5291\u76d2 | ExKine\u2122 Membrane and Cytoplasmic Pro […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[4864],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33536"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33536"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33536\/revisions"}],"predecessor-version":[{"id":48204,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33536\/revisions\/48204"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33536"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33536"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33536"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}