{"id":28175,"date":"2021-07-22T13:26:43","date_gmt":"2021-07-22T05:26:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28175"},"modified":"2022-07-19T14:06:41","modified_gmt":"2022-07-19T06:06:41","slug":"%e9%88%a3%e8%aa%bf%e8%9b%8b%e7%99%bd-%e7%93%8a%e8%84%82%e7%b3%96%e7%8f%a0calmodulin-sepharose-beads-%e8%b2%a8%e8%99%9f7934","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=28175","title":{"rendered":"\u9223\u8abf\u86cb\u767d-\u74ca\u8102\u7cd6\u73e0(Calmodulin-Sepharose Beads) | \u8ca8\u865f7934"},"content":{"rendered":"

\u9223\u8abf\u86cb\u767d-\u74ca\u8102\u7cd6\u73e0(Calmodulin-Sepharose Beads<\/em><\/strong>) | \u00a0\u8ca8\u865f<\/strong>7934<\/strong><\/a><\/span><\/p>\n

Calmodulin-Sepharose Beads<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39:\u9223\u8abf\u86cb\u767d(Calmodulin)\u662f\u4e00\u7a2e\u9ad8\u5ea6\u4fdd\u5b88\u7684\u8abf\u7bc0\u6027\u771f\u6838\u86cb\u767d\uff0c\u53c3\u8207\u5404\u7a2e\u7d30\u80de\u904e\u7a0b\uff0c\u5982\u7cd6\u539f\u4ee3\u8b1d(glycogen metabolism)\u3001\u7d30\u80de\u9aa8\u67b6\u63a7\u5236(cytoskeletal control)\u3001\u795e\u7d93\u50b3\u905e(neurotransmission)\u3001\u78f7\u9178\u9e7d\u6d3b\u6027(phosphate activity)\u548c NAD\/NADP \u7684\u63a7\u5236\u3002\u9223\u8abf\u86cb\u767d(Calmodulin)\u7684\u591a\u7a2e\u529f\u80fd\u4f9d\u8cf4\u65bc\u5176\u8207\u5927\u91cf\u9776\u86cb\u767d\/\u9176\u76f8\u4e92\u4f5c\u7528\u4e26\u8abf\u7bc0\u5176\u6d3b\u6027\u7684\u80fd\u529b\u3002\u9223\u8abf\u86cb\u767d(Calmodulin)\u901a\u5e38\u901a\u904e\u5176\u758f\u6c34\u4f4d\u9ede\u8207\u86cb\u767d\u8cea\u7d50\u5408\uff0c\u9019\u4e9b\u4f4d\u9ede\u56e0 Ca2+ \u5b58\u5728\u6642\u7684\u69cb\u8c61\u8b8a\u5316\u800c\u66b4\u9732\u3002\u7136\u800c\uff0c\u4e00\u4e9b\u9776\u86cb\u767d\u8207\u9223\u8abf\u86cb\u767d\u4ee5\u4e0d\u4f9d\u8cf4\u9223\u7684\u65b9\u5f0f\u76f8\u4e92\u4f5c\u7528\u3002\u9223\u8abf\u86cb\u767d-\u74ca\u8102\u7cd6\u73e0(. Calmodulin-Sepharose beads)\u5141\u8a31\u5f9e\u751f\u7269\u6a23\u54c1\u4e2d\u7279\u7570\u6027\u5bcc\u96c6\u5df2\u77e5\u6216\u672a\u77e5\u7684\u9223\u8abf\u86cb\u767d\u7d50\u5408\u86cb\u767d\u3002\u6b64\u5916\uff0c\u9223\u8abf\u86cb\u767d-\u74ca\u8102\u7cd6\u73e0\u70ba\u9223\u8abf\u86cb\u767d\u7d50\u5408\u80bd (CBP) \u6a19\u8a18\u7684\u91cd\u7d44\u86cb\u767d\u7684\u89aa\u548c\u7d14\u5316\u63d0\u4f9b\u4e86\u65b9\u4fbf\u7684\u5de5\u5177\u3002 CBP \u6a19\u8a18\u86cb\u767d\u7684\u6d17\u812b\u53ef\u4ee5\u901a\u904e\u7c21\u55ae\u5730\u6dfb\u52a0\u87af\u5408\u914d\u9ad4(chelation ligand )\/\u53bb\u9664 Ca2+ \u4f86\u5be6\u73fe\u3002<\/p>\n

\u61c9\u7528\uff1a<\/p>\n

1. \u55ae\u4e00\u6b65\u9a5f\u7d14\u5316\u5929\u7136\u9223\u8abf\u7d20\u7d50\u5408\u86cb\u767d(native calmodulin-binding proteins)\u3002<\/p>\n

2. \u4e32\u806f\u89aa\u548c\u7d14\u5316 (Tandem affinity purification , TAP)\u86cb\u767d\u8cea\u8907\u5408\u7269\u7684<\/p>\n

3. CBP \u6a19\u8a18\u86cb\u767d(CBP tagged proteins)\u7684\u7d14\u5316\u3002<\/p>\n

4. \u771f\u6838\u7d30\u80de\u4e2d\u7d14\u5316\u9223\u8abf\u86cb\u767d\u8abf\u7bc0\u86cb\u767d(calmodulin-regulated proteins)<\/p>\n\n\n\n\n\n\n
Highlights<\/strong><\/td>\nFORMULATION: Supplied as 50% aqueous slurry in 20 % Ethanol.
\nLIGAND DENSITY: ~ 1.5 mg Calmodulin protein per ml of drained resin\/beads.
\nPREPARATION: Calmodulin-Sepharose Beads are prepared by covalently coupling recombinant human calmodulin (Cat # 7838) to 6% cross linked Sepharose beads.
\nAPPLICATIONS:
\n\u2022 Single-step purification of native calmodulin-binding proteins.
\n\u2022 Tandem affinity purification (TAP) of protein complexes.
\n\u2022 Purification of CBP tagged proteins.
\n\u2022 Purification of calmodulin-regulated proteins from all eukaryotic cells.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Eli J. Rogers, An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors.\u00a0Front Mol Neurosci, Feb 2019;\u00a0\u00a030842726.<\/a>\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u9223\u8abf\u86cb\u767d-\u74ca\u8102\u7cd6\u73e0(Calmodulin-Sepharose Beads) | \u00a0\u8ca8\u865f7934 Calmodu […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1096],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28175"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28175"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28175\/revisions"}],"predecessor-version":[{"id":28183,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28175\/revisions\/28183"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28175"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28175"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28175"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}