{"id":27084,"date":"2021-03-18T17:13:04","date_gmt":"2021-03-18T09:13:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27084"},"modified":"2022-07-19T14:36:02","modified_gmt":"2022-07-19T06:36:02","slug":"mouse-anti-human-igg4-pfc-hrp%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f9190-05-%e8%a6%8f%e6%a0%bc1-0-ml","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=27084","title":{"rendered":"Mouse Anti-Human IgG4 pFc’-HRP\u6297\u9ad4 | \u8ca8\u865f9190-05 \u898f\u683c1.0 mL"},"content":{"rendered":"

Mouse Anti-Human IgG4 pFc’-HRP\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong>9190-05 <\/strong>\u898f\u683c<\/strong>1.0 mL<\/a><\/span><\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\"\"<\/p>\n

ELISA plate was coated with purified human IgG1, IgG2, IgG3, and IgG4. Immunoglobulins were detected with serially diluted Mouse Anti-Human IgG4 pFc\u2019-HRP (SB Cat. No. 9190-05).<\/p>\n

\u4e8c\u7d1a\u6297\u9ad4 (Secondary antibodies) \u61c9\u7528:<\/p>\n

\u4e8c\u6297(Secondary antibodies)\u662f\u91dd\u5c0d\u514d\u75ab\u7403\u86cb\u767d\uff08immunoglobulin , Ig\uff09\u5206\u5b50\u7684\u591a\u514b\u9686(polyclonal) \u548c\u55ae\u514b\u9686\u6297\u9ad4(monoclonal antibodies)\u3002\u5b83\u5011\u901a\u5e38\u8207\u6297\u539f\u7279\u7570\u6027\u4e00\u6297(specific primary antibody) \u4e00\u8d77\u4f7f\u7528\uff0c\u4e26\u4e14\u901a\u5e38\u88ab\u6a19\u5b9a\u9175\u7d20\u6216\u87a2\u5149\u7269\u8cea\u4ee5\u5e6b\u52a9\u6aa2\u6e2c\u3002\u4e8c\u6297\u53ef\u7528\u65bc\u591a\u7a2e\u61c9\u7528\uff0c\u4f8b\u5982ELISA\u3001\u514d\u75ab\u5370\u8de1(Immunoblotting)\u3001\u6d41\u5f0f\u7d30\u80de\u5100(Flow Cytometry)\u3001\u514d\u75ab\u7d44\u7e54\u67d3\u8272(Immunohistochemistry)\u3001\u514d\u75ab\u7d30\u80de\u5316\u5b78(Immuocytochemistry)\u3002\u4e8c\u7d1a\u6297\u9ad4\u901a\u5e38\u7528\u9176\u6216\u87a2\u5149\u67d3\u6599\u6a19\u8a18\uff0c\u9019\u4f7f\u5f97\u80fd\u5920\u901a\u904e\u6bd4\u8272\uff0c\u5316\u5b78\u767c\u5149\u548c\u87a2\u5149\u6280\u8853\u9032\u884c\u6aa2\u6e2c\u3002\u4f8b\u5982\uff0c\u9176\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\uff08enzymes horseradish peroxidase , HRP\uff09\u548c\u9e7c\u6027\u78f7\u9178\u9176\uff08alkaline phosphatase , AP\uff09\u901a\u904e\u6bd4\u8272\u548c\u5316\u5b78\u767c\u5149\u5e73\u53f0\u5728ELISA\u548c\u86cb\u767d\u8cea\u5370\u8de1\u61c9\u7528\u4e2d\u662f\u6709\u50f9\u503c\u7684\u3002\u87a2\u5149\u86cb\u767d\u8af8\u5982\u87a2\u5149\u7d20\uff08fluorescein , FITC\uff09\uff0c\u85fb\u7d05\u86cb\u767d\uff08phycoerythrin , PE\uff09\u548c\u82b1\u9752\uff08cyanine , CY\uff09\u7684\u87a2\u5149\u67d3\u6599\u5728\u87a2\u5149\u7684\u61c9\u7528\u4e2d\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\uff0c\u5305\u62ec\u6d41\u5f0f\u7d30\u80de\u8853\uff0c\u514d\u75ab\u87a2\u5149\u986f\u5fae\u8853\u548c\u591a\u91cd\u6e2c\u5b9a\u3002\u4e8c\u6297\u5141\u8a31\u6a19\u8a18\u548c\u6aa2\u6e2c\u7684\u9748\u6d3b\u6027\u4ee5\u53ca\u901a\u904e\u4fe1\u865f\u653e\u5927\uff0c\u53ef\u589e\u52a0\u7684\u9748\u654f\u5ea6\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Mouse Anti-Human IgG4 pFc’-HRP\u6297\u9ad4\uff0c\u5177HRP\u6a19\u8a8c\u3002\u91dd\u5c0dHuman IgG4<\/sub>\u00a0pFc’\u5177\u7279\u7570\u6027\u3002\u514d\u75ab\u4f86\u6e90\u70baHuman IgG4 myeloma protein\u3002<\/p>\n

