{"id":28494,"date":"2021-08-17T14:21:04","date_gmt":"2021-08-17T06:21:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28494"},"modified":"2022-07-19T13:59:02","modified_gmt":"2022-07-19T05:59:02","slug":"%e9%80%8f%e6%98%8e%e8%b3%aa%e9%85%b8%e9%85%b6%e6%b4%bb%e6%80%a7-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fe4909-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=28494","title":{"rendered":"\u900f\u660e\u8cea\u9178\u9176\u6d3b\u6027 ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fE4909-100"},"content":{"rendered":"

\"\"\u900f\u660e\u8cea\u9178\u9176\u6d3b\u6027 ELISA \u8a66\u5291\u76d2 | \u00a0\u8ca8\u865f<\/strong>E4909-100<\/a><\/span><\/p>\n

Hyaluronidase Activity ELISA Kit<\/em><\/strong><\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u900f\u660e\u8cea\u9178\u9176(Hyaluronidases)\uff0c\u4e5f\u7a31\u70ba\u900f\u660e\u8cea\u9178\u8461\u8404\u7cd6\u82f7\u9176 (hyaluronoglucosidases ,HYAL)\uff0c\u662f\u901a\u904e\u88c2\u89e3\u900f\u660e\u8cea\u9178 (hyaluronic acid , HA) \u7684\u7cd6\u82f7\u9375(glycosidic bonds)\u4f86\u50ac\u5316\u5176\u964d\u89e3\u7684\u9176\u5bb6\u65cf\u3002 HA \u662f\u4e00\u7a2e\u975e\u786b\u9178\u5316(nonsulfated glycosaminoglycan)\u7684\u7cd6\u80fa\u805a\u91a3\uff0c\u662f\u7d30\u80de\u5916\u57fa\u8cea(extracellular matrix)\u7684\u4e3b\u8981\u6210\u5206\u3002\u5b83\u53c3\u8207\u8a31\u591a\u751f\u7269\u904e\u7a0b\uff0c\u5305\u62ec\u7d50\u69cb\u652f\u6301(structural support)\u3001\u7d30\u80de\u9077\u79fb(cell migration)\u548c\u7d44\u7e54\u66f4\u65b0(tissue turnover)\u3002\u5728\u4eba\u985e\u4e2d\uff0c\u6709\u516d\u7a2e\u5df2\u77e5\u7684 HYAL\uff0c\u5b58\u5728\u65bc\u813e\u81df(spleen)\u3001\u76ae\u819a(skin)\u3001\u773c\u775b(eyes)\u3001\u809d\u81df(liver)\u3001\u814e\u81df(kidneys)\u3001\u5b50\u5bae(uterus)\u3001\u80ce\u76e4(placenta)\u7b49\u5668\u5b98\u548c\u553e\u6db2(saliva)\u3001\u773c\u6dda(tears)\u3001\u8840\u6db2(blood)\u3001\u7cbe\u6db2(semen)\u7b49\u9ad4\u6db2\u4e2d\u3002\u5b83\u5011\u589e\u52a0\u819c\u901a\u900f\u6027\uff0c\u4e26\u4f7f\u7d44\u7e54\u66f4\u5bb9\u6613\u6ef2\u900f\u3002\u7136\u800c\uff0cHYAL \u4e5f\u8207\u8a31\u591a\u5be6\u9ad4\u7624\u985e\u578b(solid tumor types)\u7684\u9032\u5c55\u6709\u95dc\uff0c\u5305\u62ec\u7d50\u76f4\u8178\u764c(colorectal)\u3001\u8180\u80f1\u764c(bladder)\u3001\u524d\u5217\u817a\u764c(prostate)\u3001\u4e73\u817a\u764c(breast)\u548c\u8166\u764c(brain cancer)\u3002\u6b64\u5916\uff0cHYAL \u5df2\u7528\u65bc\u773c\u79d1\u624b\u8853(ophthalmic surgery)\u548c\u76ae\u819a\u75c5\u5b78(brain cancer)\u3002 BioVision \u7684\u900f\u660e\u8cea\u9178\u9176\u6d3b\u6027 ELISA \u8a66\u5291\u76d2(Hyaluronidase Activity ELISA Kit)\u662f\u4e00\u7a2e\u9ad8\u901a\u91cf\u3001\u5b9a\u91cf\u7684\u514d\u75ab\u6e2c\u5b9a\uff0c\u65e8\u5728\u78ba\u5b9a\u5404\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d HYAL \u7684\u6d3b\u6027\u3002\u8207\u50b3\u7d71\u7684\u6fc1\u5ea6\u6e2c\u5b9a\u76f8\u6bd4\uff0c\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u3001\u9748\u654f\u4e14\u65b9\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u91cf\u751f\u7269\u6a23\u54c1\u4e2d HYAL \u7684\u6d3b\u6027\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u5c07\u6a23\u54c1\u6dfb\u52a0\u5230\u9810\u5148\u5857\u6709\u751f\u7269\u7d20-HA \u7684 96 \u5b54\u677f\u7684\u5b54\u4e2d\u3002\u5982\u679c\u6a23\u54c1\u5177\u6709\u4efb\u4f55 HYAL \u6d3b\u6027\uff0c\u5b83\u5c07\u8207\u677f\u4e0a\u7684\u751f\u7269\u7d20-HA \u7d50\u5408\uff0c\u56e0\u6b64\u6d17\u6ecc\u5f8c\u53ef\u7528\u7684\u6e38\u96e2\u751f\u7269\u7d20-HA \u4f4d\u9ede\u5c07\u6e1b\u5c11\u3002\u7136\u5f8c\u4f7f\u7528\u5728 OD 450 nm \u8655\u6e2c\u91cf\u7684\u7279\u5b9a\u93c8\u9ef4\u89aa\u548c\u7d20\u63a2\u91dd\u6aa2\u6e2c\u751f\u7269\u7d20-HA\u3002\u5438\u5149\u5ea6\u4fe1\u865f\u8207\u6a23\u54c1\u4e2d\u7684 HYAL \u6d3b\u6027\u6210\u53cd\u6bd4\u3002\u8a72\u8a66\u5291\u76d2\u53ef\u5728\u6e2c\u5b9a\u689d\u4ef6\u4e0b\u6aa2\u6e2c\u4f4e\u81f3 0.5 mU \u7684 HYAL \u6d3b\u6027\u3002<\/p>\n

