{"id":24124,"date":"2020-03-22T11:01:20","date_gmt":"2020-03-22T03:01:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24124"},"modified":"2022-07-19T17:24:19","modified_gmt":"2022-07-19T09:24:19","slug":"ace2-orf-vector-abmgood%e8%b2%a8%e8%99%9f111670110000","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24124","title":{"rendered":"ACE2 ORF Vector| ABMGOOD\u8ca8\u865f111670110000"},"content":{"rendered":"

ACE2 ORF Vector|\u00a0<\/strong>\u8ca8\u865f111670110000<\/strong>\u00a0(1.0 \u00b5g DNA)<\/a><\/span><\/p>\n

Abmgood\u63d0\u4f9b\u4e00\u7cfb\u5217\u5e8f\u5217\u9a57\u8b49\u958b\u653e\u95b1\u8b80\u6846\uff08ORF\uff09\u5eab\uff0c\u5305\u62ec\u4eba(human)\uff0c\u5c0f\u9f20(mouse)\u548c\u5927\u9f20(rat)\uff0c\u53efinsertion\u81f3\u60a8\u9078\u64c7\u7684\u86cb\u767d\u8cea\u8868\u9054\u7cfb\u7d71(protein expression systems.)\u3002\u53bb\u96645’UTR\u548c3’UTR\uff0cORF\u63d2\u5165\u517c\u5bb9\u7684\u8868\u9054\u8f09\u9ad4(compatible expression vector) \u5f8c\u5373\u53ef\u5feb\u901f\u53ef\u9760\u5730\u8868\u9054\u86cb\u767d\u8cea\u3002\u4e5f\u53ef\u7528\u4f5cPCR\u64f4\u589e (PCR amplification experiments) \u5be6\u9a57\u4e2d\u967d\u6027\u5c0d\u7167(positive control)\u7684\u6a21\u677f(template)\u3002Abmgood\u63d0\u4f9b\u7684ORF\u5305\u542b\u4e00\u500b\u7a81\u8b8a\u7684\u7d42\u6b62\u5bc6\u78bc\u5b50(mutated stop codon)\uff0c\u5982\u679c\u53d7\u9ad4\u8f09\u9ad4\u4e2d\u5b58\u5728C\u672b\u7aef\u6a19\u7c64\u6216\u5831\u544a\u57fa\u56e0\uff0c\u5247\u5141\u8a31\u5176\u647b\u5165\u3002 abm\u7684ORF\u4e5f\u53ef\u4ee5\u4ee5\u5373\u7528\u578b\u86cb\u767d\u8868\u9054\u8f09\u9ad4(protein expression vectors<\/a>)\uff0c\u6162\u75c5\u6bd2\u8f09\u9ad4(lentiviral vectors<\/a>)\u6216\u5305\u88dd\u7684\u6162\u75c5\u6bd2(\u00a0lentivirus<\/a>)\u548c\u817a\u75c5\u6bd2(\u00a0adenovirus\u00a0<\/a>)\u4e4b\u67b6\u69cb\u597d\u4e4b\u8f09\u9ad4\u6216\u75c5\u6bd2\u6db2\u5f62\u5f0f\u63d0\u4f9b\u3002<\/p>\n

