{"id":27054,"date":"2021-03-17T11:32:54","date_gmt":"2021-03-17T03:32:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=27054"},"modified":"2022-07-19T14:37:37","modified_gmt":"2022-07-19T06:37:37","slug":"%e7%93%9c%e6%b0%a8%e9%85%b8citrulline%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-citrulline-fluorometric-assay-kit-%e8%b2%a8%e8%99%9fk2002-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=27054","title":{"rendered":"\u74dc\u6c28\u9178(Citrulline)\u5206\u6790\u8a66\u5291\u76d2 | Citrulline Fluorometric Assay Kit \u8ca8\u865fK2002-100"},"content":{"rendered":"

\u74dc\u6c28\u9178(Citrulline)\u5206\u6790\u8a66\u5291\u76d2 | Citrulline Fluorometric Assay Kit \u8ca8\u865f<\/strong>K2002-100<\/a><\/span><\/p>\n

\"\"<\/p>\n

\u74dc\u6c28\u9178\u5316(Citrullination)\u662f\u80bd\u7cbe\u6c28\u9178\u7cbe\u6c28\u9178\u53bb\u4e9e\u6c28\u9176\uff08peptidylarginine deiminases , PAD\uff09\u5c07\u6c28\u57fa\u9178\u7cbe\u6c28\u9178(amino acid arginine)\u8f49\u5316\u70ba\u9776\u86cb\u767d\u4e2d\u7684\u74dc\u6c28\u9178(Citrulline)\u3002\u74dc\u6c28\u9178(Citrulline)\u4e0d\u662f20\u7a2e\u6a19\u6e96\u6c28\u57fa\u9178\u4e4b\u4e00\uff0c\u800c\u662f\u7ffb\u8b6f\u5f8c\u4fee\u98fe(post-translational modification)\u7684\u7d50\u679c\u3002 \u86cb\u767d\u8cea\u7684\u74dc\u6c28\u9178\u5316\u5c0d\u65bc\u7dad\u6301\u6b63\u5e38\u7684\u751f\u7406\u529f\u80fd\u975e\u5e38\u91cd\u8981\uff0c\u5305\u62ec\u901a\u904e\u7d44\u86cb\u767d\u4fee\u98fe(histone modification)\uff0c\u514d\u75ab\u53cd\u61c9(immune responses)\u7b49\u8abf\u7bc0\u57fa\u56e0\u3002\u7136\u800c\uff0c\u74dc\u6c28\u9178\u5316(Citrullination)\u5ea6\u589e\u52a0\u8207\u514d\u75ab\u75c5\u7406\u72c0\u6cc1\u548c\u795e\u7d93\u7cfb\u7d71\u75be\u75c5, \u5982\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\uff0c\u591a\u767c\u6027\u786c\u5316\u75c7(multiple sclerosis)\u548c\u963f\u723e\u8328\u6d77\u9ed8\u6c0f\u75c5(Alzheimer\u2019s disease\uff09\u6709\u95dc\u3002 BioVision\u81ea\u8c6a\u5730\u63d0\u4f9b\u5168\u9762\u7684\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u86cb\u767d\u8cea\u548c\u9176\uff0c\u6297\u9ad4\u548c\u751f\u5316\u8a66\u5291\u7d44\u5408\uff0c\u4ee5\u4fc3\u9032\u74dc\u6c28\u9178\u5316\u7814\u7a76(citrullination research)\u3002<\/p>\n

