{"id":23794,"date":"2020-02-20T17:11:16","date_gmt":"2020-02-20T09:11:16","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23794"},"modified":"2023-10-18T13:46:42","modified_gmt":"2023-10-18T05:46:42","slug":"%e8%83%83%e8%9b%8b%e7%99%bd%e9%85%b6%e8%83%83%e8%9b%8b%e7%99%bd%e9%85%b6%e5%8e%9f%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fk446","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=23794","title":{"rendered":"\u80c3\u86cb\u767d\u9176\/\u80c3\u86cb\u767d\u9176\u539f\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK446 (ABCAM, ab239722)"},"content":{"rendered":"

\u80c3\u86cb\u767d\u9176\/\u80c3\u86cb\u767d\u9176\u539f\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | <\/strong>\u8ca8\u865f<\/strong>K446\u00a0 (ABCAM, ab239722) <\/a><\/span><\/p>\n

Pepsin\/Pepsinogen Activity Assay Kit (Fluorometric)<\/p>\n

\"\"\"\"\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39 \u80c3\u86cb\u767d\u9176\uff08Pepsin , EC 3.4.23.1\uff09\u662f\u80bd\u9176A1\u5bb6\u65cf(peptidase A1 family)\u7684\u5929\u51ac\u6c28\u9178\u86cb\u767d\u9176(aspartic protease)\uff0c\u5728\u54fa\u4e73\u52d5\u7269(mammalian)\u7684\u80c3\u4e2d\u8d77\u4e3b\u8981\u7684\u6d88\u5316\u9176\u4f5c\u7528\u3002\u80c3\u86cb\u767d\u9176(Pepsin)\u5728\u80c3\u7c98\u819c\u7684\u4e3b\u8981\u7d30\u80de\u4e2d\u88ab\u5408\u6210\u70ba\u7121\u6d3b\u6027\u7684\u524d\u86cb\u767d\u9176\u80c3\u86cb\u767d\u9176\u539f\uff0c\u5b83\u542b\u6709\u4e00\u500b44\u500b\u6c28\u57fa\u9178\u7684\u81ea\u6291\u5236\u57df(autoinhibitory domain)\u3002\u5c07\u8a72\u539f\u9176\u5305\u88dd\u6210\u9846\u7c92(granules)\u4e26\u5132\u5b58\u5728\u80c3\u7c98\u819c\u7d30\u80de\u5167(gastric mucosal cells)\u3002\u98df\u7528\u98df\u7269\u5f8c\uff0c\u6cbf\u8457\u8ff7\u8d70\u795e\u7d93\u50b3\u64ad\u7684\u795e\u7d93\u5143\u4fe1\u865f\u89f8\u767cHCl\u548c\u80c3\u86cb\u767d\u9176\u539f\u5411\u80c3\u4e2d\u7684\u5206\u6ccc\u3002\u5728\u80c3\u7684\u9ad8\u9178\u6027\u74b0\u5883\u4e2d\uff0c\u80c3\u86cb\u767d\u9176\u539f\u5c55\u958b\u4e26\u9032\u884c\u81ea\u50ac\u5316\u88c2\u89e3(autocatalytic cleavage)\uff0c\u5f62\u6210\u6d3b\u6027\u86cb\u767d\u9176\u80c3\u86cb\u767d\u9176(active protease pepsin)\u3002\u5e7d\u9580\u87ba\u687f\u83cc\u611f\u67d3\u6703\u5f15\u8d77\u80c3\u9178(stomach acid)\u548c\u80c3\u86cb\u767d\u9176(pepsin)\u7684\u904e\u5ea6\u5206\u6ccc\uff0c\u5f9e\u800c\u5c0e\u81f4\u80c3\u6f70\u760d\u548c\u5341\u4e8c\u6307\u8178\u6f70\u760d(duodenal ulcers)\u7684\u5f62\u6210\u3002\u53e6\u5916\uff0c\u5728\u6162\u6027\u80c3\u98df\u7ba1\u53cd\u6d41\u75be\u75c5(chronic gastroesophageal reflux disease)\u4e2d\u89c0\u5bdf\u5230\u904e\u91cf\u7684\u80c3\u86cb\u767d\u9176\u53ef\u80fd\u5728\u98df\u7ba1\u548c\u5589\u7d44\u7e54\u7684\u964d\u89e3\u4e2d\u8d77\u4f5c\u7528\u3002\u96d6\u7136\u5927\u591a\u6578\u80c3\u86cb\u767d\u9176\u539f\u5728\u80c3\u7c98\u819c\u4e2d\u8868\u9054\uff0c\u4f46\u5c11\u91cf\u7684\u60f0\u6027\u9176\u4e5f\u91cb\u653e\u5230\u8840\u6db2\u4e2d\u3002\u8840\u6e05\u80c3\u86cb\u767d\u9176\u539f\u6c34\u5e73\u8207\u80c3\u7c98\u819c\u529f\u80fd\u6539\u8b8a\u76f8\u95dc\uff0c\u901a\u5e38\u7528\u65bc\u7be9\u67e5\u80c3\u764c\u548c\u6162\u6027\u5e7d\u9580\u87ba\u687f\u83cc\u8a98\u767c\u7684\u80c3\u708e\u3002 