{"id":26235,"date":"2020-11-17T11:39:07","date_gmt":"2020-11-17T03:39:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26235"},"modified":"2022-07-19T16:09:55","modified_gmt":"2022-07-19T08:09:55","slug":"%e5%9f%ba%e5%9b%a0%e7%b5%84dna%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92-%e7%a3%81%e7%8f%a0-genomic-dna-extraction%e8%b2%a8%e8%99%9fk1442-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=26235","title":{"rendered":"\u57fa\u56e0\u7d44DNA\u63d0\u53d6\u8a66\u5291\u76d2 (\u78c1\u73e0) |genomic DNA extraction\u8ca8\u865fK1442-100"},"content":{"rendered":"

\u57fa\u56e0\u7d44DNA\u63d0\u53d6\u8a66\u5291\u76d2 (\u78c1\u73e0) | <\/strong>\u8ca8\u865f<\/strong>K1442-100<\/a>\u00a0\u7d30\u80de\/\u7d44\u7e54<\/span><\/p>\n

\u00a0<\/em><\/strong>\u7522\u54c1\u63cf\u8ff0:<\/strong> \u7d30\u80de(Cell)\u548c\u7d44\u7e54(tissue)\u57fa\u56e0\u7d44DNA\u63d0\u53d6(genomic DNA extraction)\u548c\u7d14\u5316\u8a66\u5291\u76d2\u9069\u7528\u65bc\u5f9e\u5404\u7a2e\u7d44\u7e54\u548c\u57f9\u990a\u7684\u7d30\u80de\u4e2d\u63d0\u53d6\u548c\u7d14\u5316\u57fa\u56e0\u7d44DNA(genomic DNA)\u3002\u5728\u7368\u7279\u7684\u7d44\u7e54\u7d30\u80de\u88c2\u89e3\u6eb6\u6db2(Tissue-Cell Lysis solution)\u4e2d\uff0c\u53ef\u4ee5\u6709\u6548\u5730\u63d0\u53d6\u57fa\u56e0\u7d44DNA\uff0c\u7136\u5f8c\u901a\u904e\u78c1\u73e0\u7d14\u5316(magnetic beads)\uff0c\u5f9e\u800c\u5f97\u5230\u9ad8\u7d14\u57fa\u56e0\u7d44DNA(genomic DNA)\uff0c\u5176OD260 \/ 280\u7684\u6bd4\u7387\u57281.75\u81f31.85\u4e4b\u9593\u3002\u56de\u6536\u7684\u57fa\u56e0\u7d44DNA(genomic DNA)\u5927\u5c0f\u53ef\u9ad8\u905460 kb\u3002\u7d14\u5316\u7684\u57fa\u56e0\u7d44DNA\u9069\u7528\u65bcPCR\u3001Southern\u5370\u8de1\u548c\u6e2c\u5e8f(sequencing)\u7b49\u61c9\u7528\u3002\u5982\u679c\u4f7f\u7528\u7279\u6b8a\u7684\u78c1\u6027\u6846\u67b6(magnetic frame)\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u820748\u5b54\u5713\u5f62\u5e95\u677f\u4e00\u8d77\u4f7f\u7528\u3002\u8a72\u8a66\u5291\u76d2\u9084\u53ef\u8207\u5404\u7a2e\u81ea\u52d5\u6838\u9178\u63d0\u53d6\u5100\u5668\u6216\u5de5\u4f5c\u7ad9\u4e00\u8d77\u4f7f\u7528\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK1442-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 tests<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\nGenomic DNA can be efficiently extracted and then purified by the magnetic beads, yielding high pure genomic DNA with a ratio of OD260 \/ 280 between 1.75 to 1.85.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nMagnetic beads<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nGenomic DNA can be efficiently extracted and then purified by the magnetic beads, yielding high pure genomic DNA with a ratio of OD260 \/ 280 between 1.75 to 1.85.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Easy and simple
\n\u2022 High pure genomic DNA can be efficiently extracted and then purified by the magnetic beads<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Elution Buffer
\n\u2022 Wash Solution
\n\u2022 Tissue-Cell Lysis solution
\n\u2022 Magnetic beads<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\nRT and 2-8\u00b0C as described in the datasheet<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nRT<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Tuantuan Tong, Theaflavins Improve Insulin Sensitivity through Regulating Mitochondrial Biosynthesis in Palmitic Acid-Induced HepG2 Cells.\u00a0Molecules, Dec 2018; \u00a030572687.<\/a><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u57fa\u56e0\u7d44DNA\u63d0\u53d6\u8a66\u5291\u76d2 (\u78c1\u73e0) | \u8ca8\u865fK1442-100\u00a0\u7d30\u80de\/\u7d44\u7e54 \u00a0\u7522\u54c1\u63cf\u8ff0: \u7d30\u80de(Cell)\u548c\u7d44\u7e54 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1338],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26235"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=26235"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26235\/revisions"}],"predecessor-version":[{"id":26245,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/26235\/revisions\/26245"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=26235"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=26235"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=26235"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}