{"id":28777,"date":"2021-08-25T17:17:45","date_gmt":"2021-08-25T09:17:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28777"},"modified":"2023-03-13T11:56:30","modified_gmt":"2023-03-13T03:56:30","slug":"mini%e8%b3%aa%e9%ab%94dna%e8%90%83%e5%8f%96%e5%a5%97%e7%b5%84-%e8%a9%a6%e5%8a%91%e7%9b%92-plasmid-miniprep-kit-%e8%b2%a8%e8%99%9fpdp01-0100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=28777","title":{"rendered":"mini\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44 \u8a66\u5291\u76d2| Plasmid miniPREP Kit \u8ca8\u865fPDP01-0100"},"content":{"rendered":"<p><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=28777\">mini\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44 \u8a66\u5291\u76d2<strong>| <\/strong><u>Plasmid miniPREP\u00a0Kit<\/u>\u00a0 <strong>\u8ca8\u865f<\/strong>PDP01-0100<\/a>\u00a0100\u500b\u53cd\u61c9<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E7%90%86%E4%BB%8B%E7%B4%B9-300x114.png\" alt=\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" width=\"93\" height=\"35\" \/><br \/>\nPureDireX\u5c0f\u91cf\u8cea\u9ad4DNA (plasmid DNA, PDP01-0100) <u>\u8a66\u5291\u76d2<\/u>\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u4e14\u6210\u672c\u6709\u6548\u5730\u7528\u65bc\u5f9e\u57f9\u990a\u7684\u9769\u862d\u6c0f\u967d\u6027(Gram positive)\u548c\u9769\u862d\u6c0f\u9670\u6027\u7d30\u83cc\u7d30\u80de(Gram negative bacterial cells)\u5206\u96e2\u8cea\u9ad4DNA (plasmid DNA)\u7684\u65b9\u6cd5\u3002\u8a72\u8a2d\u8a08\u57fa\u65bc\u7d30\u83cc\u7d30\u80de\u7684\u9e7c\u88c2\u89e3(alkaline lysis)\uff0c\u7136\u5f8c\u5728\u5b58\u5728\u9ad8\u6fc3\u5ea6\u9e7d\u7684\u60c5\u6cc1\u4e0b\u5c07 DNA \u7d50\u5408\u5230\u96e2\u5fc3\u67f1(spin column)\u7684\u73bb\u7483\u7e96\u7dad\u57fa\u8cea(glass fiber matrix)\u4e0a\u3002\u4f7f\u7528\u8a72\u8a66\u5291\u76d2\u7d14\u5316\u7684\u8cea\u9ad4DNA (plasmid DNA)\u9069\u7528\u65bc\u5404\u7a2e\u5e38\u898f\u61c9\u7528\uff0c\u5305\u62ec\u9650\u5236\u6027\u5167\u5207\u9176\u6d88\u5316(restriction enzyme digestion)\u3001\u6e2c\u5e8f(sequencing)\u3001\u6587\u5eab\u7be9\u9078(library screening)\u3001<em>\u9ad4\u5916<\/em>\u8f49\u8b6f\u00a0 (<em>in vitro<\/em>\u00a0translation)\u3001\u7d30\u80de\u8f49\u67d3(transfection)\u3001\u9023\u63a5(ligation)\u548c\u8f49\u5316(transformation)\u3002\u6574\u500b\u904e\u7a0b\u53ef\u4ee5\u572815-20\u5206\u9418\u5167\u5b8c\u6210\u3002<br \/>\n<img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/03\/mini%E8%B3%AA%E9%AB%94DNA%E8%90%83%E5%8F%96%E5%A5%97%E7%B5%84-%E8%A9%A6%E5%8A%91%E7%9B%92-Plasmid-miniPREP-Kit-%E8%B2%A8%E8%99%9FPDP01-0100.jpg\" alt=\"\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/03\/%E7%94%A2%E5%93%81%E7%89%B9%E9%BB%9E_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7522\u54c1\u7279\u9ede_\u5de5\u4f5c\u5340\u57df 1\" width=\"145\" height=\"59\" \/><\/p>\n<p><strong>\u6700\u5feb\u7684\u7a0b\u5e8f\uff1a<\/strong>\u00a0\u5728 15-20 \u5206\u9418\u5167\u5b8c\u6210<br \/>\n<strong>\u6a23\u54c1\uff1a<\/strong>\u00a0\u6700\u591a4 