{"id":23065,"date":"2020-01-15T14:27:56","date_gmt":"2020-01-15T06:27:56","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=23065"},"modified":"2022-07-19T17:39:40","modified_gmt":"2022-07-19T09:39:40","slug":"%e7%9c%9f%e8%8f%8c%e8%9b%8b%e7%99%bd%e7%b4%b0%e8%83%9e%e5%a3%81%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92detergent-free-invent%e8%b2%a8%e8%99%9fyt-015","status":"publish","type":"post","link":"https:\/\/biopioneer.com.tw\/?p=23065","title":{"rendered":"\u5fae\u751f\u7269 (\u7d30\u80de\u58c1) \u86cb\u767d\u8403\u53d6| Invent biological\u8ca8\u865fYT-015"},"content":{"rendered":"

\u7522\u54c1\u540d\u7a31<\/strong>:<\/strong> \u771f\u83cc\u86cb\u767d(\u7d30\u80de\u58c1)\u63d0\u53d6\u8a66\u5291\u76d2(detergent-free) | Invent<\/strong>\u8ca8\u865f<\/strong>YT-015<\/strong><\/a><\/span><\/p>\n

Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Preps)<\/p>\n

 <\/p>\n

\"\"<\/p>\n

\u9069\u7528\u65bc\u9175\u6bcd(yeast)\uff0c\u7d72\u72c0\u771f\u83cc(filamentous fungus)\uff0c\u9769\u862d\u6c0f\u967d\u6027(gram-positive)\u548c\u9769\u862d\u6c0f\u9670\u6027\u7d30\u83cc(negative bacteria)\uff0c\u6606\u87f2\u5375(insect eggs)\uff0c\u5fae\u85fb\u985e(microalgae)\u7b49\u86cb\u767d\u8cea\u8403\u53d6<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/strong> \u542b\u6709\u7d30\u80de\u58c1\u7684\u5fae\u751f\u7269(cell-wall-containing microbes) \u4e2d\u63d0\u53d6\u86cb\u767d\u8cea\u662f\u4e00\u7a2e\u5e38\u898b\u7a0b\u5e8f\u3002YT-015\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7121\u9700\u5100\u5668\uff0c\u5feb\u901f\u800c\u6eab\u548c\u7684\u65b9\u5f0f\uff0c\u53ef\u5f9e\u5177\u6709\u539a\u800c\u5805\u56fa\u7684\u7d30\u80de\u58c1\u7684\u5fae\u751f\u7269 (microbes) \u4e2d\u63d0\u53d6\u86cb\u767d\u8cea\u3002\u9019\u4e9b\u5fae\u751f\u7269\u5305\u62ec\u9175\u6bcd(yeast)\uff0c\u7d72\u72c0\u771f\u83cc(filamentous fungus)\uff0c\u9769\u862d\u6c0f\u967d\u6027(gram-positive)\u548c\u9769\u862d\u6c0f\u9670\u6027\u7d30\u83cc(negative bacteria)\uff0c\u6606\u87f2\u5375(insect eggs)\uff0c\u5fae\u85fb\u985e(microalgae)\u7b49\u3002\u8a72\u8a66\u5291\u76d2\u5305\u542b\u512a\u5316\u7684\u8b8a\u6027(denaturing protein extraction buffers \u8ca8\u865f) \u548c\u5929\u7136\u86cb\u767d\u8cea\u63d0\u53d6\u7de9\u885d\u6db2 (native protein extraction buffers)\u4f9b\u60a8\u9078\u64c7\u3002\u8207\u8a31\u591a\u5176\u4ed6\u82db\u523b\u689d\u4ef6\u4e0b\u7684\u65b9\u6cd5\u4e0d\u540c\uff08\u4f8b\u59828 M\u5c3f\u7d20\uff09\uff0c\u4f7f\u7528\u9175\u6bcd\u9032\u884c\u5100\u5668\u9032\u884c\u73bb\u7483\u73e0\u88c2\u89e3\u4e26\u7528\u9e7c\u8403\u53d6\u5c07\u6a23\u54c1\u716e\u6cb8\u7b49\u65b9\u6cd5\u7528\u65bc\u9175\u6bcd\u86cb\u767d\u8cea\u8403\u53d6\u3002\u901a\u5e38\u4f7f\u7528\u9019\u4e9b\u65b9\u6cd5\u512a\u5148\u63d0\u53d6\u67d0\u4e9b\u5206\u5b50\u91cf\u7bc4\u570d\u5167\u7684\u86cb\u767d\u8cea\u3002\u8a72\u8a66\u5291\u76d2\u5177\u6709\u55ae\u7ba1\u64cd\u4f5c\u898f\u7a0b\uff0c\u53ef\u4ee5\u7121\u504f\u5dee\u5730\u63d0\u53d6\u6240\u6709\u7a2e\u985e\u7684\u9175\u6bcd\u86cb\u767d\u3002\u8a72\u8a66\u5291\u76d2\u9069\u7528\u65bc\u5c0d\u6578\u671f(log-phase)\u548c\u56fa\u5b9a\u671f(stationary-phase)\u4e4b\u5fae\u751f\u7269\u3002\u6574\u500b\u904e\u7a0b\u4e0d\u523010\u5206\u9418\u5373\u53ef\u5b8c\u6210\uff0c\u86cb\u767d\u8cea\u7522\u91cf\u57282-5 mg \/ ml\u7684\u7bc4\u570d\u5167\u3002\u63d0\u4f9b\u7684\u6750\u6599\u8db3\u4ee5\u9032\u884c50\u6b21\u53cd\u61c9\u3002<\/p>\n

