{"id":29284,"date":"2021-09-09T17:40:19","date_gmt":"2021-09-09T09:40:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=29284"},"modified":"2022-07-19T13:41:45","modified_gmt":"2022-07-19T05:41:45","slug":"goat-anti-mouse-kappa-fitc%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f1050-02-%e8%a6%8f%e6%a0%bc1-0-ml-1-0-mgml","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=29284","title":{"rendered":"Goat Anti-Mouse Kappa-FITC\u6297\u9ad4 | \u8ca8\u865f1050-02 \u898f\u683c1.0 mL (1.0 mg\/mL)"},"content":{"rendered":"

Goat Anti-Mouse Kappa-FITC\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>1050-02\u00a0 <\/strong>\u898f\u683c<\/strong>1.0 mL \u00a0(1.0 mg\/mL)<\/a><\/span><\/p>\n

\"\u00e5\u0093\u0081\u00e7\u0089\u008c\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\"Goat<\/p>\n

BALB\/c mouse splenocytes were stained with Goat Anti-Mouse Kappa-FITC (SB Cat. No. 1050-02)<\/p>\n

\"\"<\/p>\n

Human pancreatic carcinoma cell line MIA PaCa-2 was stained with Mouse Anti-Cytokeratin 18-UNLB (SB Cat. No. 10085-01; right) followed by Goat Anti-Mouse Kappa-FITC (SB Cat. No. 1050-02) and DAPI.<\/p>\n

\"1050-02\"<\/a><\/p>\n

\u4e8c\u7d1a\u6297\u9ad4 (Secondary antibodies) \u61c9\u7528:<\/p>\n

\u4e8c\u6297(Secondary antibodies)\u662f\u91dd\u5c0d\u514d\u75ab\u7403\u86cb\u767d\uff08immunoglobulin , Ig\uff09\u5206\u5b50\u7684\u591a\u514b\u9686(polyclonal) \u548c\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibodies)\u3002\u5b83\u5011\u901a\u5e38\u8207\u6297\u539f\u7279\u7570\u6027\u4e00\u6297(specific primary antibody) \u4e00\u8d77\u4f7f\u7528\uff0c\u4e26\u4e14\u901a\u5e38\u88ab\u6a19\u5b9a\u9175\u7d20\u6216\u87a2\u5149\u7269\u8cea\u4ee5\u5e6b\u52a9\u6aa2\u6e2c\u3002\u4e8c\u6297\u53ef\u7528\u65bc\u591a\u7a2e\u61c9\u7528\uff0c\u4f8b\u5982ELISA\u3001\u514d\u75ab\u5370\u8de1(Immunoblotting)\u3001\u6d41\u5f0f\u7d30\u80de\u5100(Flow Cytometry)\u3001\u514d\u75ab\u7d44\u7e54\u67d3\u8272(Immunohistochemistry)\u3001\u514d\u75ab\u7d30\u80de\u5316\u5b78(Immuocytochemistry)\u3002\u4e8c\u7d1a\u6297\u9ad4\u901a\u5e38\u7528\u9176\u6216\u87a2\u5149\u67d3\u6599\u6a19\u8a18\uff0c\u9019\u4f7f\u5f97\u80fd\u5920\u901a\u904e\u6bd4\u8272\uff0c\u5316\u5b78\u767c\u5149\u548c\u87a2\u5149\u6280\u8853\u9032\u884c\u6aa2\u6e2c\u3002\u4f8b\u5982\uff0c\u9176\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08enzymes horseradish peroxidase , HRP\uff09\u548c\u9e7c\u6027\u78f7\u9178\u9176\uff08alkaline phosphatase , AP\uff09\u901a\u904e\u6bd4\u8272\u548c\u5316\u5b78\u767c\u5149\u5e73\u53f0\u5728ELISA\u548c\u86cb\u767d\u8cea\u5370\u8de1\u61c9\u7528\u4e2d\u662f\u6709\u50f9\u503c\u7684\u3002\u87a2\u5149\u86cb\u767d\u8af8\u5982\u87a2\u5149\u7d20\uff08fluorescein , FITC\uff09\uff0c\u85fb\u7d05\u86cb\u767d\uff08phycoerythrin , PE\uff09\u548c\u82b1\u9752\uff08cyanine , CY\uff09\u7684\u87a2\u5149\u67d3\u6599\u5728\u87a2\u5149\u7684\u61c9\u7528\u4e2d\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6d41\u5f0f\u7d30\u80de\u8853\uff0c\u514d\u75ab\u87a2\u5149\u986f\u5fae\u8853\u548c\u591a\u91cd\u6e2c\u5b9a\u3002\u4e8c\u6297\u5141\u8a31\u6a19\u8a18\u548c\u6aa2\u6e2c\u7684\u9748\u6d3b\u6027\u4ee5\u53ca\u901a\u904e\u4fe1\u865f\u653e\u5927\uff0c\u53ef\u589e\u52a0\u7684\u9748\u654f\u5ea6\u3002<\/p>\n

