{"id":27805,"date":"2021-06-09T11:25:47","date_gmt":"2021-06-09T03:25:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27805"},"modified":"2022-07-19T14:13:10","modified_gmt":"2022-07-19T06:13:10","slug":"%e8%84%82%e8%82%aa%e7%b5%84%e7%b9%94%e5%9f%b9%e9%a4%8a%e8%84%82%e8%82%aa%e7%b4%b0%e8%83%9e%e6%a0%b8%e5%92%8c%e7%b4%b0%e8%83%9e%e8%b3%aa%e5%88%86%e9%9b%a2%e8%a9%a6%e5%8a%91%e7%9b%92-invent%e8%b2%a8","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=27805","title":{"rendered":"\u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2| Invent\u8ca8\u865fAN-029"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>:<\/strong> \u7528\u65bc\u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2\uff0820 \u500b\u53cd\u61c9\uff09| Invent<\/strong>\u8ca8\u865f<\/strong>AN-029<\/a>\u00a0\uff0820 \u500b\u53cd\u61c9\uff09<\/span><\/p>\n

Minute\u2122 Single Nuclei and Cytosol Isolation Kit for Adipose Tissues\/Cultured Adipocytes (20 preps)<\/p>\n

\u7522\u54c1\u4ecb\u7d39: \u8102\u80aa\u7d30\u80de(Adipocytes)\u662f\u9ad4\u5167\u4e3b\u8981\u7684\u80fd\u91cf\u5132\u5b58\u90e8\u4f4d\uff0c\u5b83\u5011\u4e5f\u5177\u6709\u91cd\u8981\u7684\u5167\u5206\u6ccc(endocrine)\u529f\u80fd\u3002\u56e0\u6b64\uff0c\u4e86\u89e3\u8102\u80aa\u7d30\u80de\u7684\u767c\u80b2(development)\u548c\u529f\u80fd(function)\u5c0d\u65bc\u4e86\u89e3\u751f\u7406(physiological)\u548c\u75c5\u7406(pathological)\u689d\u4ef6\u4e0b\u7684\u4ee3\u8b1d\u7a69\u614b(metabolic homeostasis)\u81f3\u95dc\u91cd\u8981\u3002\u5c07\u7d30\u80de\u6210\u5206\u5206\u6210\u55ae\u6838(single nucleus)\u548c\u7d30\u80de\u8cea(cytosolic fraction)\u90e8\u5206\u662f\u5be6\u9a57\u5ba4\u4e2d\u7684\u5e38\u898b\u505a\u6cd5\u3002\u7136\u800c\uff0c\u7576\u6d89\u53ca\u5230\u8102\u80aa\u7d30\u80de(adipocytes)\u6642\uff0c\u7531\u65bc\u8102\u80aa\u7d30\u80de\u7684\u8102\u6ef4(lipid droplets)\u6fc3\u5ea6\u9ad8\u548c\u86cb\u767d\u8cea\u542b\u91cf\u4f4e\uff0c\u56e0\u6b64\u5206\u96e2\u9019\u5169\u500b\u90e8\u5206\u8981\u56f0\u96e3\u5f97\u591a\u3002\u6587\u737b\u4e2d\u5831\u5c0e\u7684\u65b9\u6cd5\u65e2\u4e4f\u5473\u53c8\u8017\u6642\uff0c\u4e26\u4e14\u9700\u8981\u591a\u9054 50 \u514b\u7684\u7d44\u7e54\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5f9e\u8102\u80aa\u7d44\u7e54(adipose tissues)\u4e2d\u7372\u5f97\u9ad8\u7d14\u5ea6\u55ae\u6838(single nuclei)\u7684\u975e\u5e38\u5feb\u901f\u548c\u7c21\u55ae\u7684\u65b9\u6cd5\uff0c\u6700\u91cd\u8981\u7684\u662f\uff0c\u53ea\u9700\u8981\u6beb\u514b\u91cf\u7684\u7d44\u7e54\uff0c\u5c31\u53ef\u4ee5\u5f9e\u5c0f\u52d5\u7269\u548c\u6d3b\u6aa2\u6a23\u672c\u4e2d\u5206\u96e2\u7d30\u80de\u6838(nuclei)\u548c\u7d30\u80de\u8cea(cytosol)\u3002\u5206\u96e2\u51fa\u7684\u5b8c\u6574\u7d30\u80de\u6838\u6709\u8a31\u591a\u61c9\u7528\uff0c\u4f8b\u5982\u55ae\u6838 RNA \u6e2c\u5e8f(single nucleus RNA sequencing)\u3001SDS-PAGE()\u3001\u514d\u75ab\u5370\u8de1(immunoblotting)\u3001ELISA\u3001IP\u3001\u86cb\u767d\u8cea\u5b9a\u4f4d(protein localization)\u3001\u51dd\u81a0\u9077\u79fb\u7387\u8b8a\u5316\u6e2c\u5b9a(gel mobility shift assays)\u30012-D \u51dd\u81a0(2-D gels)\u548c\u5176\u4ed6\u61c9\u7528\u3002<\/p>\n

