{"id":24946,"date":"2020-05-25T13:41:38","date_gmt":"2020-05-25T05:41:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24946"},"modified":"2023-11-20T15:49:17","modified_gmt":"2023-11-20T07:49:17","slug":"bpifb4-orf-vector-humanmouserat-porf-%e8%b2%a8%e8%99%9f1343101","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24946","title":{"rendered":"Bpifb4 ORF Vector | Bpifb4\u958b\u653e\u95b1\u8b80\u6846\u8868\u73fe\u8f09\u9ad4 \u8ca8\u865f1343101"},"content":{"rendered":"

Bpifb4 ORF Vector (<\/strong>Human,Mouse,Rat) (pORF) | <\/strong>\u8ca8\u865f<\/strong>1343101<\/a><\/span> (1.0 \u00b5g DNA)<\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846(Open Reading Frame, ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2( Lentivirus<\/a>)\u548cAAV<\/a>\u3001\u817a\u75c5\u6bd2( Adenovirus<\/a>\u00a0)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

<\/p>\n

Bpifb4 ORF Vector\u00a0\u8f09\u9ad4\u4ecb\u7d39: Bpifb4 ORF Vector\u00a0\u8f09\u9ad4\uff1bNM_182519<\/a>,NM_001034875<\/a>,NM_001109209<\/a>\u3002\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Aliases<\/strong><\/td>\nGm1006; Lplunc4; RP23-2L1.1<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNM_182519,NM_001034875,NM_001109209<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nNM_182519<\/a>,NM_001034875<\/a>,NM_001109209<\/a><\/td>\n<\/tr>\n
Gene Name<\/strong><\/td>\nBpifb4<\/td>\n<\/tr>\n
Full Gene Name<\/strong><\/td>\nLBP \/ BPI \/ CETP family, N-terminal domain containing protein; long palate, lung and nasal epithelium carcinoma-associated protein 4; ligand-binding protein RY2G5; Novel LBP\/BPI\/CETP family, N-terminal domain containing protein<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\nNM_001034875:1854
\nNM_001109209:1854<\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n2596bp<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nThis gene is available from: Human,Mouse,Rat<\/td>\n<\/tr>\n
List of bacterial markers<\/strong><\/td>\nSpectinomycin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

