{"id":24708,"date":"2020-05-13T13:09:04","date_gmt":"2020-05-13T05:09:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24708"},"modified":"2022-07-19T17:15:00","modified_gmt":"2022-07-19T09:15:00","slug":"mouse-anti-chicken-mhc-class-ii-pe%e6%8a%97%e9%ab%94-%e8%b2%a8%e8%99%9f8350-09","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24708","title":{"rendered":"Mouse Anti-Chicken MHC Class II-PE\u6297\u9ad4 | \u8ca8\u865f8350-09"},"content":{"rendered":"

Mouse Anti-Chicken MHC Class II-PE\u6297\u9ad4 | <\/strong>\u8ca8\u865f<\/strong><\/a><\/span>8<\/a>350-09<\/span> <\/strong>\u898f\u683c<\/strong>1.0 mg \u00a0(1.0 mg\/mL)<\/p>\n

\u7f8e\u570b\u6297\u9ad4\u751f\u7522\u5546Southern Biotech\u65bc1982\u5e74\uff0c\u7531UAB\u7d30\u80de\u514d\u75ab\u5c08\u5bb6Dr. Max Cooper\u8207\u5be6\u9a57\u5ba4\u6210\u54e1\u5171\u540c\u5275\u7acb\u3002SouthernBiotech\u81f4\u529b\u65bc\u751f\u7522\u9ad8\u54c1\u8cea\u4e00\u7d1a\u53ca\u4e8c\u7d1a\u6297\u9ad4\uff0c\u76ee\u524d\u9664\u4e86\u4e00\u822c\u5e38\u898b\u7684\u591a\u682a\u8207\u55ae\u682a\u4e8c\u7d1a\u6297\u9ad4\u5916\uff0c\u9084\u5e36\u6709\u87a2\u5149\u7269\u8cea\uff08FITC\u3001Spectral Red\u3001Alexa 488\u3001Alexa 647\u3001Pacific Blue\uff09\u3001\u9175\u7d20\uff08HRP\u3001AP\uff09\u3001Low Endotoxin\/Azide Free\uff08LE\/AF\uff09\u7b49\u6a19\u5b9a\uff0c\u4ee5\u53caF(ab)\u548cF(ab\u2019)2\u7b49\u6297\u9ad4\u7247\u6bb5\u3002\u8af8\u5982\u6b64\u985e\u591a\u6a23\u5316\u7522\u54c1\uff0c\u5354\u52a9\u5404\u7a2e\u5be6\u9a57\u9032\u884c\u3002<\/p>\n

\u96de\u4e3b\u8981\u7d44\u7e54\u76f8\u5bb9\u6027\u8907\u5408\u7269(chicken major histocompatibility complex , MHC),\u6216B\u8907\u5408\u9ad4(B complex),\u7531\u5e7e\u500b\u9ad8\u5ea6\u591a\u614b\u57fa\u56e0\u7c07(highly polymorphic genes)\u7d44\u6210\u3002\u8207\u54fa\u4e73\u52d5\u7269\u4e00\u6a23,\u9ce5\u985eMHC\u5c0d\u514d\u75ab\u53cd\u61c9(immune response)\u3001\u6297\u75c5(disease resistance)\u3001\u751f\u9577\u9aee\u80b2(growth and development)\u3001\u8870\u8001(aging)\u548c\u7e41\u6b96(reproduction)\u7b49\u5404\u7a2e\u91cd\u8981\u7684\u751f\u7269\u529f\u80fd\u5177\u6709\u907a\u50b3\u5f71\u97ff\u3002\u96deMHC II\u985e\u57fa\u56e0,\u4e5f\u7a31\u70baB-L\u5206\u5340\u57df(B-L subregion),\u96deMHC\u7de8\u78bc\u7d30\u80de\u8868\u9762\u7cd6\u86cb\u767d(chicken MHC encode cell surface glycoproteins)\u662f\u54fa\u4e73\u52d5\u7269II\u985e\u6297\u539f\u7684\u540c\u6e90\u3002B-L\u6297\u539f\u5728\u7d50\u69cb\u4e0a\u8207\u54fa\u4e73\u52d5\u7269II\u985e\u5206\u5b50\u76f8\u4f3c,\u56e0\u70ba\u5b83\u5011\u662f\u4e00\u500b\u91cd\u93c8\u548c\u4e00\u689d\u8f15\u93c8\u7684\u975e\u5171\u50f9\u7d50\u5408\u7684\u6697\u89d2\u3002MHC II \u985e\u4e3b\u8981\u8868\u73fe\u5728 B \u7d30\u80de\u548c\u6297\u539f\u5448\u73fe\u7d30\u80de (antigen presenting cells , APC)\u3002<\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> \u00a0Mouse Anti-Chicken MHC Class II-PE\u6297\u9ad4\uff0c\u5177PE (R-phycoerythrin)\u6a19\u8a8c\u3002\u91dd\u5c0dChicken\/Pigeon\/Caiman MHC class II \u03b2-chain\u5177\u7279\u7570\u6027\u3002\u4f86\u6e90\u70baSwine\u6297\u8840\u6e05\u3002Isotype\u70baSwine IgG\u3002Isotype Control Goat IgG-FITC<\/a>\u3002 \u7de9\u885d\u6db2\u914d\u65b9\u5728Phosphate buffered saline \u542b\u6709 0.1% sodium azide\u9632\u8150\u5291\u53castabilizer\u3002<\/p>\n

