{"id":24914,"date":"2020-05-22T14:40:59","date_gmt":"2020-05-22T06:40:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24914"},"modified":"2022-07-19T17:03:51","modified_gmt":"2022-07-19T09:03:51","slug":"qpcr%e8%85%ba%e7%97%85%e6%af%92%e5%ae%9a%e9%87%8f-qpcr-adeno-associated-virus-titration-titer-kit%e8%b2%a8%e8%99%9fg931","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24914","title":{"rendered":"qPCR\u817a\u75c5\u6bd2\u5b9a\u91cf | qPCR Adeno-Associated Virus Titration (Titer) Kit\u8ca8\u865fG931"},"content":{"rendered":"

qPCR Adeno-Associated Virus Titration (Titer) Kit\u00a0<\/strong>\u75c5\u6bd2\u5feb\u901f\u5b9a\u91cf\u5957\u7d44<\/strong>\"\"<\/p>\n

qPCR Adeno-Associated Virus Titration (Titer) Kit-G931<\/a><\/span><\/p>\n

qPCR\u817a\u75c5\u6bd2\u76f8\u95dc\u5b9a\u91cf\u8a66\u5291\u76d2\uff08qPCR Adeno-Associated Virus Titration, G931\uff09\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u7c21\u55ae\u7684\u65b9\u6cd5\u4f86\u9032\u884cqPCR\u817a\u75c5\u6bd2\u5b9a\u91cf\u3002\u8a72\u8a66\u5291\u76d2\u63a1\u7528\u5feb\u901f\u7684DNA\u63d0\u53d6\u6b65\u9a5f\uff0c\u4e26\u4f7f\u7528\u53ef\u57282\u5c0f\u6642\u5167\u5b8c\u6210\u7684qPCR\u6e2c\u5b9a\u6cd5\u78ba\u5b9a\u75c5\u6bd2DNA\u529b\u50f9\u3002\u8207\u5e02\u5834\u4e0a\u7684\u540c\u985e\u7522\u54c1\u76f8\u6bd4\uff0cabms\u8a66\u5291\u76d2\u5df2\u8b49\u660e\u81ea\u5df1\u5728\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\u4e0a\u5747\u512a\u8d8a\uff0c\u540c\u6642\u5c07\u975e\u7279\u7570\u6027\u80cc\u666f\u4fe1\u865f\u6e1b\u81f3\u6700\u5c11\u3002\u4f7f\u7528\u63d0\u4f9b\u7684\u75c5\u6bd2\u88c2\u89e3\u7de9\u885d\u6db2\u5f9e\u4e00\u5c0f\u4efd\u672a\u77e5\u6a23\u54c1\u4e2d\u5206\u96e2\u75c5\u6bd2DNA\u3002\u7136\u5f8c\u5c07\u5206\u96e2\u7684DNA\u8207\u5169\u7a2e\u5df2\u77e5\u7684AAV\u6a19\u6e96\u4e00\u8d77\u7d0d\u5165qPCR\u5206\u6790\u4e2d\uff0c\u4ee5\u78ba\u5b9a\u95be\u503c\u5faa\u74b0\uff08Ct\uff09\u503c\u3002\u7136\u5f8c\u53ef\u4ee5\u4f7f\u7528\u6240\u5f97Ct\u503c\u901a\u904eAAV\u6ef4\u5b9a\u5ea6\u8a08\u7b97\u78ba\u5b9a\u672a\u77e5\u6a23\u54c1\u7684\u6700\u7d42\u75c5\u6bd2\u529b\u50f9\u3002 \u6a23\u54c1\u88fd\u5099: \u5c0d\u65bc\u76f4\u63a5\u4f86\u81ea\u7522\u75c5\u6bd2\u7d30\u80de\u7cfb\u7684\u75c5\u6bd2\u6a23\u54c1\uff1a\u6536\u96c6\u57f9\u990a\u57fa\u4e26\u4ee52000g\u96e2\u5fc35\u5206\u9418\u4ee5\u53bb\u9664\u7d30\u80de\/\u788e\u7247\u3002\u5728\u9032\u884c\u75c5\u6bd2DNA\u5206\u96e2\u6b65\u9a5f\u4e4b\u524dDNase I Treat\u3002\u5c0d\u65bc\u7d14\u5316\u7684\u75c5\u6bd2\u6a23\u54c1\uff1a\u75281X PBS\u5c07\u75c5\u6bd2\u7a00\u91cb\u81f3108-9<\/sup> GC \/ ml\u4e26\u76f4\u63a5\u9032\u884c\u75c5\u6bd2DNA\u5206\u96e2\u6b65\u9a5f\uff08\u4e0d\u9700\u8981DNase I\u8655\u7406\uff09\u3002<\/p>\n

