{"id":23957,"date":"2020-03-11T13:01:54","date_gmt":"2020-03-11T05:01:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23957"},"modified":"2022-07-19T17:28:52","modified_gmt":"2022-07-19T09:28:52","slug":"mfei%e9%85%b5%e7%b4%a0-%e8%b2%a8%e8%99%9f-e057","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=23957","title":{"rendered":"MfeI\u9175\u7d20 | \u8ca8\u865f E057 20000 U\/ml (500 \u03bcl)"},"content":{"rendered":"

MfeI<\/strong> | <\/strong>\u8ca8\u865f<\/strong> E057<\/strong>\u00a0 \u00a020000 U\/ml\u00a0 (500 \u03bcl)\u00a0 \u8fa8\u8996C^AATTG\u5e8f\u5217\u00a0 \u00a0<\/strong><\/a><\/span><\/p>\n

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\u7522\u54c1\u4ecb\u7d39:<\/strong> MfeI\u9175\u7d20\u8fa8\u8996C^AATTG\u5e8f\u5217 (double-stranded DNA)\uff0c\u4f86\u6e90\u70ba\u651c\u5e36\u4f86\u81ea\u767c\u9175\u652f\u539f\u9ad4\u7684\u4fee\u98fe\u7684MfeI\u57fa\u56e0\u7684\u5927\u8178\u687f\u83cc\u83cc\u682a\uff08Mycoplasma fermentas (N.F.Halden)\u3002 \u5132\u5b58\u7de9\u885d\u6db210mM Tris pH\uff087.5\uff09\uff0c50mM NaCl\uff0c1mM DTT\uff0c0.1mM EDTA\u548c50\uff05\uff08v \/ v\uff09\u7518\u6cb9\u3002\u55ae\u4f4d\u5b9a\u7fa9:<\/strong> \u5b9a\u7fa9\u4e00\u500b\u55ae\u4f4d\u70ba\u6d88\u53161 \u00b5g\u03bbDNA\u6240\u9700\u7684\u9650\u5236\u9176\u91cf\uff0c\u572837\u21031\u5c0f\u6642\u5167\uff0c\u53cd\u61c9\u9ad4\u7a4d\u70ba50 \u00b5l\u3002\u5132\u5b58\u689d\u4ef6\u5c07\u6240\u6709\u7d44\u4ef6\u5b58\u653e\u5728-20\u00b0C\u4e0b\u3002\u907f\u514d\u6240\u6709\u7d44\u4ef6\u91cd\u8907\u9032\u884c\u51cd\u878d\u5faa\u74b0\u4fdd\u6301\u6700\u4f73\u6027\u80fd\u3002\u53cd\u61c9\u689d\u4ef6:<\/strong> 1X\u901a\u7528\u9650\u5236\u6027\u5167\u5207\u9176\u53cd\u61c9\u7de9\u885d\u6db2\uff08\u8ca8\u865fE204\uff09\uff0c\u65bc37\u00b0C\u5b75\u80b2\u3002\u53cd\u61c9\u7de9\u885d\u6db2\u517c\u5bb9\u6027\uff1a\u3000\u7de9\u885d\u53401\uff08\u8ca8\u865fE201\uff09\uff1a75\uff05\uff0c\u7de9\u885d\u53402\uff08\u8ca8\u865fE202\uff09\uff1a25\uff05\uff0c\u7de9\u885d\u53403\uff08\u8ca8\u865fE203\uff09\uff1a<10\uff05\uff0c\u901a\u7528\u7de9\u885d\u5340\uff08\u8ca8\u865fE204\uff09\uff1a100\uff05\u3002<\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n
PRODUCT<\/th>\nCAT NO<\/th>\n<\/th>\n<\/tr>\n<\/thead>\n
10X Restriction Enzyme Reaction Buffer 1<\/a><\/td>\nE201<\/td>\n<\/td>\n<\/tr>\n
10X Restriction Enzyme Reaction Buffer 2<\/a><\/td>\nE202<\/td>\n<\/td>\n<\/tr>\n
10X Restriction Enzyme Reaction Buffer 3<\/a><\/td>\nE203<\/td>\n<\/td>\n<\/tr>\n
10X Universal Restriction Enzyme Reaction Buffer<\/a><\/td>\nE204<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5176\u5b83<\/strong>\u5546\u54c1<\/strong>AKT2<\/u><\/strong>\u00a0<\/u>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/a> | <\/strong>EGFR<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>HER2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>LIMK1<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>MEK1 (EE)<\/u><\/strong>\u00a0<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a> | <\/strong>\u00a0<\/u><\/strong>ROCK2<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a> | <\/strong>ROS1 (G2032R)<\/u><\/strong>\u6d3b\u6027\u86cb\u767d<\/u><\/strong><\/a><\/p>\n

\"\"<\/p>\n

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MfeI | \u8ca8\u865f E057\u00a0 \u00a020000 U\/ml\u00a0 (500 \u03bcl)\u00a0 \u8fa8\u8996C^AATTG\u5e8f\u5217\u00a0 \u00a0 \u7522 […]<\/p>\n","protected":false},"author":3,"featured_media":2474,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[88],"tags":[1653],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23957"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23957"}],"version-history":[{"count":3,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23957\/revisions"}],"predecessor-version":[{"id":23961,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23957\/revisions\/23961"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2474"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23957"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23957"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23957"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}