{"id":25191,"date":"2020-06-04T12:14:11","date_gmt":"2020-06-04T04:14:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25191"},"modified":"2025-06-17T16:48:17","modified_gmt":"2025-06-17T08:48:17","slug":"%e9%81%8e%e6%b0%a7%e5%8c%96%e7%89%a9%e9%85%b6%ef%bc%88peroxidases%ef%bc%89%e5%88%86%e6%9e%90%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fktb1060","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=25191","title":{"rendered":"\u904e\u6c27\u5316\u9176\uff08Peroxidases, POD\uff09\u5206\u6790\u8a66\u5291\u76d2| CheKine\u2122 Micro Peroxidase (POD) Activity Assay Kit\u8ca8\u865fKTB1150"},"content":{"rendered":"

POD\u904e\u6c27\u5316\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2| \u8ca8\u865fKTB1150<\/span><\/strong><\/span><\/a><\/span><\/p>\n

CheKine\u2122 Micro Peroxidase (POD) Activity Assay Kit<\/p>\n

POD\u904e\u6c27\u5316\u9176(Peroxidase)\u5ee3\u6cdb\u5b58\u5728\u65bc\u690d\u7269\u9ad4\u5167\u7684\u4e0d\u540c\u7d44\u7e54\uff0c\u5c6c\u6d3b\u6027\u8f03\u9ad8\u7684\u8a98\u5c0e\u9176\uff0c\u80fd\u5920\u53cd\u61c9\u690d\u7269\u7684\u4ee3\u8b1d\u72c0\u6cc1\u548c\u5c0d\u74b0\u5883\u7684\u9069\u61c9\u6027\u3002 POD \u904e\u6c27\u5316\u9176(Peroxidase)\u5177\u6709\u96d9\u91cd\u7684\u4f5c\u7528\u7279\u6027\uff0c\u5728\u9006\u5883\u6216\u8870\u8001\u521d\u671f\u7684\u8868\u73fe\u70ba\u6e05\u9664 H2<\/sub>O2<\/sub> \uff0c\u9054\u5230\u4fdd\u8b77\u7d30\u80de\u7684\u529f\u80fd\u3002\u4f46\u5728\u9006\u5883\u6216\u8870\u8001\u5f8c\u671f\uff0c\u5247\u53c3\u8207\u6d3b\u6027\u6c27\u7684\u751f\u6210\u3001\u8449\u7da0\u7d20\u7684\u964d\u89e3\uff0c\u5f15\u767c\u819c\u8102\u904e\u6c27\u5316\u4f5c\u7528\uff0c\u52a0\u901f\u7d30\u80de\u7684\u8870\u8001\u6b7b\u4ea1\uff0c\u6210\u70ba\u690d\u7269\u7684\u8870\u8001\u6307\u6a19\u3002\u690d\u7269\u9ad4\u6e05\u9664\u6d3b\u6027\u6c27\u7684\u4fdd\u8b77\u7cfb\u7d71\u53c8\u7a31\u70ba\u6297\u6c27\u5316\u7cfb\u7d71\uff0c \u5305\u62ec\u6297\u6c27\u5316\u9175\u7d20\u53ca\u975e\u9175\u7d20\u578b\u6297\u6c27\u5316 \u5291 \uff08\u8868 2 \uff09\uff0c\u4ee5\u61c9\u4ed8\u5177\u64f4\u6563\u6027\u3001\u6c34\u6eb6\u6027\u53ca\u53ef\u8207\u5404\u7a2e\u751f\u7269\u5206\u5b50\u53cd\u61c9\u4e4b\u6d3b\u6027\u6c27\u5316\u7269\uff0c\u907f\u514d\u767c\u751f\u4f7f\u6d3b\u6027\u7269\u7a2e\u6bd2\u6027\u52a0\u5f37\u7684\u9023\u9396\u53cd\u61c9\u3002(\u51fa\u81ea\u8fb2\u696d\u90e8\u690d\u7269\u5c0d\u74b0\u5883\u9006\u5883\u4e4b\u8abf\u63a7\u8207\u61c9\u7528100\u5e749\u6708(\u7b2c231\u671f)\u3002<\/p>\n

