{"id":32947,"date":"2022-06-22T14:46:43","date_gmt":"2022-06-22T06:46:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32947"},"modified":"2022-07-19T11:11:20","modified_gmt":"2022-07-19T03:11:20","slug":"%e5%b1%b1%e7%be%8a%e6%8a%97%e5%b0%8f%e9%bc%a0-lambda-hrp-%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f1060-05","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=32947","title":{"rendered":"\u5c71\u7f8a\u6297\u5c0f\u9f20 Lambda-HRP \u6297\u9ad4 | \u8ca8\u865f1060-05"},"content":{"rendered":"

\u5c71\u7f8a\u6297\u5c0f\u9f20 Lambda-HRP \u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>1060-05<\/strong><\/a><\/span> |<\/strong>\u898f\u683c<\/strong>1ml<\/strong><\/p>\n

Goat Anti-Mouse Lambda-HRP antibody for use in ELISA, immunohistochemistry \/ immunocytochemistry, western blot, and ELISpot assays.<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>: <\/strong>\u5c71\u7f8a\u6297\u5c0f\u9f20 Lambda-HRP \u6297\u9ad4\uff0c\u7528\u65bc ELISA\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b78\/\u514d\u75ab\u7d30\u80de\u5316\u5b78\u3001\u86cb\u767d\u8cea\u5370\u8de1\u548c ELISpot \u6e2c\u5b9a\u3002\u5c0dmouse \u03bb light chains\u5177\u6709\u5c08\u4e00\u6027\uff0c\u4f86\u6e90\u70baPooled antisera from goats hyperimmunized with mouse \u03bb light chains\u3002\u4ea4\u53c9\u5438\u9644(Cross Adsorption<\/strong> ) Mouse \u03ba light chains; may react with \u03bb light chains from other species\u3002\u7de9\u885d\u6db2\u70ba50% Glycerol\/50% Phosphate buffered saline, pH 7.4\u3002<\/p>\n

\"\"<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Isotype<\/strong><\/td>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\n0109-05<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nReacts with mouse \u03bb light chains<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from goats hyperimmunized with mouse \u03bb light chains<\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nMouse \u03ba light chains; may react with \u03bb light chains from other species<\/td>\n<\/tr>\n
Purification Method<\/strong><\/td>\nAffinity chromatography on mouse \u03bb light chains covalently linked to agarose<\/td>\n<\/tr>\n
Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Clonality<\/strong><\/td>\nPolyclonal<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\nLot specific<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Recommended Storage<\/strong><\/td>\n2-8\u00b0C<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nQuality tested applications for relevant formats include –
\nELISA\u00a01-6,23<\/sup>
\nFLISA
\nFlow Cytometry\u00a03,5,7-13,24<\/sup>Other referenced applications for relevant formats include –
\nELISpot\u00a06,7<\/sup>
\nImmunohistochemistry-Frozen Sections\u00a02,13-16<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a017,23<\/sup>
\nImmunocytochemistry\u00a01,4,18<\/sup>
\nWestern Blot\u00a019,20,25<\/sup>
\nImmunoprecipitation\u00a021,22<\/sup>
\nStimulation\u00a023,24<\/sup>
\nSeparation\u00a025<\/sup><\/td>\n<\/tr>\n
RRID Number<\/strong><\/td>\nAB_2794393<\/td>\n<\/tr>\n
Gene ID<\/strong><\/td>\n111519<\/a>\u00a0(Mouse)<\/td>\n<\/tr>\n
Gene ID Symbol<\/strong><\/td>\nIgl<\/a>\u00a0(Mouse)<\/td>\n<\/tr>\n
Gene ID Aliases<\/strong><\/td>\nIgl-x<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6587\u737b\u8cc7\u6599<\/p>\n

1. Gollahon KA, Hagman J, Brinster RL, Storb U. Ig \u03bb-producing B cells do not show feedback inhibition of gene rearrangement. J Immunol. 1988;141:2771-80. (ELISA, ICC)<\/p>\n