\"\"<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

\"\"<\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

1. Rajbhandari, P., Arneson, D., Feng, A. C., Ahn, I. S., Diamante, G., Zaghari, N., … & Smale, S. T. (2019). Single Cell Analysis Reveals Immune Cell-Adipocyte Crosstalk Regulating the Transcription of Thermogenic Adipocytes.\u00a0bioRxiv<\/em>, 669853.<\/p>\n

Kit includes:<\/strong><\/p>\n\n\n\n\n\n\n\n\n
Items<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
N\/C Buffer<\/td>\n15 ml<\/td>\n<\/tr>\n
1.5 ml Eppendorf Tubes<\/td>\n20 units<\/td>\n<\/tr>\n
Pestle for 1.5 ml Eppendorf Tubes<\/td>\n2 units<\/td>\n<\/tr>\n
Filter Cartridge<\/td>\n20 units<\/td>\n<\/tr>\n
2 ml Collection Tubes<\/td>\n20 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/u><\/td>\nCatalog No.<\/u><\/td>\n<\/tr>\n
\u3000<\/u><\/td>\n\u3000<\/u><\/td>\n<\/tr>\n
Minute TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\nSD-001\/SN-002<\/td>\n<\/tr>\n
Minute TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\nSC-003<\/td>\n<\/tr>\n
Minute TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\nSN-006<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\nSM-005<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\nSM-005-p<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\nMP-007<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests) NEW!<\/td>\nMM-038<\/td>\n<\/tr>\n
Minute TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\nSD-008\/SN-009<\/td>\n<\/tr>\n
Minute TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\nSN-010<\/td>\n<\/tr>\n
Minute TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\nCP-011<\/td>\n<\/tr>\n
Minute TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\nSB-004<\/td>\n<\/tr>\n
Minute TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\nSC-012<\/td>\n<\/tr>\n
Minute TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\nNE-013<\/td>\n<\/tr>\n
Minute TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\nHP-014<\/td>\n<\/tr>\n
Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYT-015<\/td>\n<\/tr>\n
Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYD-016<\/td>\n<\/tr>\n
Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\nYM-017<\/td>\n<\/tr>\n
Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\nMM-018<\/td>\n<\/tr>\n
Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\nPN-019<\/td>\n<\/tr>\n
MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\nHD-021<\/td>\n<\/tr>\n
MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\nAT-022<\/td>\n<\/tr>\n
MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\nAF-023<\/td>\n<\/tr>\n
MinuteTM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\nNI-024<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps) NEW!<\/td>\nBN-020<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps) NEW!<\/td>\nSN-047<\/td>\n<\/tr>\n
MinuteTM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\nFE-025<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\nEI-027<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\nSE-030<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\nNT-032<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\nLY-034<\/td>\n<\/tr>\n
MinuteTM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\nER-036<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-039<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-042<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPT-040<\/td>\n<\/tr>\n
MinuteTM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPN-041<\/td>\n<\/tr>\n
MinuteTM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW!<\/td>\nPF-045<\/td>\n<\/tr>\n
MinuteTM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests) NEW!<\/td>\nSV-043<\/td>\n<\/tr>\n
MinuteTM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW!<\/td>\nML-044<\/td>\n<\/tr>\n
MinuteTM<\/sup> Rubisco Depletion Kit (20 tests) NEW!<\/td>\nRD-046<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\nSA-01-SK<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\nSA-02-BT<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\nSA-03-BV<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\nSA-04-TD<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\nSA-05-IM<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\nSA-06-MS<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\nSA-07-IS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u7522\u54c1\u540d\u7a31: \u7528\u65bc\u8102\u80aa\u7d44\u7e54\/\u57f9\u990a\u8102\u80aa\u7d30\u80de\u6838\u548c\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2\uff0820 \u500b\u53cd\u61c9\uff09| Invent\u8ca8\u865fAN-029\u00a0\uff08 […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1125],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27805"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27805"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27805\/revisions"}],"predecessor-version":[{"id":27808,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27805\/revisions\/27808"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27805"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27805"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27805"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}