{"id":26507,"date":"2020-12-07T11:13:10","date_gmt":"2020-12-07T03:13:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26507"},"modified":"2022-07-19T15:43:49","modified_gmt":"2022-07-19T07:43:49","slug":"sars-cov-2-rbd-%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fe4877","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=26507","title":{"rendered":"SARS-CoV-2 RBD \u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fE4877"},"content":{"rendered":"

SARS-CoV-2 RBD \u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865f<\/strong>E4877<\/a><\/span><\/p>\n

SARS-CoV-2 RBD ELISA Kit<\/p>\n

\u7522\u54c1\u4ecb\u7d39: COVID-19\u75c5\u662f\u4e00\u7a2e\u7531\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2(novel coronavirus)\u5f15\u8d77\u7684\u547c\u5438\u7cfb\u7d71\u75be\u75c5(respiratory illness)\uff0c\u9019\u7a2e\u51a0\u72c0\u75c5\u6bd2\u88ab\u7a31\u70ba\u7cfb\u7d71\u6027\u6025\u6027\u547c\u5438\u7cfb\u7d71\u7d9c\u5408\u75c7\u51a0\u72c0\u75c5\u6bd22\uff08novel coronavirus called systemic acute respiratory syndrome coronavirus-2, SARS-CoV-2\uff09\u3002SARS-CoV-2\u5c6c\u65bc\u03b2\u51a0\u72c0\u75c5\u6bd2\u5bb6\u65cf(\u03b2-coronavirus)\u3002\u5b83\u7684\u57fa\u56e0\u7d44(genome)\u9577\u7d0430 kb\uff0c\u8207SARS-CoV\u5171\u4eab\u7d0480\uff05\u7684\u57fa\u56e0\u7d44\u540c\u4e00\u6027\u3002 3’\u672b\u7aef(3\u2019 proximal terminal)\u75314\u7a2e\u7d50\u69cb\u86cb\u767d\u7d44\u6210\uff1aSpike\uff08Spike , S\uff09\uff0cMembrane\uff08Membrane , M\uff09\uff0cEnvelope\uff08Envelope , E\uff09\u548c\u6838\u4ec1\uff08Nucleocapsid , N\uff09\u3002S\u86cb\u767d(S protein)\u662f\u540c\u578b\u4e09\u805a\u9ad4(homo-trimeric)\uff0cI\u985e\u878d\u5408\u8de8\u819c\u7cd6\u86cb\u767d(class I fusion, transmembrane glycoprotein)\u3002\u5b83\u4fc3\u9032\u75c5\u6bd2\u7684\u9644\u8457(attachment)\uff0c\u878d\u5408(fusion)\u548c\u9032\u5165\u5bbf\u4e3b\u7d30\u80de(entry of the