{"id":2677,"date":"2014-05-28T17:23:46","date_gmt":"2014-05-28T09:23:46","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=2677"},"modified":"2022-07-27T17:12:08","modified_gmt":"2022-07-27T09:12:08","slug":"bioluminescence%e5%86%b7%e5%85%89%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e5%a5%97%e7%b5%84%ef%bc%8dbiothema","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=2677","title":{"rendered":"\u3010\u751f\u7269\u51b7\u5149\u3011Bioluminescence\u51b7\u5149\u5206\u6790\u8a66\u5291\u5957\u7d44\uff0dBiothema"},"content":{"rendered":"<p style=\"text-align: justify;\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-2692\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header.jpg\" alt=\"biothema_header\" width=\"676\" height=\"100\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header.jpg 676w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header-300x44.jpg 300w\" sizes=\"(max-width: 676px) 100vw, 676px\" \/><span style=\"color: #666699;\">\u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc\u745e\u5178\uff0c\u5c08\u9580\u7814\u767c\u751f\u7522\u51b7\u5149\uff08Bioluminescence\uff09\u4e4b\u76f8\u95dc\u7522\u54c1\uff0c\u63d0\u4f9b\u9ad8\u54c1\u8cea\u3001\u9ad8\u7a69\u5b9a\u6027\u4e4bATP\u8207\u51b7\u5149\u5206\u6790\u8a66\u5291\u5957\u7d44\u3002\u5176\u61c9\u7528\u5305\u62ec\u5feb\u901f\u7d30\u80de\u8a08\u6578\u3001\u7d30\u80de\u589e\u751f\u8207\u6bd2\u6027\u3001\u514d\u75ab\u8a66\u9a57\u3001\u9175\u7d20\u8207\u4ee3\u8b1d\u7269\u76e3\u63a7\u3001Luciferase reporter geneassay\u3001\u74b0\u5883\u885b\u751f\u6aa2\u6e2c\u7b49\u5206\u6790\u3002<\/span><\/p>\n<div id=\"attachment_2678\" style=\"width: 410px\" class=\"wp-caption aligncenter\"><img aria-describedby=\"caption-attachment-2678\" decoding=\"async\" loading=\"lazy\" class=\"wp-image-2678 size-full\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biothema_classification.jpg\" alt=\"Biothema \u7522\u54c1\u61c9\u7528\u5206\u985e\" width=\"400\" height=\"330\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biothema_classification.jpg 400w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biothema_classification-300x247.jpg 300w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><p id=\"caption-attachment-2678\" class=\"wp-caption-text\">Biothema \u7522\u54c1\u61c9\u7528\u5206\u985e<\/p><\/div>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u898f\u683c<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"100\"><span style=\"color: #008000;\"><strong>ATP\u751f\u6210\u4ee3\u8b1d\u00a0\u00a0<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">111-021<\/td>\n<td width=\"476\">Kinase RR Kit\uff0d\u5075\u6e2cKinase\u6216aminoacyl tRNA\u5408\u6210\u9176\u53ca\u76f8\u95dc\u53d7\u8cea<\/td>\n<td style=\"text-align: center;\" width=\"100\">1000 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">144-041<\/td>\n<td width=\"253\">ATP Kit SL\uff0d\u5075\u6e2cATP\u751f\u6210\u8207\u964d\u89e3<\/td>\n<td style=\"text-align: center;\" width=\"83\">1000 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"80\"><span style=\"color: #008000;\"><strong>\u5feb\u901f\u7d30\u80de\u8a08\u6578\u8207\u7d30\u80de\u6bd2\u6027\u5206\u6790<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">155-050<\/td>\n<td width=\"253\">Cellular ATP Kit HTS\uff0d\u5075\u6e2c<span style=\"color: #ff9900;\">Mammalian