Isotype\u70baMouse (BALB\/c) IgG3<\/sub>\u03ba\u3002Isotype Control Mouse IgG3<\/sub>-HRP<\/a>\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u572850% Glycerol\/50% Phosphate buffered saline, pH 7.4\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\nHP6023<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse (BALB\/c) IgG3<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG3<\/sub>-HRP<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nHuman IgG4<\/sub>\u00a0myeloma protein<\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nHuman IgG4<\/sub>\u00a0pFc’<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nHRP (Horseradish Peroxidase)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\n50% Glycerol\/50% Phosphate buffered saline, pH 7.4<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C
\nPlease refer to product specific\u00a0SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

ELISA \u2013 Quality tested\u00a02-9<\/sup>
\nFLISA \u2013 Quality tested
\nFlow Cytometry \u2013 Reported in literature\u00a012-14<\/sup>
\nImmunohistochemistry-Frozen Sections \u2013 Reported in literature\u00a010<\/sup>
\nWestern Blot \u2013 Reported in literature\u00a011<\/sup>
\nImmunoprecipitation \u2013 Reported in literature\u00a01<\/sup>
\nSurface Plasmon Resonance \u2013 Reported in literature\u00a015<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific\u00a0Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2796684<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

References<\/p>\n

    \n
  1. Reimer CB, Phillips DJ, Aloisio CH, Moore DD, Galland GG, Wells TW, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984;3:263-75. (Immunogen, IP)<\/li>\n
  2. Tomana M, Novak J, Julian BA, Matousovic K, Konecny K, Mestecky J. Circulating immune complexes in IgA nephropathy consist of IgA1 with galactose-deficient hinge region and antiglycan antibodies. J Clin Invest. 1999;104:73-81. (ELISA)<\/li>\n
  3. Whary MT, Sundina N, Bravo LE, Correa P, Quinones F, Caro F, et al. Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesis. Cancer Epidemiol Biomarkers Prev. 2005;14:1464-9. (ELISA)<\/li>\n
  4. Sabbatini P, Tsuji T, Ferran L, Ritter E, Sedrak C, Tuballes K, et al. Phase I trial of overlapping long peptides from a tumor self-antigen and poly-ICLC shows rapid induction of integrated immune response in ovarian cancer patients. Clin Cancer Res. 2012;18:6497-508. (ELISA)<\/li>\n
  5. Itoh M, Weerasooriya MV, Yahathugoda TC, Takagi H, Samarawickrema WA, Nagaoka F, et al. Effects of 5 rounds of mass drug administration with diethylcarbamazine and albendazole on filaria-specific IgG4 titers in urine: 6-year follow-up study in Sri Lanka. Parasitol Int. 2011;60:393-7. (ELISA)<\/li>\n
  6. Olaru F, Wang X, Luo W, Ge L, Miner JH, Kleinau S, et al. Proteolysis breaks tolerance toward intact \u03b1345(IV) collagen, eliciting novel anti-glomerular basement membrane autoantibodies specific for \u03b1345NC1 hexamers. J Immunol. 2013;190:1424-32. (ELISA)<\/li>\n
