{"id":26841,"date":"2021-01-15T11:56:02","date_gmt":"2021-01-15T03:56:02","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26841"},"modified":"2022-07-19T15:13:50","modified_gmt":"2022-07-19T07:13:50","slug":"sars-cov-2-s1%e8%9b%8b%e7%99%bd-ace2%e7%b5%90%e5%90%88%e6%8a%91%e5%88%b6%e5%8a%91%e7%af%a9%e9%81%b8%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk2050-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=26841","title":{"rendered":"SARS-CoV-2 S1\u86cb\u767d-ACE2\u7d50\u5408\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865fK2050-100"},"content":{"rendered":"

SARS-CoV-2 S1\u86cb\u767d-ACE2\u7d50\u5408\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K2050-100<\/a><\/span><\/p>\n

\u00a0<\/em><\/strong>\u7522\u54c1\u63cf\u8ff0:<\/strong> \u56b4\u91cd\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd22\uff08Severe Acute Respiratory Syndrome Coronavirus 2, SARS-CoV-2\uff09\u4e5f\u7a31\u70ba2019\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2\uff08coronavirus , 2019-nCoV\uff09\u6216\u4eba\u51a0\u72c0\u75c5\u6bd22019\uff08HCoV-19\u6216hCoV-19\uff09\uff0c\u662f\u5c0e\u81f42019\u5e74\u51a0\u72c0\u75c5\u6bd2\u75c5\uff08COVID\uff09\u7684\u539f\u56e0-19\uff09\u5927\u6d41\u884c\u3002\u5b83\u662f\u4e00\u7a2eRNA\u75c5\u6bd2(RNA virus)\uff0c\u53ef\u5f15\u8d77\u4eba\u985e\u56b4\u91cd\u7684\u547c\u5438\u9053\u75be\u75c5(severe respiratory diseases)\u3002 SARS-CoV-2\u51a0\u72c0\u75c5\u6bd2\u5305\u542b\u56db\u500b\u4e3b\u8981\u7d50\u69cb\u86cb\u767d(structural proteins)\uff0c\u5373\u523a\u7a81\uff08Spike , S\uff09\uff0c\u819c\uff08Membrane , M\uff09\uff0cEnvelope\uff08E\uff09\u548c\u6838\u6bbc\u86cb\u767d\uff08Envelope , N\uff09\u3002\u7a57\u86cb\u767d(Envelope)\u4f4d\u65bc\u75c5\u6bd2\u9ad4\u7684\u5916\u6bbc\u4e0a\u4e26\u4ecb\u5c0e\u75c5\u6bd2\u9032\u5165\uff0c\u56e0\u6b64\u5728\u8a98\u5c0e\u4e2d\u548c\u6297\u9ad4(neutralizing antibodies)\u548c\u4fdd\u8b77\u6027\u514d\u75ab(protective immunity)\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 S\u86cb\u767d\u7531S1\u548cS2\u4e9e\u57fa(subunits)\u7d44\u6210\u3002 S1\u4e9e\u57fa(S1 subunit)\u5305\u542b\u4e00\u500b\u53ef\u4ee5\u8207\u5bbf\u4e3b\u53d7\u9ad4\u7279\u7570\u6027\u7d50\u5408\u7684\u53d7\u9ad4\u7d50\u5408\u57df\uff0c\u5373\u8840\u7ba1\u7dca\u5f35\u7d20\u8f49\u5316\u91762\uff08Angiotensin Converting Enzyme 2, ACE2\uff09\uff0c\u5b83\u53ef\u4ee5\u4fc3\u9032\u75c5\u6bd2\u9032\u5165\u9776\u7d30\u80de\uff0c\u5305\u62ec\u547c\u5438\u9053(respiratory)\u6216\u8178\u9053\u4e0a\u76ae\u7d30\u80de(intestinal epithelial cells)\uff0c\u5167\u76ae\u7d30\u80de(endothelial cells)\uff0c\u80ba\u6ce1\u55ae\u6838\u7d30\u80de(alveolar monocytes)\u6216\u5de8\u566c\u7d30\u80de(macrophages)\u3002\u53d7\u9ad4\u8b58\u5225(receptor recognition)\u6b65\u9a5f\u662f\u75c5<\/strong>\u6bd2\u611f\u67d3\u6027(viral infectivity)\uff0c\u767c\u75c5\u6a5f\u7406(pathogenesis)\u548c\u5bbf\u4e3b\u7bc4\u570d(host range)\u7684\u91cd\u8981\u6c7a\u5b9a\u56e0\u7d20\u3002\u56e0\u6b64\uff0c\u91dd\u5c0dS1\u86cb\u767d\u548cACE2\u76f8\u4e92\u4f5c\u7528\u7684\u5e72\u9810\u7b56\u7565\u70ba\u5305\u62ec\u5c0f\u5206\u5b50(small molecules)\u548c\u6cbb\u7642\u6027\u6297\u9ad4(therapeutic antibodies)\u5728\u5167\u7684\u75ab\u82d7\u63a5\u7a2e(vaccination)\u6216\u6297\u75c5\u6bd2\u7b56\u7565\u63d0\u51fa\u4e86\u91cd\u8981\u7684\u76ee\u6a19\u3002BioVision\u7684SARS-CoV-2 S1\u86cb\u767d-ACE2\u7d50\u5408\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2(SARS-CoV-2 S1 Protein-ACE2 Binding Inhibitor Screening