ml\u7d30\u83cc\u7d30\u80de<br \/>\n<strong>\u7522\u91cf\uff1a<\/strong>\u00a0\u6700\u591a30\u03bcg\u8cea\u7c92<\/p>\n<p>Note: PureDireX \/ Purification Kits \/ Extraction Kit \/ Plasmid DNA<br \/>\nCategories:\u00a0<a href=\"http:\/\/bio-helix.com\/products?category_id=27\">Plasmid DNA Purification System<\/a><\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 12pt; color: #800000;\"><strong>Plasmid miniPREP Kit <\/strong><\/span><\/p>\n<p><span style=\"color: #3366ff;\">Protocol Step 1<\/span> Bacterial Cells Harvesting<\/p>\n<p>1. Transfer 1.5 ml bacterial culture to a microcentrifuge tube.<\/p>\n<p>2. Centrifuge at 14,000 x g for 1 minute and discard the supernatant.<\/p>\n<p><span style=\"color: #3366ff;\">Step 2 Resuspend <\/span><\/p>\n<p>1. Resuspend pelleted bacterial cells in 200 \u03bcl of the Buffer S1 (RNase A added).<\/p>\n<p>Step 3 Lysis<\/p>\n<p>1.Add 200 \u03bcl of the Buffer S2 and mix thoroughly by inverting the tube 10 times (do not vortex) and then stand at the room temperature for 2 minutes or until the lysate is homologous.<\/p>\n<p>Step 4 Neutralization 1.Add 300 \u03bcl of the Buffer S3 and mix immediately and thoroughly by inverting the tube 10 times (Do not vortex). 2. Centrifuge at 14,000 x g for 3 minutes.<\/p>\n<p>Step 5 Binding 1.Place a PM column in a Collection Tube. Apply the supernatant (from step 4) to the PM column by decanting or pipetting. 2. Centrifuge at 14,000 x g for 30 seconds, then discard the flow-through, and place the PM column back into the same collection tube.<\/p>\n<p>Step 6 Wash 1.Add 400 \u03bcl of the Buffer W1 into the PM column. 2. Centrifuge at 14,000 x g for 30 seconds. 3. Discard the flow-through and place the PM column back into the same collection tube. 4.Add 600 \u03bcl of the Buffer W2 (Ethanol added) into the PM column. 5. Centrifuge at 14,000 x g for 30 seconds. 6. Discard the flow-through and place the PM column back into the same collection tube. 7. Centrifuge at 14,000 x g again for 2 minutes to remove the residual Buffer W2. \u00a0Step 7 Elution 1. To elute DNA, place the PM column in a clean 1.5 ml microcentrifuge tube. 2.Add 50~200 \u03bcl of the Buffer E or H2O (pH between 7.0 and 8.5) to the center of each PM column, let it stand for 2 minutes, and centrifuge at 14,000 x g for 2 minutes. \uff0aCheck the buffers before use for salt precipitation. Redissolve any precipitate by warming to 37\u00b0C.<\/p>\n<p><span style=\"color: #008000; font-size: 12pt;\"><a style=\"color: #008000;\" href=\"http:\/\/biopioneer.com.tw\/?p=9187\"><strong>\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a><strong>\u00a0<span style=\"color: #808080;\">|\u00a0<\/span>\u00a0<\/strong><a style=\"color: #008000;\" href=\"http:\/\/biopioneer.com.tw\/?p=16198\"><strong>Finnpipette F3\u00a0<\/strong><strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a><strong>\u00a0<span style=\"color: #808080;\">|<\/span>\u00a0\u00a0<\/strong><a