  7. Ferreira AR, Singh B, Cabrera-Mora M, Magri De Souza AC, Queiroz Marques MT, Porto LC, et al. Evaluation of naturally acquired IgG antibodies to a chimeric and non-chimeric recombinant species of Plasmodium vivax reticulocyte binding protein-1: lack of association with HLA-DRB1*\/DQB1* in malaria exposed individuals from the Brazilian Amazon. PLoS One. 2014;9(8):e105828. (ELISA)<\/li>\n
  8. Haag S, Schneider N, Mason DE, Tuncel J, Andersson IE, Peters EC, et al. Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. Arthritis Rheumatol. 2014;66:1440-9. (ELISA)<\/li>\n
  9. Lin J, Markowitz GS, Valeri AM, Kambham N, Sherman WH, Appel GB, et al. Renal monoclonal immunoglobulin deposition disease: the disease spectrum. J Am Soc Nephrol. 2001;12:1482-92. (IHC-FS)<\/li>\n
  10. de Vos AF, Melief M, van Riel D, Boon L, van Eijk M, de Boer M, et al. Antagonist anti-human CD40 antibody inhibits germinal center formation in cynomolgus monkeys. Eur J Immunol. 2004;34:3446-55. (IHC-FS)<\/li>\n
  11. Fischer PU, Curtis KC, Folk SM, Wilkins PP, Marcos LA, Weil GJ. Serological diagnosis of North American Paragonimiasis by Western blot using Paragonimus kellicotti adult worm antigen. Am J Trop Med Hyg. 2013;88:1035-40. (WB)<\/li>\n
  12. Liles WC, Dale DC, Price TH, Gaviria JM, Turner T, Saoud J, et al. Inhibition of in vivo neutrophil transmigration by a novel humanized anti-CD11\/CD18 monoclonal antibody. Cytokines Cell Mol Ther. 2000;6:121-6. (FC)<\/li>\n
  13. Verbon A, Dekkers PE, ten Hove T, Hack CE, Pribble JP, Turner T, et al. IC14, an anti-CD14 antibody, inhibits endotoxin-mediated symptoms and inflammatory responses in humans. J Immunol. 2001;166:3599-605. (FC)<\/li>\n
  14. Toyoda C, Suzuki Y, Tsuneyama H, Onodera T, Masuno A, Yabe R, et al. Production of human monoclonal anti-Jk3, recognising an epitope including the Jka\/Jkb polymorphic site of the Kidd glycoprotein. Transfus Med. 2014;24:286-91. (FC)<\/li>\n
  15. Lewis KB, Hughes RJ, Epstein MS, Josephson NC, Kempton CL, Kessler CM, et al. Phenotypes of allo- and autoimmune antibody responses to FVIII characterized by surface plasmon resonance. PLoS One. 2013;8(5):e61120. (Surface Plasmon Resonance)<\/li>\n<\/ol>\n\n\n\n\n\n\n
    Gene ID<\/strong><\/td>\n3503<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
    Gene Symbol<\/strong><\/td>\nIGHG4<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
    Uniprot ID<\/strong><\/td>\nP01861<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
    Uniprot Name<\/strong><\/td>\nIGHG4_HUMAN<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Mouse Anti-Human IgG4 pFc’-HRP\u6297\u9ad4 | \u8ca8\u865f9190-05 \u898f\u683c1. […]<\/p>\n","protected":false},"author":3,"featured_media":18700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136,198,3],"tags":[1169],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27084"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27084"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27084\/revisions"}],"predecessor-version":[{"id":27085,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27084\/revisions\/27085"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18700"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27084"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27084"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27084"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}