{"id":25114,"date":"2020-06-01T16:47:59","date_gmt":"2020-06-01T08:47:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25114"},"modified":"2023-11-27T16:29:23","modified_gmt":"2023-11-27T08:29:23","slug":"edu-%e7%b4%b0%e8%83%9e%e5%a2%9e%e6%ae%96%e8%a9%a6%e5%8a%91%e7%9b%92exem-546565-nm-cell-proliferation-edu-image-kit-%e8%b2%a8%e8%99%9fkta2031","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=25114","title":{"rendered":"EdU \u7d30\u80de\u589e\u6b96\u8a66\u5291\u76d2(\u6a58) | Cell Proliferation EdU Image Kit \u8ca8\u865fKTA2031"},"content":{"rendered":"

\u3010DNA\u5408\u6210\/\u7d30\u80de\u589e\u6b96\u6aa2\u6e2c\u3011\u00a0EdU Image\u8a66\u5291\u76d2-BrdU\u5206\u6790\u65b0\u578b\u66ff\u4ee3\u65b9\u6cd5<\/a><\/span><\/p>\n

\u7d30\u80de\u589e\u6b96(cell proliferation)\u7684\u6aa2\u6e2c\uff0c\u5c0d\u65bc\u8a55\u4f30\u7d30\u80de\u5065\u5eb7(cell health)\uff0c\u78ba\u5b9a\u907a\u50b3\u6bd2\u6027(genotoxicity)\u6216\u8a55\u4f30\u6297\u764c\u85e5\u7269(anticancer drugs)\u81f3\u95dc\u91cd\u8981\u3002\u5230\u76ee\u524d\u70ba\u6b62\uff0c\u76f4\u63a5\u6e2c\u91cfDNA\u5408\u6210(DNA synthesis)\u662f\u6700\u6e96\u78ba\u7684\u65b9\u6cd5\uff0c\u901a\u5e38\u662f\u901a\u904e\u5728\u8907\u88fd\u904e\u7a0b\u4e2d\u5c07[3H]\u80f8\u82f7(thymidine)\u62165-\u6eb4-2′-\u812b\u6c27\u5c3f\u82f7(5-bromo-2\u2019-deoxyuridine)\u7b49\u6838\u82f7\u985e\u4f3c\u7269(nucleoside analog like)\u647b\u5165\u7d30\u80de\u4e2d\uff0c\u7136\u5f8c\u901a\u904e\u653e\u5c04\u81ea\u986f\u5f71\u6216\u5206\u5225\u4f7f\u7528\u6297BrdU\u6297\u9ad4(anti-BrdU-antibody)\u3002EdU\uff085-\u4e59\u7094\u57fa-2′-\u812b\u6c27\u5c3f\u82f7\uff09\u662fBrdU\uff085-bromo-2′-\u812b\u6c27\u5c3f\u82f7\uff09\u7684\u65b0\u578b\u66ff\u4ee3\u54c1\u3002\u5b83\u88ab\u53d6\u4ee3\u80f8\u82f7\u647b\u5165\u5230\u65b0\u5408\u6210\u7684\u589e\u6b96\u7d30\u80deDNA\u4e2d\u3002\u5c075-EdU\u647b\u5165\u5230\u589e\u6b96\u7d30\u80de\u4e2d\uff0c\u7136\u5f8c\u4f7f\u7528\u9ede\u64ca\u9023\u63a5\u7528\u87a2\u5149\u758a\u6c2e\u5316\u7269\u9032\u884c\u6aa2\u6e2c\u3002\u8207BrdU\u7684\u4f7f\u7528\u76f8\u53cd\uff0c\u65b0\u65b9\u6cd5\u4e0d\u9700\u8981\u6a23\u54c1\u56fa\u5b9a\u6216DNA\u8b8a\u6027\u3002\u53ef\u7528\u65bc\u7814\u7a76\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71\u4e2d\u7684\u7d30\u80de\u589e\u6b96\u3002<\/p>\n