\"\"<\/p>\n

Chicken peripheral blood lymphocytes were stained with Mouse Anti-Chicken MHC Class II-PE (SB Cat. No. 8350-09) and Mouse Anti-Chicken CD3-FITC (SB Cat. No. 8200-02).<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Clone<\/strong><\/td>\n2G11<\/td>\n<\/tr>\n
Isotype<\/strong><\/td>\nMouse IgG1<\/sub>\u03ba<\/td>\n<\/tr>\n
Isotype Control<\/strong><\/td>\nMouse IgG1<\/sub>-PE<\/a><\/td>\n<\/tr>\n
Specificity<\/strong><\/td>\nChicken\/Pigeon\/Caiman MHC class II \u03b2-chain<\/td>\n<\/tr>\n
Alternalte Name(s)<\/strong><\/td>\nB-L<\/td>\n<\/tr>\n
Format\/Conjugate<\/strong><\/td>\nPE (R-phycoerythrin)<\/td>\n<\/tr>\n
Buffer Formulation<\/strong><\/td>\nPhosphate buffered saline containing 0.1% sodium azide and a stabilizer<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n0.1 mg\/mL<\/td>\n<\/tr>\n
Volume<\/strong><\/td>\n1.0 mL<\/td>\n<\/tr>\n
Storage & Handling<\/strong><\/td>\n2-8\u00b0C; Avoid exposure to light; Do not freeze Please refer to product specific SDS<\/a><\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\n

Applications for relevant formats of this clone include –<\/p>\n

Flow Cytometry \u2013 Quality tested 1,4,6-19<\/sup> Immunohistochemistry-Frozen Sections \u2013 Reported in literature 1,4,20<\/sup> Electron Microscopy \u2013 Reported in literature 5<\/sup> Immunoprecipitation \u2013 Reported in literature 1,2<\/sup> Purification \u2013 Reported in literature 3<\/sup><\/td>\n<\/tr>\n

Recommended\u00a0Dilutions<\/strong><\/td>\nPlease refer to product specific Technical Bulletin<\/a><\/td>\n<\/tr>\n
RRID<\/strong><\/td>\nAB_2796515<\/td>\n<\/tr>\n
\u00a0<\/strong><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n
Gene ID<\/strong><\/td>\n\n

693256<\/a>\u00a0(Chicken)<\/p>\n

101747454<\/a>\u00a0(Chicken)<\/td>\n<\/tr>\n

Gene Symbol<\/strong><\/td>\n\n

BLB1<\/a>\u00a0(Chicken)<\/p>\n

BLB2<\/a>\u00a0(Chicken)<\/td>\n<\/tr>\n

Gene Aliases<\/strong><\/td>\nB-LB; B-LB21; GSP-BLB1; B-L; B-LB2; B-LBII; GSP-BLB2; S19<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

SBA<\/strong>\u71b1\u92b7\u5546\u54c1<\/strong>\u00a0\u00a07-AAD\u7d30\u80de\u6d3b\u6027\u87a2\u5149\u6307\u793a\u5291<\/a>\u00a0|\u00a0monocloal\u00a0isotyping\u5957\u7d44<\/a>\u00a0|\u00a0Annexin V-FITC\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c<\/a><\/u>\u00a0|\u00a0<\/u>\u00a0DAPI\u87a2\u5149\u5c01\u7247\u5291\u00a0<\/a>\u00a0|<\/u>\u00a0<\/u>anti-rabbit\u00a0\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0<\/u>|\u00a0<\/u>anti-mouse\u4e8c\u7d1a\u6297\u9ad4<\/a>\u00a0| <\/u>\u81a0\u539f\u86cb\u767dCollagen Type I\u00a0\u00a0<\/a>| \u4e00\u7d1a\u6297\u9ad4<\/a><\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

1.Salomonsen J, Dunon D, Skj\u00f8dt K, Thorpe D, Vainio O, Kaufman J. Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system. Proc Natl Acad Sci USA. 1991;88:1359-63. (IP, IHC-FS, FC)<\/p>\n

2.Kaufman J, Skjoedt K, Salomonsen J, Simonsen M, Du Pasquier L, Parisot R, et al. MHC-like molecules in some nonmammalian vertebrates can be detected by some cross-reactive xenoantisera. J Immunol. 1990;144:2258-72. (IP, Caiman Reactivity)<\/p>\n

3.Salomonsen J, Marston D, Avila D, Bumstead N, Johansson B, Juul-Madsen H, et al. The properties of the single chicken MHC classical class II \u03b1 chain (B-LA) gene indicate an ancient origin for the DR\/E-like isotype of class II molecules. Immunogenetics. 2003;55:605-14. (Purification)<\/p>\n

