{"id":28697,"date":"2021-08-20T21:20:27","date_gmt":"2021-08-20T13:20:27","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28697"},"modified":"2024-09-10T14:09:42","modified_gmt":"2024-09-10T06:09:42","slug":"%e9%b9%bc%e6%80%a7%e7%a3%b7%e9%85%b8%e9%85%b6-alkaline-phosphatase-%e6%9f%93%e8%89%b2%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk2035","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=28697","title":{"rendered":"\u9e7c\u6027\u78f7\u9178\u9176 (Alkaline Phosphatase ) \u67d3\u8272\u8a66\u5291\u76d2 | \u8ca8\u865fK2035 (ABCAM ab284936)"},"content":{"rendered":"

\u9e7c\u6027\u78f7\u9178\u9176 (Alkaline Phosphatase ) \u67d3\u8272\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865f<\/strong>(ABCAM ab284936) K2035<\/a><\/span>\"\u00e7\u0094\u00a2\u00e5\u0093\u0081\u00e5\u009e\u008b\u00e9\u008c\u0084\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

Alkaline Phosphatase Staining Kit<\/em><\/strong><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\"\u00e7\u0094\u00a2\u00e5\u0093\u0081\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/p>\n

\u9e7c\u6027\u78f7\u9178\u9176 (Alkaline Phosphatase ,AP) \u67d3\u8272\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u7c21\u4fbf\u7684\u65b9\u6cd5\u4f86\u5c0d\u591a\u80fd\u5e79\u7d30\u80de\u4e2d\u7684\u9e7c\u6027\u78f7\u9178\u9176(Alkaline Phosphatase)\u9032\u884c\u67d3\u8272\u3002 \u5b83\u57fa\u65bc\u9e7c\u6027\u78f7\u9178\u9176(Alkaline Phosphatase)\u6c34\u89e3\u5e95\u7269 5-\u6eb4-4-\u6c2f-3-\u5432\u54da\u57fa\u78f7\u9178\u916f (5-Bromo-4-chloro-3-indolyl phosphate , BCIP) \u4e0a\u7684\u78f7\u9178\u57fa\u5718\u4ee5\u5f62\u6210\u85cd\u8272\u4e2d\u9593\u9ad4\u7684\u80fd\u529b\u3002 (Nitro Blue Tetrazolium , NBT) \u7136\u5f8c\u5c07\u4e2d\u9593\u9ad4\u6c27\u5316\u5f62\u6210\u4e0d\u6eb6\u7684\u6df1\u85cd\u8272\u4e8c\u805a\u9ad4\u3002\u7531\u65bc\u5e79\u7d30\u80de\/\u591a\u80fd\u5e79\u7d30\u80de\u7684 AP \u6d3b\u6027\u589e\u52a0\uff0c\u56e0\u6b64 AP \u67d3\u8272\u8a66\u5291\u76d2\u53ef\u7528\u65bc\u9451\u5b9a\u548c\u7be9\u9078\u9019\u4e9b\u7d30\u80de\u4e2d\u7684\u9e7c\u6027\u78f7\u9178\u9176\u6d3b\u6027(Alkaline Phosphatase activity)\u3002 \u8a72\u67d3\u8272\u65b9\u6cd5\u7c21\u55ae\u3001\u5feb\u901f\u3001\u7d93\u6fdf\u3001\u53ef\u9760\u3002 \u6574\u500b\u904e\u7a0b\u53ef\u4ee5\u5728\u540c\u4e00\u500b\u5fae\u91cf\u6ef4\u5b9a\u677f\u4e0a\u9032\u884c\uff0c\u4e0d\u9700\u8981\u4efb\u4f55\u984d\u5916\u7684\u6b65\u9a5f\uff0c\u5982\u56fa\u5b9a\u6216\u6536\u7a6b\u7d30\u80de\u3002 \u8a72\u6e2c\u5b9a\u9084\u53ef\u7528\u4f5c\u6e2c\u8a66\u7d30\u80de\u591a\u80fd\u6027\u548c\u76e3\u6e2c\u591a\u80fd\/\u5e79\u7d30\u80de\u7684\u6210\u9aa8\u5206\u5316\u7684\u521d\u59cb\u6b65\u9a5f\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK2035-50 (ABCAM ab284936)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n50 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nStaining followed by microscopy<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Screening for pluripotent cells induced by various factors.
\n\u2022 Detection of alkaline phosphate activity in stem cells\/pluripotent stem cells, HeLa, HepG2, HEK293 cells, osteoblasts and osteocytes.
\n\u2022 AP Staining in stem cells\/pluripotent stem cells or stem cells undergoing osteogenesis.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 To identify and screen Alkaline Phosphatase activity in cells
\n\u2022 Rapid
\n\u2022 Cost-effective
\n\u2022 Reliable
\n\u2022 Simple<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Wash Buffer
\n\u2022 AP Staining Reagent<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00baC<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u9e7c\u6027\u78f7\u9178\u9176 (Alkaline Phosphatase ) \u67d3\u8272\u8a66\u5291\u76d2 | \u00a0\u8ca8\u865f(ABCAM ab28493 […]<\/p>\n","protected":false},"author":3,"featured_media":28698,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1062],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28697"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28697"}],"version-history":[{"count":5,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28697\/revisions"}],"predecessor-version":[{"id":50281,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28697\/revisions\/50281"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28698"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28697"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28697"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28697"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}