style=\"color: #008000;\" href=\"http:\/\/biopioneer.com.tw\/?p=17586\"><strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a><strong>\u00a0<span style=\"color: #808080;\">|\u00a0<\/span>\u00a0<\/strong><a style=\"color: #008000;\" href=\"http:\/\/biopioneer.com.tw\/?p=19075\"><strong>Genereach<\/strong><strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a><strong>\u00a0<span style=\"color: #808080;\">\u00a0|\u00a0<\/span>\u00a0\u00a0<\/strong><a style=\"color: #008000;\" href=\"http:\/\/biopioneer.com.tw\/?p=1120\"><strong>ELISA<\/strong><strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2023\/02\/PureDirex.png\" alt=\"\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/01\/%E7%B6%B2%E7%AB%99%E8%A6%8F%E6%A0%BC%E5%B0%8F%E5%9C%96-%E5%93%81%E7%89%8C%E4%BB%8B%E7%B4%B9_%E5%B7%A5%E4%BD%9C%E5%8D%80%E5%9F%9F-1-300x120.jpg\" alt=\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716 \u54c1\u724c\u4ecb\u7d39_\u5de5\u4f5c\u5340\u57df 1\" width=\"146\" height=\"60\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2023\/02\/BIO-HIDEX-300x47.png\" alt=\"BIO-HIDEX\" width=\"214\" height=\"30\" \/><\/p>\n<p>bio-helix.com<\/p>\n<p>Bio-Helix\u6210\u7acb\u65bc2007\u5e74\uff0c\u5c08\u6ce8\u65bc\u70ba\u5168\u7403\u751f\u547d\u79d1\u5b78\u7814\u7a76\u4eba\u54e1\u63d0\u4f9b\u6700\u597d\u7684\u751f\u7269\u8a66\u5291\u3002\u5305\u62ec DNA \u6a19\u8a18\u7269\u3001\u86cb\u767d\u8cea\u6a19\u8a18\u7269\u3001\u9810\u88fd\u86cb\u767d\u8cea\u51dd\u81a0\u3001\u6838\u9178\u7d14\u5316\u3001PCR\u8a66\u5291\u7b49\u3002\u8207\u4e16\u754c\u5404\u5730\u5be6\u9a57\u5ba4\u7684\u79d1\u5b78\u5bb6\u5011\u4e00\u8d77\u52aa\u529b\uff0c\u4e0d\u65b7\u63d0\u9ad8\u8cea\u91cf\uff0c\u958b\u59cb\u8d0f\u5f97\u5ba2\u6236\u7684\u4fe1\u4efb\u548c\u8a55\u50f9\u3002\u54c1\u724c\u904d\u5e03\u56db\u5341\u591a\u500b\u570b\u5bb6\uff0c\u5df2\u6210\u70ba\u751f\u547d\u79d1\u5b78\u5be6\u9a57\u5ba4\u503c\u5f97\u4fe1\u8cf4\u548c\u6b61\u8fce\u7684\u8a66\u5291\u54c1\u724c\u3002Bio-Helix\u500b\u9858\u666f\uff0c\u5c31\u662f\u6709\u4e00\u5929\u6211\u5011\u7684\u58fd\u547d\u53ef\u4ee5\u5ef6\u9577\uff1b\u6240\u6709\u75be\u75c5\u7684\u65e9\u671f\u767c\u73fe\u548c\u96a8\u5f8c\u7684\u9069\u7576\u6cbb\u7642\u90fd\u53ef\u4ee5\u63d0\u9ad8\u751f\u6d3b\u8cea\u91cf\u3002\u4e2d\u570b\u53e4\u4eba\u8a8d\u70ba\uff0c\u826f\u91ab\u6cbb\u672a\u75c5\uff0c\u6cbb\u672a\u75c5\u3002\u5728 Bio-Helix \u4e2d\uff0c\u5275\u5efa\u4e86\u4e00\u500b\u65b0\u54c1\u724c LifeDirex \u4f86\u5be6\u73fe\u9019\u4e00\u9858\u666f\u3002 LifeDirex \u5c07\u5c08\u6ce8\u65bc\u70ba\u8a3a\u65b7\u5de5\u5177\u63d0\u4f9b\u6700\u597d\u548c\u6700\u5177\u5275\u65b0\u6027\u7684\u8a66\u5291\uff0c\u4ee5\u4fc3\u9032\u76e1\u53ef\u80fd\u65e9\u5730\u767c\u73fe\u75be\u75c5\u3002Bio-Helix\u4f7f\u547d\u662f\u63d0\u4f9b\u6700\u512a\u8cea\u7684\u7522\u54c1\u4e26\u958b\u767c\u65b0\u6280\u8853\uff0c\u5e6b\u52a9\u7814\u7a76\u4eba\u54e1\u627e\u5230\u5c0b\u6c42\u79d1\u5b78\u6210\u5c31\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6b64\u5916\