{"id":31681,"date":"2022-03-31T18:22:28","date_gmt":"2022-03-31T10:22:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31681"},"modified":"2024-05-06T10:36:41","modified_gmt":"2024-05-06T02:36:41","slug":"%e7%a1%9d%e9%85%b8%e9%b9%bd%e9%82%84%e5%8e%9f%e9%85%b6%e5%be%ae%e5%ad%94%e6%9d%bf%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-nitrate-reductase-microplate-assay-kit-bioworld%e8%b2%a8%e8%99%9fask101","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=31681","title":{"rendered":"\u785d\u9178\u9e7d\u9084\u539f\u9176\u5fae\u5b54\u677f\u6aa2\u6e2c\u8a66\u5291\u76d2 | Nitrate Reductase Microplate Assay Kit BioWorld\u8ca8\u865fASK1014"},"content":{"rendered":"

\u785d\u9178\u9e7d\u9084\u539f\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 | Nitrate Reductase Microplate Assay Kit BioWorld\u8ca8\u865fASK1014<\/a><\/span><\/strong><\/p>\n

\"\"<\/p>\n

\u785d\u9178\u9084\u539f\u9176\u6d3b\u6027\u53ef\u4f5c\u70ba\u9810\u6e2c\u7a40\u7269\u7684\u751f\u5316\u5de5\u5177\u7522\u91cf\u548c\u7a40\u7269\u86cb\u767d\u8cea\u7522\u91cf\u3002\u785d\u9178\u9084\u539f\u9176\u4fc3\u9032\u6c28\u57fa\u9178\u8336\u8449\u751f\u7522\u3002\u64da\u6089\uff0c\u8336\u6a39\u5674\u7051\u5404\u7a2e\u5fae\u91cf\u71df\u990a\u7d20\uff08\u5982 Zn\u3001Mn \u548c B\uff09\u8207 Mo \u4e00\u8d77\u63d0\u9ad8\u4e86\u6c28\u57fa\u9178\u542b\u91cf\u8336\u82bd\u548c\u4f5c\u7269\u7522\u91cf\u3002\u8a72\u6e2c\u5b9a\u4ee5\u785d\u9178\u9e7d\u5c0d\u785d\u9178\u9e7d\u7684\u9176\u4fc3\u6c34\u89e3\u958b\u59cb\u9084\u539f\u9176\u3002\u9176\u50ac\u5316\u53cd\u61c9\u7522\u7269\u5076\u6c2e\u5316\u5408\u7269\u53ef\u6e2c\u5b9a\u5728 540 nm \u6bd4\u8272\u8b80\u6578\u3002<\/p>\n

Nitrate Reductase (NR), also known as respiratory nitrate reductase. Eukaryotic nitrate reductases are part of the sulfite oxidase family of molybdoenzymes. They transfer electrons from NADH or NADPH to nitrate. Prokaryotic nitrate reductases belong to the DMSO reductase family of molybdoenzymes and have been classified into three groups, assimilatory nitrate reductases (Nas), respiratory nitrate reductase (Nar), and periplasmic nitrate reductases (Nap). The active site of these enzymes is a Mo ion that is bound to the four thiolate functions of two pterin molecules. The coordination sphere of the Mo is completed by one amino-acid side chain and oxygen and\/or sulfur ligands. The exact environment of the Mo ion in certain of these enzymes (oxygen versus sulfur as a sixth molybdenum ligand) is still debated. The Mo is covalently attached to the protein by a cysteine ligand in Nap, and an aspartate in Nar. Nitrate reductase activity can be used as a biochemical tool for predicting grain yield and grain protein production. Nitrate reductase promotes amino acid production in tea leaves. It is reported that tea plants sprayed with various micronutrients (like Zn, Mn and B) along with Mo enhanced the amino acid content of tea shoots and also the crop yield. The assay is initiated with the enzymatic hydrolysis of the nitrate by Nitrate Reductase. The enzyme catalysed reaction products azo-compound can be measured at a colorimetric readout at 540 nm.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/strong><\/td>\nNitrate Reductase Microplate Assay Kit<\/td>\n<\/tr>\n
Catalog No.<\/strong><\/td>\nASK1014<\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFunctional Assay<\/td>\n<\/tr>\n
Description<\/strong><\/td>\nDetection and Quantification of Nitrate Reductase Activity.<\/td>\n<\/tr>\n
Alternative Names<\/strong><\/td>\nEC 1.7.99.4; Nitrate reductase; Respiratory nitrate reductase<\/td>\n<\/tr>\n
CAS Number<\/strong><\/td>\n37256-45-4<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nColorimetric<\/td>\n<\/tr>\n
Sample Types<\/strong><\/td>\nTissue extracts, Cell lysate<\/td>\n<\/tr>\n
Storage\/Stability<\/strong><\/td>\nShipped and store at 4\u00b0C for 6 months.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Name :<\/strong>
\nNitrate Reductase Microplate Assay Kit<\/p>\n