\"\u00e7\u0094\u00a2\u00e5\u0093\u0081\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Goat Anti-Mouse Kappa-FITC\u6297\u9ad4\uff0c\u5177FITC\u6a19\u8a8c\u3002\u91dd\u5c0dmouse \u03ba light chains\u8f15\u93c8\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baPooled antisera from goats hyperimmunized with mouse \u03ba light chains\u3002Isotype\u70baGoat IgG\u3002Isotype Control Goat IgG-FITC<\/a>\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/strong><\/td>\nReacts with mouse \u03ba light chains<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from goats hyperimmunized with mouse \u03ba light chains<\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nMouse \u03bb light chains; may react with \u03ba light chains from other species<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography on mouse \u03ba light chains covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nGoat IgG-FITC<\/a><\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nFITC (Fluorescein)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing < 0.1% sodium azide<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1.0 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

ELISA\u00a01-11<\/sup>
\nFLISA
\nFlow Cytometry\u00a03,8,12,15,16,26<\/sup><\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

ELISpot\u00a02,7,12-14<\/sup>
\nImmunohistochemistry-Frozen Sections\u00a017<\/sup>
\nImmunohistochemistry-Paraffin Sections\u00a018,19<\/sup>
\nImmunocytochemistry\u00a05,20-22<\/sup>
\nWestern Blot\u00a01,4,6,8,9,15,26<\/sup>
\nImmunoprecipitation\u00a023,24<\/sup>
\nStimulation\u00a025,26<\/sup>
\nSurface Plasmon Resonance\u00a027<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/p>\n

\"SouthernBiotech0100-01\"<\/a><\/p>\n

\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"+27 References<\/p>\n

    \n
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  2. Sangster MY, Topham DJ, D’Costa S, Cardin RD, Marion TN, Myers LK, et al. Analysis of the virus-specific and nonspecific B cell response to a persistent B-lymphotropic gammaherpesvirus. J Immunol. 2000;164:1820-8. (ELISA, ELISPOT)<\/li>\n
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  8. Xia Y, Pawar RD, Nakouzi AS, Herlitz L, Broder A, Liu K, et al. The constant region contributes to the antigenic specificity and renal pathogenicity of murine anti-DNA antibodies. J Autoimmun. 2012;39:398-411. (ELISA, FC, WB)<\/li>\n
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  11. Sang A, Niu H, Cullen J, Choi SC, Zheng YY, Wang H, et al. Activation of rheumatoid factor-specific B cells is antigen dependent and occurs preferentially outside of germinal centers in the lupus-prone NZM2410 mouse model. J Immunol. 2014;193:1609-21. (ELISA)<\/li>\n
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  13. Sharma S, Campbell AM, Chan J, Schattgen SA, Orlowski GM, Nayar R, et al. Suppression of systemic autoimmunity by the innate immune adaptor STING. Proc Natl Acad Sci USA. 2015;112:E710-7. (ELISPOT)<\/li>\n
  14. Ratliff M, Alter S, McAvoy K, Frasca D, Wright JA, Zinkel SS, et al. In aged mice, low surrogate light chain promotes pro-B-cell apoptotic resistance, compromises the PreBCR checkpoint, and favors generation of autoreactive, phosphorylcholine-specific B cells. Aging Cell. 2015;14:382-90. (ELISPOT)<\/li>\n
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  27. Swearingen CA, Carpenter JW, Siegel R, Brittain IJ, Dotzlaf J, Durham TB, et al. Development of a novel clinical biomarker assay to detect and quantify aggrecanase-generated aggrecan fragments in human synovial fluid, serum and urine. Osteoarthritis Cartilage. 2010;18:1150-8. (Surface Plasmon Resonance)<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    Goat Anti-Mouse Kappa-FITC\u6297\u9ad4 | \u8ca8\u865f1050-02\u00a0 \u898f\u683c1.0 mL \u00a0(1. […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[198],"tags":[1017],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29284"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=29284"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29284\/revisions"}],"predecessor-version":[{"id":29288,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29284\/revisions\/29288"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=29284"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=29284"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=29284"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}