{"id":24924,"date":"2020-05-23T16:06:32","date_gmt":"2020-05-23T08:06:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24924"},"modified":"2022-07-19T17:03:42","modified_gmt":"2022-07-19T09:03:42","slug":"%e6%8a%97covid-19-sars-cov-s%e7%b3%96%e8%9b%8b%e7%99%bd%e6%8a%97%e9%ab%94%e8%b2%a8%e8%99%9fab1680-23-0","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24924","title":{"rendered":"\u6297COVID-19 + SARS-CoV S\u7cd6\u86cb\u767d\u6297\u9ad4|\u8ca8\u865fab1680-23.0"},"content":{"rendered":"

Anti-COVID-19 & SARS-CoV S glycoprotein [CR3022], Rabbit IgG, Kappa\u6297\u9ad4 |\u8ca8\u865fAb01680-23.0<\/a> \u00a0\u00a0<\/strong><\/span><\/p>\n

COVID19 , S\u86cb\u767d;SARS-CoV S\u86cb\u767d;S\u7cd6\u86cb\u767d;E2; SARS\u51a0\u72c0\u75c5\u6bd2; SARS-CoV-2;SARS CoV 2;2019-nCoV<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> COVID19\u6297\u9ad4\u8207SARS-CoV\u548cSARS-CoV-2\u7d50\u5408,\u5177\u6709\u9ad8\u89aa\u548c\u529b(PMID:16796401 = 32065055)\u3002\u8a72\u6297\u9ad4\u7d50\u5408\u7684\u521d\u59cb\u7279\u5fb5\u7531ELISA\u9032\u884c,\u8868\u660e\u8a3a\u65b7\u6aa2\u6e2c\u7684\u767c\u5c55\u6f5b\u529b,\u7121\u8ad6\u662f\u75c5\u6bd2\u6355\u7372\u6aa2\u6e2c,\u9084\u662f\u4f5c\u70ba\u8840\u6e05\u5b78\u6e2c\u5b9a\u4e2d\u6e2c\u91cf\u75c5\u6bd2\u66b4\u9732\u514d\u75ab\u53cd\u61c9\u7684\u63a7\u5236\u3002\u4eba\u985eIgG1\u3001IgG3\u3001IgM\u548cIgA\u7b49\u503c\u578b\u53ef\u7528\u65bc\u985e\u6bd4COVID19(Amanat\u7b49\u4eba,2020\u5e74)\u4e2d\u770b\u5230\u7684\u6297\u9ad4\u53cd\u61c9\u3002\u4eba\u985eIgG2\u548cIgG4\u4e9e\u578b,\u4e5f\u51fa\u73fe\u5728COVID-19\u60a3\u8005\u7684\u4e00\u5c0f\u90e8\u5206,\u4e5f\u53ef\u4ee5\u8abf\u67e5\u4ed6\u5011\u5728\u61c9\u5c0dSARS-CoV-2\u4e2d\u7684\u4f5c\u7528\u3002\u6700\u521d\u7684\u4eba\u985eIgG1\u7248\u672c\u7684\u6297\u9ad4\u8207\u53e6\u4e00\u7a2e\u975e\u6297\u6027SARS\u6297\u9ad4CR3014\u5354\u540c\u5de5\u4f5c,\u662fSARS-CoV\u611f\u67d3\u88ab\u52d5\u514d\u75ab\u9810\u9632\u7684\u6f5b\u5728\u5019\u9078\u8005(Meulen\u7b49\u4eba,2006\u5e74)\u3002\u64da\u5831\u5c0e,\u539f\u59cb\u6297\u9ad4(\u4eba\u985eIgG1)\u9084\u7d50\u54082019-nCoV RBD(KD\u70ba6.3 nM)\u3002\u9019\u7a2e\u6297\u9ad4\u88ab\u6b78\u7d50\u70ba\u4e00\u7a2e\u4f5c\u70ba\u6cbb\u7642\u5291,\u55ae\u7368\u6216\u8207\u5176\u4ed6\u4e2d\u548c\u6297\u9ad4\u806f\u5408\u7528\u65bc\u6cbb\u76422019-nCoV\u611f\u67d3\u7684\u6f5b\u529b(Tian\u7b49\u4eba,2020\u5e74)\u3002<\/p>\n

