{"id":25047,"date":"2020-05-29T13:55:18","date_gmt":"2020-05-29T05:55:18","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=25047"},"modified":"2022-07-19T17:02:02","modified_gmt":"2022-07-19T09:02:02","slug":"fucaz%e4%bf%ae%e9%a3%be%e7%b3%96%e8%9b%8b%e7%99%bd%e6%b8%ac%e5%ae%9a-ezclick-fucose-fucaz-modified-glycoprotein-assay-%e8%b2%a8%e8%99%9fk592","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=25047","title":{"rendered":"FucAz\u4fee\u98fe\u7cd6\u86cb\u767d\u6e2c\u5b9a | EZClick\u2122 Fucose (FucAz) Modified Glycoprotein Assay \u8ca8\u865fk592"},"content":{"rendered":"

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EZClick\u2122Fucose\uff08FucAz\uff09\u4fee\u98fe\u7684\u7cd6\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>k592<\/a><\/span><\/p>\n

EZClick\u2122 Fucose (FucAz) Modified Glycoprotein Assay Kit (FACS\/Microscopy, Green Fluorescence)<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u63cf\u8ff0:<\/strong> \u805a\u91a3(Glycans)\u662f\u751f\u547d\u6240\u6709\u9818\u57df\u4e2d\u7cd6\u86cb\u767d(glycoproteins)\uff0c\u91a3\u8102(glycolipids)\u548c\u86cb\u767d\u805a\u91a3(proteoglycans)\u7684\u91cd\u8981\u7d44\u6210\u90e8\u5206\u3002\u7cd6\u57fa\u5316\u5728> 50\uff05\u7684\u771f\u6838\u86cb\u767d\u4e0a\u5171\u7ffb\u8b6f\u6216\u7ffb\u8b6f\u5f8c(post-translationally)\u767c\u751f\uff0c\u5728\u7d30\u80de\u904e\u7a0b\u819c\u76f8\u95dc(membrane-associated)\uff0c\u7d30\u80de\u5167\u6216\u5206\u6ccc\u7684\u7cd6\u86cb\u767d(secreted glycoproteins)\uff0c\u86cb\u767d\u8cea\u751f\u7269\u6d3b\u6027(protein bioactivity)\u548c\u4ee3\u8b1d\u8f49\u63db(metabolic turnover)\u4e2d\u81f3\u95dc\u91cd\u8981\u3002\u7cd6\u86cb\u767d(Glycoproteins)\u6309\u78b3\u6c34\u5316\u5408\u7269(carbohydrate)\u548c\u6c28\u57fa\u9178\u9023\u63a5\u4f4d\u9ede(amino acid linkage site)\u7684\u985e\u578b\u5206\u7d44\u3002\u5ca9\u85fb\u7cd6(Fucose)\u5b58\u5728\u65bc\u810a\u690e\u52d5\u7269(vertebrates)\uff0c\u7121\u810a\u690e\u52d5\u7269(invertebrates)\uff0c\u690d\u7269(plants)\u548c\u7d30\u83cc\u4e2d(bacteria)\u5b58\u5728\u7684\u7cd6\u86cb\u767d(glycoproteins)\u548c\u91a3\u8102(glycolipids)\u4e2d\u3002\u5b83\u901a\u5e38\u662f\u53c3\u8207\u7d30\u80de\u9593\u4fe1\u865f\u50b3\u905e(cell\u2013cell signaling)\u548c\u9077\u79fb(migration)\u7684\u805a\u91a3(glycans)\u4e2d\u7684\u672b\u7aef\u7cd6(terminal sugar)\uff0c\u53c3\u8207\u751f\u7406(fertilization)\u548c\u75c5\u7406\u904e\u7a0b(pathological)\uff0c\u4f8b\u5982\u53d7\u7cbe(fertilization)\uff0c\u80da\u80ce\u9aee\u751f(embryogenesis)\uff0c\u6dcb\u5df4\u7d30\u80de\u904b\u8f38(lymphocyte trafficking)\uff0c\u514d\u75ab\u53cd\u61c9(immune responses)\u548c\u764c\u75c7\u8f49\u79fb(cancer metastasis)\u3002\u5927\u591a\u6578\u9032\u884c\u5ca9\u85fb\u7cd6\u57fa\u5316(fucosylation)\u7684\u9776\u86cb\u767d\u662f\u7d30\u80de\u8868\u9762\u7684\u5206\u6ccc\u86cb\u767d\u6216\u819c\u86cb\u767d\u3002\u5ca9\u85fb\u7cd6\u57fa\u5316(Fucosylation)\u662f\u6d89\u53ca\u764c\u75c7(cancer)\u548c\u708e\u75c7(inflammation.)