for measuring total Citrulline concentrations<\/em><\/strong><\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: L-\u74dc\u6c28\u9178(L-Citrulline)\u662f\u4e00\u7a2e\u975e\u86cb\u767d\u534a\u5fc5\u9700\u6c28\u57fa\u9178(non-proteogenic, semi-essential amino acid)\u3002\u74dc\u6c28\u9178(Citrulline)\u901a\u904e\u9ce5\u6c28\u9178\u6c28\u57fa\u7532\u57fa\u8f49\u79fb\u9176(ornithine carbamoyltransferase)\u6216\u4f5c\u70ba\u4e00\u6c27\u5316\u6c2e\u5408\u9176\uff08Nitric oxide synthase ,NOS\uff09\u6d3b\u6027\u7684\u526f\u7522\u7269\u5f62\u6210\u3002 NOS\u53cd\u61c9\u4e2d\u7522\u751f\u7684\u74dc\u6c28\u9178(Citrulline)\u53ef\u901a\u904e\u5c3f\u7d20\u5faa\u74b0\u4e2d\u7684\u5169\u7a2e\u9176\u7cbe\u6c28\u7425\u73c0\u9178\u5408\u6210\u9176\uff08argininosuccinate synthetase , ASS\uff09\u548c\u7cbe\u6c28\u7425\u73c0\u9178\u88c2\u5408\u9176\uff08argininosuccinate lyase , ASL\uff09\u518d\u5faa\u74b0\u70ba\u7cbe\u6c28\u9178\u3002\u5df2\u8b49\u660e\u5728\u5177\u6709ASS\u6216ASL\u57fa\u56e0\u7a81\u8b8a\u7684\u60a3\u8005\u4e2d\u74dc\u6c28\u9178(Citrulline)\u6fc3\u5ea6\u5347\u9ad8\u3002\u897f\u74dc(Watermelon)\u662f\u74dc\u6c28\u9178(Citrulline)\u7684\u6700\u8c50\u5bcc\u4f86\u6e90\u4e4b\u4e00\u3002\u6b64\u5916\uff0c\u7531\u65bc\u74dc\u6c28\u9178(Citrulline)\u53c3\u8207\u4e00\u6c27\u5316\u6c2e\uff08NO\uff09\u7684\u5408\u6210\uff0c\u6709\u52a9\u65bc\u8840\u7ba1\u8212\u5f35\uff0c\u56e0\u6b64\u5df2\u88ab\u5ba3\u50b3\u70ba\u904b\u52d5\u71df\u990a\u88dc\u5145\u5291(sports nutrition supplement)\u3002 BioVision\u7684\u74dc\u6c28\u9178(Citrulline)\u5206\u6790\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u7279\u7570\u6027\u548c\u7c21\u4fbf\u7684\u65b9\u6cd5\u4f86\u6e2c\u91cf\u5404\u7a2e\u6a23\u54c1\u4e2d\u74dc\u6c28\u9178\u7684\u7e3d\u6fc3\u5ea6\u3002\u5728\u9019\u7a2e\u9176\u4fc3\u6e2c\u5b9a\u4e2d\uff0c\u74dc\u6c28\u9178\u8f49\u5316\u70ba\u4e00\u7cfb\u5217\u4e2d\u9593\u9ad4\uff0c\u9032\u4e00\u6b65\u8207\u63a2\u91dd\u53cd\u61c9\u7522\u751f\u7a69\u5b9a\u7684\u87a2\u5149\u4fe1\u865f\uff08Ex \/ Em = 535\/587 nm\uff09\u3002\u8a72\u5957\u4ef6\u7c21\u55ae\uff0c\u6613\u65bc\u57f7\u884c\uff0c\u9748\u654f\u4e26\u4e14\u5177\u6709\u9ad8\u901a\u91cf\u9069\u61c9\u6027\u3002\u5b83\u53ef\u4ee5\u6aa2\u6e2c\u51fa\u751f\u7269\u6a23\u54c1\u4e2d\u4f4e\u81f32 \u00b5M\u7684\u74dc\u6c28\u9178\u3002<\/p>\n

\"\"\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK2002-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nL-Citrulline is a non-proteogenic, semi-essential amino acid. Citrulline is formed either by ornithine carbamoyltransferase or as a byproduct of the Nitric oxide synthase (NOS) activity. Citrulline generated in the NOS reaction can be recycled to arginine by the two enzymes argininosuccinate synthetase (ASS) and argininosuccinate lyase (ASL) acting in the Urea Cycle. Citrulline concentrations have been shown to be elevated in patients with mutated ASS or ASL genes. Watermelon is one of the richest sources of Citrulline. Additionally, Citrulline has been advertised as sports nutrition supplement due to its involvement in Nitric Oxide (NO) synthesis, which helps with vasodilation. BioVision\u2019s Citrulline Assay Kit provides a rapid, specific, and easy method for the measurement of total Citrulline concentrations in a wide variety of samples. In this enzymatic assay, Citrulline is converted into a series of intermediates, which further reacts with a probe producing a stable fluorescent signal (Ex\/Em = 535\/587 nm). The kit is simple, easy to perform, sensitive and is highthroughput adaptable. It can detect as low as 2 \u00b5M Citrulline in biological samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em = 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nAll<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Analysis of Urea cycle and NO cycle
\n\u2022 Measurement of Citrulline in beverages and biological samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Easy method for the measurement of total Citrulline concentrations in a wide variety of samples.
\n\u2022 Specific
\n\u2022 Rapid<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\nCitrulline Developer Mix
\nCitrulline Enzyme Mix
\nCitrulline Cofactor Mix
\nCitrulline Converter Mix
\nCitrulline Buffer Supplement
\nCitrulline Assay Buffer
\nCitrulline Standard
\nCitrulline Probe<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u74dc\u6c28\u9178(Citrulline)\u5206\u6790\u8a66\u5291\u76d2 | Citrulline Fluorometric Assay Ki […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1177],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27054"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=27054"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27054\/revisions"}],"predecessor-version":[{"id":27060,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/27054\/revisions\/27060"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=27054"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=27054"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=27054"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}