uff0cBio-Helix\u8a8d\u70ba\u73fe\u5728\u662f\u9032\u5165\u9ad4\u5916\u8a3a\u65b7\u8a2d\u5099\uff08IVDD\uff09\u5e02\u5834\u7684\u6700\u4f73\u6642\u6a5f\u3002\u6211\u5011\u7684\u76ee\u6a19\u662f\u6210\u70ba\u7814\u7a76\u5be6\u9a57\u5ba4\u548cIVDD\u7684\u6700\u4f73\u89e3\u6c7a\u65b9\u6848\u63d0\u4f9b\u5546\u3002<\/p>\n<p>\u5728\u6539\u5584\u751f\u6d3b\u8cea\u91cf\u548c\u5be6\u73fe\u79d1\u5b78\u5353\u8d8a\u7684\u9858\u671b\u7684\u9a45\u4f7f\u4e0b\uff0cBio-Helix\u4ee5\u6700\u9ad8\u7684\u8aa0\u4fe1\u6a19\u6e96\u904b\u71df\u3002\u9084\u5c07\u9019\u7a2e\u7cbe\u795e\u5ef6\u4f38\u5230\u6211\u5011\u7684\u670d\u52d9\u548c\u5168\u7403\u50f9\u503c\u4e2d\uff0c\u4ee5\u63d0\u4f9b\u7d66\u4e16\u754c\u5404\u5730\u7684\u5ba2\u6236\u3002\u591a\u6a23\u5316\u7684\u54e1\u5de5\u968a\u4f0d\u548c\u5354\u4f5c\u7db2\u7d61\u5f62\u6210\u4e86\u516c\u53f8\u6587\u5316\u7684\u5b8c\u7f8e\u7d50\u5408\u3002 Bio-Helix \u4e0d\u50c5\u662f\u4e00\u5bb6\u6a5f\u6703\u5747\u7b49\u7684\u516c\u53f8\uff0c\u800c\u4e14\u9084\u52aa\u529b\u63d0\u4f9b\u53cb\u597d\u548c\u6210\u9577\u7684\u74b0\u5883\u3002<\/p>\n<table width=\"994\">\n<tbody>\n<tr>\n<td width=\"168\">CAT#<\/td>\n<td width=\"647\">NAME<\/td>\n<td width=\"179\">Size<\/td>\n<\/tr>\n<tr>\n<td>BP001CU<\/td>\n<td width=\"647\">BluPAD, LED Transilluminator, with mini Darkroom<br \/>\n(CE\/ETL, Patent No: M543442)<\/td>\n<td>Set<\/td>\n<\/tr>\n<tr>\n<td>PMI09-0500<\/td>\n<td width=\"647\">IRIS9 Plus Prestained Protein Ladder<br \/>\n(9 pre-stained bands, 15-170 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>PMI11-0500<\/td>\n<td width=\"647\">IRIS11 Prestained Protein Ladder<br \/>\n(11 pre-stained bands, 3-260 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>PMB01-0500<\/td>\n<td width=\"647\">Blu10 Plus Prestained Protein Ladder<br \/>\n(10 pre-stained bands, 6.5-270 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PMB10-0500<\/td>\n<td width=\"647\">Blu10 Prestained Protein Ladder<br \/>\n(10 pre-stained bands, 11-180 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PMB11-0500<\/td>\n<td width=\"647\">Blu11 Prestained Protein Ladder<br \/>\n(11 pre-stained bands, 10-180 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PMB12-0500<\/td>\n<td width=\"647\">Blu12 Prestained Protein Ladder<br \/>\n(12 pre-stained bands, 11-245 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PMB13-0500<\/td>\n<td width=\"647\">Blu13 Prestained Protein Ladder<br \/>\n(13 pre-stained bands, 5-245 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>PMU12-0500<\/td>\n<td width=\"647\">UNveil Unstained Protein Ladder, Broad Range<br \/>\n(12 unstained bands, 10-200 kDa)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>CCH321-B100ML<\/td>\n<td width=\"647\">UltraScence Pico Plus Western Substrate<br \/>\n(Patent No: US10,711,185)<\/td>\n<td width=\"179\">100 ml<br \/>\n(50ml+50ml)<\/td>\n<\/tr>\n<tr>\n<td>CCH345-B100ML<\/td>\n<td width=\"647\">UltraScence Pico Ultra Western Substrate<br \/>\n(Patent No: