{"id":25878,"date":"2020-09-02T17:57:39","date_gmt":"2020-09-02T09:57:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25878"},"modified":"2022-07-19T16:44:45","modified_gmt":"2022-07-19T08:44:45","slug":"aldh2-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-human-aldehyde-dehydrogenase-aldh-elisa-kit%e8%b2%a8%e8%99%9fkte62505","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=25878","title":{"rendered":"ALDH2 ELISA\u8a66\u5291\u76d2| Human Aldehyde dehydrogenase (ALDH) ELISA Kit\u8ca8\u865fKTE62505"},"content":{"rendered":"<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=25878\">ALDH2 ELISA\u8a66\u5291\u76d2<strong>| Human Aldehyde dehydrogenase (ALDH) ELISA Kit<\/strong><strong>\u8ca8\u865f<\/strong>KTE62505<\/a><\/span><\/p>\n<p>ALDH2\u5c6c\u65bc\u86cb\u767d\u8cea\u7684\u919b\u812b\u6c2b\u9176\u5bb6\u65cf(aldehyde dehydrogenase family)\u3002\u919b\u812b\u6c2b\u9176(Aldehyde dehydrogenase)\u662f\u9152\u7cbe\u4ee3\u8b1d\u7684\u4e3b\u8981\u6c27\u5316\u9014\u5f91(alcohol metabolism)\u4e2d\u7684\u7b2c\u4e8c\u7a2e\u9176\u3002\u919b\u812b\u6c2b\u9176(aldehyde dehydrogenase)\u7684\u5169\u7a2e\u4e3b\u8981\u809d\u81df\u540c\u5de5\u578b(liver isoforms)\uff0c\u80de\u8cea(cytosolic)\u548c\u7dda\u7c92\u9ad4(mitochondrial)\uff0c\u53ef\u4ee5\u901a\u904e\u5176\u96fb\u6cf3\u9077\u79fb\u7387(electrophoretic mobilities)\uff0c\u52d5\u529b\u5b78(kinetic properties)\u7279\u6027\u548c\u4e9e\u7d30\u80de\u5b9a\u4f4d(subcellular localizations)\u4f86\u5340\u5206\u3002\u5927\u591a\u6578\u9ad8\u52a0\u7d22(Caucasians)\u4eba\u6709\u5169\u7a2e\u4e3b\u8981\u7684\u540c\u5de5\u9176\uff0c\u800c\u5927\u7d0450\uff05\u7684\u6771\u4e9e\u4eba(East Asians)\u5177\u6709\u80de\u6f3f\u540c\u5de5\u9176(cytosolic isozyme)\uff0c\u4f46\u6c92\u6709\u7dda\u7c92\u9ad4\u540c\u5de5\u9176(mitochondrial isozyme)\u3002\u6771\u4e9e\u4eba\u4e2d\u6025\u6027\u9152\u7cbe\u4e2d\u6bd2\u7684\u983b\u7387\u6bd4\u767d\u7a2e\u4eba\u4e2d\u9ad8\u5f97\u591a\uff0c\u9019\u53ef\u80fd\u8207\u7dda\u7c92\u9ad4\u540c\u5de5\u9176(mitochondrial isozyme)\u7f3a\u4e4f\u50ac\u5316\u6d3b\u6027\u5f62\u5f0f\u6709\u95dc\u3002\u5177\u6709\u50ac\u5316\u5931\u6d3b\u5f62\u5f0f\u7684\u500b\u9ad4\u5c0d\u4e59\u919b\u7684\u66b4\u9732\u589e\u52a0\u4e5f\u53ef\u80fd\u8ce6\u4e88\u8a31\u591a\u985e\u578b\u7684\u764c\u75c7\u66f4\u5927\u7684\u654f\u611f\u6027\u3002\u8a72\u57fa\u56e0\u7de8\u78bc\u7dda\u7c92\u9ad4\u540c\u5de5\u578b(mitochondrial isoform)\uff0c\u5176\u5c0d\u65bc\u4e59\u919b\u7684Km\u4f4e\uff0c\u4f4d\u65bc\u7dda\u7c92\u9ad4\u57fa\u8cea(mitochondrial matrix)\u4e2d\u3002\u9078\u64c7\u6027\u526a\u63a5\u5c0e\u81f4\u7de8\u78bc\u4e0d\u540c\u540c\u5de5\u578b\u7684\u591a\u500b\u8f49\u9304\u8b8a\u9ad4\u3002<\/p>\n<p><strong>\u7522\u54c1<\/strong><strong>\u63cf\u8ff0\uff1a\u4eba<\/strong>\u919b\u812b\u6c2b\u9176\uff08Human