\"\"<\/p>\n

Minute\u2122<\/strong>\u86cb\u767d\u8403\u53d6\u5957\u7d44\uff0c\u4f7f\u7528\u5275\u65b0\u300e<\/strong>Column Filter<\/strong>\u300f\u6280\u8853\uff0c\u6548\u679c\u66f4\u4f73\uff0c\u5b8c\u6210\u53ea\u9700<\/strong>\u00a0<\/strong>\u4e00<\/strong> \u5206\u9418\u86cb\u767d\u8403\u53d6\u53ea\u9700\u8981<\/strong>vortex<\/em><\/strong>\u3001<\/em><\/strong>transfer<\/em><\/strong>\u3001<\/em><\/strong>spin <\/em><\/strong>\u3002<\/em><\/strong>Minute \u2122\u4e00\u7cfb\u5217\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\uff1b\u9069\u7528\u65bc\u591a\u7a2e\u7269\u7a2e\uff08\u5305\u62ec\u54fa\u4e73\u52d5\u7269\u3001\u6d77\u6d0b\u52d5\u7269\u3001\u6606\u87f2\u3001\u690d\u7269\u548c\u7d30\u83cc\uff09\u5feb\u901f\u548c\u5bb9\u6613\u5730\u63d0\u53d6\/\u5206\u96e2\u86cb\u767d\u8cea\u7684\u512a\u7570\u5de5\u5177 \u3002\u8207\u5e02\u5834\u4e0a\u7684\u4efb\u4f55\u5176\u4ed6\u86cb\u767d\u8cea\u63d0\u53d6\u8a66\u5291\u76d2\u4e0d\u540c\uff0c\u512a\u5316\u7684\u7de9\u885d\u6db2\u7cfb\u7d71\uff0c\u7d50\u5408\u6700\u65b0\u7684\u5c08\u6709\u6ffe\u7b52\u6280\u8853\uff08spin column\uff09\uff1b\u5feb\u901f\u63d0\u9ad8\u86cb\u767d\u8403\u53d6\u7684\u6548\u80fd\u53ca\u65b9\u4fbf\u6027\u3002\u7121\u9808\u9577\u6642\u9593\u7b49\u5f85\u3001\u514d\u9664\u91cd\u8907\u6027\u7684wash\u6b65\u9a5f\u3002<\/p>\n