US10,711,185)<\/td>\n<td width=\"179\">100 ml<br \/>\n(50ml+50ml)<\/td>\n<\/tr>\n<tr>\n<td>CCH365-B100ML<\/td>\n<td width=\"647\">UltraScence Femto Western Substrate<br \/>\n(Patent No: US10,711,185)<\/td>\n<td width=\"179\">100 ml<br \/>\n(50ml+50ml)<\/td>\n<\/tr>\n<tr>\n<td>CCH375-B100ML<\/td>\n<td width=\"647\">UltraScence Femto Plus Western Substrate<br \/>\n(Patent No: US10,711,185)<\/td>\n<td width=\"179\">100 ml<br \/>\n(50ml+50ml)<\/td>\n<\/tr>\n<tr>\n<td>PS002-B500ML<\/td>\n<td>COOMASSIEnano Protein Staining Solution, 500ml<\/td>\n<td>500 ml<\/td>\n<\/tr>\n<tr>\n<td>PS003-B500ML<\/td>\n<td>Ponceau S Protein Staining Solution<\/td>\n<td>500 ml<\/td>\n<\/tr>\n<tr>\n<td>BS001-B500ML<\/td>\n<td width=\"647\">OneStep Blocker<br \/>\nWestern Blocking Solution and Signal Enhancer<\/td>\n<td>500 ml<\/td>\n<\/tr>\n<tr>\n<td>TM001-B200ML<\/td>\n<td>TMB ELISA Substrate<\/td>\n<td width=\"179\">100 ml<br \/>\n(50ml+50ml)<\/td>\n<\/tr>\n<tr>\n<td>DM001-R500<\/td>\n<td width=\"647\">BH 100bp DNA Ladder RTU<br \/>\n(11 bands, 100-1,500bps)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>DM003-R500<\/td>\n<td width=\"647\">BH 100bp DNA Ladder H3 RTU<br \/>\n(12 bands, 100-3,000bps)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>DM012-R500<\/td>\n<td width=\"647\">BH 50bp DNA Ladder RTU<br \/>\n(17 bands, 50-1,500bps)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>DM015-R500<\/td>\n<td width=\"647\">BH 1Kb Plus DNA Ladder RTU<br \/>\n(13 bands, 100-10,000bps)<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">DMF12-0100<\/td>\n<td width=\"647\">OmniMARK 100 DNA Ladder RTU<br \/>\n(12 bands, 100-3,000bps, fluorescent dye)<\/td>\n<td>600 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">DMF13-0100<\/td>\n<td width=\"647\">OmniMARK 1K DNA Ladder RTU<br \/>\n(13 bands, 100-10,000bps, fluorescent dye)<\/td>\n<td>600 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>LD011-1000<\/td>\n<td>Prime Juice Preloading Fluorescent Stain<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>LD003-0500<\/td>\n<td>Novel Green Plus (20,000X) DNA Staining Reagent<\/td>\n<td>500 \u03bcl<\/td>\n<\/tr>\n<tr>\n<td>AGT002-0500<\/td>\n<td>Agarose Tablets, 110 pcs<\/td>\n<td>0.5g x 110<\/td>\n<\/tr>\n<tr>\n<td>MB755-0100<\/td>\n<td>Agarose Powder 100g -Molecular Biology Grade<\/td>\n<td>100 g<\/td>\n<\/tr>\n<tr>\n<td>MB755-0500<\/td>\n<td>Agarose Powder 500g -Molecular Biology Grade<\/td>\n<td>500 g<\/td>\n<\/tr>\n<tr>\n<td>MB101-0500<\/td>\n<td>Taq DNA Polymerase<\/td>\n<td>500U\/100\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>DP001-0100<\/td>\n<td>nanoTaq\u2122 Hot-Start DNA Polymerase<\/td>\n<td width=\"179\">100 rxns<br \/>\n500U\/100\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>DN001-0250<\/td>\n<td>100 mM dNTP Set<\/td>\n<td>4 x 250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>DN0010<\/td>\n<td>10 mM dNTP Mix<\/td>\n<td>1 ml<\/td>\n<\/tr>\n<tr>\n<td>MB200-P100<\/td>\n<td>2X PCR SuperMix<\/td>\n<td