{"id":33012,"date":"2022-06-29T16:00:29","date_gmt":"2022-06-29T08:00:29","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33012"},"modified":"2023-03-22T18:52:08","modified_gmt":"2023-03-22T10:52:08","slug":"purkine-his-tag-%e8%9b%8b%e7%99%bd%e7%b4%94%e5%8c%96%e8%a9%a6%e5%8a%91%e7%9b%92-ni-nta-purkine-his-tag-protein-purification-kit-ni-nta%e8%b2%a8%e8%99%9fktp2001","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=33012","title":{"rendered":"PurKine\u2122 His-Tag \u86cb\u767d\u7d14\u5316\u8a66\u5291\u76d2 (Ni-NTA) | PurKine\u2122 His-Tag Protein Purification Kit (Ni-NTA)\u8ca8\u865fKTP2001"},"content":{"rendered":"

\u9ad8\u54c1\u8cea\u86cb\u767d\u7d14\u5316\u6a39\u8102<\/span>\u00a0\u00a0<\/span>|\u00a0PurKine\u2122 His-Tag Ni-NTA Column<\/span><\/a><\/span><\/strong><\/span><\/p>\n

PurKine\u2122 His-Tag \u86cb\u767d\u7d14\u5316\u8a66\u5291\u76d2 (Ni-NTA) | PurKine\u2122 His-Tag Protein Purification Kit (Ni-NTA)\u8ca8\u865fKTP2001<\/a><\/span><\/strong><\/span><\/p>\n

\u00a050 mg\u00a0 6\u00d7His-tagged protein\/mL\u00a0 \u5e73\u5747\u73e0\u7c92\u5c3a\u5bf8\u70ba45-165 \u00b5m\u00a0 \u00a01ml\/1mlx5<\/span><\/p>\n

\u2022 PurKine\u2122 His-Tag Purification Nickel Column
\n\u2022 Phosphate-Buffered Saline (PBS,10X)
\n\u2022 Imidazole (2M)<\/p>\n

\"Fig.2.<\/p>\n

 <\/p>\n

PurKine\u2122 His-Tag \u7d14\u5316\u7cfb\u7d71(PurKine\u2122 His-Tag Purification system)\u57fa\u65bc\u5275\u65b0\u7684\u9ad8\u5bb9\u91cf IMAC \u57fa\u8cea(IMAC matrix)\uff0c\u53ef\u65b9\u4fbf\u5730\u5f9e\u7e3d\u88c2\u89e3\u7269(total lysates)\u4e2d\u4e00\u6b65\u7d14\u5316 His-tag \u86cb\u767d\u3002\u5c08\u6709\u7684\u96e2\u5b50\u87af\u5408\u5316\u5b78(ion-chelate chemistry)\u78ba\u4fdd\u8207\u5e38\u7528\u7684\u9084\u539f\u5291\uff08reducing agents, \u5982 DTT\uff09\u3001\u87af\u5408\u91d1\u5c6c\u86cb\u767d\u9176\u6291\u5236\u5291\uff08chelating metalloprotease inhibitors, \u5982 EDTA\uff09\u4ee5\u53ca\u5404\u7a2e\u7de9\u885d\u7269\u8cea(buffer substances)\u548c\u9e7d\u689d\u4ef6(salt conditions)\u5177\u6709\u975e\u51e1\u7684\u76f8\u5bb9\u6027\u3002\u8a72\u7522\u54c1\u7d44\u5408\u63d0\u4f9b\u591a\u7a2e\u87af\u5408\u57fa\u5718\uff08IDA\u3001NTA \u6216 Super NTA\uff09\u3001\u4ea4\u806f\u74ca\u8102\u7cd6\uff08\u4ea4\u806f 4% \u6216 6% \u74ca\u8102\u7cd6\uff09\u3001\u96e2\u5b50\uff08Nick\u3001Colbat\u3001\u9285\u6216\u975e\u5e36\u96fb\u96e2\u5b50\uff09\u548c\u517c\u5bb9\u6210\u5206\uff0c\u4ee5\u5be6\u73fe\u512a\u5316\u6700\u5927\u86cb\u767d\u8cea\u7522\u91cf\u3001\u7a69\u5b9a\u6027\u548c\u6eb6\u89e3\u5ea6\u7684\u904e\u7a0b\u3002\u6e2c\u8a66\u9084\u8b49\u5be6\uff0c\u5728\u81f3\u5c11\u4e94\u6b21\u91cd\u8907\u4f7f\u7528\u5f8c\u6027\u80fd\u6c92\u6709\u4e0b\u964d\u3002 PurKine\u2122 His-Tag \u7d14\u5316\u6a39\u8102\u4e5f\u53ef\u4f5c\u70ba\u9810\u88dd\u96e2\u5fc3\u67f1(prepacked spin column)\u548c\u8a66\u5291\u76d2(kit)\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