BioVision\u7684\u80c3\u86cb\u767d\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2\u662f\u4e00\u7a2e\u540c\u8cea\u6e2c\u5b9a\u6cd5(homogenous assay)\uff0c\u53ef\u7528\u65bc\u91cf\u5316\u80c3\u7d44\u7e54\u548c\u5404\u7a2e\u751f\u7269\u9ad4\u6db2\u4e2d\u7684\u80c3\u86cb\u767d\u9176\u6d3b\u6027\u3002\u8a72\u6e2c\u5b9a\u5229\u7528\u4e86\u5e36\u6709\u87a2\u5149\u5718\u548c\u87a2\u5149\u731d\u6ec5\u5291\u7684\u5408\u6210\u80bd\u5e95\u7269\u3002\u7d93\u80c3\u86cb\u767d\u9176\u5207\u5272\u5f8c\uff0c\u672a\u731d\u6ec5\u5e36\u6709\u87a2\u5149\u5718\u7684\u80bd\u7247\u6bb5\u4ee5\u7522\u751f\u660e\u4eae\u7684\u87a2\u5149\u4fe1\u865f\uff08Ex \/ Em = 328\/418 nm\uff09\u3002\u80bd\u9176A1\u5bb6\u65cf\u7684\u6eb6\u9176\u9ad4\u5929\u51ac\u6c28\u9178\u86cb\u767d\u9176\uff08\u7d44\u7e54\u86cb\u767d\u9176D\u548cE\uff09\u4e0d\u6703\u5e72\u64fe\u6e2c\u5b9a\u3002\u8a72\u6e2c\u5b9a\u5feb\u901f\uff0c\u6613\u65bc\u57f7\u884c\uff0c\u4e26\u4e14\u6bd4\u7d93\u5178\u7684\u8840\u7d05\u86cb\u767d\u964d\u89e3\u6e2c\u5b9a\u9748\u654f\u5f97\u591a\uff0c\u6bcf\u5b54\u7684\u6aa2\u6e2c\u6975\u9650\u70ba500\u03bcU\u80c3\u86cb\u767d\u9176\u6d3b\u6027\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK446-100 (ABCAM, ab239722)<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n100 assays<\/td>\n<\/tr>\n
Kit Summary<\/strong><\/td>\n\u2022 Detection method- Fluorescence (Ex\/Em: 328\/418 nm)
\n\u2022 Species reactivity- Mammalian
\n\u2022 Application- The kit detects as low as 500 \u00b5U pepsin in various samples.<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nFluorescence (Ex\/Em 328\/418 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nEstimation of pepsin activity in various biological samples<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure; takes ~ 1-2 hours based on pepsin levels
\n\u2022 Fast and convenient<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Pepsin Assay Buffer
\n\u2022 Pepsin Substrate
\n\u2022 Pepsin Inhibitor (Pepstatin A)
\n\u2022 Pepsin Positive Control
\n\u2022 MCA Standard<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Wendy Xin, Oligodendrocytes Support Neuronal Glutamatergic Transmission via Expression of Glutamine Synthetase.\u00a0Cell Rep., May 2019; 31116973.<\/a><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u80c3\u86cb\u767d\u9176\/\u80c3\u86cb\u767d\u9176\u539f\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u8ca8\u865fK446\u00a0 (ABCAM, ab239722) Pepsin\/Pe […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1683],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23794"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23794"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23794\/revisions"}],"predecessor-version":[{"id":46205,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23794\/revisions\/46205"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23794"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23794"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23794"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}