{"id":24934,"date":"2020-05-23T22:46:47","date_gmt":"2020-05-23T14:46:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24934"},"modified":"2022-07-19T17:03:28","modified_gmt":"2022-07-19T09:03:28","slug":"goat-anti-type-iii-collagen-unlb%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f1320-01","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24934","title":{"rendered":"Goat Anti-Type III Collagen-UNLB\u6297\u9ad4 | \u8ca8\u865f1330-01"},"content":{"rendered":"

Goat Anti-Type III Collagen-UNLB\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>1330-01<\/a><\/span> \u00a0<\/strong>\u898f\u683c<\/strong>200ug \u00a0(0.4 mg\/mL)<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e00\u7d1a\u6297\u9ad4\u53ca\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0\u5c71\u7f8a\u6297III\u578b\u81a0\u539f\u86cb\u767d-UNLB\u6297\u9ad4\uff0c\u4e0d\u5177\u6a19\u8a8c\u3002\u91dd\u5c0dtype III collagen\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baPooled antisera from goats hyperimmunized with type III collagen\u3002Isotype\u70baGoat IgG\u3002Isotype Control Goat IgG-UNLB<\/a>\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u5728Borate buffered saline, pH 8.2\u3002<\/p>\n

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Paraffin embedded human gastric cancer tissue was stained with Goat IgG-UNLB isotype control (SB Cat. No. 0109-01; left) and Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01; right) followed by Swine Anti-Goat IgG(H+L), Human\/Rat\/Mouse SP ads-HRP (SB Cat. No. 6300-05), DAB, and hematoxylin.<\/p>\n

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Frozen osteopontin-null mouse muscle section was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody.<\/div>\n
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Paraplast Plus\u00ae embedded 2K1C rat kidney section was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody and DAB and hematoxylin.<\/p>\n

Image from Cuevas CA, Tapia-Rojas C, Cespedes C, Inestrosa NC, Vio CP. \u03b2-catenin-dependent signaling pathway contributes to renal fibrosis in hypertensive rats. Biomed Res Int. 2015;2015:726012. Figure 2(e)<\/p>\n

Paraplast Plus\u00ae is a registered trademark of Leica Biosystems Richmond, Inc.<\/p>\n<\/div>\n<\/div>\n

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Frozen kidney section following unilateral ureteral obstruction was stained with Goat Anti-Type III Collagen-UNLB (SB Cat. No. 1330-01) followed by a secondary antibody.<\/p>\n

Image from Maeshima A, Mishima K, Yamashita S, Nakasatomi M, Miya M, Sakurai T, et al. Follistatin, an activin antagonist, ameliorates renal interstitial fibrosis in a rat model of unilateral ureteral obstruction. Biomed Res Int. 2014;2014:376191. Figure 5(a)<\/p>\n

Image from Gawlik KI, Holmberg J, Svensson M, Einerborg M, Oliveira BM, Deierborg T. Potent pro-inflammatory and pro-fibrotic molecules, osteopontin and galectin-3, are not major disease modulators of laminin \u03b12 chain-deficient muscular dystrophy. Sci Rep. 2017;10:1038. Figure 6(a)<\/p>\n<\/div>\n

<\/div>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Specificity<\/strong><\/td>\nReacts with conformational determinants on type III collagen<\/td>\n<\/tr>\n
Source<\/strong><\/td>\nPooled antisera from goats hyperimmunized with type III collagen<\/td>\n<\/tr>\n
Cross Adsorption<\/strong><\/td>\nCollagen types I, II, IV, V, and VI<\/td>\n<\/tr>\n
Purification<\/strong><\/td>\nAffinity chromatography on type III collagen covalently linked to agarose<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nGoat IgG<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nGoat IgG-UNLB<\/a><\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nUNLB (Unconjugated)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nBorate buffered saline, pH 8.2<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.4 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n0.5 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C Please refer to product specific SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Quality tested applications for relevant formats include –<\/p>\n

ELISA 1-3<\/sup><\/p>\n

Other referenced applications for relevant formats include –<\/p>\n

Immunohistochemistry-Paraffin Sections 4-20<\/sup> Immunohistochemistry-Frozen Sections 6,9,12,15,21-31<\/sup> Immunocytochemistry 9,32,33<\/sup> Electron Microscopy 6,12,34,35<\/sup> Flow Cytometry 36,37<\/sup> Western Blot 4,10,13,14,22,38,39<\/sup><\/td>\n<\/tr>\n

Recommended Dilutions<\/strong><\/td>\nPlease refer to product specific Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2794734<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n
Gene ID<\/strong><\/td>\n1281<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
Gene Symbol<\/strong><\/td>\nCOL3A1<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
Gene Aliases<\/strong><\/td>\nEDS4A; EDSVASC; PMGEDSV<\/td>\n<\/tr>\n
Uniprot ID<\/strong><\/td>\nP02461<\/a>\u00a0(Human)<\/td>\n<\/tr>\n
Uniprot Name<\/strong><\/td>\nCO3A1_HUMAN<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/p>\n

References<\/p>\n

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10.Combet S, Ferrier M, Van Landschoot M, Stoenoiu M, Moulin P, Miyata T, et al. Chronic uremia induces permeability changes, increased nitric oxide synthase expression, and structural modifications in the peritoneum. J Am Soc Nephrol. 2001;12:2146-57. (IHC-PS, WB, Rat Reactivity)<\/p>\n

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 <\/p>\n","protected":false},"excerpt":{"rendered":"

Goat Anti-Type III Collagen-UNLB\u6297\u9ad4 | \u8ca8\u865f1330-01 \u00a0\u898f\u683c200ug […]<\/p>\n","protected":false},"author":3,"featured_media":18700,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136],"tags":[1529],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24934"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24934"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24934\/revisions"}],"predecessor-version":[{"id":24940,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24934\/revisions\/24940"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/18700"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24934"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24934"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24934"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}