P<\/strong>urification:<\/strong> Protein A affinity purified<\/p>\n

Supplied in:<\/strong> PBS with 0.02% Proclin 300.<\/p>\n

Storage Recommendation:<\/strong> Store at 4\u2070C for up to 3 months. For longer storage, aliquot and store at -20\u2070C.<\/p>\n

Concentration:<\/strong> 1 mg\/ml.<\/p>\n

\u514d\u75ab\u539f:\u539f\u59cb\u55ae\u514b\u9686\u6297\u9ad4\u662f\u901a\u904e\u5c0d\u66b4\u9732\u65bcSARS-CoV\u7684\u60a3\u8005\u5916\u5468\u8840\u6dcb\u5df4\u7d30\u80de\u9032\u884c\u6e2c\u5e8f\u7522\u751f\u7684\u3002\u7279\u7570\u6027:\u9019\u7a2e\u6297\u9ad4\u5c07SARS-CoV\u5c16\u5cf0\u86cb\u767d\u7684S1\u57df\u4e2d\u7684\u6c28\u57fa\u9178318-510\u8207SARS-CoV-2(COVID-19)\u5c16\u5cf0\u86cb\u767d\u7d50\u5408\u3002\u6297\u9ad4\u9084\u8207P462L\u66ff\u4ee3S318-510\u7247\u6bb5\u7684SARS\u5c16\u5cf0\u86cb\u767d\u7d50\u5408\u3002\u7d50\u5408\u7684\u8868\u4e0a\u76ae\u53ea\u80fd\u5728\u5c16\u5cf0\u86cb\u767d\u7684\u300c\u958b\u653e\u300d\u532f\u7d50\u4e2d\u7372\u5f97(Joyce\u7b49\u4eba,2020\u5e74)<\/p>\n

Antibody first published in:<\/strong> ter Meulen et al. Human Monoclonal Antibody Combination against SARS Coronavirus: Synergy and Coverage of Escape Mutants PLoS Med. 2006 Jul; 3(7): e237 PMID:16796401<\/a>
\nNote on publication:<\/strong> Describes the discovery and original characterization of this antibody.<\/p>\n

\"Binding<\/p>\n

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (Ab01680-10.0) to SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag and SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag from HEK293 cells. <\/b> ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), Sheep Fc-Tag (blue line) or SARS-CoV-2 Spike Glycoprotein (S2), Sheep Fc-Tag (orange line) from HEK293 cells (The Native Antigen Company) at concentrations of 5 \u00b5g\/ml. A 3-fold serial dilution from 125 ng\/ml was performed using Ab01680-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.<\/p>\n

\"Binding<\/p>\n

Binding curve of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (Ab01680-10.0) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 of various origin. <\/b> ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line), SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) and SARS Coronavirus Spike Glycoprotein (S1), His-Tag (HEK293 cells; blue line) (The Native Antigen Company) at concentrations of 5 \u00b5g\/ml. A 3-fold serial dilution from 41.6 ng\/ml was performed using Ab01680-10.0. For detection, a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.<\/p>\n