Aldehyde dehydrogenase , ALDH\uff09ELISA\u8a66\u5291\u76d2\u63a1\u7528\u5169\u9ede\u593e\u5fc3ELISA(Sandwich ELISA)\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684ALDH2\u3002\u5c0dALDH2\u7279\u7570\u7684\u6297\u9ad4\u5df2\u9810\u5148\u5305\u88ab\u5728\u5fae\u5b54\u677f\u4e0a\u3002\u5c07\u6a19\u6e96\u54c1\u548c\u6a23\u54c1\u5438\u53d6\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u5b58\u5728\u7684\u4efb\u4f55ALDH2\u90fd\u88ab\u56fa\u5b9a\u7684\u6297\u9ad4\u7d50\u5408\u3002\u9664\u53bb\u6240\u6709\u672a\u7d50\u5408\u7684\u7269\u8cea\u5f8c\uff0c\u5c07HRP-\u7db4\u5408ALDH2\u6aa2\u6e2c\u6297\u9ad4\u6dfb\u52a0\u5230\u5b54\u4e2d\u3002\u6d17\u6ecc\u4ee5\u9664\u53bb\u4efb\u4f55\u672a\u7d50\u5408\u7684HRP\u8a66\u5291\u5f8c\uff0c\u5c07\u8272\u539f\u6eb6\u6db2\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u6839\u64da\u5728\u521d\u59cb\u6b65\u9a5f\u4e2d\u7d50\u5408\u7684ALDH2\u7684\u91cf\u6210\u6bd4\u4f8b\u5730\u986f\u8272\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n<ul>\n<li><strong>Product name<\/strong><\/li>\n<\/ul>\n<p>Human Aldehyde dehydrogenase (ALDH) ELISA Kit<\/p>\n<ul>\n<li><strong>Reactivity<\/strong><\/li>\n<\/ul>\n<p>Human<\/p>\n<ul>\n<li><strong>Applications<\/strong><\/li>\n<\/ul>\n<p>ELISA<\/p>\n<ul>\n<li><strong>Detection method<\/strong><\/li>\n<\/ul>\n<p>Colorimetric<\/p>\n<ul>\n<li><strong>Sample type<\/strong><\/li>\n<\/ul>\n<p>Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n<ul>\n<li><strong>Assay type<\/strong><\/li>\n<\/ul>\n<p>Sandwich ELISA (quantitative)<\/p>\n<ul>\n<li><strong>Assay duration<\/strong><\/li>\n<\/ul>\n<p>Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.<\/p>\n<ul>\n<li><strong>Kit components<\/strong><\/li>\n<li>Human Aldehyde dehydrogenase microplate<br \/>\n\u2022 Human Aldehyde dehydrogenase standard<br \/>\n\u2022 Human Aldehyde dehydrogenase detect antibody<br \/>\n\u2022 Streptavidin-HRP<br \/>\n\u2022 Standard diluent<br \/>\n\u2022 Assay buffer<br \/>\n\u2022 HRP substrate<br \/>\n\u2022 Stop solution<br \/>\n\u2022 Wash buffer<br \/>\n\u2022 Plate covers<\/li>\n<li><strong>Features &amp; Benefits<\/strong><\/li>\n<\/ul>\n<p>Human Aldehyde dehydrogenase (ALDH) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALDH2. No significant cross-reactivity or interference between Human ALDH2 and analogues was observed.<\/p>\n<ul>\n<li><strong>Calibration range<\/strong><\/li>\n<\/ul>\n<p>Please inquire<\/p>\n<ul>\n<li><strong>Limit of detection<\/strong><\/li>\n<\/ul>\n<p>Please inquire<\/p>\n<ul>\n<li><strong>Usage notes<\/strong><\/li>\n<li>Do not mix components from different kit lots or use reagents beyond the kit expiration date.