{"id":26087,"date":"2020-11-05T11:16:43","date_gmt":"2020-11-05T03:16:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=26087"},"modified":"2022-07-19T16:14:49","modified_gmt":"2022-07-19T08:14:49","slug":"%e8%9e%a2%e5%85%89%e7%b4%a0%e9%85%b6%e5%a0%b1%e5%91%8a%e5%9f%ba%e5%9b%a0%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-luciferase-reporter-assay-kit%e8%b2%a8%e8%99%9fk801-200","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=26087","title":{"rendered":"\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u8a66\u5291\u76d2 | Luciferase Reporter Assay Kit\u8ca8\u865fK801-200"},"content":{"rendered":"

\u87a2\u5149\u7d20\u9176\u5831\u544a\u57fa\u56e0\u6aa2\u6e2c\u8a66\u5291\u76d2 | Luciferase Reporter Assay Kit<\/strong>\u8ca8\u865f<\/strong>K801-200<\/a><\/span><\/p>\n

\u00a0<\/em><\/strong>\u7522\u54c1\u63cf\u8ff0:<\/strong> \u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Firefly luciferase) \u5df2\u88ab\u7528\u4f5c\u7814\u7a76\u57fa\u56e0\u8abf\u63a7(gene regulation)\u548c\u529f\u80fd(function)\u7684\u654f\u611f\u5831\u544a\u57fa\u56e0\u3002\u8207\u6a19\u6e96\u7684\u6c2f\u9ef4\u7d20\u4e59\u9170\u8f49\u79fb\u9176\uff08chloramphenicol acetyltransferase , CAT\uff09\u5206\u6790\u76f8\u6bd4\uff0c\u87a2\u5149\u7d20\u9176\u7684\u9748\u654f\u5ea6\u63d0\u9ad8\u4e861000\u500d\u3002\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Firefly luciferase)\u50ac\u5316\u87a2\u5149\u7d20\u7684\u6c27\u5316\u7fa7\u5316(oxidative carboxylation)\u53cd\u61c9\uff0c\u662f\u4efb\u4f55\u5df2\u77e5\u751f\u7269\u767c\u5149\u53cd\u61c9\u4e2d\u6548\u7387\u6700\u9ad8\u7684\u53cd\u61c9\u3002\u53cd\u61c9\u7684\u5149\u767c\u5c04\u53ef\u4ee5\u4f7f\u7528luminometer\u8a18\u9304\u3002 BioVision\u7684\u87a2\u5149\u7d20\u9176\u5831\u544a\u8a66\u5291\u76d2(Luciferase Reporter Assay Kit, K801)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u6aa2\u6e2c\u8f49\u67d3\u7684\u771f\u6838\u7d30\u80de\u4e2d\u7684\u87a2\u5149\u7d20\u9176\u6d3b\u6027\u3002\u70ba\u4e86\u5728\u9ad4\u5916\u6e2c\u91cf\u8868\u9054\u7684\u87a2\u5149\u7d20\u9176\uff0c\u9996\u5148\u901a\u904e\u7d30\u80de\u88c2\u89e3\u5f9e\u8f49\u67d3\u7684\u7d30\u80de\u4e2d\u63d0\u53d6\u87a2\u5149\u7d20\u9176\u3002\u7136\u5f8c\u5c07CoA\uff0cATP\uff0cMg 2+\u548c\u7de9\u885d\u6db2\u6dfb\u52a0\u5230\u88c2\u89e3\u7269\u4e2d\uff08\u5305\u542bCoA\u6703\u7522\u751f\u5e7e\u4e4e\u6046\u5b9a\u7684\u767c\u5149\uff0c\u800c\u4e0d\u662f\u5178\u578b\u7684\u9583\u5149\u52d5\u529b\u5b78\uff09\u3002\u7136\u5f8c\u901a\u904e\u6ce8\u5165\u87a2\u5149\u7d20\u89f8\u767c\u767c\u5149\u53cd\u61c9\uff0c\u4e26\u901a\u904eluminometer\u8a18\u9304\u767c\u5c04\u7684\u5149\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK801-200<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n200 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nLuminescence<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis Luciferase Reporter Assay Kit provides a simple means for detecting luciferase activity in transfected eukaryotic cells.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 The assay ensures maximal sensitivity, consistent light output, as well as accuracy and consistency when working with multiple samples.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Substrate A
\n\u2022 Substrate B
\n\u2022 Cell Lysis Buffer<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA\uff09\u6e2c\u5b9a<\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/a>\u00a0|\u00a0 <\/strong>Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA\u5100\u5668<\/a><\/span><\/p>\n

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\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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