abm \u7684 qPCR AAV Titer Kit \u662f\u4e00\u7a2e\u4e00\u6b65\u6cd5\u6aa2\u6e2c\uff0c\u63a1\u7528\u5feb\u901f\u88c2\u89e3\u6b65\u9a5f\uff0c\u7136\u5f8c\u662f qPCR\u3002\u8a72\u8a66\u5291\u76d2\u65e8\u5728\u63d0\u4f9b\u9ad8\u9748\u654f\u5ea6\u548c\u7279\u7570\u6027\uff0c\u8207\u985e\u4f3c\u8a66\u5291\u76d2\u76f8\u6bd4\uff0c\u53ef\u78ba\u4fdd\u6700\u5c0f\u7684\u975e\u7279\u7570\u6027\u80cc\u666f\u548c\u66f4\u597d\u7684\u6574\u9ad4\u6027\u80fd\u3002 ROX \u53c3\u8003\u67d3\u6599\u8207 MasterMix \u5206\u958b\u63d0\u4f9b\uff0c\u4f7f\u8a72\u8a66\u5291\u76d2\u8207\u5927\u591a\u6578 qPCR \u5100\u5668\u666e\u904d\u517c\u5bb9\u3002 MasterMix \u542b\u6709\u8207 SYBR Green\u2122 \u548c EvaGreen\u2122 \u76f8\u7576\u7684\u67d3\u6599\u3002<\/p>\n

    \n
  1. 2\u5c0f\u6642\u5167\u5feb\u901f\u7d14\u5316\u75c5\u6bd2\u3002<\/li>\n
  2. \u53ef\u9054\u6fc3\u7e2e\u6548\u679c\u7d04100x\u3002<\/li>\n<\/ol>\n\n\n\n\n\n\n\n\n\n\n\n
    PRODUCT COMPONENT<\/strong><\/th>\nQUANTITY<\/strong><\/th>\n<\/tr>\n<\/thead>\n
    BlasTaq\u2122 2X qPCR Titer MasterMix<\/td>\n1.25 ml<\/td>\n<\/tr>\n
    Primer Mix<\/td>\n100 rxn (200 \u00b5l)<\/td>\n<\/tr>\n
    Virus Lysis Buffer<\/td>\n800 \u00b5l<\/td>\n<\/tr>\n
    Standard Control DNA<\/td>\n50 \u00b5l<\/td>\n<\/tr>\n
    DNase I Reaction Mix<\/td>\n2 x 1.0 ml<\/td>\n<\/tr>\n
    ROX Reference Dye<\/td>\n15 \u00b5l<\/td>\n<\/tr>\n
    Nuclease-Free H2<\/sub>O<\/td>\n2 x 1.0 ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u53e6\u63d0\u4f9bABM qPCR Lentivirus Titration (Titer) Kit<\/strong><\/p>\n

      \n
    1. One-Step qPCR\uff0c2\u5c0f\u6642\u5167\u53ef\u5b8c\u6210\u5b9a\u91cf\u3002<\/li>\n
    2. Primer\u7d93\u7279\u6b8a\u8a2d\u8a08\uff0c\u89e3\u6c7aprimer dimer\u5e72\u64fe\u3002<\/li>\n
    3. \u9644\u6709\u75c5\u6bd2\u6fc3\u5ea6\u6a19\u6e96\u6db2\u5169\u7d44\uff0c\u6e96\u78ba\u5b9a\u91cf\u75c5\u6bd2\u6fc3\u5ea6\u3002<\/li>\n
    4. \u642d\u914dABM qPCR Mastermix\uff0c\u6548\u679c\u66f4\u4f73\u3002<\/li>\n<\/ol>\n

      \u200bData Analysis The titer of your sample(s) can be calculated from Ct values by using our on-line AAV titer calculator at https:\/\/www.abmgood.com\/AAV-Titration-Titer-Kits.html<\/a>. \u00a0Alternatively, you could calculate the titer according to the following formula: Titer of viral lysate = 5 x 109 \/23(Ctx-Ct1)\/(Ct2-Ct1) Ctx = Average of 3 Ct values of the unknown sample Ct1 = Average of 3 Ct values of STD1 Ct2 = Average of 3 Ct values of STD2<\/p>\n

       <\/p>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      qPCR Adeno-Associated Virus Titration (Titer) Kit\u00a0\u75c5\u6bd2\u5feb\u901f\u5b9a […]<\/p>\n","protected":false},"author":3,"featured_media":24915,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1532],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24914"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24914"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24914\/revisions"}],"predecessor-version":[{"id":32096,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24914\/revisions\/32096"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24915"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24914"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24914"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24914"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}