Kit includes:<\/strong><\/p>\n\n\n\n\n\n\n\n\n
Items<\/strong><\/td>\nQuantity<\/strong><\/td>\n<\/tr>\n
Denaturing Buffer<\/td>\n20 ml<\/td>\n<\/tr>\n
Native Buffer<\/td>\n20 ml<\/td>\n<\/tr>\n
Protein Extraction Powder<\/td>\n5 grams<\/td>\n<\/tr>\n
1.5 ml Microfuge Tube<\/td>\n50 units<\/td>\n<\/tr>\n
Pestles<\/td>\n4 units<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/u><\/td>\nCatalog No.<\/u><\/td>\n<\/tr>\n
\u3000<\/u><\/td>\n\u3000<\/u><\/td>\n<\/tr>\n
Minute TM<\/sup> Total Protein Extraction Kit\u00a0 for Animal Cultured Cells\/Tissues (50 Tests)<\/td>\nSD-001\/SN-002<\/td>\n<\/tr>\n
Minute TM<\/sup> Cytoplasmic and Nuclear Fractionation kit (50 Tests)<\/td>\nSC-003<\/td>\n<\/tr>\n
Minute TM<\/sup> Detergent-Free Total Protein Extraction Kit for Animal Cells\/Tissues (50 Tests)<\/td>\nSN-006<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit (50 Tests)<\/td>\nSM-005<\/td>\n<\/tr>\n
Minute TM<\/sup> Plasma Membrane Protein Isolation Kit for Plants (50 Tests)<\/td>\nSM-005-p<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Mammalian Cells and Tissues(50 Tests)<\/td>\nMP-007<\/td>\n<\/tr>\n
Minute TM <\/sup>Mitochondria Isolation Kit for Muscle Tissues\/Cultured Muscle Cells (50 Tests) NEW!<\/td>\nMM-038<\/td>\n<\/tr>\n
Minute TM <\/sup>Total Protein Extraction Kit for Plant Tissues (50 Tests)<\/td>\nSD-008\/SN-009<\/td>\n<\/tr>\n
Minute TM <\/sup>Detergent-Free Plant Protein Extraction Kit (50 Tests)<\/td>\nSN-010<\/td>\n<\/tr>\n
Minute TM<\/sup> Chloroplast Isolation Kit (50 Tests)<\/td>\nCP-011<\/td>\n<\/tr>\n
Minute TM <\/sup>Bacterial Total Protein Extraction Kit (50 Tests)<\/td>\nSB-004<\/td>\n<\/tr>\n
Minute TM<\/sup> Single Cell Isolation Kit (50 Tests)<\/td>\nSC-012<\/td>\n<\/tr>\n
Minute TM<\/sup> Nuclear Envelope Protein Extraction Kit (50 Tests)<\/td>\nNE-013<\/td>\n<\/tr>\n
Minute TM<\/sup> Histon\/DNA Binding\u00a0 Protein Extraction Kit (50 Tests)<\/td>\nHP-014<\/td>\n<\/tr>\n
Minute\u2122 Total Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYT-015<\/td>\n<\/tr>\n
Minute\u2122 Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls (50 Tests)<\/td>\nYD-016<\/td>\n<\/tr>\n
Minute\u2122 Yeast Mitochondria Enrichment Kit (50 tests)<\/td>\nYM-017<\/td>\n<\/tr>\n
Minute\u2122 Plant Microsomal Membrane Extraction Kit (50 Tests)<\/td>\nMM-018<\/td>\n<\/tr>\n
Minute\u2122 Protein\/Nucleic Acid Extraction Kit from Gels (20 tests)<\/td>\nPN-019<\/td>\n<\/tr>\n
MinuteTM Protein Extraction Kit for Hair and Nails (20 Tests)<\/td>\nHD-021<\/td>\n<\/tr>\n
MinuteTM Total Protein Extraction Kit for Adipose Tissues\/Cultured Adipocytes (20 Tests)<\/td>\nAT-022<\/td>\n<\/tr>\n
MinuteTM Adipose Tissue Fractionation Kit (20 Tests)<\/td>\nAF-023<\/td>\n<\/tr>\n
MinuteTM <\/sup>Detergent-Free Nuclei Isolation Kit (20 Tests)<\/td>\nNI-024<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Neuronal Tissues\/Cells (20 preps) NEW!<\/td>\nBN-020<\/td>\n<\/tr>\n
MinuteTM <\/sup>Single Nucleus Isolation Kit for Tissues\/Cells (20 preps) NEW!<\/td>\nSN-047<\/td>\n<\/tr>\n
MinuteTM<\/sup> Protein Extraction Kit for Fixed and Embedded Tissues (20 Tests)<\/td>\nFE-025<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Exosome Isolation Reagent (20 ml) (Non-PEG)<\/td>\nEI-027<\/td>\n<\/tr>\n
MinuteTM<\/sup> Hi-Efficiency Saliva Exosome Isolation Kit (50 Tests) (Non-PEG)<\/td>\nSE-030<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic and Nuclear Extraction Kit for Frozen\/Fresh Tissues (20 tests)<\/td>\nNT-032<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lysosome Isolation Kit (20 tests)<\/td>\nLY-034<\/td>\n<\/tr>\n
MinuteTM<\/sup> ER Enrichment Kit\u00a0 (20 tests)<\/td>\nER-036<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-039<\/td>\n<\/tr>\n
MinuteTM<\/sup> Lipid Raft Isolation Kit for Mammalian Cells\/Tissues (20 tests) NEW!<\/td>\nLR-042<\/td>\n<\/tr>\n
MinuteTM<\/sup> Cytosolic Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPT-040<\/td>\n<\/tr>\n
MinuteTM<\/sup> Nuclear Proteasome Enrichment Kit (20 tests) NEW!<\/td>\nPN-041<\/td>\n<\/tr>\n
MinuteTM<\/sup> Plant Cytosolic and Nuclear Protein Isolation Kit (20 tests) NEW!<\/td>\nPF-045<\/td>\n<\/tr>\n
MinuteTM<\/sup> Native Protein Precipitation Kit from Saliva (20 tests) NEW!<\/td>\nSV-043<\/td>\n<\/tr>\n
MinuteTM<\/sup> Organic Solvent-Free Milk Lipid Depletion Kit (20 tests) NEW!<\/td>\nML-044<\/td>\n<\/tr>\n
MinuteTM<\/sup> Rubisco Depletion Kit (20 tests) NEW!<\/td>\nRD-046<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Skin Tissue (50 tests)<\/td>\nSA-01-SK<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Bone Tissue (50 tests)<\/td>\nSA-02-BT<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Blood Vessels (50 tests)<\/td>\nSA-03-BV<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Tendons (50 tests)<\/td>\nSA-04-TD<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Intestines and Mesentery (50 tests)<\/td>\nSA-05-IM<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Muscles (50 tests)<\/td>\nSA-06-MS<\/td>\n<\/tr>\n
MinuteTM <\/sup>Total Protein Extraction Kit for Insects (50 tests)<\/td>\nSA-07-IS<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u86cb\u767d\u8cea\u8403\u53d6\u71b1\u9580\u5546\u54c1 \u8102\u80aa\u7d44\u7e54\u86cb\u767d\u8cea\u8403\u53d6\u5957\u7d44<\/a> | \u8cea\u819c\u86cb\u767d\u8403\u53d6\u5957\u7d44<\/a> |<\/p>\n