<\/p>\n\n\n\n\n\n\n
Vector Name<\/b><\/td>\npORF<\/td>\n<\/tr>\n
Antibiotic Information<\/b><\/td>\nBacterial: Spectinomycin<\/td>\n<\/tr>\n
Sequencing Primers<\/b><\/td>\nM13 FP: 5′-CCCAGTCACGACGTTGTAAAACG-3′
\nM13 RP: 5′-CAGGAAACAGCTATGAC-3′<\/td>\n<\/tr>\n
Additional Information<\/b><\/td>\nNo Additional Information<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Expression System Control Vector and Viruses<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
Lenti- backbones<\/td>\nBlank Control Lentivector<\/td>\nCat. No<\/td>\nBlank Control Virus<\/td>\nCat. No<\/td>\n<\/tr>\n
pLenti-GIII-CMV<\/a><\/td>\npLenti-III-Blank<\/a><\/td>\nLV587<\/td>\nLenti-III-Blank Lentivirus<\/a><\/td>\nLVP687<\/td>\n<\/tr>\n
pLenti-GIII-CMV-C-term-HA<\/a><\/td>\npLenti-III-Blank<\/a><\/td>\nLV587<\/td>\nLenti-III-Blank Lentivirus<\/a><\/td>\nLVP687<\/td>\n<\/tr>\n
pLenti-GIII-CMV-GFP-2A-Puro<\/a><\/td>\npLenti-CMV-GFP-2A-Puro-Blank Vector<\/a><\/td>\nLV590<\/td>\nLenti-CMV-GFP-2A-Puro-Blank Lentivirus<\/a><\/td>\nLVP690<\/td>\n<\/tr>\n
pLenti-GIII-CMV-RFP-2A-Puro<\/a><\/td>\npLenti-CMV-RFP-2A-Puro-Blank Vector<\/a><\/td>\nLV591<\/td>\nLenti-CMV-RFP-2A-Puro-Blank Lentivirus<\/a><\/td>\nLVP691<\/td>\n<\/tr>\n
pLenti-GIII-EF1a<\/a><\/td>\npLenti-EF1a-Blank Vector<\/a><\/td>\nLV588<\/td>\nLenti-EF1a-Blank Lentivirus<\/a><\/td>\nLVP688<\/td>\n<\/tr>\n
pLenti-GIII-UbC<\/a><\/td>\npLenti-UbC-Blank Vector<\/a><\/td>\nLV589<\/td>\nLenti-UbC-Blank Lentivirus<\/a><\/td>\nLVP689<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Expression System Control Vector and Viruses<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n
Other Reporter Control Lentivectors<\/td>\nCat. No<\/td>\n<\/tr>\n
Blank Promoter-Reporter Lentiviral Vector (GFP)<\/a><\/td>\nLV654<\/td>\n<\/tr>\n
\u00a0 Blank Promoter-Reporter Lentiviral Vector (Luc)<\/a><\/td>\nLV653<\/td>\n<\/tr>\n
\u00a0 CMV Promoter-Reporter Lentiviral Vector (GFP)<\/a><\/td>\nLV656<\/td>\n<\/tr>\n
\u00a0 CMV Promoter-Reporter Lentiviral Vector (Luc)<\/a><\/td>\nLV655<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Expression System Control Vector and Viruses<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
AAV- Backbones<\/td>\nBlank Control AAV<\/td>\nCat. No<\/td>\nSearch Viruses by Serotype<\/td>\n<\/tr>\n
pAAV-G-CMV-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (Amp)<\/a><\/td>\nAAV001a<\/td>\nAAV Blank Control Virus (CMV) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CMV-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (GFP) (Amp)<\/a><\/td>\nAAV003a<\/td>\nAAV Blank Control Virus (CMV) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CMV-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (Luc) (Amp)<\/a><\/td>\nAAV002a<\/td>\nAAV Blank Control Virus (CMV) (Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CMV-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CMV) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV016a<\/td>\nAAV Blank Control Virus (CMV) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (Amp)<\/a><\/td>\nAAV007a<\/td>\nAAV Blank Control Virus (EF1a) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (GFP) (Amp)<\/a><\/td>\nAAV009a<\/td>\nAAV Blank Control Virus (EF1a) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (Luc) (Amp)<\/a><\/td>\nAAV008a<\/td>\nAAV Blank Control Virus (EF1a) (Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-EF1a-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (EF1a) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV018a<\/td>\nAAV Blank Control Virus (EF1a) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (Amp)<\/a><\/td>\nAAV022a<\/td>\nAAV Blank Control Virus (hSync) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (SV40-GFP) (Amp)<\/a><\/td>\nAAV024a<\/td>\nAAV Blank Control Virus (hSync) (SV40-GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (SV40-Luc) (Amp)<\/a><\/td>\nAAV023a<\/td>\nAAV Blank Control Virus (hSync) (SV40-Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-hSyn-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (hSync) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV025a<\/td>\nAAV Blank Control Virus (hSync) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (Amp)<\/a><\/td>\nAAV004a<\/td>\nAAV Blank Control Virus (PGK) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (GFP) (Amp)<\/a><\/td>\nAAV006a<\/td>\nAAV Blank Control Virus (PGK) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-SV40-Luc-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (Luc) (Amp)<\/a><\/td>\nAAV005a<\/td>\nAAV Blank Control Virus (PGK) (Luc) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-PGK-CBH-gcGFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (PGK) (CBH-gcGFP) (Amp)<\/a><\/td>\nAAV020a<\/td>\nAAV Blank Control Virus (PGK) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CAGGS-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CAGGS) (Amp)<\/a><\/td>\nAAV013a<\/td>\nAAV Blank Control Virus (CAGGS) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CAGGS-SV40-GFP-hGH-amp<\/a><\/td>\nAAV Blank Control Vector (CAGGS) (GFP) (Amp)<\/a><\/td>\nAAV014a<\/td>\nAAV Blank Control Virus (CAGGS) (GFP) (Amp)<\/a><\/td>\n<\/tr>\n
pAAV-G-CAGGS-CBH-gcGFP Amp<\/a><\/td>\n\u00a0 AAV Blank Control Vector (CAGGS) (SV40-GFP) (Amp)<\/a><\/td>\nAAV021a<\/td>\nAAV Blank Control Virus (CAGGS) (CBH-gcGFP) (Amp)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Bpifb4 ORF Vector (Human,Mouse,Rat) (pORF) | \u8ca8\u865f1343101 […]<\/p>\n","protected":false},"author":3,"featured_media":29552,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5373,3],"tags":[1527],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24946"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24946"}],"version-history":[{"count":6,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24946\/revisions"}],"predecessor-version":[{"id":47207,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24946\/revisions\/47207"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29552"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24946"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24946"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24946"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}