width=\"179\">100 rxns<br \/>\n2 x 1.25mL<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">MBA01-0100<\/td>\n<td width=\"647\">OmniPCR Supermix w Fluorescent dye<\/td>\n<td width=\"179\">100 rxns<br \/>\n(2.5ml)<\/td>\n<\/tr>\n<tr>\n<td>RK001-0050<\/td>\n<td>RScript\u2122 cDNA Synthesis Kit<\/td>\n<td>50 rxns<\/td>\n<\/tr>\n<tr>\n<td>RT001-0050<\/td>\n<td>RScript\u2122 Reverse Transcriptase 10,000U<\/td>\n<td width=\"179\">50 rxns<br \/>\n10,000U\/50\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>RT001-0250<\/td>\n<td>RScript\u2122 Reverse Transcriptase 50,000U<\/td>\n<td width=\"179\">250 rxns<br \/>\n50,000U\/250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>RI001-0125<\/td>\n<td>RIBOAssure\u2122 RNase Inhibitor 5,000U<\/td>\n<td width=\"179\">125 rxns<br \/>\n5,000U\/125\u03bcl<\/td>\n<\/tr>\n<tr>\n<td>QPR01-0100<\/td>\n<td>PanProbes\u2122 One-Step RT-qPCR Kit<\/td>\n<td width=\"179\">100 reactions<br \/>\n(20 \u03bcl vol)<\/td>\n<\/tr>\n<tr>\n<td>QPD01-0100<\/td>\n<td>PanProbes\u2122 Universal qPCR MasterMix<\/td>\n<td width=\"179\">100 reactions<br \/>\n(20 \u03bcl vol)<\/td>\n<\/tr>\n<tr>\n<td>QSR01-0100<\/td>\n<td>PanGreen\u2122 One-Step RT-qPCR Kit<\/td>\n<td width=\"179\">100 reactions<br \/>\n(20 \u03bcl vol)<\/td>\n<\/tr>\n<tr>\n<td>QSD01-0100<\/td>\n<td>PanGreen\u2122 Universal SYBR\u00ae Green Mastermix<\/td>\n<td width=\"179\">100 reactions<br \/>\n(20 \u03bcl vol)<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDP01-0100<\/td>\n<td width=\"647\">Plasmid miniPREP Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDP02-0020<\/td>\n<td width=\"647\">Plasmid midiPREP Kit<\/td>\n<td width=\"179\">20 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDP03-0010<\/td>\n<td width=\"647\">Plasmid maxiPREP Kit<\/td>\n<td width=\"179\">10 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC01-0100<\/td>\n<td width=\"647\">PCR Clean-Up &amp; Gel Extraction Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC02-0100<\/td>\n<td width=\"647\">Genomic DNA isolation dual kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC05-0100<\/td>\n<td width=\"647\">Dual Genomic DNA Isolation Kit (Plant)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC06-0100<\/td>\n<td width=\"647\">Dual Genomic DNA Isolation Kit(Tissue)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC09-0100<\/td>\n<td width=\"647\">Genomic DNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC10-0100<\/td>\n<td width=\"647\">Genomic DNA Isolation Kit (Plant)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC11-0100<\/td>\n<td width=\"647\">Genomic DNA Isolation Kit (Tissue)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC12-0100<\/td>\n<td width=\"647\">Genomic DNA Isolation Kit (Paraffin-embedded tissue)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDM01-0100<\/td>\n<td width=\"647\">Mbead Buffy Coat Genomic DNA Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDM02-0100<\/td>\n<td width=\"647\">Mbead