{"id":25719,"date":"2020-08-12T11:36:08","date_gmt":"2020-08-12T03:36:08","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25719"},"modified":"2022-07-19T16:48:35","modified_gmt":"2022-07-19T08:48:35","slug":"50-kda-%e5%88%86%e5%ad%90%e9%87%8f%e5%8d%b3%e7%94%a8%e5%9e%8bdiaeasy%e9%80%8f%e6%9e%90%e7%ae%a1-%e8%b2%a8%e8%99%9fk1016","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=25719","title":{"rendered":"50 kDa \u5206\u5b50\u91cf\u5373\u7528\u578bDiaEasy\u2122\u900f\u6790\u7ba1 | \u8ca8\u865fK1016"},"content":{"rendered":"

50 kDa \u5206\u5b50\u91cf\u5373\u7528\u578bDiaEasy\u2122\u900f\u6790\u7ba1 | \u8ca8\u865f<\/strong>K1016<\/a><\/span><\/p>\n

BioVision\u7684DiaEasy\u2122\u900f\u6790\u7ba1\u7522\u54c1\u7dda\u70ba\u5927\u591a\u6578\u6b64\u985e\u554f\u984c\u63d0\u4f9b\u4e86\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u7684\u900f\u6790\u7ba1\u5404\u670910\u500b\uff0c25\u500b\u548c100\u500b\u6613\u65bc\u4f7f\u7528\u7684\u5957\u4ef6\uff0c\u53ef\u6eff\u8db31 kDa\u30013.5 kDa\u30016-8 kDa\u300112-14 kDa\u300125 kDa\u548c50 kDa\u4e0d\u540c\u5206\u5b50\u91cf\u622a\u65b7\u7684\u8981\u6c42\u6a23\u54c1\u91cf\u70ba10 \u00b5l\u81f320 ml\u3002\u9019\u4e9b\u5957\u4ef6\u9084\u5305\u542b\u6d6e\u52d5\u67b6\u548c\/\u6216\u652f\u6490\u6258\u76e4\uff0c\u4ee5\u65b9\u4fbf\u8655\u7406\u4ea4\u63db\u7de9\u885d\u6db2\u4e2d\u7684\u8a66\u7ba1\u548c\u9032\u884c\u96fb\u6d17\u812b\u3002 DiaEasy\u2122Dialyzer\u7ba1\u548c\u96fb\u6d17\u812b\u914d\u4ef6\u7684\u7d44\u5408\u70ba\u5f9e\u975e\u8b8a\u6027\u548c\u8b8a\u6027\uff08SDS\uff09\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u81a0(polyacrylamide gels)\u4e2d\u63d0\u53d6\u4efb\u4f55\u86cb\u767d\u8cea\uff0c\u86cb\u767d\u8cea-\u86cb\u767d\u8cea\u6216\u86cb\u767d\u8ceaDNA\u8907\u5408\u7269\uff0c\u4ee5\u53ca\u63d0\u53d6\u5be1\u6838\u82f7\u9178\uff0cRNA\u63d0\u4f9b\u4e86\u7368\u7279\u7684\u5de5\u5177\u4ee5\u53ca\u4f86\u81ea\u805a\u4e19\u70ef\u9170\u80fa\u548c\u74ca\u8102\u7cd6\u51dd\u81a0\u7684DNA\u3002 DiaEasy\u2122\u7ba1\u7684\u819c\u662f\u8d85\u6e05\u6f54\u7684(ultra-clean)\uff0c\u4e0d\u542b\u786b\u548c\u91cd\u91d1\u5c6c(heavy metal free)\uff0c\u4e26\u7d93EDTA\u8655\u7406\uff0c\u4f7f\u6240\u6536\u7a6b\u7684\u86cb\u767d\u8cea\u548c\u6838\u9178\u9069\u5408\u5206\u5b50\u751f\u7269\u5b78\u5be6\u9a57\u3002\u9019\u4e9bDiaEasy\u2122\u7ba1\u53ef\u5be6\u73fe\u5feb\u901f\uff0c\u5b89\u5168\u548c\u7c21\u55ae\u7684simple loading\u548crecovery\u3002\u5b83\u5011\u662f\u975e\u5e38\u9ad8\u6027\u80fd\u7684\u900f\u6790\u7ba1\uff0c\u662f\u5e02\u5834\u4e0a\u6700\u65b9\u4fbf\u7684\u900f\u6790\u7cfb\u7d71\u3002<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> Biovision\u7684DiaEasy\u2122\u900f\u6790\u7ba1\uff0850 kDa MWCO\uff09\u53ef\u4ee5\u7d50\u5408\u5169\u7a2e\u4f5c\u7528\u65b9\u5f0f\uff1a1\uff09\u6a23\u54c1\u9ad4\u7a4d\u57280.1-3 ml\u4e4b\u9593\u7684\u900f\u6790\u6216\u7de9\u885d\u6db2\u4ea4\u63db\uff1b 2\uff09\u5f9e\u805a\u4e19\u70ef\u9170\u80fa(polyacrylamide)\u6216\u74ca\u8102\u7cd6\u51dd\u81a0(agarose gel)\u4e0a\u96fb\u6d17\u812b\u5927\u5206\u5b50(electro-elution)\u3002\u5b83\u5011\u4e5f\u53ef\u4ee5\u7528\u65bc\u5176\u4ed6\u4e00\u4e9b\u76ee\u7684\uff0c\u5982\u4e0b\u6240\u793a\u3002\u8a72\u8a2d\u5099\u53ef\u5728\u4efb\u4f55\u4e00\u7a2e\u6a21\u5f0f\u4e0b\u5be6\u73fe\u5feb\u901f\u9ad8\u6548\u7684\u6027\u80fd\uff0c\u4e26\u63d0\u53d6\u5927\u5206\u5b50\u800c\u6c92\u6709\u4efb\u4f55\u6c61\u67d3\u3002\u901a\u904e\u66f4\u63db\u5957\u4ef6\u96a8\u9644\u7684\u84cb\u5b50\uff0cDiaEasy\u2122Dialyzer\u7ba1\u53ef\u4ee5\u8f15\u9b06\u8abf\u6574\u900f\u6790\u91cf\uff0c\u7bc4\u570d\u57280.1-3 ml\u4e4b\u9593\u3002 DiaEasy\u2122\u7ba1\u7684\u819c\u662f\u8d85\u6e05\u6f54\u7684\uff0c\u4e0d\u542b\u786b\u548c\u91cd\u91d1\u5c6c\uff0c\u4e26\u4e14\u7d93\u904eEDTA\u8655\u7406\uff0c\u4f7f\u5176\u9069\u5408\u5206\u5b50\u751f\u7269\u5b78\u5be6\u9a57\u3002\u9019\u4e9b\u7ba1\u7d93\u9ad8\u58d3\u6ec5\u83cc\uff0c\u7121\u7d30\u83cc\u3002 DiaEasy\u2122\u900f\u6790\u7ba1\u53ef\u5be6\u73fe\u5feb\u901f\uff0c\u5b89\u5168\u548c\u7c21\u55ae\u7684loading\u548crecovery\u3002\u5b83\u5011\u662f\u975e\u5e38\u9ad8\u6027\u80fd\u7684\u900f\u6790\u7ba1\uff0c\u662f\u5e02\u5834\u4e0a\u6700\u65b9\u4fbf\u7684\u900f\u6790\u7cfb\u7d71\u3002\u900f\u6790\u5f8c\u7684\u6a23\u54c1\u8cea\u91cf\u53ef\u4ee5\u901a\u904e\u86cb\u767d\u8cea\u548c\u6838\u9178\u5e38\u7528\u7684\u5e7e\u7a2e\u6aa2\u6e2c\u65b9\u6cd5\u9032\u884c\u6aa2\u67e5\u3002<\/p>\n