\"Binding<\/p>\n

Binding curve of four different formats of anti-COVID-19 & SARS-CoV S glycoprotein antibody CR3022 (Ab01680) to SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)). <\/b> ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) (Native Antigen) at concentrations of 5 \u00b5g\/ml. A 3-fold serial dilution from 41.6 ng\/ml was performed using Ab01680-10.0; from 370 ng\/ml for Ab01680-16.0 and from 10000 ng\/ml for Ab01680-15.0 and Ab01680-11.0. Human IgM, human IgA and human IgG2 were HRP-conjugated and for the detection of human IgG1 a 1:4000 dilution of HRP-labelled anti-human IgG antibody was used.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\nMouse target<\/td>\n\u6297\u9ad4clone<\/td>\nOriginal Antibody format<\/td>\n<\/tr>\n
Ab01052<\/a><\/td>\n4-1BB (CD137)<\/td>\nLOB12.3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n<\/thead>\n
Ab01054<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nAT113-2<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00881<\/a><\/td>\n4-1BBL (CD137L)<\/td>\nTKS-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01027<\/a><\/td>\nBTLA<\/td>\nHMBT-6B2 <\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00670<\/a><\/td>\nCD27<\/td>\nLG.3A10<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00895<\/a><\/td>\nCD27<\/td>\nRM27-3E5 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00286<\/a><\/td>\nCD28<\/td>\nE18 <\/a><\/td>\nMouse IgG2b<\/td>\n<\/tr>\n
Ab00285<\/a><\/td>\nCD28<\/td>\nD665 <\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01109<\/a><\/td>\nCD40<\/td>\n3\/23 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01087<\/a><\/td>\nCD40L (CD154)<\/td>\nMR1 <\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00816<\/a><\/td>\nCD70<\/td>\nTAN 1-7 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01056<\/a><\/td>\nCD70<\/td>\nTAN1-6 <\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01028<\/td>\nCD80 (B7-1)<\/td>\nRM80<\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01029<\/a><\/td>\nCD86 (B7-2)<\/td>\nPO.3<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01089<\/a><\/td>\nCD153<\/td>\nRM153<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab01297<\/a><\/td>\nCD96<\/td>\n6A6<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00894<\/a><\/td>\nCTLA-4<\/td>\n9H10<\/a><\/td>\nHamster IgG2<\/td>\n<\/tr>\n
Ab01018<\/a><\/td>\nCTLA-4<\/td>\n9D9<\/a><\/td>\nMouse IgG2b<\/td>\n<\/tr>\n
Ab01088<\/a><\/td>\nGAL-9<\/td>\nRG9-35<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01060<\/a><\/td>\nGITR<\/td>\nDTA-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00374<\/a><\/td>\nGITR<\/td>\nYGITR 860.103.5<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00631<\/a><\/td>\nGITR<\/td>\nYGITR765<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00630<\/a><\/td>\nGITRL<\/td>\nYGL386<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01026<\/a><\/td>\nHVEM<\/td>\nHMHV-1B18<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00814<\/a><\/td>\nICOS<\/td>\n7E.17G9<\/a><\/td>\nRat IgG2b<\/td>\n<\/tr>\n
Ab00882<\/a><\/td>\nICOSL<\/td>\nHK5.3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01295<\/a><\/td>\nLAG3<\/td>\nC9B7W<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00110<\/a><\/td>\nOX40 (CD134)<\/td>\nOX86<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00564<\/a><\/td>\nOX40L<\/td>\nOX-89<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab00813<\/a><\/td>\nPD-1<\/td>\nRMP1-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01017<\/a><\/td>\nPD-1H (VISTA)<\/td>\nmam82<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01016<\/a><\/td>\nPD-1H (VISTA)<\/td>\nMH5A<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab00373<\/a><\/td>\nPD-L1<\/td>\nYDC 127.1.1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00883<\/a><\/td>\nPD-L2<\/td>\nTY25<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00875<\/a><\/td>\nPDPN (MAP-Tag)<\/td>\nPMab-1<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab00973<\/a><\/td>\nTIGIT<\/td>\n4D4<\/a><\/td>\nHamster IgG<\/td>\n<\/tr>\n
Ab01258<\/a><\/td>\nTIGIT<\/td>\n1B4<\/a><\/td>\nMouse IgG1<\/td>\n<\/tr>\n
Ab01032<\/a><\/td>\nTIM-1<\/td>\n3B3<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01110<\/a><\/td>\nTIM-2<\/td>\nRMT2-14<\/a><\/td>\nRat IgG2a<\/td>\n<\/tr>\n
Ab01057<\/a><\/td>\nTIM-3<\/td>\n2C12<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01058<\/a><\/td>\nTIM-4<\/td>\n5G3<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
Ab01059<\/a><\/td>\nTIM-4<\/td>\n3A1<\/a><\/td>\nRat IgG1<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Anti-COVID-19 & SARS-CoV S glycoprotein [CR3022], R […]<\/p>\n","protected":false},"author":3,"featured_media":3519,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1531],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24924"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24924"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24924\/revisions"}],"predecessor-version":[{"id":25179,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24924\/revisions\/25179"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3519"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24924"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24924"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24924"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}