\"\"<\/p>\n

PurKine\u2122 His-Tag \u86cb\u767d\u7d14\u5316\u8a66\u5291\u76d2(PurKine\u2122 His-Tag Protein Purification Kit, KTP2001)\u53ef\u5728\u5404\u7a2e\u898f\u6a21\u7684\u91cd\u529b\u67f1(gravity column)\u7a0b\u5e8f\u4e2d\u6709\u6548\u7d14\u5316\u9ad8\u6c34\u5e73\u904e\u8868\u9054\u7684 His-tag \u878d\u5408\u86cb\u767d\u3002\u8a66\u5291\u76d2\u4e2d\u7684\u6a39\u8102(Resin) \u7531 90 \u03bcm \u9ad8\u5ea6\u4ea4\u806f\u7684 4% \u74ca\u8102\u7cd6\u73e0(highly cross-linked 4% agarose)\u7d44\u6210\uff0c\u8207\u785d\u9178\u4e09\u4e59\u9178 (Nitilotriacetic acid , NTA) \u5076\u806f\u3002\u7136\u5f8c\u87af\u5408\u57fa\u5718\u5e36\u6709\u93b3\u96e2\u5b50 (nickel ions , Ni2+)\u3002\u6e2c\u8a66\u8b49\u5be6\u5176\u6027\u80fd\u7b49\u65bc\u6216\u8d85\u904e\u5176\u4ed6\u4f9b\u61c9\u5546\u7684\u6d41\u884c Ni-NTA \u6a39\u8102(Ni-NTA resins)\uff0c\u4e26\u4e14\u5728\u81f3\u5c11\u4e94\u6b21\u91cd\u8907\u4f7f\u7528\u5f8c\u6027\u80fd\u6c92\u6709\u4e0b\u964d\u3002<\/p>\n

\"\"<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nPurKine\u2122 His-Tag Protein Purification Kit (Ni-NTA)<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nPurKine\u2122 His-Tag Protein Purification Kit effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin in kit consists of 90\u03bcm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nNi-NTA; His resin; Ni-NTA resin; His tag resin<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 PurKine\u2122 His-Tag Purification Nickel Column
\n\u2022 Phosphate-Buffered Saline (PBS,10X)
\n\u2022 Imidazole (2M)<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 High capacity\u2014More than 50 mg of 6xHis-tagged protein per milliliter of resin.
\n\u2022Versatile\u2014For purification under native or denaturing conditions.
\n\u2022 Cost-effective\u2014No performance decrease after at least five repeated uses of the same batch of resin.
\n\u2022 Flexible\u2014available in multiple formats including bulk resin, spin columns and complete kits.
\n\u2022 Easy to use\u2014 Pre-formulated buffers available for kit formats.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at 2-8\u00b0C from date of shipment. Do not freeze.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nBlue ice<\/td>\n<\/tr>\n
kground<\/strong><\/td>\nPurKine\u2122 His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine\u2122 His-Tag Purification resin is also available as prepacked spin column and kit formats.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nNi-NTA; His resin; Ni-NTA resin; His tag resin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Sample Preparation <\/span><\/strong><\/span><\/p>\n

\u2160 Protein extraction from bacteria or yeast<\/span><\/strong><\/span><\/p>\n