\u5f15\u7528\u6587\u737b<\/p>\n

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  1. Nagayoshi, Y., Miyazaki, T., Shimamura, S., Nakayama, H., Minematsu, A., Yamauchi, S., … & Mukae, H. (2017). Unexpected effects of azole transporter inhibitors on antifungal susceptibility in Candida glabrata and other pathogenic Candida species. PloS one, 12(7), e0180990.<\/li>\n
  2. Al-Omari, A., Griffith, J., Caranica, C., Taha, T., Schuttler, H. B., & Arnold, J. (2018). Discovering regulators in Post-Transcriptional control of the Biological Clock of Neurospora crassa using Variable Topology Ensemble Methods on GPUs.\u00a0IEEE access<\/em>.<\/li>\n
  3. Terashima, M., Ohashi, K., Takasuka, T. E., Kojima, H., & Fukui, M. Antarctic heterotrophic bacterium Hymenobacter nivis P3T displays light\u2010enhanced growth and expresses putative photoactive proteins.\u00a0Environmental Microbiology Reports<\/em>.<\/li>\n
  4. Lin, W., Yuan, D., Deng, Z., Niu, B., & Chen, Q. The cellular and molecular mechanism of glutaraldehyde\u2010didecyldimethylammonium bromide as a disinfectant against Candida albicans.\u00a0Journal of Applied Microbiology<\/em>.<\/li>\n
  5. Shimamura, S., Miyazaki, T., Tashiro, M., Takazono, T., Saijo, T., Yamamoto, K., … & Mukae, H. (2019). Autophagy-Inducing Factor Atg1 Is Required for Virulence in the Pathogenic Fungus Candida glabrata.\u00a0Frontiers in microbiology<\/i>,\u00a010<\/i>.<\/li>\n
  6. Wang, Y., Ma, Y., Wang, D., Liu, W., Chen, J., Jiang, Y., … & Qin, L. (2019). Polar tube structure and three polar tube proteins identified from Nosema pernyi.\u00a0Journal of Invertebrate Pathology<\/em>, 107272.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u7522\u54c1\u540d\u7a31: \u771f\u83cc\u86cb\u767d(\u7d30\u80de\u58c1)\u63d0\u53d6\u8a66\u5291\u76d2(detergent-free) | Invent\u8ca8\u865fYT-015 M […]<\/p>\n","protected":false},"author":3,"featured_media":4646,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[97],"tags":[1730],"_links":{"self":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23065"}],"collection":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=23065"}],"version-history":[{"count":4,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23065\/revisions"}],"predecessor-version":[{"id":28156,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/23065\/revisions\/28156"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/4646"}],"wp:attachment":[{"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=23065"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=23065"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=23065"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}