{"id":27253,"date":"2021-04-28T14:43:40","date_gmt":"2021-04-28T06:43:40","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27253"},"modified":"2024-05-10T16:58:35","modified_gmt":"2024-05-10T08:58:35","slug":"qpcr-adeno-associated-virus-titration-titer-kit-aav%e7%97%85%e6%af%92%e5%bf%ab%e9%80%9f%e7%b4%94%e5%8c%96%e5%a5%97%e7%b5%84g931","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=27253","title":{"rendered":"qPCR Adeno-Associated Virus Titration (Titer) Kit | AAV\u75c5\u6bd2\u5feb\u901f\u7d14\u5316\u5957\u7d44G931"},"content":{"rendered":"

qPCR Adeno-Associated Virus Titration (Titer) Kit\u00a0<\/strong>\u75c5\u6bd2\u5feb\u901f\u5b9a\u91cf\u5957\u7d44\u00a0 (\u4e0d\u9700\u8981\u984d\u5916\u7684\u88c2\u89e3)\u00a0<\/span><\/strong><\/p>\n

\"G931<\/p>\n

abm\u7684qPCR AAV\u6ef4\u5b9a\u8a66\u5291\u76d2\u662f\u901a\u904eqPCR\u9032\u884c\u7684\u4e00\u6b65\u5206\u6790\uff0c\u4e0d\u9700\u8981\u984d\u5916\u7684\u88c2\u89e3\uff0c\u53ef\u4ee5\u5728\u4e00\u5c0f\u6642\u5167\u5b8c\u6210\u3002\u8207\u540c\u985e\u8a66\u5291\u76d2\u76f8\u6bd4\uff0c\u8a72\u8a66\u5291\u76d2\u65e8\u5728\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\uff0c\u53ef\u78ba\u4fdd\u6700\u5c0f\u7684\u975e\u7279\u7570\u6027\u80cc\u666f\u548c\u66f4\u597d\u7684\u6574\u9ad4\u6027\u80fd\u3002 ROX\u53c3\u8003\u67d3\u6599\u8207MasterMix\u5206\u958b\u63d0\u4f9b\uff0c\u4f7f\u8a72\u8a66\u5291\u76d2\u8207\u5927\u591a\u6578qPCR\u5100\u5668\u901a\u7528\u517c\u5bb9\u3002<\/p>\n

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qPCR Adeno-Associated Virus Titration (Titer) Kit-G931<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n
PRODUCT COMPONENT<\/strong><\/th>\nQUANTITY<\/strong><\/th>\n<\/tr>\n<\/thead>\n
BlasTaq\u2122 2X qPCR Titer MasterMix<\/td>\n1.25 ml<\/td>\n<\/tr>\n
Primer Mix<\/td>\n100 rxn (200 \u00b5l)<\/td>\n<\/tr>\n
Standard Control DNA<\/td>\n50 \u00b5l<\/td>\n<\/tr>\n
DNase I Reaction Mix<\/td>\n2 x 1.0 ml<\/td>\n<\/tr>\n
ROX Reference Dye<\/td>\n15 \u00b5l<\/td>\n<\/tr>\n
Nuclease-Free H2<\/sub>O<\/td>\n2 x 1.0 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

abm<\/strong>\u2019s\u00a0qPCR AAV Titer Kit<\/strong>\u00a0is a one-step assay by qPCR which does not require additional lysis, and can be completed under one hour. Designed to deliver\u00a0high sensitivity and specificity<\/strong>, the kit ensures minimal non-specific background and better overall performance compared to similar kits. ROX reference dye is provided separate from the MasterMix, making this kit universally compatible with most qPCR instruments.<\/p>\n

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Data Analysis Plot Ct value (Y-axis, linear scale) vs. Virus titer (X-axis, logarithmic scale). Generate a logarithmic regression using the four (4) Standard Control DNA dilutions to determine the unknown virus sample titer using y = mx + b from the trendline equation. The R2 value should be > 0.95 to justify the proper assay setup. Note to include the dilution factor in the final calculation (i.e. if you diluted your purified viral sample 1\/100 in Step 1 and 1\/10 in Step 2, then the titer of the unknown sample should be multiplied by a factor of 100*10).<\/p>\n\n\n\n\n\n\n\n
MACHINE TYPE<\/th>\nMACHINE<\/th>\n<\/tr>\n<\/thead>\n
High ROX Machines<\/td>\n\n
    \n
  • ABI\u00a0\u00ae 7000, 7300, 7700, 7900, 7900HT, StepOnePlus\u2122, StepOne\u2122, OpenArray, PRISM\u2122 Sequencing Detection Series<\/li>\n
  • Biometra TOptical<\/li>\n
  • Fluidigm BioMark\u2122<\/li>\n
  • Wafergene SmartChip System<\/li>\n
  • TianLong TL998 System<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Low ROX Machines<\/td>\n\n
    \n
  • ABI\u00ae 7500, 7500 Fast, Viia\u2122 7, QuantStudio, QuantStudio 3\/5\/6\/7<\/li>\n
  • BioGene InSyte\u2122<\/li>\n
  • Illumina Eco<\/li>\n
  • Analytikjena qTower Series<\/li>\n
  • Stratagene\u00ae Mx3000, Mx3005, Mx4000<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
No ROX machines<\/td>\n\n