Kit)\u53ef\u7528\u65bc\u7be9\u9078S1\u86cb\u767d\u8207\u4ebaACE2\u7d50\u5408\u7684\u6f5b\u5728\u6291\u5236\u5291\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u4f7f\u7528\u93c8\u9ef4\u89aa\u548c\u7d20-HRP\u6aa2\u6e2cS1\u86cb\u767d\u8207\u751f\u7269\u7d20\u5316\u7684\u4ebaACE2\u7684\u7d50\u5408\u3002\u96a8\u5f8c\uff0c\u6dfb\u52a0TMB\u5e95\u7269\u4ee5\u53ef\u8996\u5316HRP\u9176\u4fc3\u53cd\u61c9\uff0c\u5f9e\u800c\u751f\u6210\u85cd\u8272\u7522\u7269\uff0c\u4e00\u65e6\u6dfb\u52a0\u7d42\u6b62\u6eb6\u6db2\uff0c\u8a72\u7522\u7269\u5c07\u8b8a\u70ba\u9ec3\u8272\u3002\u9ec3\u8272\u7684\u5bc6\u5ea6\u8207S1\u86cb\u767d\u8207\u4ebaACE2\u7684\u7d50\u5408\u6210\u6b63\u6bd4\u3002\u7136\u800c\uff0c\u5728\u6709\u6548\u6291\u5236\u5291\u7684\u5b58\u5728\u4e0b\uff0cS1\u86cb\u767d\u8207\u4ebaACE2\u7684\u7d50\u5408\u88ab\u6291\u5236\uff0c\u5f9e\u800c\u9632\u6b62\u4e86\u984f\u8272\u7684\u7522\u751f\u3002\u8a72\u6e2c\u5b9a\u8a66\u5291\u76d2\u9069\u7528\u65bc96\u5b54\u5f62\u5f0f\uff0c\u53ef\u70ba\u9ad8\u901a\u91cf\u7be9\u9078S1\u86cb\u767d\u8207\u4ebaACE2\u7d50\u5408\u7684\u6f5b\u5728\u6291\u88fd\u5291\u63d0\u4f9b\u53ef\u9760\u7684\u6e2c\u8a66\u65b9\u6cd5\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a><\/span> (<\/u><\/strong>405\u3001450\u3001492\u3001630 nm) \u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK2050-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nBioVision\u2019s SARS-CoV-2 S1 Protein-ACE2 Binding Inhibitor Screening Kit can be used to screen for potential inhibitors of S1 protein binding to human ACE2. In this assay, the binding of S1 protein to biotinylated human ACE2 is detected using Streptavidin-HRP. Subsequently, a TMB substrate is added to visualize the HRP enzymatic reaction thereby generating a blue colored product that changes to yellow once the stop solution is added. The density of the yellow color is proportional to the binding of S1 protein to Human ACE2. However, in the presence of potent inhibitor(s), the binding of S1 protein to Human ACE2 is suppressed thereby preventing the color generation. The assay kit is adapted to a 96-well format and provides a reliable test for high throughput screening of potential inhibitors of S1 protein binding to Human ACE2.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance at 450 nm<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Screening or characterizing inhibitors of SARS-CoV-2, S1 protein binding to human ACE2<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple and easy to use
\n\u2022 Provides a reliable test for high throughput screening of potential inhibitors of S1 protein binding to Human ACE2
\n\u2022 The assay kit is adapted to a 96-well format<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 S1 Protein coated Microplate
\n\u2022 Biotinylated Human ACE2
\n\u2022 Streptavidin-HRP
\n\u2022 SARS-CoV-2 Inhibitor
\n\u2022 TMB Substrate
\n\u2022 Stop Solution
\n\u2022 Wash Buffer (10X)
\n\u2022 Assay Diluent
\n\u2022 Plate Sealers<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00baC<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

SARS-CoV-2 S1\u86cb\u767d-ACE2\u7d50\u5408\u6291\u5236\u5291\u7be9\u9078\u8a66\u5291\u76d2 | \u8ca8\u865fK2050-100 \u00a0\u7522\u54c1\u63cf\u8ff0: \u56b4\u91cd\u6025 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1217],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26841"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26841"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26841\/revisions"}],"predecessor-version":[{"id":26844,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26841\/revisions\/26844"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26841"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26841"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26841"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}