ACE2 ORF<\/strong>\u8f09\u9ad4\u4ecb\u7d39: ACE2 ORF Vector (Human) (pORF)\u8f09\u9ad4\uff1b\u00a0Accession Number\u00a0BC039902 (Insert size: 2418bp)\u00a0<\/strong>\u3002\u5728AflII\u548cEcoRV\u5207\u5272\u4f4d\u9ede\u4e4b\u9593\u4fdd\u7559\u4e86\u8a72\u57fa\u56e0\uff08\u9650\u5236\u6027\u9176\u5207\u65b9\u6cd5\uff09\u3002Inserts\u4e0d\u5305\u542b\u4efb\u4f55\u5167\u90e8AflII\u548cEcoRV cut site\uff0c\u53ef\u4ee5AflII\u548cEcoRV\u5207\u9664\u3002\u5728\u63d2\u5165\u7247\u6bb5\u5305\u542b\u9019\u4e9b\u5167\u90e8\u5207\u5272\u4f4d\u9ede\u7684\u60c5\u6cc1\u4e0b\uff0c\u53ef\u4ee5PCR\u64f4\u589e\u5e36\u6709\u512a\u9078\u9650\u5236\u6027\u9176\u5207\u4f4d\u9ede\u7684\u63d2\u5165\u7247\u6bb5\uff0c\u4ee5\u9032\u884csubcloning\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/th>\nACE2 ORF Vector (Human) (pORF)<\/td>\n<\/tr>\n
Accession Number<\/th>\nBC039902<\/td>\n<\/tr>\n
Description<\/th>\nThe ACE2 ORF Vector holds the gene (cloned by a restriction enzyme-independent method) between an AflII and EcoRV cut site. Inserts are flanked by and can be excised using AflII and EcoRV as long as inserts do not contain any internal AflII and EcoRV sites. In the case that the insert contains these internal cut sites, the end user can PCR amplify the insert with preferred restriction sites for subcloning.<\/td>\n<\/tr>\n
SKU<\/th>\n111670110000<\/td>\n<\/tr>\n
Cat.No<\/th>\n111670110000<\/td>\n<\/tr>\n
Gene Name<\/th>\nACE2<\/td>\n<\/tr>\n
Full Gene Name<\/th>\nangiotensin I converting enzyme (peptidyl-dipeptidase A) 2<\/td>\n<\/tr>\n
List of species<\/th>\nHuman<\/td>\n<\/tr>\n
Units<\/th>\n1.0 \u00b5g DNA<\/td>\n<\/tr>\n
Vector<\/th>\npORF<\/a><\/td>\n<\/tr>\n
System<\/th>\nNon-Viral Vector<\/td>\n<\/tr>\n
Insert Size<\/th>\n2418bp<\/td>\n<\/tr>\n
List of Markers<\/th>\nBacterial: Spectinomycin<\/td>\n<\/tr>\n
HGNC ID<\/th>\nHGNC:13557<\/td>\n<\/tr>\n
Appearance<\/th>\nClear Liquid<\/td>\n<\/tr>\n
Vector Size<\/th>\n2596bp<\/td>\n<\/tr>\n
Storage Buffer<\/th>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Storage<\/th>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n
Internal Leadtime<\/th>\nInquire<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u76f8\u95dc\u5546\u54c1<\/p>\n

Lentivirus\u6162\u75c5\u6bd2\u4f4e\u901f30\u5206\u7d14\u5316\u5957\u7d44(\u7d14\u5316)<\/a>\u00a0|\u00a0Lentivirus \u6162\u75c5\u6bd2\u9ad8\u6fc3\u5ea6\u7d14\u5316\u5957\u7d44(\u7d14\u5316)\u00a0<\/a>\u00a0|\u00a0Lentivirus\u75c5\u6bd2\u5b9a\u91cf\u5feb\u7be9\u7247(\u5b9a\u91cf)<\/a>\u00a0|\u00a0Lentivirus\u75c5\u6bd2\u4e00\u500b\u6b65\u9a5fQPCR\u5b9a\u91cf\u5957\u7d44(\u5b9a\u91cf)<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

ACE2 ORF Vector|\u00a0\u8ca8\u865f111670110000\u00a0(1.0 \u00b5g DNA) Abmgood\u63d0\u4f9b\u4e00 […]<\/p>\n","protected":false},"author":3,"featured_media":24012,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1612],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24124"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=24124"}],"version-history":[{"count":1,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24124\/revisions"}],"predecessor-version":[{"id":24126,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/24124\/revisions\/24126"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24012"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=24124"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=24124"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=24124"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}