\u7684\u6700\u91cd\u8981\u7684\u4fee\u98fe\u4e4b\u4e00\u3002\u5e7e\u7a2e\u542b\u6709\u5ca9\u85fb\u7cd6\u7684\u751f\u7269\u6a19\u8a18\u7269(biomarkers)\u8207\u764c\u75c7\u6709\u95dc\uff0c\u4f8b\u5982\u03b1-\u7532\u80ce\u86cb\u767d\uff08alpha-fetoprotein , AFP\uff09\u7528\u65bc\u8a3a\u65b7\u809d\u7d30\u80de\u764c(hepatocellular carcinoma)\uff0c\u8868\u9762\u7cd6\u9798\u8102Globo H(glycosphingolipid Globo H)\u662f\u5728\u4e73\u817a\u764c(breast)\uff0c\u524d\u5217\u817a\u764c(prostate)\u548c\u5375\u5de2\u764c(ovarian cancers)\u7684\u7d30\u80de\u8868\u9762\u4e0a\u767c\u73fe\u7684\u6297\u539f\u6c7a\u5b9a\u7c07(epitope)\u3002\u7531\u65bc\u7cd6\u86cb\u767d(glycoproteins)\u4e0d\u662f\u76f4\u63a5\u5728\u57fa\u56e0\u7d44\u4e2d\u7de8\u78bc\u7684\uff0c\u56e0\u6b64\u5c0d\u5176\u8868\u5fb5\u548c\u5206\u6790\u7684\u65b9\u6cd5\u975e\u5e38\u611f\u8208\u8da3\u3002\u56e0\u6b64\uff0cBioVision\u63d0\u4f9b\u4e86EZClick\u2122Fucose\uff08FucAz\uff09\u4fee\u98fe\u7684\u7cd6\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(BioVision offers EZClick\u2122 Fucose (FucAz) Modified Glycoprotein Assay Kit< k\uff0ck592) \u9019\u662f\u4e00\u7a2e\u9ad8\u5ea6\u7279\u7570\u6027\uff0c\u7c21\u55ae\u800c\u53ef\u9760\u7684\u65b9\u6cd5\uff0c\u7528\u65bc\u6a19\u8a18\u548c\u6aa2\u6e2c\u7d30\u80de\u4e2d\u7684\u5ca9\u85fb\u7cd6\u57fa\u5316\u86cb\u767d(fucosylated proteins)\u3002\u6211\u5011\u4f7f\u7528\u5ca9\u85fb\u91a3\u985e\u4f3c\u7269(fucose analog)\uff0c\u5c07\u5176\u76f4\u63a5\u9935\u5165\u7d30\u80de\uff0c\u901a\u904e\u5ca9\u85fb\u7cd6\u633d\u6551\u9014\u5f91(fucose salvage pathway)\u9032\u884c\u8655\u7406\uff0c\u4e26\u6574\u5408\u5230\u7cd6\u86cb\u767d\u4e2d\u3002\u96a8\u5f8c\u8207\u542b\u7094\u70f4\u7684\u67d3\u6599(alkyne-containing dye)\u767c\u751f\u9ede\u64ca\u53cd\u61c9\uff0c\u8a72\u7cfb\u7d71\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5f37\u5927\u7684\u65b9\u6cd5\u4f86\u5c0d\u805a\u91a3\u7684\u5b9a\u4f4d(localization) \uff0c\u904b\u8f38(trafficking)\u548c\u52d5\u529b\u5b78(dynamics0\u9032\u884c\u6210\u50cf\uff0c\u6216\u901a\u904eFACS\u6aa2\u6e2c\u9032\u884c\u5b9a\u91cf\u7814\u7a76\u3002\u53ef\u4ee5\u4f7f\u7528\u9069\u7576\u7684\u6fc0\u767c\u6e90\u57281D\u62162D\u51dd\u81a0\u4e2d\u76f4\u63a5\u6aa2\u6e2c\u5ca9\u85fb\u7cd6\u6a19\u8a18\u7684\u7cd6\u86cb\u767d\uff0c\u6216\u8005\u5728\u86cb\u767d\u8cea\u7d44\u5b78\u5206\u6790\u4e4b\u524d\u901a\u904e\u751f\u7269\u7d20\u7094\u70f4\u6216\u6297\u9ad4\u7684\u514d\u75ab\u6c89\u6fb1\u9032\u884c\u5bcc\u96c6\u3002
\n\"\"<\/p>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK592-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Flow Cytometry (Ex\/Em 488\/(530\/590) nm) and Fluorescence Microscopy
\n\u2022 Sample type- Adherent and suspension cells
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications- This assay provides a convenient and accurate procedure to measure glycoproteins (Fucosylation) in biological samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFlow Cytometry (Ex\/Em 480\/(530\/590) nm) and Fluorescence Microscopy<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThis assay provides a convenient and accurate procedure to measure glycoproteins (Fucosylation) in biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nSimple, fast, does not require lengthy incubation times<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 EZClick\u2122 Wash Buffer (10X)
\n\u2022 Fixative Solution
\n\u2022 Permeabilization Buffer (10X)
\n\u2022 EZClick\u2122 GlcAz Label (1000X)
\n\u2022 Copper Reagent (100X)
\n\u2022 EZClick\u2122 Fluorescent Alkyne (100X)
\n\u2022 Reducing Agent (20X)
\n\u2022 EZClick\u2122 Total DNA Stain (1000X)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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