1. Transfer bacteria or yeast culture medium to centrifugal tube after induced protein expression, centrifuge 15 min at 7,000 rpm to collect bacteria or yeast. 2. Mix with 10 times volume of Lysis buffer (collected bacteria or yeast:Lysis buffer=1:10), add PMSF with final concentration 1mM. Lysozyme is recommended to be added with a final concentration of 0.2-0.4 mg\/mL. 3. Resuspend the cells. Add 10 \u03bcg\/ml RNase A and 5\u03bcg\/ml DNase I in case the cells\u2019 concentration is high. Mix and sonicate the suspension in ice, keep the lystes to be clean. 4. Transfer the clean lysate to a new centrifuge tube, centrifuge for 20-30 min at 10,000 rpm, 4\u2103. Take the supernatant, store on ice or -20\u2103.<\/p>\n

\u2161 Soluble protein extraction from yeast, insect (\u6606\u87f2) or mammalian cells <\/span><\/strong><\/span><\/p>\n

1. Transfer cell culture medium to centrifugal tube, centrifuge 10 min at 5,000 rpm to collect suspension. Suspension should be dialyzed with Lysis buffer in case there\u2019re EDTA, histidine and other reductants in suspension.<\/p>\n

2. Large volume of suspension should be concentrated by Ammonium sulfate fractional precipitation method, following be dialyzed with Lysis buffer.<\/p>\n

\u2162 Inclusion-body protein extraction for purification in denaturing conditions <\/span><\/strong><\/p>\n

1. Transfer culture medium to centrifugal tube, centrifuge 15 min at 7,000 rpm to collect precipitation. Remove the suspension.<\/p>\n

2. Mix with 10 times volume of Lysis buffer, such as collected precipitation: Lysis buffer= 1:10 (W\/V). Lysis buffer does not contain 8 M Urea. Resuspend the precipitation. Mix and sonicate the suspension in ice.<\/p>\n

3. Transfer the lysate to a new centrifuge tube, centrifuge for 20-30 min at 10,000 rpm, 4\u2103. Dispose the supernatant, Repeat step 2 and 3 once again.<\/p>\n

4. Mix with 10 times volume of Lysis buffer, such as collected precipitation: Lysis buffer=1:10 (W\/V). Resuspend the lysate for purification in denaturing conditions.<\/p>\n

Reagent Preparation<\/strong><\/span><\/p>\n

It is recommended to filter all buffers before use by passing through a 0.22 \u03bcm or 0.45 \u03bcm filter. For most proteins, the following buffer are recommended:<\/p>\n

Note: Sometimes overexpressed proteins are sequestered in inclusion bodies. Inclusion bodies of His-tagged proteins can be solubilized in 8M urea or 6M guanidine. We recommend you use the buffer below (Reagents below are not supplied in the kit)\uff1a For imidazole elution method prepare the following buffers: Lysis buffer: 8 M Urea, 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0; Wash buffer: 8 M Urea, 50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, pH 8.0; Elution buffer: 8 M Urea, 50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0. For pH elution method prepare the following buffers: Lysis buffer: 8 M Urea, 100 mM NaH2PO4, 100 mM Tris\u00b7HCl, pH 8.0; Wash buffer: 8 M Urea, 100 mM NaH2PO4, 100 mM Tris\u00b7HCl, pH 6.3; Elution buffer: 8 M Urea, 100 mM NaH2PO4, 100 mM Tris\u00b7HCl, pH 4.5.<\/p>\n

Procedure for Sample Purification <\/span><\/strong><\/span><\/p>\n

1. Fix Column. Move the top and bottom stopper, and let the storage buffer drain away.<\/p>\n

2. Add 5 resin-bed volume Lysis Buffer to the column. Equilibrate the column (make the Ni-NTA Resin in the same buffer system as the target protein to protect the protein). Allow buffer to drain from the column.<\/p>\n

3. Add the prepared protein extract to the resin. (In order to improve the recovery rate of the target protein, the adding speed was controlled to ensure the full contact between the target protein and Ni2+). Note: Collect the flow-through which can be analyzed by SDS-PAGE. When problems arise, it is easier to find solutions.<\/p>\n

4. Add 10-15 resin-bed volume Wash Buffer to the column to remove the non-specific adsorption protein. Pay attention to collecting the flow-through.<\/p>\n

5. Add 5-10 resin-bed volume Elution Buffer to the column to wash the target protein. The collected eluate is the target protein solution.<\/p>\n

