{"id":24046,"date":"2020-03-14T17:39:30","date_gmt":"2020-03-14T09:39:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=24046"},"modified":"2022-07-19T17:26:31","modified_gmt":"2022-07-19T09:26:31","slug":"%e7%95%b0%e6%aa%b8%e6%aa%ac%e9%85%b8%e6%af%94%e8%89%b2%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk656-100","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=24046","title":{"rendered":"\u7570\u6ab8\u6aac\u9178\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK656-100"},"content":{"rendered":"

Home<\/strong><\/a> \u00a0 \u00a0<\/strong>Literature<\/strong><\/a>\u00a0 \u00a0<\/strong>Promotions<\/strong><\/a>\u00a0\u00a0<\/strong>Product Citations<\/strong><\/a><\/p>\n

\u7570\u6ab8\u6aac\u9178\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K656-100<\/a><\/span><\/p>\n

Isocitrate Colorimetric Assay Kit<\/em><\/strong>\u00a0<\/em><\/strong><\/p>\n

\u7522\u54c1\u63cf\u8ff0\uff1a<\/strong>\u7570\u6ab8\u6aac\u9178\uff08Isocitric acid , HOOC-CHOH-CH\uff08-COOH\uff09-CH 2 -COOH\uff09\u662fKrebs TCA\u5faa\u74b0(Krebs TCA cycle)\u7684\u4e2d\u9593\u9ad4\uff0c\u4f4d\u65bc\u6ab8\u6aac\u9178\u9e7d(citrate)\u548c\u03b1-\u916e\u620a\u4e8c\u9178(\u03b1-keto-glutarate)\u4e4b\u9593\u3002\u5b83\u662f\u4e59\u919b\u9178(glyoxylate)\u5206\u6d41\u5668\u5728\u690d\u7269\u548c\u4f4e\u7b49\u751f\u7269\u4e2d\u8d77\u4f5c\u7528\u7684\u5206\u652f\u9ede\u3002\u5728\u8a31\u591a\u6c34\u679c\u548c\u852c\u83dc\u4ee5\u53ca\u7531\u9019\u4e9b\u539f\u6599\u751f\u7522\u7684\u98df\u54c1\u4e2d\u767c\u73fe\u4e86\u7570\u6ab8\u6aac\u9178(Isocitrate)\u7684\u5927\u91cf\u6fc3\u5ea6\u3002\u5728TCA\u5faa\u74b0\u4e2d\uff0c\u7570\u6ab8\u6aac\u9178(Isocitrate)\u88ab\u7570\u6ab8\u6aac\u9178\u812b\u6c2b\u9176\uff08isocitrate dehydrogenase , IDH\uff09\u6c27\u5316\u70ba\u03b1-\u916e\u620a\u4e8c\u9178(\u03b1-ketoglutarate)\uff0c\u4e26\u7522\u751fNAD\uff08P\uff09H\u3002 NAD-IDH\u7684\u55aa\u5931\u88ab\u8a8d\u70ba\u662f\u8272\u7d20\u6027\u8996\u7db2\u819c\u708e(retinitis pigmentosa)\u7684\u6f5b\u5728\u75c5\u56e0\u3002 BioVision\u7684\u7570\u6ab8\u6aac\u9178\u6e2c\u5b9a\u8a66\u5291\u76d2(Isocitrate Assay Kit, K656)\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\uff0c\u9748\u654f\uff0c\u5feb\u901f\u7684\u5b9a\u91cf\u5404\u7a2e\u6a23\u54c1\u4e2d\u7570\u6ab8\u6aac\u9178\u7684\u65b9\u6cd5\u3002\u5728\u6e2c\u5b9a\u4e2d\uff0c\u7570\u6ab8\u6aac\u9178(Isocitrate)\u88abNADPH\u6c27\u5316\uff0c\u5c07\u5e7e\u4e4e\u7121\u8272\u7684\u63a2\u91dd\u8f49\u8b8a\u70ba\u03bbmax\u70ba450nm\u7684\u5f37\u70c8\u8457\u8272\u7684\u7269\u8cea\u3002\u7b49\u6ab8\u6aac\u9178\u9e7d\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u6aa2\u6e2c1\u81f320\u7d0d\u6469\u723e\uff08\u301c0.2 \u2013 5 \u00b5g\uff09\u7684\u7570\u6ab8\u6aac\u9178\u9e7d\u3002\u6b64\u7522\u54c1\u9700\u7531\u5100\u5668\u9032\u884c\u5b9a\u91cf\u00a0<\/strong>ELISA<\/u><\/strong>\u5100\u5668\u8a2d\u5099\u8acb\u6309\u6b64<\/u><\/strong><\/a><\/span><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK656-100<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nAbsorbance (450 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nIsocitrate Assay Kit can detect 1 to 20 nmoles (~0.2 \u2013 5 \u00b5g) of isocitrate.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 40 minutes
\n\u2022 Fast and convenient
\n\u2022 Kit contains all necessary reagents for accurate measurement of isocitrate levels<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Isocitrate Assay Buffer
\n\u2022 Isocitrate Enzyme Mix
\n\u2022 Substrate Mix
\n\u2022 Isocitrate Standard (100 mM)<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a>\u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

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