Reactivity :<\/strong>
\nAll<\/p>\n

Applications :<\/strong>
\nFunctional Assay<\/p>\n

Description :<\/strong>
\nDetection and Quantification of Nitrate Reductase Activity.<\/p>\n

Alternative Names :<\/strong>
\nEC 1.7.99.4; Nitrate reductase; Respiratory nitrate reductase<\/p>\n

CAS Number :<\/strong>
\n37256-45-4<\/p>\n

Detection Method :<\/strong>
\nColorimetric<\/p>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/a><\/p>\n

\u5373\u7528\u578bIHC Detection System kit\u00a0<\/a>\u00a0|\u00a0\u725b\u8840\u6e05\u767d\u86cb\u767d\u7c89 (BSA)\u00a0<\/a>\u00a0|\u00a0\u4f0a\u7d05 Y \u67d3\u8272\u7de9\u885d\u6db2<\/a>\u00a0|\u00a0<\/strong>PPOs \u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/p>\n

Bioworld\u53f0\u7063\u4ee3\u7406\u5546-BRAND\/AGENCY\/\u4ee3\u7406\u54c1\u724c\/ Bioworld\u53f0\u7063\u4ee3\u7406\u5546<\/p>\n

Bioworld \u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/h2>\n

Bioworld\u64c1\u6709\u5c08\u696d\u7d93\u9a57\u8c50\u5bcc\u7684\u79d1\u5b78\u548c\u6280\u8853\u5c08\u696d\u4eba\u54e1\uff0c\u88fd\u9020\u548c\u92b7\u552e\u6297\u9ad4\u3002 \u6211\u5011\u76ee\u524d\u7684\u6297\u9ad4\u8986\u84cb\u7bc4\u570d\u5ee3\u6cdb\u7684\u4fe1\u865f\u8f49\u5c0e\u548c\u7d30\u80de\u6311\u9078\uff0c\u4f8b\u5982\uff1a<\/p>\n

1\uff09\u67d3\u8272\u8cea\u548c\u9170\u5316\u4fe1\u865f<\/p>\n

2\uff09MAPK\u4fe1\u865f<\/p>\n

3\uff09\u795e\u7d93\u5143SAPK \/ JNK\u4fe1\u865f<\/p>\n

4\uff09\u7d30\u80de\u51cb\u4ea1\u548cCaspase\u4fe1\u865f<\/p>\n

5\uff09AKT\u4fe1\u865f<\/p>\n

6\uff09\u8f49\u9304\u548c\u7ffb\u8b6f\u63a7\u5236<\/p>\n

7\uff09PKC\u4fe1\u865f<\/p>\n

8\uff09\u7d30\u80de\u9031\u671f\u548cDNA\u640d\u50b7\u4fe1\u865f<\/p>\n

9\uff09\u7d30\u80de\u9aa8\u67b6\u4fe1\u865f<\/p>\n","protected":false},"excerpt":{"rendered":"

\u785d\u9178\u9e7d\u9084\u539f\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2 | Nitrate Reductase Microplate Assay Kit Bi […]<\/p>\n","protected":false},"author":3,"featured_media":31670,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[915],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31681"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31681"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31681\/revisions"}],"predecessor-version":[{"id":31688,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31681\/revisions\/31688"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31670"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31681"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31681"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31681"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}