{"id":24202,"date":"2020-03-31T13:24:47","date_gmt":"2020-03-31T05:24:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24202"},"modified":"2026-07-06T16:04:31","modified_gmt":"2026-07-06T08:04:31","slug":"picoprobe15-pgdh%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a-15-pgdh-activity-assay-kit%e8%b2%a8%e8%99%9fk562","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24202","title":{"rendered":"PicoProbe\u212215-PGDH\u6d3b\u6027\u6e2c\u5b9a | 15-PGDH Activity Assay Kit\u8ca8\u865fK562 (ABCAM ab273327)"},"content":{"rendered":"

PicoProbe\u212215-PGDH\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09 | 15-PGDH Activity Assay Kit\u8ca8\u865f<\/strong>K562 (ABCAM ab273327)<\/span><\/a><\/span><\/p>\n

PicoProbe\u2122 15-PGDH Activity Assay Kit (Fluorometric)<\/p>\n

\u7522\u54c1\u4ecb\u7d39:. 15-\u7fa5\u57fa\u524d\u5217\u817a\u7d20\u812b\u6c2b\u9176\uff0815-Hydroxy prostaglandin dehydrogenase , 15-PGDH; EC\uff1a1.1.1.141\uff09\u662f\u524d\u5217\u817a\u7d20\u5206\u89e3\u4ee3\u8b1d(prostaglandin catabolism.)\u7684\u9650\u901f\u9176(rate-limiting enzyme)\u3002\u5b83\u5c07\u524d\u5217\u817a\u7d20\uff08prostaglandins , PG\uff09\u6c27\u5316\u70ba15-\u916e\u4ee3\u8b1d\u7269(15-keto metabolites)\u3002\u901a\u5e38\u8207PG\u76f8\u6bd4\uff0c15-\u916e\u4ee3\u8b1d\u7269(15-keto metabolites) \u7684\u751f\u7269\u6d3b\u6027(biological activity)\u964d\u4f4e\u3002 PGs\u662f\u7531\u82b1\u751f\u56db\u70ef\u9178(arachidonic acid)\u901a\u904e\u74b0\u6c27\u5408\u9176\uff08cyclooxygenase , COX\uff09\u751f\u6210\u7684\uff0c\u5728\u708e\u75c7\u53cd\u61c9(inflammatory responses)\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002\u6700\u8fd1\u7684\u7814\u7a76\u767c\u73fe15-PGDH\u53ef\u4ee5\u4f5c\u70baPG\u7684\u964d\u89e3\u9176(degrading enzyme)\uff0c\u4e26\u53ef\u4ee5\u6291\u5236\u816b\u7624(tumor suppressor)\u7684\u767c\u5c55\u3002\u56e0\u6b64\uff0c\u5c0d15-PGDH\u6d3b\u6027\u7684\u7814\u7a76\u53ef\u4ee5\u95e1\u660e\u548c\u95e1\u660e\u8207\u764c\u75c7\u767c\u5c55\u548c\u6cbb\u7642\u6709\u95dc\u7684\u672a\u77e5\u65b9\u9762\u3002\u5728BioVision\u768415-PGDH\u6aa2\u6e2c\u8a66\u5291\u76d2\u4e2d\uff0c15-PGDH\u6c27\u5316\u5f62\u6210\u5e95\u7269\u7684\u4e2d\u9593\u9ad4\u548cNADH\u3002 NADH\u7684\u6c27\u5316\u6e1b\u5c11\u4e86\u63a2\u91dd\u5728Ex \/ Em = 535\/587 nm\u8655\u7522\u751f\u87a2\u5149\u3002 15-PGDH\u7684\u6d3b\u6027\u8207\u87a2\u5149\u4fe1\u865f\u6210\u6b63\u6bd4\u3002 BioVision\u768415-PGDH\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\uff0c\u7c21\u55ae\uff0c\u9748\u654f\u548c\u53ef\u9760\u7684\u6e2c\u8a66\u65b9\u6cd5\uff0c\u9069\u7528\u65bc\u6aa2\u6e2c\u4f4e\u81f31 \u00b5U\u768415-PGDH\u6d3b\u6027\u3002<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n

\"Functional<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK562-100 (ABCAM ab273327)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Fluorescence (Ex\/Em 535\/587 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Application- The 15-PGDH fluorometric assay kit is a highly sensitive assay and can detect < 5 \u00b5U 12-PGDH activity (based on our unit definition) in a variety of samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 535\/587 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nEukaryotes<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\n\u2022 Measurement of 15-PGDH activity in various tissues\/cells.
\n\u2022 Analysis of prostaglandin catabolism in various cell types.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 Fast and convenient
\n\u2022 Sensitive assay for measuring 15-PGDH activity in various biological samples and the kit is also suitable for high throughput analyses.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 15-PGDH Assay Buffer
\n\u2022 PicoProbeTM (in DMSO)
\n\u2022 15-PGDH Developer
\n\u2022 ALDH Substrate (in DMSO)
\n\u2022 NADH Standard
\n\u2022 15-PGDH Positive Control<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08<\/strong>MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/strong>\u6aa2\u6e2c<\/strong><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

PicoProbe\u212215-PGDH\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\uff08\u87a2\u5149\u6cd5\uff09 | 15-PGDH Activity Assay […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1598],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24202"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24202"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24202\/revisions"}],"predecessor-version":[{"id":54928,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24202\/revisions\/54928"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24202"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24202"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24202"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}