2. Mandik-Nayak L, Nayak S, Sokol C, Eaton-Bassiri A, Madaio MP, Caton AJ, et al. The origin of anti-nuclear antibodies in bcl-2 transgenic mice. Int Immunol. 2000;12:353-64. (ELISA, IHC-FS)<\/p>\n

3. Shivtiel S, Leider N, Melamed D. Receptor editing in CD45-deficient immature B cells. Eur J Immunol. 2002;32:2264-73. (ELISA, FC)<\/p>\n

\u7d30\u80de\u514d\u75ab\u5c08\u5bb6<\/strong>\u6975\u529b\u63a8\u85a6<\/strong>SouthernBiotech<\/strong>\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\u3002\u7269\u7a2e\u7a2e\u985e\u9f4a\u5168\uff0c\u5305\u62ec\u6709Bovine\u3001Canine\u3001Chicken\u3001Cynomolgus\u3001Donkey\u3001Equine\u3001Feline\u3001Goat\u3001Guinea Pig\u3001Hamster\u3001Human\u3001Monkey\u3001Mouse\u3001Porcine\u3001Rabbit\u3001Rat\u3001Rhesus\u3001Sheep\u3001Turkey\u3002\u5177\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001PE\u3001APC\u3001SPRD\u3001PE\/CY5.5\u3001APC\/CY5.5\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u5404\u7a2e\u87a2\u5149\u6a19\u8a8c\u3001\u53e6\u5177\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001\u6216\u672a\u6a19\u8a8c\u53caLow Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\u3002<\/p>\n

SouthernBiotech \u6297\u5c0f\u9f20\u4e8c\u6297(anti-mouse secondary antibodies)\u662f\u7d93\u904e\u5ee3\u6cdb\u8868\u5fb5\u7684\u89aa\u548c\u7d14\u5316(affinity-purified)\u7684\u591a\u514b\u9686(polyclonal)\u6216\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibodies)\uff0c\u5c0d\u5c0f\u9f20\u514d\u75ab\u7403\u86cb\u767d\u91cd\u93c8\u985e\u5225(mouse immunoglobulin heavy chain classes)\u548c\u4e9e\u985e(subclasses)\u3001\u8f15\u934a(light chains)\u548c\u7247\u6bb5(fragments)\u5177\u6709\u7279\u7570\u6027(specificities)\u3002SouthernBiotech\u63d0\u4f9b\u5728\u5c71\u7f8a(goat)\u3001\u5154(rabbit)\u3001\u9a62(donkey)\u548c\u5927\u9f20(rat)\u4e2d\u7522\u751f\u7684\u6297\u5c0f\u9f20\u4e8c\u6297\u3002\u5b83\u5011\u5728\u6aa2\u6e2c\u4e00\u6297(primary antibodies<\/a>\u00a0)\u65b9\u9762\u5f88\u6709\u50f9\u503c\uff0c\u4e26\u4e14\u6709\u591a\u7a2e\u5f62\u5f0f\u53ef\u4f9b\u9078\u64c7\uff0c\u5305\u62ec\u672a\u5076\u806f\u4ee5\u53ca\u9176\u3001\u751f\u7269\u7d20\u5316\u548c\u87a2\u5149\u5076\u806f\u7269\uff0c\u7528\u65bc ELISA\u3001\u86cb\u767d\u8cea\u5370\u8de1(western blot)\u3001\u6d41\u5f0f\u7d30\u80de\u8853(flow cytometry)\u3001\u591a\u91cd\u548c\u514d\u75ab\u7d44\u7e54\u5316\u5b78(immunohistochemistry)\u7b49\u5206\u6790\u3002<\/p>\n