\"\"<\/p>\n

\"\u539f\u5ee0\u64cd\u4f5c\u4e0b\u8f09\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nE4909-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nBioVision\u2019s Hyaluronidase Activity ELISA Kit is a high throughput, quantitative immunoassay desgined to determine the activity of HYALs in various biological samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance at 450 nm<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nDetermination of hyaluronidase activity in various biological samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u25cf The kit can detect as low as 0.5 mU of HYAL activity under the assay conditions.
\n\u25cf The kit offers a simple, sensitive and convenient method to measure the activity of HYALs in biological samples.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\nPre-coated 96 Well Strip Plate HYAL Assay Buffer HYAL Standard HRP Conjugate TMB Substrate Stop Solution Wash Buffer (10X) Conjugate Buffer Plate Sealers<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00baC<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e5\u0084\u0080\u00e5\u0099\u00a8\"\u8a66\u5291\u5fc5\u9808\u914d\u5408ELISA\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100<\/a> (\u57fa\u672c\u6b3e405\u3001450\u3001492\u3001630 nm \/\u9032\u968e\u6b3e\u53ef\u64f4\u589e\u81f37\u500b\u6ffe\u7247) \u4f7f\u7528\u3002\u660e\u8cea\u9178\u9176\u6d3b\u6027 ELISA \u8a66\u5291\u76d2(\u8ca8\u865fE4909-100)\u6e2c\u91cf\u65b9\u6cd5\u70ba\u5438\u6536\u5149450nm\u3002<\/p>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u900f\u660e\u8cea\u9178\u9176\u6d3b\u6027 ELISA \u8a66\u5291\u76d2 | \u00a0\u8ca8\u865fE4909-100 Hyaluronidase Activity […]<\/p>\n","protected":false},"author":3,"featured_media":28499,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1068],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28494"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28494"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28494\/revisions"}],"predecessor-version":[{"id":28504,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28494\/revisions\/28504"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28499"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28494"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28494"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28494"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}