6. Add 3 resin-bed volume Lysis Buffer and 5 resin-bed volume deionized water to the column in turn to equilibrate the Ni-NTA Resin. Store resin in an equal volume of PBS containing 20% ethanol at 4-30\u2103 to prevent the resin from being contaminated by bacteria.<\/p>\n

7. The flow-through, eluted protein and prepared protein extract can be directly analyzed by SDS-PAGE to test the purification effect.<\/p>\n

Cleaning-in-Place (CIP) <\/span><\/strong><\/span><\/p>\n

When the back pressure is too high or obvious contamination appears on the resin during the use of the resin, it needs to be cleaned-in-place (CIP). It is recommended to follow the steps below to remove residual contaminants on the resin, such as precipitated proteins, hydrophobins, and lipoproteins.<\/p>\n

To remove strongly bound hydrophobic proteins, lipoproteins and lipids: Wash the column using 5-10 resin-bed volumes of 30% isopropanol contacting for 15-20 min. Or apply 2 resin-bed volumes of acidic or alkaline solution containing detergent (i.e. 0.1 M acetic acid solution contains 0.1-0.5% non-ionic detergent), for 1-2 hours. Finally wash the column with 10 resin-bed volumes of distilled water.<\/p>\n

To remove the proteins engaged with ionic interaction: Wash the column with 1.5 M NaCl for 10-15 min. Finally wash the column with 10 resin-bed volumes of distilled water.<\/p>\n

Ni-NTA Resin Regeneration <\/span><\/strong><\/span><\/p>\n

In general, The Ni-NTA resin may be used at least five times before it becomes necessary to recharge them with metal ions. When the back pressure is too high or the capacity significantly lower, it needs to strip the metal ions and recharge the resin as the following procedure:<\/p>\n

1. Wash resin with 5 resin-bed volumes of deionized water;<\/p>\n

2. 100 mM EDTA (pH 8.0), 5 resin-bed volumes;<\/p>\n

3. Wash resin with 10 resin-bed volumes of deionized water;<\/p>\n

4. Wash resin with 5 resin-bed volumes of 0.5 M NaOH and stay for 10-15 min;<\/p>\n

5. Wash resin with 10 resin-bed volumes of deionized water;<\/p>\n

6. 100mM NiSO4, 3-5 resin-bed volumes;<\/p>\n

7. Wash resin with 10 resin-bed volumes of deionized water; After regeneration, the medium can be used immediately or store in PBS containing 20% ethanol at 4\u2103.<\/p>\n

 <\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

BRAND\/AGENCY\/\u53f0\u7063\u4ee3\u7406\u5546<\/span>\/\u00a0<\/span>Abbkine\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/span><\/strong><\/h2>\n

\"abbkine\u5546\u54c1\u63d2\u5716\"\"\u539f\u5ee0\u9023\u7d5001\"<\/a><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

https:\/\/www.abbkine.com\/<\/p>\n

Abbkine Scientific Co., Ltd\u7531\u4e00\u7fa4\u5fd7\u540c\u9053\u5408\u7684\u79d1\u5b78\u5bb6\u548c\u884c\u92b7\u5c08\u5bb6\uff0c\u57282012\u5e74\u6210\u7acb\u65bc\u7f8e\u570b\u52a0\u5229\u798f\u5c3c\u4e9e\u5dde\uff0c\u96a8\u8457\u4e9e\u6d32\u5e02\u5834\u7684\u8fc5\u731b\u589e\u9577\uff0cAbbkine\u5c07\u7e3d\u90e8\u642c\u9077\u81f3\u4e2d\u570b\u3002\u7d50\u5408\u4e86Abbkine\u7f8e\u570b\u6280\u8853\u548c\u4e2d\u570b\u88fd\u9020\u5169\u65b9\u9762\u7684\u5de8\u5927\u512a\u52e2\uff0cAbbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\uff0c\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002<\/p>\n

