{"id":24943,"date":"2020-05-25T12:34:52","date_gmt":"2020-05-25T04:34:52","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24943"},"modified":"2022-07-19T17:03:19","modified_gmt":"2022-07-19T09:03:19","slug":"ugt2b7-orf-humanrat-porf-%e8%b2%a8%e8%99%9f4914601","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24943","title":{"rendered":"UGT2B7 ORF vector | UGT2B7\u958b\u653e\u95b1\u8b80\u6846\u8868\u73fe\u8f09\u9ad4 \u8ca8\u865f4914601"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31\u00a0\u00a0<\/a><\/span><\/strong>UGT2B7 ORF (<\/strong>Human,Rat) (pORF) | <\/strong>\u8ca8\u865f<\/strong>4914601 (1.0 \u00b5g DNA)<\/a><\/span><\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846\uff08ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a><\/span>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a><\/span>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2(\u00a0lentivirus<\/a><\/span>)\u548c\u817a\u75c5\u6bd2(\u00a0adenovirus\u00a0<\/a><\/span>)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

UGT2B7 ORF<\/strong>\u8f09\u9ad4\u4ecb\u7d39: UGT2B7 ORF\u8f09\u9ad4\uff1b Accession Numbe BC030974,NM_173323<\/strong>\u3002\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Aliases<\/strong><\/td>\nUGT2B9<\/td>\n<\/tr>\n
HGNC ID<\/strong><\/td>\nHGNC:12554<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nBC030974,NM_173323<\/td>\n<\/tr>\n
Accession Number<\/strong><\/td>\nBC030974<\/a>,NM_173323<\/a><\/td>\n<\/tr>\n
Gene Name<\/strong><\/td>\nUGT2B7<\/td>\n<\/tr>\n
Full Gene Name<\/strong><\/td>\nUDP glucuronosyltransferase 2 family, polypeptide B7<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nClear Liquid<\/td>\n<\/tr>\n
Insert Size<\/strong><\/td>\nBC030974:1590
\nNM_173323:1593<\/td>\n<\/tr>\n
Vector Size<\/strong><\/td>\n2596bp<\/td>\n<\/tr>\n
Storage Buffer<\/strong><\/td>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Species<\/strong><\/td>\nThis gene is available from: Human,Rat<\/td>\n<\/tr>\n
List of bacterial markers<\/strong><\/td>\nSpectinomycin<\/td>\n<\/tr>\n
Guarantee<\/strong><\/td>\nabm guarantees that the correct ORF construct is provided. If this is not the case, we will provide a one-time replacement. The replacement will not be covered by the same guarantee.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1
\nLentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/span><\/a> | Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316) <\/a>\u00a0| Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a> | Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/span><\/p>\n

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\u7522\u54c1\u540d\u7a31\u00a0\u00a0UGT2B7 ORF (Human,Rat) (pORF) | \u8ca8\u865f4914601 (1.0 \u00b5g […]<\/p>\n","protected":false},"author":3,"featured_media":24012,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1528],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24943"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24943"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24943\/revisions"}],"predecessor-version":[{"id":24951,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24943\/revisions\/24951"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24012"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24943"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24943"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}