cells<\/span>\u4e14\u4f7f\u7528\u5177\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td style=\"text-align: center;\" width=\"83\">1200 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">188-441<\/td>\n<td width=\"253\">Cell Viability Kit SL\uff0d\u5075\u6e2c<span style=\"color: #ff9900;\">Mammalian cells<\/span>\u4e14\u4f7f\u7528\u7121\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td style=\"text-align: center;\" width=\"83\">1600 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"80\"><a title=\"Biothema \u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf\u5957\u7d44\" href=\"http:\/\/biopioneer.com.tw\/?p=153\"><span style=\"color: #008000;\"><strong>\u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf<\/strong><\/span><\/a><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">266-111<\/td>\n<td width=\"253\">Intracellular ATP Kit HS\uff0d\u5075\u6e2c\u542b\u6709Mammalian cells\u53ca\u542b\u6709\u7d30\u80de\u5916ATP\u4e4b<span style=\"color: #3366ff;\">\u5fae\u751f\u7269<\/span><\/td>\n<td style=\"text-align: center;\" width=\"83\">375 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">266-112<\/td>\n<td width=\"253\">Microbial ATP Kit HS\uff0d\u5075\u6e2c\u7121Mammalian cells\u4f46\u542b\u6709\u7d30\u80de\u5916ATP\u4e4b<span style=\"color: #3366ff;\">\u5fae\u751f\u7269<\/span><\/td>\n<td style=\"text-align: center;\" width=\"83\">375 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">266-311<\/td>\n<td width=\"253\">ATP Biomass Kit HS\uff0d\u5075\u6e2c\u7121Mammalian cells\u53ca\u7d30\u80de\u5916ATP\u4e4b<span style=\"color: #3366ff;\">\u5fae\u751f\u7269<\/span><\/td>\n<td style=\"text-align: center;\" width=\"83\">375 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"80\"><span style=\"color: #008000;\"><strong>Luciferase reporter gene assay<\/strong><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">484-001<\/td>\n<td width=\"253\">Luciferase Assay Kit<\/td>\n<td style=\"text-align: center;\" width=\"83\">800 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">484-102<\/td>\n<td width=\"253\">Luciferase Assay Kit HTS\uff0d\u9069\u5408\u5927\u91cf\u5206\u6790\u4e14\u7121\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td style=\"text-align: center;\" width=\"83\">4800 tests<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"80\">484-103<\/td>\n<td width=\"253\">Luciferase Assay Kit HTS\uff0d\u9069\u5408\u5927\u91cf\u5206\u6790\u4e14\u5177\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td style=\"text-align: center;\" width=\"83\">4600 tests<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=15166\"><strong><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E7%9B%B8%E9%97%9C%E8%A9%A6%E5%8A%91-300x144.png\" alt=\"\u76f8\u95dc\u8a66\u5291\" width=\"109\" height=\"52\" \/>\u00a0<span style=\"color: #008080;\">\u7d30\u80de\u6d3b\u529b\u8a66\u5291\u76d2<\/span><\/strong><\/a><span style=\"color: #008080;\">\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=15246\">ATP\u5f62\u6210\u8207\u964d\u89e3\u6e2c\u91cf<\/a>\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=6698\"><strong style=\"color: #00ccff;\">\u5fae\u751f\u7269<\/strong><strong style=\"color: #00ccff;\">ATP<\/strong><strong style=\"color: #00ccff;\">\u6aa2\u6e2c<\/strong><strong style=\"color: #00ccff;\">\u5957\u7d44<\/strong><\/a>\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-2692\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header.jpg\" alt=\"biothema_header\" width=\"676\" height=\"100\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header.jpg 676w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header-300x44.jpg 300w\" sizes=\"(max-width: 676px) 100vw, 676px\" \/><\/p>\n<p style=\"text-align: justify;\"><span style=\"color: #666699;\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%93%81%E7%89%8C%E4%BB%8B%E7%B4%B9-300x144.png\" alt=\"\u54c1\u724c\u4ecb\u7d39\" width=\"111\" height=\"53\" \/>\u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc\u745e\u5178\uff0c\u5c08\u9580\u7814\u767c\u751f\u7522\u51b7\u5149\uff08Bioluminescence\uff09\u4e4b\u76f8\u95dc\u7522\u54c1\uff0c\u63d0\u4f9b\u9ad8\u54c1\u8cea\u3001\u9ad8\u7a69\u5b9a\u6027\u4e4bATP\u8207\u51b7\u5149\u5206\u6790\u8a66\u5291\u5957\u7d44\u3002\u5176\u61c9\u7528\u5305\u62ec\u5feb\u901f\u7d30\u80de\u8a08\u6578\u3001\u7d30\u80de\u589e\u751f\u8207\u6bd2\u6027\u3001\u514d\u75ab\u8a66\u9a57\u3001\u9175\u7d20\u8207\u4ee3\u8b1d\u7269\u76e3\u63a7\u3001Luciferase reporter geneassay\u3001\u74b0\u5883\u885b\u751f\u6aa2\u6e2c\u7b49\u5206\u6790\u3002<\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000ATP\u751f\u7269\u51b7\u5149\u53cd\u61c9\u6280\u8853\u662f\u4e00\u7a2e\u5feb\u901f\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u7528\u4ee5\u6aa2\u6e2c\u751f\u7269ATP\u7684\u542b\u91cf\u3002\u6b64\u65b9\u6cd5\u70ba\u7f8e\u570b\u592a\u7a7a\u7e3d\u7f72\u65bc1960\u5e74\u4ee3\u767c\u5c55\u51fa\u4f86\u7684\u6280\u8853\uff0c\u7576\u521d\u767c\u5c55\u6b64\u9805\u6280\u8853\u7684\u76ee\u7684\u662f\u5e0c\u671b\u80fd\u7528\u4ee5\u6aa2\u6e2c\u5916\u592a\u7a7a\u662f\u5426\u6709\u751f\u547d\u7269\u8cea\u5b58\u5728\uff0c\u5176\u53cd\u61c9\u539f\u7406\u8207\u87a2\u706b\u87f2\u9ad4\u5167\u767c\u5149\u53cd\u61c9\u76f8\u540c\uff0c\u5728\u87a2\u5149\u7d20\uff08luciferin\uff09\u8207\u87a2\u5149\u9175\u7d20\uff08luciferase\uff09\u5b58\u5728\u4e0b\uff0cATP\u8207luciferin\u53caluciferase\u50ac\u5316\u4e0b\u7522\u751f\u6de1\u85cd\u8272\u7684\u5149\u3002\u53cd\u61c9\u65b9\u7a0b\u5f0f\u5982\u4e0b\uff1a<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-607 aligncenter\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP_formula.png\" alt=\"rapid_ATP_formula\" width=\"500\" height=\"33\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP_formula.png 500w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP_formula-300x19.png 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u8fd1\u4f86\u751f\u7269ATP\u51b7\u5149\u53cd\u61c9\u5728\u98df\u54c1\u8207\u91ab\u85e5\u9818\u57df\u4e2d\u88ab\u5ee3\u6cdb\u4f7f\u7528\u3002\u4ee5\u539f\u6838\u751f\u7269\u800c\u8a00\uff0c\u4e00\u96bb\u7d30\u83cc\u9ad4\u5167\u7d04\u542b2 x 10<sup>-18<\/sup>\u00a0mol ATP\uff1b\u771f\u83cc\u7d30\u80de\u5247\u542b10<sup>-15<\/sup>\uff5e10<sup>-16<\/sup>\u00a0mol ATP\u3002\u61c9\u7528ATP\u51b7\u5149\u53cd\u61c9\u6280\u8853\u6aa2\u6e2c\u751f\u83cc\u6578\u6642\uff0c\u9700\u5148\u5c07\u98df\u54c1\u4e2d\u975e\u5fae\u751f\u7269\u7684ATP\u6c34\u89e3\uff0c\u518d\u5c07\u5fae\u751f\u7269\u9ad4\u5167\u7684ATP\u91cb\u653e\u51fa\u4f86\uff0c\u53cd\u61c9\u5f8c\u4ee5ATP\u751f\u7269\u51b7\u5149\u5100\u5075\u6e2c\u3002\u82e5\u6aa2\u6e2c\u98df\u54c1\u63a5\u89f8\u8868\u9762\u7684\u6e05\u6f54\u5ea6\uff0c\u5247\u7121\u9700\u53bb\u9664\u98df\u54c1\u4e2d\u7684ATP\uff0c\u76f4\u63a5\u6e2c\u91cf\u5373\u53ef\u3002\u6aa2\u6e2c\u7d50\u679c\u5247\u4ee5\u76f8\u5c0d\u5438\u5149\u503c\uff08relative