\u767c\u5c55\u6b77\u7a0b<\/span><\/strong><\/p>\n

Abbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002Abbkine\u4ee5\u7279\u8272\u7684\u86cb\u767d\u8cea\u751f\u7522\u8207\u6aa2\u6e2c\u8a66\u5291\u8d77\u5bb6\uff0c\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7814\u767c\u5404\u985e\u6297\u9ad4\u3001\u751f\u5316\u8a66\u5291\u3001\u86cb\u767d\u8cea\u548c\u5be6\u9a57\u8a66\u5291\u76d2\uff0c\u4ee5\u671f\u671b\u70ba\u5168\u7403\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u9769\u65b0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u5f88\u81ea\u8c6a\u5730\u70ba\u5ba2\u6236\u63d0\u4f9b\u5982\u4e0b\u7279\u8272\u7522\u54c1\u7dda\uff0c\u4e26\u6703\u4e00\u5982\u65e2\u5f80\u5730\u958b\u767c\u65b0\u578b\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u8a3a\u65b7\u8a66\u5291\u7522\u54c1\u30022017\u5e74\u6aa2\u6e2c\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u7684ELISA\u8a66\u5291\u76d2\u61c9\u904b\u800c\u751f: \u6839\u64da\u5e02\u5834\u9700\u6c42\uff0c\u958b\u767cEliKine\u2122\u7cfb\u5217ELISA\u8a66\u5291\u76d2\uff0c\u6db5\u84cb\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u71b1\u9580\u6307\u6a19\uff0c\u5982\u767d\u4ecb\u7d20\uff08IL\uff09\u3001\u816b\u7624\u58de\u6b7b\u56e0\u6578\uff08TNF\uff09\u3001\u5e72\u64fe\u7d20\uff08IFN\uff09\u7b49\u660e\u661f\u7d1a\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\u5168\u7403\u767c\u552e\u30022018\u5e74\u6191\u85c9\u5148\u9032\u7684\u86cb\u767d\u8cea\u5076\u806f\u6280\u8853\u548c\u56b4\u683c\u7684\u54c1\u8cea\u63a7\u5236\uff0c\u6211\u5011\u63d0\u4f9bLinKine\u2122\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\uff0c\u5728\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u591a\u80bd\u6216\u8005\u5176\u5b83\u751f\u7269\u5206\u5b50\u4e0a\u53ef\u76f4\u63a5\u6a19\u8a18HRP\uff0cBiotin\uff0cFITC\uff0cCy3\uff0cAbFluor\u2122\u4ee5\u6eff\u8db3\u79d1\u7814\u61c9\u7528\uff0c\u85e5\u7269\u7814\u767c\u4ee5\u53ca\u8a3a\u65b7\u8a66\u5291\u76d2\u958b\u767c\u7684\u9700\u6c42\u30022019\u5e74\u751f\u5316\u3001\u7d30\u80de\u985e\u7522\u54c1\u7dda\u9010\u6b65\u64f4\u5145\u642d\u5efa\u7d30\u80de\u7522\u54c1\u7814\u767c\u5e73\u81fa\uff0c\u7d30\u80de\u72c0\u614b\u6aa2\u6e2c\u3001\u7d30\u80de\u67d3\u8272\u3001\u7d30\u80de\u63d0\u53d6\u3001\u751f\u5316\u8a66\u5291\u76d2\u7522\u54c1\u5f97\u5230\u8d8a\u4f86\u8d8a\u591a\u5168\u7403\u5ba2\u6236\u7684\u8a8d\u53ef\u3002<\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

\u7d30\u80de\u589e\u6b96&\u6bd2\u7406&\u8870\u8001 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
\u8ca8\u865fKTA1001<\/a><\/span><\/td>\n\u6d3b&\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/span><\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/span><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/span><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n1000\u500b\u53cd\u61c9<\/span><\/td>\n\u5438\u6536\u5149450nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/span><\/td>\nEdU Image\u7d30\u80de\u589e\u6b96\u8a66\u5291\u76d2<\/a>\u00a0(BrdU\u66ff\u4ee3\u6cd5)<\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1110<\/a><\/span><\/td>\n\uff08LDH\uff09\u4e73\u9178\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

\u03bbmax= 450 nm<\/span><\/p>\n

1- 20 U\/mL of LDH<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/span><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em=535\/615 nm.<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/span><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

\u00a0OD 565 nm<\/span><\/p>\n

10,00-100,0000 cells per well<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/span><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/span><\/td>\n50ug<\/span><\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/span><\/p>\n