\u5373\u7528\u578b\u900f\u6790\u7ba1\u61c9\u7528<\/strong>:<\/p>\n

    \n
  1. Dialysis or buffer exchange of sample volumes up to 3 ml.<\/li>\n
  2. Preparation of protein samples for MALDI-MS.<\/li>\n
  3. Samples concentration.<\/li>\n
  4. Peptide dialysis as small as 10 amino acids.<\/li>\n
  5. Virus-particle purification.<\/li>\n
  6. Removal of contaminating micro molecules.<\/li>\n
  7. Tissue culture extracts purification.<\/li>\n
  8. Removal of salts, surfactants, solvents and detergents.<\/li>\n
  9. Complex formation studies (protein-protein, protein-DNA and protein-RNA).<\/li>\n
  10. pH and buffer adjustment of sample solution, protein extract or cell extract.<\/li>\n
  11. High through put dialysis (using a single beaker with several DiaEasy\u2122 tubes on one floating pad)<\/li>\n<\/ol>\n

    \u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/p>\n

     <\/p>\n

     <\/p>\n\n\n\n\n\n\n\n\n
    <\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
    \u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
    \u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
    \u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

    \u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

    \u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

    \u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

    \u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

    \u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

     <\/td>\n<\/tr>\n

    \u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
    \u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

    50 kDa \u5206\u5b50\u91cf\u5373\u7528\u578bDiaEasy\u2122\u900f\u6790\u7ba1 | \u8ca8\u865fK1016 BioVision\u7684DiaEasy\u2122\u900f\u6790 […]<\/p>\n","protected":false},"author":3,"featured_media":1098,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1418],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25719"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25719"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25719\/revisions"}],"predecessor-version":[{"id":25721,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25719\/revisions\/25721"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/1098"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25719"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25719"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25719"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}