virus)\u3002\u8a72\u86cb\u767d\u8cea\u7531\u5169\u500b\u529f\u80fd\u4e9e\u57faS1\u548cS2\u7d44\u6210\u3002 S1\u4e9e\u57fa(subunit)\u5305\u542bN\u7aef\u7d50\u69cb\u57df\uff08N-terminal domain , NTD\uff09\u548cC\u7aef\u7d50\u69cb\u57df\uff08C-terminal domain , CTD\uff09\u3002 C\u672b\u7aef\u7d50\u69cb\u57df\uff08C-terminal domain , CTD\uff09\u7531\u53d7\u9ad4\u7d50\u5408\u7d50\u69cb\u57df\uff08receptor-binding domain , RBD\uff09\u7d44\u6210\uff0c\u8a72\u53d7\u9ad4\u7d50\u5408\u7d50\u69cb\u57df\u8207\u4f4d\u65bc\u5bbf\u4e3b\u7d30\u80de\u4e0a\u7684\u8840\u7ba1\u7dca\u5f35\u7d20\u8f49\u5316\u91762\uff08angiotensin-converting enzyme 2,ACE2\uff09\u53d7\u9ad4\u7d50\u5408\u3002\u8003\u616e\u5230RBD\u5728\u75c5\u6bd2\u9032\u5165\u5bbf\u4e3b\u7d30\u80de\u4e2d\u7684\u95dc\u9375\u4f5c\u7528\uff0c\u5b83\u53ef\u4ee5\u4f5c\u70ba\u958b\u767c\u75ab\u82d7\u7684\u6709\u6548\u9776\u6297\u539f\u3002BioVision\u7684SARS-CoV-2 RBD ELISA\u8a66\u5291\u76d2\u65e8\u5728\u5b9a\u91cf\u6e2c\u91cf\u652f\u6c23\u7ba1\u80ba\u6ce1\u704c\u6d17\u6db2(bronchoalveolar lavage fluid)\u548c\u9f3b\u54bd\u62ed\u5b50\u6a23\u54c1(nasopharyngeal swab samples)\u4e2d\u7684RBD\u86cb\u767d\u542b\u91cf\u3002\u8a72\u6e2c\u5b9a\u57fa\u65bcSandwich ELISA principle\u539f\u7406\u3002\u5c07\u6e2c\u8a66\u6a23\u54c1\uff0c\u6a19\u6e96\u54c1\u548c\u751f\u7269\u7d20\u7db4\u5408\u7684\u6297\u9ad4\u6dfb\u52a0\u5230\u9810\u5148\u5857\u6709RBD\u6297\u9ad4\u7684\u5b54\u4e2d\uff0c\u7136\u5f8c\u7528\u6d17\u6ecc\u7de9\u885d\u6db2\u6d17\u6ecc\u3002\u52a0\u5165HRP-\u93c8\u9ef4\u89aa\u548c\u7d20\uff0c\u4e26\u4f7f\u7528\u6d17\u6ecc\u7de9\u885d\u6db2\u5c07\u4efb\u4f55\u672a\u9023\u63a5\u7684\u7d50\u5408\u7269\u6d17\u6389\u3002\u901a\u904e\u6dfb\u52a0TMB\u5e95\u7269\u6aa2\u6e2cHRP\u9176\u4fc3\u53cd\u61c9\u3002\u6700\u5f8c\uff0c\u7528\u9178\u6027\u7d42\u6b62\u6eb6\u6db2(acidic stop solution)\u7d42\u6b62\u53cd\u61c9\u3002\u986f\u8272\u8207\u6a23\u54c1\u6216\u6a19\u6e96\u6db2\u4e2dRBD\u7684\u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002<\/p>\n