EdU \u647b\u5165\u548c\u6aa2\u6e2c\u4e0d\u9700\u8981 DNA \u8b8a\u6027\uff0c\u5f9e\u800c\u6e1b\u5c11\u6b65\u9a5f\u4e26\u6e1b\u5c11\u7d44\u7e54\u964d\u89e3\u3002<\/p>\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0|\u00a0<\/strong>Cell Proliferation EdU Image Kit\u00a0\u00a0(Orange Fluorescence)\u00a0\u8ca8\u865fKTA2031<\/a><\/span><\/p>\n

\"\"<\/p>\n

\u6700\u6e96\u78ba\u7684\u589e\u6b96\u6aa2\u6e2c\u65b9\u6cd5( proliferation-detection methods)\u662f\u57fa\u65bc\u65b0\u5408\u6210 DNA \u4e2d\u6838\u82f7\u985e\u4f3c\u7269(nucleoside analogues)\u7684\u647b\u5165\u548c\u6e2c\u91cf\uff0c\u5176\u4e2d\u6eb4\u812b\u6c27\u5c3f\u82f7 (bromodeoxyuridine, BrdU) \u662f\u5e38\u7528\u7684\u985e\u4f3c\u7269\u3002\u4ee5\u56b4\u82db\u65b9\u6cd5\uff08HCl\u3001\u71b1\u6216\u9175\u7d20\uff09\u4f7f DNA \u8b8a\u6027\u4ee5\u66b4\u9732 BrdU \u5206\u5b50\u5f8c\uff0c\u4f7f\u7528\u6297 BrdU \u6297\u9ad4\u5c0d BrdU \u6a19\u8a18\u7684 DNA \u9032\u884c\u5b9a\u91cf\u3002\u6b64\u6b65\u9a5f\u975e\u5e38\u8017\u6642\u4e14\u96e3\u4ee5\u4e00\u81f4\u5730\u57f7\u884c\u3002\u56b4\u53b2\u7684\u8655\u7406\u4e5f\u6703\u5c0d\u6a23\u672c\u7684\u5b8c\u6574\u6027\u548c\u54c1\u8cea\u7522\u751f\u4e0d\u5229\u5f71\u97ff\uff0c\u9019\u4f7f\u5f97\u8207\u5176\u4ed6\u6297\u9ad4\u5171\u67d3\u8272\u5177\u6709\u6311\u6230\u6027\u3002\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2(Cell Proliferation EdU Image Kit, \u8ca8\u865fKTA2031)\uff08 (Orange Fluorescence)\uff09\u662fBrdU\u5206\u6790\u7684\u4e00\u7a2e\u65b0\u578b\u66ff\u4ee3\u65b9\u6cd5\u3002 EdU\uff085-\u4e59\u7094\u57fa-2\u2032-\u812b\u6c27\u5c3f\u82f7, EdU (5-ethynyl-2′-deoxyuridine)\u00a0\uff09\u662f\u80f8\u82f7\u7684\u6838\u82f7\u985e\u4f3c\u7269\uff0c\u5728\u6d3b\u6027DNA\u5408\u6210\u904e\u7a0b\u4e2d\u88ab\u647b\u5165DNA\u4e2d\u3002\u8207BrdU\u5206\u6790\u76f8\u6bd4\uff0cEdU-Click\u5206\u6790\u4e0d\u662f\u57fa\u65bc\u6297\u9ad4\u7684\uff0c\u56e0\u6b64\u4e0d\u9700\u8981DNA\u8b8a\u6027(denaturation)\uff08\u901a\u5e38\u4f7f\u7528HCl\u6216\u52a0\u71b1\u6216\u7528DNase\u6d88\u5316\uff09\u4f86\u6aa2\u6e2c\u647b\u5165\u7684\u6838\u82f7\u3002\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u4e0d\u50c5\u6613\u65bc\u4f7f\u7528\uff0c\u800c\u4e14\u66f4\u6e96\u78ba\u6aa2\u6e2c\u57fa\u65bcclick reaction\uff0c\u758a\u6c2e\u5316\u7269\u548c\u7094\u70f4\u4e4b\u9593\u7684\u9285\u50ac(copper-catalyzed)\u5316\u5171\u50f9\u53cd\u61c9\u572830\u5206\u9418\u5167\u5b8c\u6210\u3002\u53ef\u7528\u65bc\u986f\u5fae\u93e1\u6210\u50cf\u3001\u6d41\u5f0f\u7d30\u80de\u5100\u548c\u9ad8\u901a\u91cf\u7be9\u9078\u3002<\/p>\n