<br \/>\n\u2022 Allow all reagents to warm to room temperature for at least 30 minutes before opening.<br \/>\n\u2022 Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.<br \/>\n\u2022 Unused wells must be kept desiccated at 4 \u00b0C in the sealed bag provided.<br \/>\n\u2022 Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.<br \/>\n\u2022 It is recommended that all samples and standards be assayed in duplicate or triplicate.<\/li>\n<\/ul>\n<p><strong>Alternative names<\/strong><\/p>\n<p>ALDH2; ALDH-E2; ALDHI; ALDM; MGC1806; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; mitochondrial aldehyde dehydrogenase 2; nucleus-encoded mitochondrial aldehyde dehydrogenase 2<\/p>\n<p>&nbsp;<\/p>\n<p>\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805<\/p>\n<ul>\n<li>\u8acb\u52ff\u6df7\u7528\u4e0d\u540c\u8a66\u5291\u76d2\u4e2d\u7684\u7d44\u5206\uff0c\u6216\u5728\u8a66\u5291\u76d2\u5230\u671f\u65e5\u671f\u4e4b\u5f8c\u4f7f\u7528\u8a66\u5291\u3002<\/li>\n<li>\u6253\u958b\u4e4b\u524d\uff0c\u8b93\u6240\u6709\u8a66\u5291\u81f3\u5c11\u52a0\u5ba4\u6eab30\u5206\u9418\u3002<\/li>\n<li>\u7528\u8a66\u5291\u9810\u6e05\u6d17\u79fb\u6db2\u5668\u5438\u982d\uff0c\u70ba\u6bcf\u500b\u6a23\u54c1\uff0c\u6a19\u6e96\u6db2\u548c\u8a66\u5291\u4f7f\u7528\u65b0\u9bae\u7684\u79fb\u6db2\u5668\u5438\u982d\uff0c\u4ee5\u514d\u6c61\u67d3\u3002<\/li>\n<li>\u672a\u4f7f\u7528\u7684\u5b54\u5fc5\u9808\u5728\u63d0\u4f9b\u7684\u5bc6\u5c01\u888b\u4e2d\u65bc4\u00b0C\u4e7e\u71e5\u3002<\/li>\n<li>\u5fb9\u5e95\u6df7\u5408\u5c0d\u65bc\u7d50\u679c\u975e\u5e38\u91cd\u8981\u3002\u5efa\u8b70\u6bcf10\u5206\u9418\u4f7f\u7528\u4f4e\u983b\u632f\u76ea\u5668\u6216\u7a0d\u7a0d\u6416\u52d5\u624b\u3002<\/li>\n<li>\u5efa\u8b70\u6240\u6709\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e00\u5f0f\u5169\u4efd\u6216\u4e00\u5f0f\u4e09\u4efd\u9032\u884c\u6e2c\u5b9a\u3002<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>ALDH2 ELISA\u8a66\u5291\u76d2| Human Aldehyde dehydrogenase (ALDH) ELI [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":24540,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1398],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25878"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25878"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25878\/revisions"}],"predecessor-version":[{"id":25879,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25878\/revisions\/25879"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24540"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25878"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25878"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25878"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}