\"BioVision<\/p>\n

\u5546\u54c1\u9700\u7528\u5100\u5668\u6e2c\u91cf<\/span>\u00a0\u00a0\u9175\u7d20\u514d\u75ab\u5206\u6790\u5100\u8acb\u6309\u6b64\u00a0<\/a><\/span>(\u6a19\u914d\u6ffe\u7247405\u3001450\u3001492\u3001630 nm)<\/p>\n

\"RT-6900\"<\/a><\/p>\n

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\"rt-2600c\"<\/a><\/p>\n

\u82e5\u9700ELISA\u5fae\u91cf\u76e4\u6e05\u6d17\u6a5f\u8acb\u6309\u6b64<\/a><\/span><\/p>\n

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 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nE4877-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n96 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nSandwich ELISA kit to Quantitatively determine the concentration of RBD protein in bronchoalveolar lavage fluid and nasopharyngeal swab samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Sensitivity: < 10 pg\/ml
\n\u2022 Detection range: 12.5 \u2013 800 pg\/ml
\n\u2022 This Sandwich ELISA is highly sensitive and highly specific for the detection of RBD protein in bronchoalveolar lavage fluid and nasopharyngeal samples
\n\u2022 Assay Precision: Intra-Assay CV < 12% and Inter-Assay CV < 15%<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Microtiter ELISA plate
\n\u2022 SARS-CoV-2 RBD Standard (1600 pg)
\n\u2022 Standard\/Sample Dilution Buffer
\n\u2022 RBD Biotin Conjugated Antibody
\n\u2022 Reagent Dilution Buffer
\n\u2022 HRP-Streptavidin Conjugate (SABC)
\n\u2022 TMB Substrate Solution
\n\u2022 Stop Solution
\n\u2022 Wash Buffer (20X)
\n\u2022 Plate Sealer<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u6e2c\u91cf\u7269<\/td>\n\u6a23\u54c1\u4f86\u6e90<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865f<\/u><\/strong>E4903<\/a><\/td>\nSARS-CoV-2 Nucleocapsid IgG\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/a><\/td>\n\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u6838\u8863\u6bbc\u86cb\u767d(nucleocapsid protein)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372(capture)\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4\u3002<\/td>\nHuman Serum or Plasma (Heparin or EDTA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4902<\/a><\/td>\nSARS-CoV-2 IgM ELISA\u8a66\u5291\u76d2 <\/a><\/td>\n\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u523a\u7a81\u86cb\u767dS1\u4e9e\u57fa(spike protein S1 subunit)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4(anti-SARS-CoV-2 antibodies)\u3002<\/td>\nHuman Serum or Plasma (Heparin or EDTA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4877<\/a><\/td>\nSARS-CoV-2 RBD \u6aa2\u6e2c\u8a66\u5291\u76d2 <\/a><\/td>\n\u6e2c\u91cf\u652f\u6c23\u7ba1\u80ba\u6ce1\u704c\u6d17\u6db2(bronchoalveolar lavage fluid)\u548c\u9f3b\u54bd\u62ed\u5b50\u6a23\u54c1(nasopharyngeal swab samples)\u4e2d\u7684RBD\u86cb\u767d\u542b\u91cf\u3002<\/td>\nbronchoalveolar lavage fluid\u548c nasopharyngeal swab samples<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4901<\/a><\/td>\nSARS-CoV-2 IgG ELISA\u6aa2\u6e2c\u8a66\u5291 <\/a><\/td>\n\u8a72\u6e2c\u5b9a\u6cd5\u4f7f\u7528\u523a\u7a81\u86cb\u767dS1\u4e9e\u57fa(spike protein S1 subunit)\u4f5c\u70ba\u8a98\u990c\u4f86\u6355\u7372\u7a00\u91cb\u7684\u8840\u6f3f\u6216\u8840\u6e05\u6a23\u54c1\u4e2d\u7684\u6297SARS-CoV-2\u6297\u9ad4\u3002<\/td>\n\n

Human Serum or Plasma (Heparin or EDTA)<\/p>\n

 <\/p>\n

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 <\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>E4878<\/a><\/td>\nSARS-CoV-2 Nucleocapsid\u6aa2\u6e2c\u8a66\u5291\u76d2 <\/a><\/td>\n\u6e2c\u91cf\u652f\u6c23\u7ba1\u80ba\u6ce1\u704c\u6d17\u6db2(bronchoalveolar lavage fluid)\u548c\u9f3b\u54bd\u62ed\u5b50(nasopharyngeal swab samples)\u6a23\u54c1\u4e2dNucleocapsid\u86cb\u767d\u7684\u542b\u91cf\u3002<\/td>\nBronchoalveolar lavage fluid and nasopharyngeal samples<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>E4821-100<\/a><\/td>\nSARS-CoV-2\u6838\u86cb\u767dIgG\u6297\u9ad4ELISA\u8a66\u5291\u76d2 <\/a><\/td>\n\u6e2c\u91cfSARS-CoV-2\u6838\u86cb\u767dIgG\u6297\u9ad4<\/td>\nSerum, Plasma<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

SARS-CoV-2 RBD \u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fE4877 SARS-CoV-2 RBD ELISA Kit […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1287],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26507"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26507"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26507\/revisions"}],"predecessor-version":[{"id":26597,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26507\/revisions\/26597"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26507"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26507"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26507"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}