light unit, RLU\uff09\u7684\u591a\u5be1\u4f86\u8868\u793aATP\u6fc3\u5ea6\u7684\u9ad8\u4f4e\uff0c\u85c9\u6b64\u53cd\u63a8\u5fae\u751f\u7269\u542b\u91cf\u548c\u53ef\u80fd\u7684\u98df\u7269\u6b98\u7559\uff0c\u4ee5\u8868\u793a\u98df\u7269\u63a5\u89f8\u9762\u6e05\u6f54\u5ea6\u7684\u60c5\u6cc1\u3002<\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u76ee\u524dATP\u751f\u7269\u51b7\u5149\u53cd\u61c9\u6280\u8853\u61c9\u7528\u5728\u5404\u9805\u98df\u54c1\u7522\u696d\uff0c\u5305\u62ec\u751f\u4e73\u751f\u83cc\u6578\u4f30\u7b97\u3001\u74b0\u5883\u8a2d\u5099\u6e05\u6f54\u8a55\u4f30\u3001\u755c\u7522\u696d\u4e2d\u5c60\u9ad4\u7684\u6c61\u67d3\u8a55\u4f30\u3001\u679c\u6c41\u7522\u696d\u7684\u751f\u83cc\u6578\u4f30\u7b97\u3001\u88fd\u9152\u5de5\u696d\u53ca\u98f2\u6599\u696d\u7b49\u3002<\/p>\n<p><span style=\"font-size: medium; color: #008000;\">\u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf\u5957\u7d44\u7522\u54c1\u5206\u985e\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/biothema.se\/wp-content\/uploads\/2011\/05\/Product-selection-guide-WEB-2012.pdf\" target=\"_blank\"><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-606 aligncenter\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP.png\" alt=\"rapid_atp_chart\" width=\"671\" height=\"266\" srcset=\"https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP.png 671w, https:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP-300x118.png 300w\" sizes=\"(max-width: 671px) 100vw, 671px\" \/><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\">\n<tbody>\n<tr>\n<td width=\"100\">\n<p align=\"center\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n<td width=\"476\">\n<p align=\"center\"><strong>\u54c1\u540d<\/strong><\/p>\n<\/td>\n<td width=\"100\">\n<p align=\"center\"><strong>\u898f\u683c<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">\n<p align=\"center\">266-112<\/p>\n<\/td>\n<td width=\"476\">Microbial ATP Kit HS<\/td>\n<td nowrap=\"nowrap\" width=\"100\">\n<p align=\"center\">375 test<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">\n<p align=\"center\">266-111<\/p>\n<\/td>\n<td width=\"476\">Intracellular ATP Kit HS<\/td>\n<td width=\"100\">\n<p align=\"center\">375 test<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">\n<p align=\"center\">266-311<\/p>\n<\/td>\n<td valign=\"center\" width=\"476\">ATP Biomass Kit HS<\/td>\n<td width=\"100\">\n<p align=\"center\">375 test<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc\u745e\u5178\uff0c\u5c08\u9580\u7814\u767c\u751f\u7522\u51b7\u5149\uff08Bioluminescence\uff09\u4e4b\u76f8\u95dc\u7522\u54c1\uff0c\u63d0\u4f9b [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2680,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[133,123,3],"tags":[4797],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/2677"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2677"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/2677\/revisions"}],"predecessor-version":[{"id":31769,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/2677\/revisions\/31769"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2680"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2677"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2677"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2677"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}