<\/p>\n

\u904e\u6c27\u5316\u9176\uff08Peroxidases , EC\u7de8\u865f1.11.1.x\uff09\u662f\u4e00\u5927\u985e\u9176\uff0c\u901a\u5e38\u50ac\u5316\u4ee5\u4e0b\u5f62\u5f0f\u7684\u53cd\u61c9\uff1aROOR’+\u96fb\u5b50\u7d66\u9ad4\uff082 e-\uff09+ 2H +\u2192ROH + R’OH\u3002\u5c0d\u65bc\u9019\u4e9b\u9176\u4e2d\u7684\u8a31\u591a\u9176\uff0c\u6700\u4f73\u5e95\u7269\u662f\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\uff0c\u4f46\u5176\u4ed6\u9176\u5247\u5c0d\u6709\u6a5f\u6c2b\u904e\u6c27\u5316\u7269(organic hydroperoxides)\uff08\u4f8b\u5982\u8102\u8cea\u904e\u6c27\u5316\u7269, lipid peroxides\uff09\u66f4\u5177\u6d3b\u6027\u3002\u904e\u6c27\u5316\u7269\u9176(Peroxidases)\u53ef\u5728\u5176\u6d3b\u6027\u4f4d\u9ede(active sites)\u5305\u542b\u8840\u7d05\u7d20\u8f14\u56e0\u5b50(heme cofactor)\uff0c\u6216\u5728\u6c27\u5316\u9084\u539f\u6d3b\u6027\u7684\u534a\u80f1\u6c28\u9178(redox-active cysteine)\u6216\u7852\u4ee3\u534a\u80f1\u6c28\u9178\u6b98\u57fa(selenocysteine residues)\u4e0a\u5177\u6709\u66ff\u4ee3\u6027\u3002\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidase \uff09\u5076\u806f\u7269\u88ab\u5ee3\u6cdb\u7528\u4f5cELISA\uff0c\u514d\u75ab\u7d44\u7e54\u5316\u5b78\u6280\u8853\u4ee5\u53caNorthern\uff0cSouthern\u548cWestern\u5370\u8de1\u5206\u6790\u7684\u8f14\u52a9\u6aa2\u6e2c\u8a66\u5291\u3002\u904e\u6c27\u5316\u7269\u9176\uff0cPOD\uff08EC 1.11.1.7\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u4e2d\u3002\u80fd\u50ac\u5316\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u3001\u915a\u985e( phenols )\u548c\u80fa\u985e\u5316\u5408\u7269(amine compounds)\u7684\u6c27\u5316\uff0c\u4e26\u6d88\u9664\u904e\u6c27\u5316\u6c2b(hydrogen peroxide)\u3001\u915a\u985e(phenols )\u548c\u80fa\u985e(amines)\u3002\u6bd2\u6027\u7684\u96d9\u91cd\u4f5c\u7528\u3002CheKine\u2122 Micro\u904e\u6c27\u5316\u9176\u5206\u6790\u8a66\u5291\u76d2\uff0c\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u6aa2\u6e2c\u690d\u7269\u7d44\u7e54(plant tissues)\u3001\u7d30\u83cc(bacteria)\u3001\u771f\u83cc(fungus )\u6216\u7d30\u80de\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u672c\u4e2d POD \u6d3b\u6027\u7684\u7c21\u55ae\u65b9\u6cd5\u3002\u5728\u6e2c\u5b9a\u4e2d\uff0cPOD\u900f\u904eH2O2\u50ac\u5316\u7279\u5b9a\u5e95\u7269\u7684\u6c27\u5316\uff0c\u7522\u751f\u5728460nm\u8655\u5177\u6709\u7279\u5fb5\u5149\u5438\u6536\u7684\u6709\u8272\u7269\u8cea\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Micro Peroxidase (POD) Activity Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nThe kit provides a simple method for detecting POD activity in a variety of biological samples such as plant tissues,bacteria, fungus or cells . In the assay,POD catalyzes the oxidation of specific substrates by H2O2 to produce colored substances with characteristic light absorption at 460nm.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nperoxide reductases<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Extraction Buffer
\n\u2022 Substrate
\n\u2022 H2O2<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of POD activity in a variety of biological samples such as plant tissues,bacteria, fungus or cells .
\n\u2022 Detailed sample preparation and result calculation methods are provided.
\n\u2022 Simple operation steps.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.
\n\u2022 Avoid foaming or bubbles when mixing or reconstituting components.
\n\u2022 Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before starting the assay.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nPeroxidase, POD (EC 1.11.1.7) is widely present in animals, plants, microorganisms and cultured cells. It can catalyze hydrogen peroxide, oxidation of phenols and amine compounds, and eliminate hydrogen peroxide, phenols and amines. The dual effect of toxicity.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nperoxide reductases<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

POD\u904e\u6c27\u5316\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2| \u8ca8\u865fKTB1150 CheKine\u2122 Micro Per […]<\/p>\n","protected":false},"author":3,"featured_media":24734,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1502],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25191"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25191"}],"version-history":[{"count":11,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25191\/revisions"}],"predecessor-version":[{"id":51286,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25191\/revisions\/51286"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24734"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25191"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25191"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25191"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}