\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\u7d30\u80de\u51cb\u4ea1&\u7d30\u80de\u67d3\u8272 (8)<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/span><\/p>\n

\u00a0<\/span><\/td>\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/span><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/span><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/span><\/p>\n

JC-1 Stain<\/span><\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/span><\/td>\nDAPI Staining Solution<\/a><\/span><\/td>\n10MG<\/span><\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/span><\/td>\n<\/tr>\n
\u00a0KTD102-EN<\/a><\/span><\/td>\n\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5)<\/a><\/span><\/td>\n40\u500b\u5206\u6790\/20\u500b\u5206\u6790<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/span><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 500\/520 nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/span><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 484\/501 nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/span><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

DNA Damege\u640d\u50b7\/\u7d30\u80deStress (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1050<\/a><\/span><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD 532 nm<\/span><\/p>\n

1-100 \u00b5M\/L<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1910<\/a><\/span><\/td>\n\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nDCFH-DA (10 mM)<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/span><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

colorimetric (540 nm)<\/span><\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/span><\/td>\n<\/tr>\n
\u00a0\u8ca8\u865f<\/u><\/strong>\u00a0KTB1091<\/a><\/span><\/td>\n\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\u5438\u6536\u5149 520nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1041<\/a><\/span><\/td>\nCheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

480\u500b\u53cd\u61c9<\/span><\/td>\n

\n

OD 580 nm<\/span><\/p>\n

: 1 \u00b5M-100 \u00b5M<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1210<\/a><\/span><\/td>\nCheKine\u2122 \u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

absorption peak at 540nm<\/span><\/p>\n

0.0125-0.5 mg\/mL<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1020<\/a><\/span><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD565 nm<\/span><\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6297\u6c27\u5316\u5206\u6790\u8a66\u5291 (8)<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1030<\/a><\/span><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

200-3.13 U\/ml<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/span><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD 540 nm<\/span><\/p>\n

2-75 \u00b5M<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/span><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTCA\u5206\u6790<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

480\u500b\u53cd\u61c9<\/span><\/td>\n

\n

593 nm<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/span><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

240nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/span><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

412 nm \u00a02-200 \u00b5g\/mL<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB3010<\/a><\/span><\/td>\nCheKine\u2122 \u82b1\u9752\u7d20\u9084\u539f\u9176\uff08ANR\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\nabsorption at 340 nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1530<\/a><\/span><\/td>\n\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

absorption peak at 502 nm<\/span><\/p>\n

0.156-10 mg\/g<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1140<\/a><\/span><\/td>\n\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

\u00a0absorption peak at 525 nm<\/span><\/p>\n

\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u81bd\u56fa\u9187\u4ee3\u8b1d\/\u4ee3\u8b1d\u8a66\u9a57 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/span><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/span><\/p>\n

Standard: 5 \u03bcmol\/mL.<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/span><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

\u00a0\u5438\u5149\u503c630nm<\/span><\/p>\n

4- 300 mg\/dL)<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1340<\/a><\/span><\/td>\nCheKine\u2122 \u809d\u91a3\/\u7cd6\u539f<\/u><\/strong>\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/span><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

500nm Standard: 5 \u03bcmol\/mL<\/span><\/p>\n

\u00a0<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB2250<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (HDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

Absorption peak at 500nm<\/span><\/p>\n

Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB2260<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (LDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

\n

Absorption peak at 500nm<\/span><\/p>\n

Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1390<\/a><\/span><\/td>\nCheKine\u2122 \u6fb1\u7c89\u5206\u652f\u9176 (SBE) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

absorption at 660 nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD 450 nm<\/span><\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u751f\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/span><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\u5438\u6536\u5149510 nm<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1542<\/a><\/span><\/td>\nTannase(TAN) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

absorbance at 270 nm<\/span><\/p>\n

0.3125-20 \u03bcmoL\/mL<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1541<\/a><\/span><\/td>\nCheKine\u2122 \u55ae\u5be7\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Tannin Colorimetric Assay Kit<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

absorbance at 760 nm<\/span><\/p>\n

0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1290<\/a><\/span><\/td>\nCheKine\u2122 \u70cf\u982d\u9178\u9176 (ACO) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\nabsorption at 340 nm.<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTB1560<\/a><\/span><\/td>\n\u9187\u91af\u57fa\u8f49\u79fb\u9176\uff08AAT\uff09\u6d3b\u6027\u6bd4\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/span><\/p>\n