 <\/p>\n

<\/p>\n

(\u6587\u737b\u4f86\u6e90<\/a><\/span>) TUC338 knockdown inhibits DLBCL cell proliferation. (a) Establishment of two DLBCL cell lines stably knocking down of TUC338. (b\u2013g) Soft agar colony, EdU, and CCK-8 assays detecting colony formation, DNA synthesis, and cell viability, respectively. Scale bar\u2009=\u200950\u2009\u03bc<\/em>M,\u00a0\u2217\u2217<\/em><\/sup>P<\/em>\u00a0< 0.01,\u00a0\u2217\u2217\u2217<\/em><\/sup>P<\/em>\u00a0< 0.001.<\/p>\n

<\/p>\n

(\u6587\u737b\u4f86\u6e90<\/a><\/span>) GLUT12 overexpression promotes GC proliferation and attenuates responses to everolimus.\u00a0A<\/b>\u00a0The WB analysis to show GLUT12 expression of SGC-7901 and HGC-27 cells that were infected with lentivirus carrying vectors or GLUT12 overexpression plasmids.\u00a0B-D<\/b>\u00a0The CCK-8 (B<\/b>), colony formation (C<\/b>) and EdU (D<\/b>) assays to measure proliferation of cells as in (A<\/b>).<\/p>\n

<\/p>\n

(\u6587\u737b\u4f86\u6e90<\/a><\/span>) Effects of low dose GO on the proliferation of PC3 cells\u00a0in vitro<\/em>. (a) Representative images of the cell proliferation assay for PC3 cells under GO nanosheet (GO-L, GO-M, and GO-S) treatment at 0, 5, 10, 15, 20, and 50\u00a0\u03bcg\/mL for 24\u00a0h with EdU doped. (b) Statistical data of the EdU positive ratios, n\u00a0=\u00a05. Scale bar\u00a0=\u00a0500\u00a0\u03bcm. (c) Western blotting of expression levels of the PI3K\/AKT\/mTOR signal pathway-related proteins in PC3 cells post GO nanosheet (GO-L, GO-M, and GO-S) treatment at 0, 2, 5, 10, 15, 20, and 50\u00a0\u03bcg\/mL for 24\u00a0h *: P\u00a0<\u00a00.05, **: P\u00a0<\u00a00.001, relative to untreated control.<\/p>\n

<\/p>\n

(\u6587\u737b\u4f86\u6e90<\/a><\/span>)\u00a0Sitagliptin inhibits proliferation and induces apoptosis in immortalized glioma cells. (A-D) EdU and Colony formation assays were performed to evaluate the effect of gradient concentrations of Sitagliptin on cell proliferation in T98G and U251 cells. Representative EdU and colony images are shown in A and C, respectively. Quantitative analyses of the percentage of EdU-positive cells and the number of colonies are shown in B and D, respectively. (E-H) Flow cytometry and TUNEL assays were used to analyze the effect of gradient concentrations of Sitagliptin on cell apoptosis in T98G and U251 cells. Representative flow cytometry histograms and TUNEL images are shown in E and G, respectively. Quantitative analyses of the percentage of apoptotic cells and the percentage of TUNEL-positive cells are shown in F and H, respectively. Scale bars: 200\u2009\u00b5m. **P<\/em>\u2009<\u20090.01, ***P<\/em>\u2009<\u20090.001.<\/p>\n