Tissue Genomic DNA Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDM03-0100<\/td>\n<td width=\"647\">Mbead Bacteria Genomic DNA Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDM05-0100<\/td>\n<td width=\"647\">Mbead Plant Genomic DNA Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDM06-0100<\/td>\n<td width=\"647\">Mbead Blood\/Cell Genomic DNA Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDR01-0100<\/td>\n<td width=\"647\">Genomic DNA Isolation Reagent<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDR02-0100<\/td>\n<td width=\"647\">Plant Genomic DNA Isolation Reagent<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDR05-0100<\/td>\n<td width=\"647\">Genomic DNA Isolation Reagent Kit(Blood\/Cultured Cell\/Tissue)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC04-0100<\/td>\n<td width=\"647\">Total RNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC07-0100<\/td>\n<td width=\"647\">Total RNA Isolation Kit (Plant)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC08-0100<\/td>\n<td width=\"647\">Total RNA Isolation Kit(Tissue)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDR03-0100<\/td>\n<td width=\"647\">Total RNA Isolation Reagent<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDR04-0100<\/td>\n<td width=\"647\">Plant Total RNA Isolation Reagent<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDC03-0100<\/td>\n<td width=\"647\">Virus Nucleic Acid Isolation Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">PDM04-0100<\/td>\n<td width=\"647\">Mbead Virus Genomic Nucleic Acid Kit<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">QP019M-0050<\/td>\n<td width=\"647\">COVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)<\/td>\n<td width=\"179\">50 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">QP019T-0100<\/td>\n<td width=\"647\">COVID-19 RT-qPCR Detection Kit Plus (TFDA)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<tr>\n<td width=\"168\">QP019-0100<\/td>\n<td width=\"647\">SARS-CoV-2\/COVID-19 RT-qPCR Detection Kit (RUO)<\/td>\n<td width=\"179\">100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>mini\u8cea\u9ad4DNA\u8403\u53d6\u5957\u7d44 \u8a66\u5291\u76d2| Plasmid miniPREP\u00a0Kit\u00a0 \u8ca8\u865fPDP01-0100\u00a01 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":34717,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5372,3],"tags":[1059],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28777"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28777"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28777\/revisions"}],"predecessor-version":[{"id":40001,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28777\/revisions\/40001"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34717"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28777"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28777"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28777"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}