96\u500b\u53cd\u61c9<\/span><\/td>\n

absorption at 412 nm.<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD 450 nm<\/span><\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n

\u8ca8\u865fKTB1380<\/a><\/span><\/td>\n\u03b2-\u6fb1\u7c89\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

absorption peak at 540 nm<\/span><\/p>\n

0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1310<\/a><\/span><\/td>\n\u8461\u8404\u7cd6\u6c27\u5316\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD 580 \u4e0b\u6e2c\u91cf<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a00.05 – 2 U\/L<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u767c\u708e\u53cd\u61c9ELISA\u5206\u6790<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/span><\/td>\nIL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

\n

450 nm<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/span><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

\n

450 nm<\/span><\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/span><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

\n

450 nm<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/span><\/td>\n<\/tr>\n

KET7013<\/span><\/td>\nIL-22 ELISA\u8a66\u5291\u76d2<\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7003<\/a><\/span><\/td>\nIFN-\u03b3\uff08\u5e72\u64fe\u7d20-\u03b3\uff09ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

31.25 pg\/ml- 2000 pg\/ml<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKET6019<\/a><\/span><\/td>\nIL-10 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

\u00a0<\/span><\/td>\n

2.35 pg\/ml-150 pg\/ml<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKET7002<\/span><\/td>\nGM-CSF ELISA\u8a66\u5291\u76d2<\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n7.8 pg\/mL-500 pg\/mL<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/span><\/td>\n\u767d\u4ecb\u7d20-1\u03b2ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/span><\/p>\n

96 X5\u76e4<\/span><\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u91cd\u7d44\u86cb\u767d&\u55ae\u591a\u682a\u6297\u9ad4<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>PRP100273<\/a><\/span><\/td>\nHuman IL-4 protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n20ug<\/span><\/td>\nED50 for this effect is 0.05-0.2 ng\/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 \u00d7 10E4 IU\/\u03bcg.<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>PRP100159<\/a><\/span><\/td>\nHuman EGF protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n100ug<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>ABP52783<\/a><\/span><\/td>\nGAPDH Polyclonal Antibody<\/a><\/span><\/td>\n30ul<\/span><\/td>\nHuman, Mouse, Rat\u3002\u53ef\u61c9\u7528\u65bcELISA, IHC-P, WB\u5be6\u9a57\u3002<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fA21010<\/span><\/td>\nHRP, Goat Anti-Mouse IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fA21020<\/span><\/td>\nHRP, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fA23220<\/span><\/td>\nDylight 488, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6297\u9ad4\u86cb\u767d\u7d14\u5316&\u5176\u5b83<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTP2070<\/a><\/span><\/td>\n\u6297\u9ad4\u7d14\u5316\u8a66\u5291\u76d2\uff08\u86cb\u767d A\/G\uff09<\/a><\/span><\/td>\n1ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fBMR2001<\/a><\/span><\/td>\nHis-Tag Ni-NTA Resin\u6a39\u8102<\/a><\/span><\/td>\n5ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>\u00a0KTD105-EN<\/a><\/span><\/td>\nIP\/Co-IP Toolkit (Agarose)\u00a0<\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fKTD3001<\/a><\/span><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/span><\/td>\n500\u500b\u53cd\u61c9<\/span><\/td>\n\n

OD 562 nm<\/span><\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/span><\/p>\n

50 to 1000 \u00b5g\/ml.<\/span><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTP2140<\/a><\/span><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)<\/a><\/span><\/td>\n1ml\u00d75<\/span><\/td>\nMore than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/span><\/td>\n<\/tr>\n
BMD0062<\/span><\/td>\nHoechst 33342<\/span><\/td>\n10MG<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
BMU104-EN<\/span><\/td>\n\u589e\u5f37\u578bAntifade\u5c01\u7247\u5291<\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMS0050<\/a><\/span><\/td>\n\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05<\/a><\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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