<\/p>\n

(\u6587\u737b\u4f86\u6e90<\/a><\/span>)\u00a0 Knockdown of CKAP2L expression inhibits KIRC cell proliferation (scale bar: 500 \u00b5m. *p\u2009<\u20090.05; **p\u2009<\u20090.01; ***p\u2009<\u20090.001; ****p\u2009<\u20090.0001). (A<\/b>,B<\/b>) Protein blotting images and quantitative data showed that the expression of CKAP2L in A498 and 786-O cells was significantly reduced after siRNA transfection. The uncropped original image can be obtained in Supplementary Fig. S7. (C<\/b>) CCK8 assay detects the change in the proliferation ability of A498 and 786-O cells after CKAP2L knockdown. (D<\/b>,E<\/b>) Clone formation assay examined the change in the proliferation ability of A498 and 786-O cells after CKAP2L knockdown. (F<\/b>,G<\/b>) EdU incorporation assays assess the change in the proliferation ability of A498 and 786-O cells after CKAP2L knockdown.<\/p>\n

 <\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

Specification<\/span><\/p>\n\n\n\n\n
Product name<\/td>\nEdU Cell Proliferation Image Kit (Orange Fluorescence)<\/a><\/td>\n<\/tr>\n
Applications notes<\/td>\nCell Proliferation EdU Image Kit (Orange Fluorescence) is a novel alternative to the BrdU assay. EdU (5-ethynyl-2\u00b4-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis. Comparing to BrdU assays, the EdU-Click Assays are not antibody based and therefore do not require DNA denaturation (typically using HCl or heat or digestion with DNase) for detection of the incorporated nucleoside. Detection is based on a click reaction, a copper-catalyzed covalent reaction between an azide and an alkyne, is complete within 30 minutes.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Properties<\/span><\/p>\n\n\n\n\n\n\n\n
Kit components<\/td>\n\u2022 EdU (10mM)
\n\u2022BSA Wash Solution (5\u00d7)
\n\u2022AbFluor 545 azide
\n\u2022Reaction buffer (10\u00d7)
\n\u2022Copper Reagent
\n\u2022Reducing Agent
\n\u2022Hoechst 33342 (1000\u00d7)
\n\u2022Hydroxyurea<\/td>\n<\/tr>\n
Features & Benefits<\/td>\n\u2022 Optimized negative control is provided to rule out false positives
\n\u2022No antibody needed.
\n\u2022 No denaturation steps, preservation of cell morphology and DNA integrity.
\n\u2022 Simple, reliable and time-saving than traditional methods.
\n\u2022 Proprietary AbFluor 545 azide (Ex\/Em = 546\/565 nm)-good photostability and minimizing fluorescence quenching.
\n\u2022 Optimized for fluorescent microscopy.<\/td>\n<\/tr>\n
Storage instructions<\/td>\nStable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.<\/td>\n<\/tr>\n
Shipping<\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Additional Information<\/span><\/p>\n\n\n\n
Background<\/td>\nThe detection of cell proliferation is of utmost importance for assessing cell health, determining genotoxicity or evaluating anticancer drugs. Until now, measuring DNA synthesis directly is most accurate method of doing it, normally performed by incorporation of the nucleoside analog like [3H] thymidine or 5-bromo-2\u2019-deoxyuridine to cells during replication, and then detected or visualized by autoradiography or with an anti-BrdU-antibody respectively.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u3010DNA\u5408\u6210\/\u7d30\u80de\u589e\u6b96\u6aa2\u6e2c\u3011\u00a0EdU Image\u8a66\u5291\u76d2-BrdU\u5206\u6790\u65b0\u578b\u66ff\u4ee3\u65b9\u6cd5 \u7d30\u80de\u589e\u6b96(cell prol […]<\/p>\n","protected":false},"author":3,"featured_media":25118,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[1511],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25114"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=25114"}],"version-history":[{"count":10,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25114\/revisions"}],"predecessor-version":[{"id":47382,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/25114\/revisions\/47382"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25118"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=25114"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=25114"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=25114"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}