4.Bader SR, Kothlow S, Trapp S, Schwarz SC, Philipp H, Weigend S, et al. Acute paretic syndrome in juvenile White Leghorn chickens resembles late stages of acute inflammatory demyelinating polyneuropathies in humans. J Neuroinflammation. 2010;7:7. (IHC-FS, FC)<\/p>\n

5.del Cacho E, Gallego M, Lee SH, Lillehoj HS, Quilez J, Lillehoj EP, et al. Induction of protective immunity against Eimeria tenella infection using antigen-loaded dendritic cells (DC) and DC-derived exosomes. Vaccine. 2011;29:3818-25. (EM)<\/p>\n

6.Ekino S, Riwar B, Kroese FG, Schwander EH, Koch G, Nieuwenhuis P. Role of environmental antigen in the development of IgG+ cells in the bursa of fabricius. J Immunol. 1995;155:4551-8. (FC)<\/p>\n

7.Katevuo K, Vainio O. Thymocyte emigration in the chicken: an over-representation of CD4+ cells over CD8+ in the periphery. Immunology. 1996;89:419-23. (FC)<\/p>\n

8.Madruga J, Koritschoner NP, Diebold SS, Kurz SM, Zenke M. Polarised expression pattern of focal contact proteins in highly motile antigen presenting dendritic cells. J Cell Sci. 1999;112:1685-96. (FC)<\/p>\n

9.Koritschoner NP, Madruga J, Knespel S, Blendinger G, Anzinger B, Otto A, et al. The nuclear orphan receptor TR4 promotes proliferation of myeloid progenitor cells. Cell Growth Differ. 2001;12:563-72. (FC)<\/p>\n

10.Buitenhuis AJ, Kjaer JB, Labouriau R, Juul-Madsen HR. Altered circulating levels of serotonin and immunological changes in laying hens divergently selected for feather pecking behavior. Poult Sci. 2006;85:1722-8. (FC)<\/p>\n

11.Juul-Madsen HR, Dalgaard TS, R\u00f8ntved CM, Jensen KH, Bumstead N. Immune response to a killed infectious bursal disease virus vaccine in inbred chicken lines with different major histocompatibility complex haplotypes. Poult Sci. 2006;85:986-98. (FC)<\/p>\n

12.Del Cacho E, Gallego M, Lillehoj HS, L\u00f3pez-Bernard F, S\u00e1nchez-Acedo C. Avian follicular and interdigitating dendritic cells: isolation and morphologic, phenotypic, and functional analyses. Vet Immunol Immunopathol. 2009;129:66-75. (FC)<\/p>\n

13.Janardhana V, Broadway MM, Bruce MP, Lowenthal JW, Geier MS, Hughes RJ, et al. Prebiotics modulate immune responses in the gut-associated lymphoid tissue of chickens. J Nutr. 2009;139:1404-9. (FC)<\/p>\n

14.Dalgaard T, Boving MK, Handberg K, Jensen KH, Norup LR, Juul-Madsen HR. MHC expression on spleen lymphocyte subsets in genetically resistant and susceptible chickens infected with Marek’s disease virus. Viral Immunol. 2009;22:321-7. (FC)<\/p>\n

15.Sunkara LT, Achanta M, Schreiber NB, Bommineni YR, Dai G, Jiang W, et al. Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression. PLoS One. 2011;6(11):e27225. (FC)<\/p>\n

16.Revajov\u00e1 V, Slaminkov\u00e1 Z, Gre\u0161\u00e1kov\u00e1 L, Levkut M. Duodenal morphology and immune responses of broiler chickens fed low doses of deoxynivalenol. Acta Vet. Brno. 2013;82:337-42. (FC)<\/p>\n

17.Chrz\u0105stek K, Piasecki T, Wieliczko A. Impact of CpG oligodeoxynucleotide stimulation on percentage of T and B cells in chicken. Pol J Vet Sci. 2013;16:551-4. (FC)<\/p>\n

18.Fernandes Filho T, F\u00e1varo C Jr, Ingberman M, Beir\u00e3o BC, Inoue A, Gomes L, et al. Effect of spray Escherichia coli vaccine on the immunity of poultry. Avian Dis. 2013;57:671-6. (FC)<\/p>\n

19.Vu Manh T, Marty H, Sibille P, Le Vern Y, Kaspers B, Dalod M, et al. Existence of conventional dendritic cells in Gallus gallus revealed by comparative gene expression profiling. J Immunol. 2014;192:4510-7. (FC)<\/p>\n

20.Olias P, Meyer A, Klopfleisch R, Lierz M, Kaspers B, Gruber AD. Modulation of the host Th1 immune response in pigeon protozoal encephalitis caused by Sarcocystis calchasi. Vet Res. 2013;44:10. (IHC-FS, Pigeon Reactivity)<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Mouse Anti-Chicken MHC Class II-PE\u6297\u9ad4 | \u8ca8\u865f8350-09 \u898f\u683c1.0 […]<\/p>\n","protected":false},"author":3,"featured_media":6176,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136,3],"tags":[1561],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24708"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24708"}],"version-history":[{"count":2,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24708\/revisions"}],"predecessor-version":[{"id":24710,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24708\/revisions\/24710"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/6176"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24708"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24708"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24708"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}