\u7531\u65bc\u55ae\u514b\u9686\u6297\u9ad4 (\u00a0monoclonal antibodies\u00a0<\/a>)\u5c07\u7d50\u5408\u6297\u9ad4\u9776\u6a19\u4e0a\u7684\u55ae\u500b\u8868\u4f4d\uff0c\u56e0\u6b64\u5b83\u5011\u5177\u6709\u9ad8\u5ea6\u7279\u7570\u6027(specific)\uff0c\u53ef\u63d0\u4f9b\u51fa\u8272\u7684\u6279\u6b21\u9593\u4e00\u81f4\u6027(lot-to-lot consistency)\uff0c\u4e26\u4e14\u901a\u5e38\u5728\u6587\u737b\u4e2d\u5f97\u5230\u5f88\u597d\u7684\u8868\u5fb5\u3002\u6211\u5011\u7684\u591a\u514b\u9686\u6297\u5c0f\u9f20\u4e8c\u6297(polyclonal anti-mouse secondary antibodies)\u53ef\u4ee5\u901a\u904e\u4fe1\u865f\u653e\u5927\u63d0\u9ad8\u9748\u654f\u5ea6(sensitivity)\uff0c\u56e0\u70ba\u591a\u500b\u4e8c\u6297\u53ef\u4ee5\u8207\u55ae\u500b\u4e00\u6297\u7d50\u5408\u3002<\/p>\n

\u9078\u5b9a\u7684\u6297\u9ad4\u5df2\u8207\u4f86\u81ea\u5176\u4ed6\u7269\u7a2e\u7684 IgG \u548c\/\u6216\u8840\u6e05\u86cb\u767d\u4ea4\u53c9\u5438\u9644\uff0c\u4ee5\u7522\u751f\u5c0d\u9019\u4e9b\u7269\u7a2e\u5177\u6709\u6700\u5c0f\u4ea4\u53c9\u53cd\u61c9\u6027(cross-reactivity)\u7684\u6297\u9ad4\u3002\u4f7f\u7528\u5c0d\u60a8\u7684\u6a23\u54c1\u7269\u7a2e\u5177\u6709\u6700\u5c0f\u4ea4\u53c9\u53cd\u61c9\u6027\u7684\u4e8c\u6297\u975e\u5e38\u91cd\u8981\u3002\u901a\u904e\u63d0\u4f9b\u5168\u9762\u7684\u591a\u514b\u9686(polyclonal)\u548c\u55ae\u514b\u9686(monoclonal)\u6297\u5c0f\u9f20\u985e(anti-mouse class)\u548c\u4e9e\u985e\u7279\u7570\u6027\u4e8c\u6297\uff08\u4f8b\u5982 IgG1\u3001IgG2b\u3001IgM\uff09\uff0c\u53ef\u4ee5\u5728\u6e2c\u5b9a\u4e2d\u540c\u6642\u6aa2\u6e2c\u591a\u7a2e\u5c0f\u9f20\u4e00\u6297\u3002\u6211\u5011\u6709\u5ee3\u6cdb\u7684\u5168\u5206\u5b50\u548cF(ab\u2019)2<\/sub>\u00a0fragment<\/a>\u00a0\u7247\u00a0\u6bb5\u591a\u514b\u9686\u548c\u55ae\u514b\u9686\u6297\u5c0f\u9f20\u6297\u9ad4\u53ef\u4f9b\u9078\u64c7\uff0c\u6240\u6709\u9019\u4e9b\u6297\u9ad4\u90fd\u662f\u5167\u90e8\u751f\u7522\u548c\u9a57\u8b49\u7684\uff0c\u53ef\u7528\u65bc\u60a8\u7684\u7279\u5b9a\u6aa2\u6e2c\u3002<\/p>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"Isotype control <\/strong>\u5206\u578b\u5c0d\u7167\u7d44<\/strong><\/a> | <\/strong>\u5206\u578b\u5c0d\u7167\u7d44\u5957\u7d44<\/strong><\/a> | <\/strong>\u6297\u9ad4\u5206\u578b<\/strong>ELISA <\/strong>\u5957\u7d44<\/strong><\/a> | <\/strong>anti-rabbit <\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong>anti-mouse<\/strong>\u4e8c\u7d1a\u6297\u9ad4<\/strong><\/a> | <\/strong><\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5c71\u7f8a\u6297\u5c0f\u9f20 Lambda-HRP \u6297\u9ad4 | \u8ca8\u865f1060-05 |\u898f\u683c1ml Goat Anti-Mouse […]<\/p>\n","protected":false},"author":3,"featured_media":29305,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[841],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32947"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32947"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32947\/revisions"}],"predecessor-version":[{"id":32949,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32947\/revisions\/32949"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29305"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32947"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32947"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}